阅读说明：本技术 基于微流控盘式芯片的微量试剂消耗水质氨氮检测方法 (Micro-reagent consumption water ammonia nitrogen detection method based on micro-fluidic disc chip ) 是由 张倩叶 武治国 潘凌 何增益 张春萍 周久 张振扬 陈银 徐锦锋 于 2020-06-22 设计创作，主要内容包括：本发明公开了基于微流控盘式芯片的微量试剂消耗水质氨氮检测方法,分别对酒石酸钾钠试剂和纳氏试剂进行冻干处理；将冻干处理完成后的酒石酸钾钠球状试剂和纳氏球状试剂分别置入微流控盘式芯片对应的第一反应腔和第二反应腔中；将待测水样加入到微流控盘式芯片,最后在微流控盘式芯片的比色腔生成待测物；通过分光光度计进行氨氮检测。本发明试剂用量小。试剂对环境依赖性低,可大大延长存储时间,利于保存,操作简单。(The invention discloses a micro-reagent consumption water ammonia nitrogen detection method based on a micro-fluidic disc chip, which comprises the steps of respectively carrying out freeze-drying treatment on a potassium sodium tartrate reagent and a nano reagent; respectively placing the potassium sodium tartrate spherical reagent and the nano-scale spherical reagent which are subjected to freeze-drying treatment into a first reaction cavity and a second reaction cavity corresponding to the microfluidic disc chip; adding a water sample to be detected into the microfluidic disc chip, and finally generating an object to be detected in a colorimetric cavity of the microfluidic disc chip; and detecting ammonia nitrogen by a spectrophotometer. The invention has small dosage of the reagent. The reagent has low dependence on the environment, can greatly prolong the storage time, is beneficial to preservation and is simple to operate.)

1. The method for detecting ammonia nitrogen in water consumed by micro-reagent based on the micro-fluidic disc chip is characterized by comprising the following steps:

step 1: freeze-drying the potassium sodium tartrate reagent, and curing to obtain a potassium sodium tartrate spherical reagent;

step 2: carrying out freeze-drying treatment on the nano-scale reagent, and solidifying the nano-scale reagent into a nano-scale spherical reagent;

and step 3: respectively placing the potassium sodium tartrate spherical reagent and the nano spherical reagent which are subjected to freeze-drying treatment into a first reaction cavity and a second reaction cavity corresponding to the microfluidic disc chip in advance;

and 4, step 4: controlling the temperature of the microfluidic disc chip to be 20-60 ℃;

and 5: acquiring a water sample to be detected, and adding the water sample to be detected into the microfluidic disk chip;

step 6: a water sample to be detected sequentially flows through the first reaction cavity and the second reaction cavity at a set flow rate, and finally a substance to be detected is generated in a colorimetric cavity of the microfluidic disc type chip;

and 7: emitting a full-spectrum signal through an LED lamp of a spectrophotometer to irradiate a colorimetric cavity of the microfluidic disc type chip, and converting absorbed light with the wavelength of 420nm into an electric signal by a photoelectric detection module of the spectrophotometer;

and 8: and carrying out data processing and analysis according to the converted electric signal to obtain final ammonia nitrogen detection data.

2. The method for detecting ammonia nitrogen in water consumed by micro-reagent based on the micro-fluidic disc chip according to claim 1, wherein the water sample to be detected in the step 5 is diluted and then added into the micro-fluidic disc chip.

Technical Field

The invention relates to the field of water quality monitoring, in particular to a micro-reagent consumption water ammonia nitrogen detection method based on a micro-fluidic disc chip, which is suitable for monitoring methods requiring addition of chemical reagents, spectrophotometry and the like.

Background

Since the 70 s in the 20 th century, China has achieved unprecedented achievement in the aspect of water environment protection, and the trend of rapid deterioration of water environment pollution is primarily controlled, but the current water environment pollution condition is still very severe, and water pollution becomes a global problem. At present, common indexes for water quality detection include items such as pH, total phosphorus, total nitrogen, COD, ammonia nitrogen and the like. Wherein the ammonia nitrogen causes pollution to water, so that fishes die, aquatic organisms are subjected to acute ammonia nitrogen poisoning, or nitrite is formed to harm the health of human beings.

The method is used for measuring nitrogen compounds in various forms in water, and is helpful for evaluating the pollution and self-purification conditions of the water body. The source of ammonia nitrogen in water is mainly decomposed products of nitrogenous organic matters in domestic sewage under the action of microorganisms, and some industrial waste water, such as coking waste water, waste water of synthetic ammonia fertilizer plants, farmland drainage and the like.

At present, common methods for testing ammonia nitrogen in water include a Nassner reagent spectrophotometry method, a salicylic acid spectrophotometry method, a distillation neutralization titration method, an electrode method and the like. The common detection method of the surface water is a nano reagent spectrophotometry and a salicylic acid spectrophotometry.

Principle of the Nassner reagent method: ammonia nitrogen existing in the form of free ammonia or ammonium ions reacts with the nano reagent to generate a brown complex, the absorbance of the brown complex is in direct proportion to the content of ammonia nitrogen, and the brown complex is measured by a spectrophotometer at the 420nm position.

The prior laboratory method and on-line detection have the following defects:

1. high environmental requirement, short effective period of reagent and no storage benefit.

2. The manual maintenance workload is large, and the curve needs to be corrected and the reagent needs to be updated regularly.

