阅读说明：本技术 哌嗪类新精神活性物质的显色检验方法 (Color development test method of piperazine novel mental active substance ) 是由 孟梁 黄振城 于 2020-07-29 设计创作，主要内容包括：本发明公开一种哌嗪类新精神活性物质的显色检验方法,涉及新精神活性物质的鉴定领域。本方法使用西门试剂、香荚兰素试剂、马改氏试剂和硝酸-硫酸试剂,对已知种类的哌嗪类新精神活性物质进行显色反应,并建立显色检验筛查程序图,再依据显色检验筛查程序图,利用该4种显色试剂对待检测的未知种类的哌嗪类新精神活性物质进行种类鉴别。本发明建立22种哌嗪类新精神活性物质的显色检验方法,该方法简单、便捷、所用试剂少,无需使用大型设备和复杂仪器,只需事先配制好4种显色试剂(西门试剂、香荚兰素试剂、马改氏试剂和硝酸-硫酸试剂),利用试管、烧杯、载玻片、白瓷板或厚白纸板,就可对22种哌嗪类新精神活性物质进行现场快速检验。(The invention discloses a color development test method of piperazine novel psychoactive substances, and relates to the field of identification of novel psychoactive substances. The method comprises the steps of carrying out color development reaction on the known piperazine new psychoactive substances by using a Siemens reagent, a vanillin reagent, a maxhlet reagent and a nitric acid-sulfuric acid reagent, establishing a color development test screening program diagram, and carrying out type identification on the unknown piperazine new psychoactive substances to be detected by using the 4 color development reagents according to the color development test screening program diagram. The invention establishes a color development test method for 22 piperazine new psychoactive substances, which is simple, convenient and quick, uses less reagents, does not need large-scale equipment and complex instruments, only needs to prepare 4 color development reagents (Siemens reagent, vanillin reagent, Maji reagent and nitric acid-sulfuric acid reagent) in advance, and can carry out on-site quick test on the 22 piperazine new psychoactive substances by using a test tube, a beaker, a glass slide, a white porcelain plate or a thick white cardboard.)

1. A method for detecting the color development of piperazine novel psychotropic active substances is characterized in that: carrying out color reaction on the known piperazine new psychoactive substances by using 4 color developing reagents, establishing a color developing test screening program diagram, and carrying out species identification on the unknown piperazine new psychoactive substances to be detected by using the 4 color developing reagents according to the color developing test screening program diagram;

the known classes of novel psychoactive substances of the piperazine family include N-benzylpiperazine, 1-benzyl-4-methylpiperazine hydrochloride, 1, 4-dibenzylpiperazine, 1- (3-chlorobenzyl) piperazine, 4' -difluorobenzylpiperazine, 1-piperonylpiperazine, 1- (3-trifluoromethylphenyl) piperazine, 1- (4-fluorophenyl) piperazine, 1- (2, 4-difluorophenyl) piperazine, 1- (2-fluorophenyl) piperazine, 1- (3-chlorophenyl) piperazine, 1- (2, 3-dichlorophenyl) piperazine, 1- (3, 4-dichlorophenyl) piperazine, p-chlorophenylpiperazine, 1- (3-chlorophenyl) -4- (3-chloropropyl) piperazine, N-benzylpiperazine, 1, 4-benzylpiperazine hydrochloride, 1, 4-difluorophenylpiperazine, 1- (3-chlorophenyl) piperazine, N-benzylpiperazine hydrochloride, 1-4-methylpiperazine hydrochloride, 1, 4-difluoro, 1- (2-methoxyphenyl) piperazine, 1- (3-methoxyphenyl) piperazine, 1- (4-methoxyphenyl) piperazine, 1- (2-methylphenyl) piperazine, 1- (3-methylphenyl) piperazine, 1- (4-methylphenyl) piperazine, and N-phenylpiperazine;

the color reagent comprises a Siemens reagent, a vanillin reagent, a maxhlet reagent and a nitric acid-sulfuric acid reagent;

the step of testing the piperazine-based novel psychoactive substances of unknown species comprises: firstly, the Siemens reagent is respectively added into each piperazine new psychoactive substance of unknown species, and if the Siemens reagent corresponds to a single color recorded in a color development test screening program diagram, the species of the piperazine new psychoactive substance can be directly judged; and if the color is corresponding to the repeated color recorded in the color development examination screening program diagram, taking the new piperazine psychoactive substances which are not subjected to color development according to the repeated color, adding the vanillin reagent, the majiki reagent or the nitric acid-sulfuric acid reagent according to the color development examination screening program diagram, and sequentially identifying the type of each piperazine substance according to the color development result.

