阅读说明：本技术 一种利用比色试剂现场快速检测毒品中***的方法 (Method for rapidly detecting cannabis in drugs on site by utilizing colorimetric reagent ) 是由 窦新存 张天实 于 2020-06-05 设计创作，主要内容包括：本发明提供了一种利用比色试剂现场快速检测毒品中大麻的方法,该检测方法所述比色试剂由2,6-二氯醌-4-氯亚胺,水和氢氧化钠配制而成,测试纸为普通滤纸,用棉棒采集样品,将样品置于测试纸上,将比色试剂滴加到含有样品的测试纸上,通过观察是否产生灰绿色复合物,以此确定大麻的存在。本发明所述的检测方法可用于现场毒品分析以及涉毒案件中对大麻类毒品的定性检测,检测限可达到10μg。该方法无须将可疑物样品溶解,具有操作简单迅速,特异性强,反应灵敏,使用方便,成本低的特点,可为公安系统刑侦部门涉毒案件的侦破提供有效技术手段。(The invention provides a method for rapidly detecting cannabis in drugs on site by utilizing a colorimetric reagent, which is prepared from 2, 6-dichloroquinone-4-chloroimine, water and sodium hydroxide, wherein test paper is common filter paper, a cotton swab is used for collecting a sample, the sample is placed on the test paper, the colorimetric reagent is dripped on the test paper containing the sample, and the existence of cannabis is determined by observing whether a grayish green compound is generated or not. The detection method can be used for field drug analysis and qualitative detection of cannabis drugs in drug-involved cases, and the detection limit can reach 10 mu g. The method does not need to dissolve suspicious samples, has the characteristics of simple and rapid operation, strong specificity, sensitive reaction, convenient use and low cost, and can provide an effective technical means for the detection of drug-involved cases in the criminal investigation department of the public security system.)

1. A method for rapidly detecting cannabis in drugs on site by utilizing colorimetric reagents is characterized by comprising the following operation steps:

a. weighing 0.005-0.01g of 2, 6-dichloroquinone-4-chloroimine at room temperature, adding 20mL of absolute ethanol, fully stirring until the absolute ethanol is completely dissolved, sealing, introducing nitrogen for 20min, and packaging a detection reagent in a reagent bottle to obtain a detection reagent A;

b. weighing 0.04g of sodium hydroxide, adding water, stirring until the sodium hydroxide is fully dissolved, then fixing the volume to a 100mL volumetric flask to obtain 0.01mol/L sodium hydroxide solution, and encapsulating the sodium hydroxide solution in a reagent bottle to obtain a detection reagent B;

c. b, extracting a detection sample from the object to be detected by using a cotton swab, wiping the detection sample on test paper, dropwise adding the detection reagent A obtained in the step a onto the test paper containing the detection sample, dropwise adding the detection reagent B, and observing color change to determine whether hemp, hemp extract or other hemp products exist in the object to be detected.

Technical Field

The invention relates to a method for rapidly detecting cannabis in drugs on site by utilizing a colorimetric reagent, in particular to cannabis, cannabis extract or other cannabis products.

Background

In recent years, the development of a rapid, sensitive and accurate field drug detection technology has very important significance. At present, the commercialized field drug detection means comprise ion mobility spectrometry, Raman spectroscopy, olfactory dog, fluorescence method and the like, but from the aspects of sensitivity and portability, the ion mobility spectrometry has the defects of time consumption in calibration and higher cost; portable raman techniques generally require large sample volumes and expensive instruments; the olfactory poisoning dog is also influenced by factors such as emotion and the like, and is difficult to continuously and stably detect.

Cannabis is a common drug with complex components, and tetrahydrocannabinol in cannabis is believed to increase the release of nucleus accumbens dopamine, resulting in a potentiating effect. Cannabinoids have also been found to interact with a number of neurotransmitters, including gamma-aminobutyric acid, glutamic acid, choline and the like. Cannabis mainly acts on the human body through cannabinoid receptors, which are G protein-coupled receptors and can inhibit the activity of adenylate cyclase through inhibitory G proteins, thereby lowering intracellular cyclic adenosine levels, and further, cannabinoid receptors can regulate ion channels, causing a decrease in neurotransmitter release in presynaptic membrane neurons, thereby causing corresponding excitatory or inhibitory effects on postsynaptic membranes. The main hazards are: (1) the cannabinol effect causes tachycardia, the heart rate is increased by 20-50 percent, and the maximum can reach 140 and 150 times/minute; (2) the long-term use causes the damage of the functions of the lung and the upper respiratory tract, and can cause diseases such as tracheitis, pharyngitis, edema of the larynx and the like; (3) cannabinol can impair cellular regulation and humoral regulation, reducing the ability to resist bacterial and viral infections; (4) excessive intake of cannabis results in relaxation of muscle tone and dysfunction of the balance; (5) excessive intake of cannabis can cause unconsciousness, anxiety, depression, etc., which are easy to generate hostile impulsion or suicide will; (6) the abuse of marijuana can cause the damage of large-scale cognitive function, and can reduce short-term brain memory, thinking, attention and judgment, and make people slow in thinking, pina and disordered memory. Because cannabis is easily available and exists in part of national regions even legally, detection and control of cannabis is an important link in handling drug-related cases.

