阅读说明：本技术 一种近红外二区共径离轴光学-ct双模态成像系统及方法 (Near-infrared two-region co-radial off-axis optical-CT dual-mode imaging system and method ) 是由 郭红波 赵竟雯 贺小伟 宋小磊 侯榆青 易黄建 赵凤军 任玉丹 刘艳秋 赵恒娜 于 2020-07-24 设计创作，主要内容包括：本发明公开了一种近红外二区共径离轴光学-CT双模态成像系统和方法,包括：共径激发模块、信号分离模块、光信号采集模块、CT图像采集模块和数据处理模块。本发明将近红外二区荧光成像与CT成像巧妙耦合在同一系统,近红外二区成像技术可以突破光学信号穿透深度,更好的实现肿瘤的早期检测,结合CT成像可以提供待检测生物体结构信息的优点,可以减小多模态成像在时域和空间中带来的扰动误差,同时获得白光数据,荧光数据,CT投影数据,降低实验难度,减小数据配准出现的误差。(The invention discloses a near-infrared two-region co-radial off-axis optical-CT dual-mode imaging system and a method, which comprises the following steps: the device comprises a common-path excitation module, a signal separation module, an optical signal acquisition module, a CT image acquisition module and a data processing module. The near-infrared two-region fluorescence imaging and the CT imaging are skillfully coupled in the same system, the near-infrared two-region imaging technology can break through the penetration depth of optical signals, the early detection of tumors can be better realized, the advantage that the CT imaging can provide the structural information of the organism to be detected is combined, the disturbance error caused by multi-mode imaging in time domain and space can be reduced, simultaneously white light data, fluorescence data and CT projection data are obtained, the experiment difficulty is reduced, and the error caused by data registration is reduced.)

1. A near-infrared two-region co-radial off-axis optical-CT dual-mode imaging system is characterized by comprising: a co-path excitation module (1), a signal separation module (2), an optical signal acquisition module (3), a CT image acquisition module (4) and a data processing module (5), wherein,

the co-path excitation module (1) is used for forming mixed light beams by white light and laser and irradiating the mixed light beams on the organism to be detected;

the signal separation module (2) is used for separating the mixed light beam passing through the organism to be detected into a white light signal and a near-infrared two-region fluorescence signal;

the optical signal acquisition module (3) is used for respectively acquiring the white light signal and the near-infrared two-region fluorescence signal obtained by the signal separation module (2);

the CT image acquisition module (4) is used for acquiring projection data of an organism to be detected;

the data processing module (5) is used for reconstructing the projection data obtained by the CT image acquisition module (4) in three dimensions to obtain a three-dimensional CT image of the organism to be detected, and registering the white light signal data and the near-infrared two-region fluorescence signal data obtained by the optical signal acquisition module (3) on the three-dimensional CT image.

2. The near-infrared two-zone co-radial off-axis optical-CT dual-mode imaging system according to claim 1, wherein the co-radial excitation module (1) comprises a white light source (11), a laser (12), a double mirror (13) and two sets of beam expanders (14), the white light source (11) and one set of beam expanders (14) are disposed on one side of the double mirror (13), and the laser (12) and the other set of beam expanders (14) are disposed on the other side of the double mirror (13).

3. The near-infrared two-zone co-radial off-axis optical-CT dual-modality imaging system according to claim 1, wherein the signal separation module (2) comprises a wedge-shaped beam splitter.

4. The near-infrared two-zone co-radial off-axis optical-CT dual-mode imaging system as claimed in claim 1, wherein the optical signal collection module (3) comprises two sets of optical filters (31), two sets of relay lens groups (32), a white light signal collection camera (33) and a near-infrared two-zone fluorescence collection camera (34), the white light signal collection camera (33), the set of optical filters (31) and the set of relay lens groups (32) are arranged on one side of the signal separation module (2), and the near-infrared two-zone fluorescence collection camera (34), the other set of optical filters (31) and the other set of relay lens groups (32) are arranged on the other side of the signal separation module (2).

5. The near-infrared two-region co-radial off-axis optical-CT dual-mode imaging system according to claim 1, wherein the CT image acquisition module (4) comprises a radiation source (41), a stage (42) for placing the organism to be detected and a detection plate (43), the CT image acquisition module (4) is disposed between the co-radial excitation module (1) and the signal separation module (2), and the stage (42) can provide different directional degrees of freedom for the organism to be detected.

6. The near-infrared two-region co-radial off-axis optical-CT dual-modality imaging system according to claim 1, wherein the data registration in the data processing module (5) comprises: firstly, registering white light signal data obtained by a light signal acquisition module (3) on a three-dimensional CT image to obtain an initial image; and then registering the near-infrared two-region fluorescence signal data obtained by the optical signal acquisition module (3) to the initial image.

7. The near-infrared two-region co-radial off-axis optical-CT dual-modality imaging system according to claim 1, wherein the data processing module (5) further comprises a denoising module for removing background noise of white light signals and near-infrared two-region fluorescence signals and a dead pixel compensation module for removing dead pixels of CT projection data.

8. The near-infrared two-zone co-radial off-axis optical-CT dual-modality imaging system of claim 1, further comprising a system control module for controlling the activation and deactivation of the radiation source in the co-radial excitation module and the activation, preheating and parameter setting of each device in the optical signal acquisition module.