2. The method for color development test of piperazine-based novel psychoactive substances according to claim 1, wherein:

the Siemens reagent comprises two components of solution A and solution B, and is prepared by the following steps: dissolving 0.9g of sodium nitrosyl ferricyanide in 50-130 mL of water, adding 5-15 mL of acetaldehyde, and shaking up; and B, liquid B: dissolving 2g of sodium carbonate in 50-150 mL of water, and shaking up;

the vanillin reagent comprises three components of solution A, solution B and solution C, and is prepared by the following steps: dissolving 0.4g of vanillin in 15-25 mL of 95% ethanol, adding 0.1-1 mL of acetaldehyde, and shaking up; and B, liquid B: hydrochloric acid; and C, liquid C: chloroform;

the maxjestic reagent comprises a component and is prepared by the following steps of adding 10mL of 98% concentrated sulfuric acid into 0.5-1.5 mL of formaldehyde solution, and shaking up;

the nitric acid-sulfuric acid reagent comprises a component and is prepared by the following steps of adding 0.1-1 mL of nitric acid into 10mL of 98% concentrated sulfuric acid and shaking up.

3. The method for chromogenic test of piperazine-based novel psychoactive substances according to claim 2, wherein said chromogenic test screening program diagram is established by the steps of:

the siemens reagent solution a was added to 22 kinds of the known piperazine new psychoactive substances: n-phenylpiperazine, 1-benzyl-4-methylpiperazine hydrochloride, 1- (4-fluorophenyl) piperazine, 1- (3-methoxyphenyl) piperazine, N-phenylpiperazine, 1- (2, 4-difluorophenyl) piperazine, 1- (3-chlorobenzyl) piperazine, 1- (2-methylphenyl) piperazine, and 1- (3-methylphenyl) piperazine all appeared blue; the 1- (2-fluorophenyl) piperazine, the 1- (4-methylphenyl) piperazine, the 4,4' -difluorobenzylpiperazine, the 1- (2, 3-dichlorophenyl) piperazine, the p-chlorophenyl piperazine, the 1- (3-chlorophenyl) piperazine, the 1- (4-methoxyphenyl) piperazine, the 1- (3, 4-dichlorophenyl) piperazine and the 1- (3-chlorophenyl) -4- (3-chloropropyl) piperazine all show green; ③ 1- (2-methoxyphenyl) piperazine and 1-piperonyl piperazine show dark blue; the 1, 4-dibenzyl piperazine shows light yellow; fifthly, the 1- (3-trifluoromethyl phenyl) piperazine shows brown yellow;

then, adding a Siemens reagent solution A into each known piperazine novel mental active substance, and then adding a Siemens reagent solution B: n-phenylpiperazine, 1-benzyl-4-methylpiperazine hydrochloride, 1- (4-fluorophenyl) piperazine and 1- (3-methoxyphenyl) piperazine are blue; the N-phenylpiperazine is green, and is brownish red after fully reacting for 1-3 min; the 1- (2, 4-difluorophenyl) piperazine is purple, and is brown after fully reacting for 1-3 min; 1- (3-chlorobenzyl) piperazine exhibits a dark blue color; 1- (2-methylphenyl) piperazine and 1- (3-methylphenyl) piperazine are purple and are purple after fully reacting for 1-3 min; ② 1- (2-fluorophenyl) piperazine and 1- (4-methylphenyl) piperazine are purple, and are purple red after fully reacting for 1-3 min; 4,4' -difluorobenzylpiperazine, 1- (2, 3-dichlorophenyl) piperazine and p-chlorophenyl piperazine are blue; 1- (3-chlorophenyl) piperazine and 1- (4-methoxyphenyl) piperazine are brown; 1- (3, 4-dichlorophenyl) piperazine presents brown yellow; 1- (3-chlorophenyl) -4- (3-chloropropyl) piperazine appears yellow; ③ 1- (2-methoxyphenyl) piperazine and 1-piperonyl piperazine show dark blue, after fully reacting for 1-3 min, the 1- (2-methoxyphenyl) piperazine still shows dark blue, and the 1-piperonyl piperazine shows purple; fourthly, enabling the 1, 4-dibenzyl piperazine to be light yellow and to be brown after fully reacting for 1-3 min; fifthly, the 1- (3-trifluoromethyl phenyl) piperazine is brownish yellow;