3. Has higher professional ability for operators and can not realize fool operation.

4. The reagent dosage is large, and the test cost is high.

5. The amount of waste liquid generated is large, and a professional company is required to process the waste liquid, so that the operation cost of the company is increased.

Disclosure of Invention

The invention aims to provide a micro-reagent consumption water quality ammonia nitrogen detection method based on a micro-fluidic disc type chip aiming at the defects and shortcomings in the prior art, and solves the problems that the existing water quality ammonia nitrogen detection method is complicated in detection process, high in requirement on operator specialty, large in reagent consumption, large in wastewater output, environment-friendly, short in reaction reagent validity period and not beneficial to storage.

The purpose of the invention is realized by the following technical scheme:

the method for detecting ammonia nitrogen in water consumed by micro-reagent based on the micro-fluidic disc chip comprises the following steps:

step 1: freeze-drying the potassium sodium tartrate reagent, and curing to obtain a potassium sodium tartrate spherical reagent;

step 2: carrying out freeze-drying treatment on the nano-scale reagent, and solidifying the nano-scale reagent into a nano-scale spherical reagent;

and step 3: respectively placing the potassium sodium tartrate spherical reagent and the nano spherical reagent which are subjected to freeze-drying treatment into a first reaction cavity and a second reaction cavity corresponding to the microfluidic disc chip in advance;

and 4, step 4: controlling the temperature of the microfluidic disc chip to be 20-60 ℃;

and 5: acquiring a water sample to be detected, and adding the water sample to be detected into the microfluidic disk chip;

step 6: a water sample to be detected sequentially flows through the first reaction cavity and the second reaction cavity at a set flow rate, and finally a substance to be detected is generated in a colorimetric cavity of the microfluidic disc type chip;

and 7: emitting a full-spectrum signal through an LED lamp of a spectrophotometer to irradiate a colorimetric cavity of the microfluidic disc type chip, and converting absorbed light with the wavelength of 420nm into an electric signal by a photoelectric detection module of the spectrophotometer;

and 8: and carrying out data processing and analysis according to the converted electric signal to obtain final ammonia nitrogen detection data.

And (3) diluting the water sample to be detected in the step (5) and adding the diluted water sample to the microfluidic disk chip.

Compared with the prior art, the invention has the following advantages:

1. and a micro-fluidic disc type chip is adopted, so that the reagent consumption is small.

2. The micro reaction reagent, the potassium sodium tartrate reagent and the Nashi reagent are subjected to freeze-drying treatment, so that the method has low dependence on the environment, can greatly prolong the storage time and is beneficial to storage.

3. And (3) pretreating a reaction reagent, solidifying the reagent amounts of the potassium sodium tartrate reagent and the Nassner reagent which participate in the reaction, and shortening the operation time of the detection reaction.

4. The method does not need to have higher professional ability on the requirement of operators, is easy to operate, and can finish the detection of the ammonia nitrogen in the water quality only by adding a water sample.

5. The reagent dosage is small, and the test cost is low.

6. Almost no waste liquid is generated, and no professional company is required to treat the waste liquid. The detection process is more environment-friendly, and the environmental treatment cost is further reduced.

Detailed Description

The present invention will be described in further detail with reference to examples for the purpose of facilitating understanding and practice of the invention by those of ordinary skill in the art, and it is to be understood that the present invention has been described in the illustrative embodiments and is not to be construed as limited thereto.

The method for detecting ammonia nitrogen in water consumed by micro-reagent based on the micro-fluidic disc chip comprises the following steps:

step 1: freeze-drying the potassium sodium tartrate reagent, and solidifying the potassium sodium tartrate reagent into the potassium sodium tartrate spherical reagent with fixed mass

Step 2: the nano-scale reagent is subjected to freeze-drying treatment and solidified into a nano-scale reagent with fixed mass.

And step 3: and respectively placing the potassium sodium tartrate spherical reagent and the nano-scale spherical reagent which are subjected to freeze-drying treatment into a first reaction cavity and a second reaction cavity corresponding to the microfluidic disc chip in advance.

And 4, step 4: and controlling the temperature of the microfluidic disc chip to be 20-60 ℃.

And 5: and obtaining a water sample to be tested, diluting the water sample to be tested according to a set dilution ratio, adding the diluted water sample to the microfluidic disk chip, diluting the diluted water sample by using a proportional dilution instrument, and adding the diluted water sample to be tested into the microfluidic disk chip according to the volume required by the microfluidic disk chip.

Step 6: the diluted water sample to be detected sequentially flows through a first reaction cavity (potassium sodium tartrate spherical reagent) and a second reaction cavity (Nas spherical reagent) at a set flow rate, the flow rate is set so that the water sample to be detected fully reacts in the first reaction cavity and the second reaction cavity in sequence, and finally, a substance (complex) to be detected is generated in a colorimetric cavity of the microfluidic disc type chip.

And 7: and an LED lamp of the spectrophotometer emits a full-spectrum signal to irradiate the colorimetric cavity of the microfluidic disc type chip. The photodetector module of the spectrophotometer converts the absorbed light at a wavelength of 420nm into an electrical signal.

And 8: and carrying out data processing and analysis according to the converted electric signal to obtain final ammonia nitrogen detection data.

The specific embodiments described herein are merely illustrative of the spirit of the invention. Various modifications or additions may be made to the described embodiments or alternatives may be employed by those skilled in the art without departing from the spirit or ambit of the invention as defined in the appended claims.