At present, the cannabis detection methods mainly comprise two types, one type is azo salt dyes, and the azo salt dyes can generate condensation reaction with phenolic substances in cannabis under an acidic or alkaline environment to generate color change. The method utilizes diazotization reaction, but the specificity depends on the formation of conjugated molecules, and amine substances with similar structures can also react with the conjugated molecules and cannot be distinguished. And the other method is to qualitatively analyze phenolic substances by utilizing the Folin-Ciocalteu method and utilizing the oxidation reaction of phosphomolybdate-phosphotungstate on phenol. Chinese patent: CN105021608 uses the optimized Folin-Ciocalteu method to detect the total polyphenol content of the beverage. However, the method has long color development time, reacts with all phenols, and is not suitable for on-site rapid detection of cannabis, and the detection result is easy to interfere.

The invention provides a method for rapidly detecting cannabis in drugs on site by utilizing a colorimetric reagent, which mainly utilizes 2, 6-dichloroquinone-4-chloroimine to carry out condensation reaction with tetrahydrocannabinol, the main component of which is phenolic hydroxyl group and is not substituted, in cannabis sativa to generate a grey-green compound to determine the existence of the tetrahydrocannabinol, thereby realizing the detection of cannabis sativa. The colorimetric reagent can perform color reaction with phenolic compounds which are not substituted at the para-position of the phenolic hydroxyl group, but the color can generate different colors according to the structures of the compounds (for example, the colorimetric reagent reacts with phenol derivatives to generate bright blue). The reagent has the advantages of easily obtained raw materials, simple and convenient preparation, light weight, portability, strong selectivity and wide application potential.

Disclosure of Invention

The invention aims to provide a method for rapidly detecting cannabis in drugs on site by utilizing a colorimetric reagent, which is based on a chemical colorimetric method, does not need to dissolve a suspicious sample, and utilizes 2, 6-dichloroquinone-4-chloroimine to react with tetrahydrocannabinol in cannabis to generate a gray-green compound so as to achieve the effect of detecting cannabis. The detection method can be used for field drug analysis and qualitative detection of cannabis drugs in drug-involved cases, has the characteristics of simple and rapid operation, strong specificity, sensitive response, convenient use and low cost, and can provide an effective technical means for detecting drug-involved cases in public security system criminal investigation departments.

The invention relates to a method for rapidly detecting cannabis in drugs on site by utilizing a colorimetric reagent, which comprises the following operation steps:

a. weighing 0.005-0.01g of 2, 6-dichloroquinone-4-chloroimine at room temperature, adding 20mL of absolute ethanol, fully stirring until the absolute ethanol is completely dissolved, sealing, introducing nitrogen for 20min, and packaging a detection reagent in a reagent bottle to obtain a detection reagent A;

b. weighing 0.04g of sodium hydroxide, adding water, stirring until the sodium hydroxide is fully dissolved, then fixing the volume to a 100mL volumetric flask to obtain 0.01mol/L sodium hydroxide solution, and encapsulating the sodium hydroxide solution in a reagent bottle to obtain a detection reagent B;

c. b, extracting a detection sample from the object to be detected by using a cotton swab, wiping the detection sample on test paper, dropwise adding the detection reagent A obtained in the step a onto the test paper containing the detection sample, dropwise adding the detection reagent B, and observing color change to determine whether hemp, hemp extract or other hemp products exist in the object to be detected.

The invention relates to a method for rapidly detecting cannabis in drugs on site by utilizing a colorimetric reagent, which comprises the steps of taking the colorimetric reagent prepared from 2, 6-dichloroquinone-4-chloroimine, sodium hydroxide and water as the detection reagent at room temperature, collecting a sample by using a cotton stick, placing the sample on test paper, dropwise adding the detection reagent on the test paper containing the sample, and reacting the 2, 6-dichloroquinone-4-chloroimine with tetrahydrocannabinol in the cannabis to generate a gray-green compound so as to determine the existence of the cannabis; if the color is gray green, the object to be detected is proved to contain the hemp; if the color is not gray green, the object to be tested is proved to contain no cannabis.

The method for rapidly detecting the cannabis in the drugs on site by using the colorimetric reagent can detect at least 10 mu g of cannabis.

Detailed Description