according to the above process of adding the Siemens reagent A liquid and B liquid, N-phenylpiperazine, 1- (2, 4-difluorophenyl) piperazine, 1- (3-chlorobenzyl) piperazine, 1- (3, 4-dichlorophenyl) piperazine, 1- (3-chlorophenyl) -4- (3-chloropropyl) piperazine, 1- (2-methoxyphenyl) piperazine, 1-piperonyl piperazine, 1, 4-dibenzyl piperazine, and 1- (3-trifluoromethylphenyl) piperazine were added, and these 9 piperazines showed a single color and could be directly distinguished.

4. The method for color development test of piperazine novel mental active substances according to claim 3, wherein the establishment of the color development test screening program diagram further comprises the following steps of, on the basis of the identification of 9 piperazines by adding siemens reagent solution A and solution B, continuously adding a color developing agent to piperazine which does not show a single color, and performing identification, wherein the method comprises the following steps:

taking un-developed (i) N-phenylpiperazine, 1-benzyl-4-methylpiperazine hydrochloride, 1- (4-fluorophenyl) piperazine and 1- (3-methoxyphenyl) piperazine, respectively and continuously adding the vanillin reagent A liquid, the B liquid and the C liquid, and fully reacting for 1-3 min to obtain colorless N-phenylpiperazine and 1-benzyl-4-methylpiperazine hydrochloride, light yellow 1- (4-fluorophenyl) piperazine and pink 1- (3-methoxyphenyl) piperazine;

1- (4-fluorophenyl) piperazine and 1- (3-methoxyphenyl) piperazine show a single color and can be directly distinguished;

respectively adding the nitric acid-sulfuric acid reagent into the un-colored (i) N-phenylpiperazine and 1-benzyl-4-methylpiperazine hydrochloride, wherein the N-phenylpiperazine is light yellow, and the 1-benzyl-4-methylpiperazine hydrochloride is colorless;

the N-phenylpiperazine and the 1-benzyl-4-methylpiperazine hydrochloride show a single color and can be directly distinguished.

5. The method for color development test of piperazine novel mental active substances according to claim 3, wherein the establishment of the color development test screening program diagram further comprises the following steps of, on the basis of the identification of 9 piperazines by adding siemens reagent solution A and solution B, continuously adding a color developing agent to piperazine which does not show a single color, and performing identification, wherein the method comprises the following steps:

taking un-developed 1- (2-methylphenyl) piperazine and 1- (3-methylphenyl) piperazine, respectively and continuously adding the vanillin reagent A liquid, B liquid and C liquid, and reacting for 1-3 min, wherein the 1- (2-methylphenyl) piperazine is colorless, and the 1- (3-methylphenyl) piperazine is pink; 1- (2-methylphenyl) piperazine and 1- (3-methylphenyl) piperazine respectively have a single color and can be directly distinguished.

6. The method for color development test of piperazine novel mental active substances according to claim 3, wherein the establishment of the color development test screening program diagram further comprises the following steps of, on the basis of the identification of 9 piperazines by adding siemens reagent solution A and solution B, continuously adding a color developing agent to piperazine which does not show a single color, and performing identification, wherein the method comprises the following steps:

the non-developed 1- (2-fluorophenyl) piperazine and 1- (4-methylphenyl) piperazine are respectively added with the Ma Shi reagent, the 1- (2-fluorophenyl) piperazine is colorless, and the 1- (4-methylphenyl) piperazine is light yellow, both show a single color and can be distinguished.

7. The method for color development test of piperazine novel mental active substances according to claim 3, wherein the establishment of the color development test screening program diagram further comprises the following steps of, on the basis of the identification of 9 piperazines by adding siemens reagent solution A and solution B, continuously adding a color developing agent to piperazine which does not show a single color, and performing identification, wherein the method comprises the following steps:

respectively adding the nitric acid-sulfuric acid reagent into non-colored 4,4 '-difluorobenzylpiperazine, 1- (2, 3-dichlorophenyl) piperazine and p-chlorophenyl piperazine, wherein the 4,4' -difluorobenzylpiperazine is light yellow, the 1- (2, 3-dichlorophenyl) piperazine is colorless, and the p-chlorophenyl piperazine is brownish red, and both show a single color and can be distinguished.

