阅读说明：本技术 基于微流控盘式芯片的微量试剂消耗水质余氯检测方法 (Micro-reagent-consumption water quality residual chlorine detection method based on micro-fluidic disc chip ) 是由 张倩叶 武治国 潘凌 何增益 张春萍 周久 张振扬 陈银 徐锦锋 于 2020-06-22 设计创作，主要内容包括：本发明公开了基于微流控盘式芯片的微量试剂消耗水质余氯检测方法,分别对磷酸盐缓冲溶液和DPD干粉进行冻干处理；将冻干处理完成后的磷酸盐缓冲球状试剂和DPD干粉球状试剂分别置入微流控盘式芯片对应的第一反应腔和第二反应腔中；将待测水样加入到微流控盘式芯片,最后在微流控盘式芯片的比色腔生成待测物；通过分光光度计进行余氯检测。本发明试剂用量小。试剂对环境依赖性低,可大大延长存储时间,利于保存,操作简单。(The invention discloses a micro-reagent consumption water quality residual chlorine detection method based on a micro-fluidic disc chip, which comprises the steps of respectively carrying out freeze-drying treatment on a phosphate buffer solution and DPD dry powder; respectively placing the phosphate buffer spherical reagent and the DPD dry powder spherical reagent which are subjected to freeze-drying treatment into a first reaction cavity and a second reaction cavity corresponding to the microfluidic disc chip; adding a water sample to be detected into the microfluidic disc chip, and finally generating an object to be detected in a colorimetric cavity of the microfluidic disc chip; residual chlorine detection is carried out by a spectrophotometer. The invention has small dosage of the reagent. The reagent has low dependence on the environment, can greatly prolong the storage time, is beneficial to preservation and is simple to operate.)

1. The method for detecting the residual chlorine of the water quality consumed by the micro-reagent based on the micro-fluidic disc chip is characterized by comprising the following steps of:

step 1: freeze-drying the phosphate buffer solution, passing and solidifying the phosphate buffer solution into a phosphate buffer spherical reagent,

step 2: the DPD dry powder is freeze-dried and solidified into a spherical reagent of the DPD dry powder,

and step 3: respectively placing the phosphate buffer spherical reagent and the DPD dry powder spherical reagent which are subjected to freeze-drying treatment into a first reaction cavity and a second reaction cavity corresponding to the microfluidic disc chip,

and 4, step 4: controlling the temperature of the micro-fluidic disc chip to be 20-60 ℃,

and 5: acquiring a water sample to be detected, adding the water sample to be detected into the microfluidic disk chip,

step 6: a water sample to be detected sequentially flows through the first reaction cavity and the second reaction cavity at a set flow rate, and finally a substance to be detected is generated in the colorimetric cavity of the microfluidic disc type chip,

and 7: the LED lamp of the spectrophotometer emits full-spectrum signals to irradiate a colorimetric cavity of the microfluidic disc type chip, a photoelectric detection module of the spectrophotometer converts the absorbed light with the wavelength of 515nm into electric signals,

and 8: and carrying out data processing and analysis according to the converted electric signals to obtain final residual chlorine detection data.

2. The method for detecting the residual chlorine in the consumed water quality of the micro-reagent based on the micro-fluidic disc chip as claimed in claim 1, wherein the water sample to be detected in the step 5 is diluted and then added into the micro-fluidic disc chip.

Technical Field

The invention relates to the field of tap water and drinking water monitoring, in particular to a micro-reagent water consumption residual chlorine detection method based on a micro-fluidic disc chip, which is suitable for monitoring methods requiring addition of chemical reagents, spectrophotometry and the like.

Background

With the rapid development of social economy and urbanization, the problems of drinking water safety guarantee, water pollution prevention and control, water environment protection, water resource comprehensive management and the like which are ubiquitous in developing countries are increasingly highlighted. In most developing countries, the form of water resources is severe, the phenomenon of direct sewage discharge is very common, and the serious problem of drinking water safety exists. At present, common indexes for detecting the quality of drinking water comprise items such as PH, turbidity, TDS, residual chlorine and the like. The residual chlorine is an important index for testing whether the drinking water reaches the standard.

At present, common methods for testing ammonia nitrogen in water include an electrode method, a DPD spectrophotometry method and a tetramethylbenzidine visual colorimetry.

Principle of DPD method: DPD reacts rapidly with free residual chlorine in water to give a red color, measured at 515 nm.

The prior laboratory method and on-line detection have the following defects:

1. the requirement on environment is high, the effective period of the standard solution reagent is short, and the storage is not facilitated.