8. The method for color development test of piperazine novel mental active substances according to claim 3, wherein the establishment of the color development test screening program diagram further comprises the following steps of, on the basis of the identification of 9 piperazines by adding siemens reagent solution A and solution B, continuously adding a color developing agent to piperazine which does not show a single color, and performing identification, wherein the method comprises the following steps:

the non-developed 1- (3-chlorphenyl) piperazine and 1- (4-methoxyphenyl) piperazine are respectively added with the Ma-Shi reagent, wherein the 1- (3-chlorphenyl) piperazine is yellow, and the 1- (4-methoxyphenyl) piperazine is colorless, and both show a single color and can be distinguished.

9. The method for color development testing of piperazine novel psychoactive substances according to any one of claims 1 to 8, wherein when a color development testing screening program diagram is established, the reaction color is recorded in characters, the color of each color development reaction substance is recorded by photographing, and the color image recorded by photographing is inserted into a position corresponding to the color development testing screening program diagram.

Technical Field

The invention relates to the field of identification of new psychoactive substances, in particular to a detection method which can meet the field rapid detection requirement of 22 piperazine new psychoactive substances by only 4 chromogenic reagents (a Siemens reagent, a vanillin reagent, a Ma-Chi reagent and a nitric acid-sulfuric acid reagent).

Background

New Psychoactive Substances (NPS), also known as pro-drugs, are drug analogs or derivatives formed by modifying or altering the molecular structure of controlled drugs in order to circumvent existing drug control measures. The properties of the novel psychoactive substance are similar to those of the conventional drugs, have drug dependence and hallucinogenic effect, can lead people to addiction, but are not regulated by the law for the most part. The abuse for a long time can cause damage to the health of human bodies, and various diseases and even death are caused; more seriously, abuse of new psychoactive substances can cause the illusion of the smoker, and further violently attack the smoker or other people to trigger various violent and terroristic events.

The piperazine new mental active substances are used as a class of NPS with the most serious hazard situation, only a few kinds of NPS are listed and controlled in China, and after the first piperazine new mental active substance, namely benzylpiperazine, is listed and managed in a category of mental drugs and narcotics in 2013, three kinds of piperazine new mental active substances, namely 1, 4-dibenzylpiperazine, 1- (3-chlorophenyl) piperazine and 1- (3-trifluoromethylphenyl) piperazine, are added in a category of non-medicinal narcotics and controlled varieties of mental drugs in 2015, so far, a large amount of piperazine new mental active substances are not listed and managed in China, a supervision blank exists, and great harm is caused to the society.

At present, mature industrial standards and analytical methods are established for common controlled drugs such as methamphetamine, heroin, ketamine and the like in China, mature rapid detection technologies such as an appearance inspection method and a color development inspection method are also provided for the common controlled drugs, wherein the color development inspection method is established on the basis that different colors are presented by using chemical components of the drugs and reacting without reagents, and relatively speaking, the accuracy and the directivity are high. However, for the emerging new piperazine psychoactive substances, no accurate, effective and rapid on-site detection means exists in China at present, the traditional laboratory detection method is complicated in operation steps and long in identification period, time is consumed in drug detection cases, and the problem of missing a battle plane is avoided. Therefore, the research and the popularization of the on-site rapid detection method of the piperazine novel mental active substances with simplicity, convenience for implementation and higher timeliness have very important significance for detecting the drug-related cases.

Disclosure of Invention

The invention aims to solve the problems that no accurate, effective and rapid on-site detection means exists in China for emerging new piperazine mental active substances, the traditional laboratory detection method has complicated operation steps and long identification period, consumes more time in drug detection cases and is difficult to avoid missing a battle opportunity. Therefore, the invention provides a color development examination screening program for 22 kinds of piperazine new psychoactive substances by adopting 4 kinds of conventional drug color development reagents (Siemens reagent, vanillin reagent, Ma Shi reagent and nitric acid-sulfuric acid reagent), and a set of examination method capable of meeting the field rapid examination requirement of the piperazine new psychoactive substances is obtained. The technical scheme of the invention is as follows.

A color development test method for piperazine novel psychoactive substances uses 4 color development reagents to carry out color development reaction on known piperazine novel psychoactive substances, establishes a color development test screening program diagram, and then carries out species identification on the piperazine novel psychoactive substances of unknown species to be detected by using the 4 color development reagents according to the color development test screening program diagram.

The known classes of novel psychoactive substances of the piperazine family include N-benzylpiperazine, 1-benzyl-4-methylpiperazine hydrochloride, 1, 4-dibenzylpiperazine, 1- (3-chlorobenzyl) piperazine, 4' -difluorobenzylpiperazine, 1-piperonylpiperazine, 1- (3-trifluoromethylphenyl) piperazine, 1- (4-fluorophenyl) piperazine, 1- (2, 4-difluorophenyl) piperazine, 1- (2-fluorophenyl) piperazine, 1- (3-chlorophenyl) piperazine, 1- (2, 3-dichlorophenyl) piperazine, 1- (3, 4-dichlorophenyl) piperazine, p-chlorophenylpiperazine, 1- (3-chlorophenyl) -4- (3-chloropropyl) piperazine, N-benzylpiperazine, 1, 4-chlorobenzylpiperazine, 1- (3-chlorobenzyl) piperazine, N-benzylpiperazine, 1- (3-chlorobenzyl) piperazine, N-methyl-piperazine, N-methyl-, 1- (2-methoxyphenyl) piperazine, 1- (3-methoxyphenyl) piperazine, 1- (4-methoxyphenyl) piperazine, 1- (2-methylphenyl) piperazine, 1- (3-methylphenyl) piperazine, 1- (4-methylphenyl) piperazine, and N-phenylpiperazine.

The chromogenic reagents used include siemens reagent, vanillin reagent, mardomo reagent and nitric-sulfuric acid reagent.

The step of testing the piperazine-based novel psychoactive substances of unknown species comprises: firstly, the Siemens reagent is respectively added into each piperazine new psychoactive substance of unknown species, and if the Siemens reagent corresponds to a single color recorded in a color development test screening program diagram, the species of the piperazine new psychoactive substance can be directly judged; and if the color is corresponding to the repeated color recorded in the color development examination screening program diagram, taking the new piperazine psychoactive substances which are not subjected to color development according to the repeated color, adding the vanillin reagent, the majiki reagent or the nitric acid-sulfuric acid reagent according to the color development examination screening program diagram, and sequentially identifying the type of each piperazine substance according to the color development result.

Further, the Siemens reagent comprises two components of solution A and solution B, and is prepared by the following steps: dissolving 0.9g of sodium nitrosyl ferricyanide in 50-130 mL of water, adding 5-15 mL of acetaldehyde, and shaking up; and B, liquid B: 2g of sodium carbonate is dissolved in 50-150 mL of water and shaken up.

The vanillin reagent comprises three components of solution A, solution B and solution C, and is prepared by the following steps: dissolving 0.4g of vanillin in 15-25 mL of 95% ethanol, adding 0.1-1 mL of acetaldehyde, and shaking up; and B, liquid B: hydrochloric acid; and C, liquid C: chloroform.

The maxjestic reagent comprises a component and is prepared by the following steps of adding 10mL of 98% concentrated sulfuric acid into 0.5-1.5 mL of formaldehyde solution and shaking up.

The nitric acid-sulfuric acid reagent comprises a component and is prepared by the following steps of adding 0.1-1 mL of nitric acid into 10mL of 98% concentrated sulfuric acid and shaking up.

Further, the establishment of the chromogenic examination screening program diagram comprises the following steps:

(1) the siemens reagent solution a was added to 22 kinds of the known piperazine new psychoactive substances: n-phenylpiperazine, 1-benzyl-4-methylpiperazine hydrochloride, 1- (4-fluorophenyl) piperazine, 1- (3-methoxyphenyl) piperazine, N-phenylpiperazine, 1- (2, 4-difluorophenyl) piperazine, 1- (3-chlorobenzyl) piperazine, 1- (2-methylphenyl) piperazine, and 1- (3-methylphenyl) piperazine all appeared blue; the 1- (2-fluorophenyl) piperazine, the 1- (4-methylphenyl) piperazine, the 4,4' -difluorobenzylpiperazine, the 1- (2, 3-dichlorophenyl) piperazine, the p-chlorophenyl piperazine, the 1- (3-chlorophenyl) piperazine, the 1- (4-methoxyphenyl) piperazine, the 1- (3, 4-dichlorophenyl) piperazine and the 1- (3-chlorophenyl) -4- (3-chloropropyl) piperazine all show green; ③ 1- (2-methoxyphenyl) piperazine and 1-piperonyl piperazine show dark blue; the 1, 4-dibenzyl piperazine shows light yellow; fifthly, the 1- (3-trifluoromethyl phenyl) piperazine shows brown yellow;