2. Has higher professional ability for operators and can not realize fool operation.

3. The electrode is not easy to calibrate and the calibration liquid has poor stability.

4. The electrode has short use and maintenance period, serious drift in the use process and poor accuracy.

Disclosure of Invention

The invention aims to provide a micro-flow control disc type chip-based detection method for residual chlorine in water quality consumed by micro-reagent, aiming at overcoming the defects and shortcomings in the prior art, and solves the problems of complicated laboratory detection process, higher requirement on the professional performance of operators and poor electrode detection accuracy in the existing detection method for ammonia nitrogen in water quality

The purpose of the invention is realized by the following technical scheme:

the method for detecting the residual chlorine of the water consumed by the micro-reagent based on the micro-fluidic disc chip comprises the following steps:

step 1: freeze-drying the phosphate buffer solution, passing and solidifying the phosphate buffer solution into a phosphate buffer spherical reagent,

step 2: the DPD dry powder is freeze-dried and solidified into a spherical reagent of the DPD dry powder,

and step 3: respectively placing the phosphate buffer spherical reagent and the DPD dry powder spherical reagent which are subjected to freeze-drying treatment into a first reaction cavity and a second reaction cavity corresponding to the microfluidic disc chip,

and 4, step 4: controlling the temperature of the micro-fluidic disc chip to be 20-60 ℃,

and 5: acquiring a water sample to be detected, adding the water sample to be detected into the microfluidic disk chip,

step 6: a water sample to be detected sequentially flows through the first reaction cavity and the second reaction cavity at a set flow rate, and finally a substance to be detected is generated in the colorimetric cavity of the microfluidic disc type chip,

and 7: the LED lamp of the spectrophotometer emits full-spectrum signals to irradiate a colorimetric cavity of the microfluidic disc type chip, a photoelectric detection module of the spectrophotometer converts the absorbed light with the wavelength of 515nm into electric signals,

and 8: and carrying out data processing and analysis according to the converted electric signals to obtain final residual chlorine detection data.

And (3) diluting the water sample to be detected in the step (5) and adding the diluted water sample to the microfluidic disk chip.

Compared with the prior art, the invention has the following advantages:

1. and a micro-fluidic disc type chip is adopted, so that the reagent consumption is small.

2. The micro reaction reagent, the phosphate buffer solution and the DPD dry powder are subjected to freeze-drying treatment, so that the environmental dependence is low, the storage time can be greatly prolonged, and the preservation is facilitated.

3. And (3) pretreating a reaction reagent, solidifying the reagent amount of the phosphate buffer solution and the DPD dry powder participating in the reaction, and shortening the operation time of the detection reaction.

4. The method does not need to have higher professional ability on the requirement of operators, is easy to operate, and can finish the detection of the ammonia nitrogen in the water quality only by adding a water sample.

5. The reagent dosage is small, and the test cost is low.

6. Almost no waste liquid is generated, and no professional company is required to treat the waste liquid. The detection process is more environment-friendly, and the environmental treatment cost is further reduced.

Detailed Description

The present invention will be described in further detail with reference to examples for the purpose of facilitating understanding and practice of the invention by those of ordinary skill in the art, and it is to be understood that the present invention has been described in the illustrative embodiments and is not to be construed as limited thereto.

A micro-reagent consumption water residual chlorine detection method based on a micro-fluidic disc chip comprises the following steps:

step 1: and (3) carrying out freeze-drying treatment on the phosphate buffer solution, and passing and solidifying the phosphate buffer solution to obtain the phosphate buffer spherical reagent with set mass.

Step 2: and (3) carrying out freeze-drying treatment on the DPD dry powder, and solidifying to obtain the DPD dry powder spherical reagent with set quality.

And step 3: and respectively placing the phosphate buffer spherical reagent and the DPD dry powder spherical reagent which are subjected to freeze-drying treatment into a first reaction cavity and a second reaction cavity corresponding to the microfluidic disc chip in advance.

And 4, step 4: controlling the temperature of the micro-fluidic disc chip to be 20-60 DEG C

And 5: and obtaining a water sample to be tested, diluting the water sample to be tested according to a set dilution ratio, adding the diluted water sample to the microfluidic disk chip, diluting the diluted water sample by using a proportional dilution instrument, and adding the diluted water sample to be tested into the microfluidic disk chip according to the volume required by the microfluidic disk chip.

Step 6: the diluted water sample to be detected sequentially flows through a first reaction cavity (phosphate buffer spherical reagent) and a second reaction cavity (DPD dry powder spherical reagent) at a set flow rate, the flow rate is set so that the water sample to be detected fully reacts in the first reaction cavity and the second reaction cavity in sequence, and finally, a substance to be detected (a red compound) is generated in a colorimetric cavity of the microfluidic disc type chip.

And 7: and an LED lamp of the spectrophotometer emits a full-spectrum signal to irradiate the colorimetric cavity of the microfluidic disc type chip. The photodetector module of the spectrophotometer converts the absorbed light at a wavelength of 515nm into an electrical signal.

And 8: and carrying out data processing and analysis according to the converted electric signals to obtain final residual chlorine detection data.

The specific embodiments described herein are merely illustrative of the spirit of the invention. Various modifications or additions may be made to the described embodiments or alternatives may be employed by those skilled in the art without departing from the spirit or ambit of the invention as defined in the appended claims.