(2) then, adding a Siemens reagent solution A into each known piperazine novel mental active substance, and then adding a Siemens reagent solution B: n-phenylpiperazine, 1-benzyl-4-methylpiperazine hydrochloride, 1- (4-fluorophenyl) piperazine and 1- (3-methoxyphenyl) piperazine are blue; the N-phenylpiperazine is green, and is brownish red after fully reacting for 1-3 min; the 1- (2, 4-difluorophenyl) piperazine is purple, and is brown after fully reacting for 1-3 min; 1- (3-chlorobenzyl) piperazine exhibits a dark blue color; 1- (2-methylphenyl) piperazine and 1- (3-methylphenyl) piperazine are purple and are purple after fully reacting for 1-3 min; ② 1- (2-fluorophenyl) piperazine and 1- (4-methylphenyl) piperazine are purple, and are purple red after fully reacting for 1-3 min; 4,4' -difluorobenzylpiperazine, 1- (2, 3-dichlorophenyl) piperazine and p-chlorophenyl piperazine are blue; 1- (3-chlorophenyl) piperazine and 1- (4-methoxyphenyl) piperazine are brown; 1- (3, 4-dichlorophenyl) piperazine presents brown yellow; 1- (3-chlorophenyl) -4- (3-chloropropyl) piperazine appears yellow; ③ 1- (2-methoxyphenyl) piperazine and 1-piperonyl piperazine show dark blue, after fully reacting for 1-3 min, the 1- (2-methoxyphenyl) piperazine still shows dark blue, and the 1-piperonyl piperazine shows purple; fourthly, enabling the 1, 4-dibenzyl piperazine to be light yellow and to be brown after fully reacting for 1-3 min; fifthly, the 1- (3-trifluoromethyl phenyl) piperazine is brownish yellow;

(3) according to the above process of adding the Siemens reagent A liquid and B liquid, N-phenylpiperazine, 1- (2, 4-difluorophenyl) piperazine, 1- (3-chlorobenzyl) piperazine, 1- (3, 4-dichlorophenyl) piperazine, 1- (3-chlorophenyl) -4- (3-chloropropyl) piperazine, 1- (2-methoxyphenyl) piperazine, 1-piperonyl piperazine, 1, 4-dibenzyl piperazine, and 1- (3-trifluoromethylphenyl) piperazine were added, and these 9 piperazines showed a single color and could be directly distinguished.

Further, the establishment of the color development examination screening program diagram further comprises the following steps of continuously adding the color developing agent to piperazine which does not show a single color for identification on the basis of identifying 9 types of piperazine by adding the Siemens reagent A liquid and the Siemens reagent B liquid, wherein the steps comprise:

(4) taking un-developed (i) N-phenylpiperazine, 1-benzyl-4-methylpiperazine hydrochloride, 1- (4-fluorophenyl) piperazine and 1- (3-methoxyphenyl) piperazine, respectively and continuously adding the vanillin reagent A liquid, the B liquid and the C liquid, and fully reacting for 1-3 min to obtain colorless N-phenylpiperazine and 1-benzyl-4-methylpiperazine hydrochloride, light yellow 1- (4-fluorophenyl) piperazine and pink 1- (3-methoxyphenyl) piperazine;

1- (4-fluorophenyl) piperazine and 1- (3-methoxyphenyl) piperazine show a single color and can be directly distinguished;

(5) respectively adding the nitric acid-sulfuric acid reagent into the un-colored (i) N-phenylpiperazine and 1-benzyl-4-methylpiperazine hydrochloride, wherein the N-phenylpiperazine is light yellow, and the 1-benzyl-4-methylpiperazine hydrochloride is colorless;

the N-phenylpiperazine and the 1-benzyl-4-methylpiperazine hydrochloride show a single color and can be directly distinguished.

Further, the establishment of the color development examination screening program diagram further comprises the following steps of continuously adding the color developing agent to piperazine which does not show a single color for identification on the basis of identifying 9 types of piperazine by adding the Siemens reagent A liquid and the Siemens reagent B liquid, wherein the steps comprise:

(6) taking un-developed 1- (2-methylphenyl) piperazine and 1- (3-methylphenyl) piperazine, respectively and continuously adding the vanillin reagent A liquid, B liquid and C liquid, and reacting for 1-3 min, wherein the 1- (2-methylphenyl) piperazine is colorless, and the 1- (3-methylphenyl) piperazine is pink; 1- (2-methylphenyl) piperazine and 1- (3-methylphenyl) piperazine respectively have a single color and can be directly distinguished.

Further, the establishment of the color development examination screening program diagram further comprises the following steps of continuously adding the color developing agent to piperazine which does not show a single color for identification on the basis of identifying 9 types of piperazine by adding the Siemens reagent A liquid and the Siemens reagent B liquid, wherein the steps comprise:

(7) the non-developed 1- (2-fluorophenyl) piperazine and 1- (4-methylphenyl) piperazine are respectively added with the Ma Shi reagent, the 1- (2-fluorophenyl) piperazine is colorless, and the 1- (4-methylphenyl) piperazine is light yellow, both show a single color and can be distinguished.

Further, the establishment of the color development examination screening program diagram further comprises the following steps of continuously adding the color developing agent to piperazine which does not show a single color for identification on the basis of identifying 9 types of piperazine by adding the Siemens reagent A liquid and the Siemens reagent B liquid, wherein the steps comprise:

(8) respectively adding the nitric acid-sulfuric acid reagent into non-colored 4,4 '-difluorobenzylpiperazine, 1- (2, 3-dichlorophenyl) piperazine and p-chlorophenyl piperazine, wherein the 4,4' -difluorobenzylpiperazine is light yellow, the 1- (2, 3-dichlorophenyl) piperazine is colorless, and the p-chlorophenyl piperazine is brownish red, and both show a single color and can be distinguished.

Further, the establishment of the color development examination screening program diagram further comprises the following steps of continuously adding the color developing agent to piperazine which does not show a single color for identification on the basis of identifying 9 types of piperazine by adding the Siemens reagent A liquid and the Siemens reagent B liquid, wherein the steps comprise:

(9) the non-developed 1- (3-chlorphenyl) piperazine and 1- (4-methoxyphenyl) piperazine are respectively added with the Ma-Shi reagent, wherein the 1- (3-chlorphenyl) piperazine is yellow, and the 1- (4-methoxyphenyl) piperazine is colorless, and both show a single color and can be distinguished.

Further, when the color development examination screening program diagram is established, the reaction colors are recorded in a text mode, the color of each substance in the color development reaction is recorded in a picture mode, and the color picture recorded in the picture mode is inserted into the corresponding position of the color development examination screening program diagram.

The invention has the beneficial effects that:

the invention establishes a color development test method for 22 piperazine new psychoactive substances, which is simple, convenient and quick, uses few reagents, does not need large-scale equipment and complex instruments, only needs to prepare 4 color development reagents (Siemens reagent, vanillin reagent, makory reagent and nitric acid-sulfuric acid reagent) in advance, utilizes a test tube, a beaker, a glass slide, a white porcelain plate or a thick white cardboard to carry out on-site quick test on the 22 piperazine new psychoactive substances, is beneficial to criminal detection technical inspectors to primarily screen suspicious piperazine new psychoactive substances on site, can shorten the period of drug test, enhance the pertinence of drug test, prevent the transfer and loss of the piperazine new psychoactive substances, and effectively hit lawless molecules.

Drawings

FIG. 1 is a diagram showing the reaction operation of the piperazine-based novel psychoactive substance provided in example 1 with a coloring agent.

FIG. 2 is a diagram showing the effect of the chromogenic test screening program provided in example 1.

FIG. 3 is a histogram of color development abundance of various color developing reagents provided in combination with example 1 and comparative example 1.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention clearer, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. The examples, in which specific conditions are not specified, were conducted under conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used are not indicated by the manufacturer, and are all conventional products available commercially.