9. The near-infrared two-zone co-radial off-axis optical-CT dual-modality imaging system of claim 1, further comprising a power transmission module, wherein the power transmission module comprises a rotating table for driving the self-rotation of the organism to be detected and a translation table for moving each module.

10. A near-infrared two-region co-radial off-axis optical-CT dual-mode imaging method is characterized in that the imaging system of any one of claims 1 to 9 is adopted for imaging, and the method specifically comprises the following steps:

illuminating an organism to be detected by adopting a white light source, starting the laser light source, mixing the laser beam and the white light beam in a common path to form a mixed light beam, irradiating the mixed light beam on the organism to be detected, performing signal separation on the mixed light beam passing through the organism to be detected, and collecting separated signals;

starting CT to scan the organism to be detected to obtain a three-dimensional CT image;

and registering the separated signal with the three-dimensional CT image to obtain a registered image.

Technical Field

The invention belongs to the technical field of fluorescence imaging, and relates to a near-infrared two-region co-radial off-axis optical-CT dual-mode imaging system and method.

Background

At present, the molecular imaging technology is well applied to tumor detection and surgical navigation, and various methods for detecting and imaging tumors exist in the industry, and the imaging precision is mature. For example: fluorescence tomography, X-ray imaging, magnetic resonance imaging, ultrasound, etc., which can be used to detect and diagnose human tumors. The wavelength of the traditional optical imaging is mostly concentrated in a wavelength band from visible light to near infrared, and because biological tissues have strong absorption and scattering in the wavelength band, the signal-to-noise ratio and the tissue penetration depth are both low. Compared with the visible light and the light emitted in the near-infrared region I, the light emitted in the near-infrared region II (NIR-II, 1000-1700nm) has longer wavelength, lower light absorption and auto-fluorescence, and the scattering loss is reduced by about 1000 times, so that the penetration depth of the light in the tissue and the spatial resolution and sensitivity of imaging can be remarkably improved, and the method is considered to be a next-generation advanced optical imaging method. On the other hand, the important application value of the CT technology in medical imaging and the characteristics of the molecular imaging technology are important to understand the information of the living body from different angles, and the multimodality information is fused into a research hotspot.

Because biological information acquired by single-mode imaging has limitations, multiple modes are required to acquire data of different layers, and then information fusion is performed to realize complementation among the various modes, the existing multi-mode imaging comprises the following steps: based on different position spaces, the sample position moves in different experimental processes, so that image registration is influenced, and errors are brought to experimental results; data acquisition based on different time domains needs a lot of experiments, so that the experimental process is more complicated, the experimental operation difficulty is increased, and the experimental error probability is increased.

Disclosure of Invention

In order to solve the defects in the prior art, the invention provides a near-infrared two-region co-radial off-axis optical-CT dual-mode imaging system and method, which solve the problem of large disturbance error in time domain and space caused by the existing multi-mode imaging technology.

In order to solve the technical problems, the invention adopts the following technical scheme:

a near-infrared two-region co-radial off-axis optical-CT dual-mode imaging system comprises: a co-path excitation module, a signal separation module, an optical signal acquisition module, a CT image acquisition module and a data processing module, wherein,

the co-path excitation module is used for forming mixed light beams by the white light and the laser and irradiating the mixed light beams on the organism to be detected;

the signal separation module is used for separating the mixed light beam passing through the organism to be detected into a white light signal and a near-infrared two-region fluorescence signal;

the optical signal acquisition module is used for respectively acquiring the white light signal and the near-infrared two-region fluorescence signal obtained by the signal separation module;

the CT image acquisition module is used for acquiring projection data of an organism to be detected;

the data processing module is used for reconstructing projection data obtained by the CT image acquisition module in a three-dimensional mode to obtain a three-dimensional CT image of the organism to be detected, registering white light signal data obtained by the optical signal acquisition module on the three-dimensional CT image to obtain an initial image, and registering near-infrared two-region fluorescence signal data obtained by the optical signal acquisition module on the initial image.

Specifically, the common-path excitation module comprises a white light source, a laser, a double-sided mirror and two groups of beam expanders, wherein the white light source and one group of beam expanders are arranged on one side of the double-sided mirror, and the laser and the other group of beam expanders are arranged on the other side of the double-sided mirror.

Specifically, the signal separation module comprises a wedge-shaped beam splitter.

Specifically, the optical signal acquisition module comprises two groups of optical filters, two groups of relay lens groups, a white light signal acquisition camera and a near-infrared two-zone fluorescence acquisition camera, the white light signal acquisition camera, the optical filters and the relay lens groups are arranged on one side of the signal separation module, and the near-infrared two-zone fluorescence acquisition camera, the optical filters and the relay lens groups are arranged on the other side of the signal separation module.

Specifically, the CT image acquisition module comprises a ray source, an object stage for placing an organism to be detected and a detection plate, the CT image acquisition module is arranged between the common-path excitation module and the signal separation module, and the object stage can provide freedom degrees in different directions for the organism to be detected.

Specifically, the data registration in the data processing module includes: firstly, registering white light signal data obtained by a light signal acquisition module on a three-dimensional CT image to obtain an initial image; and registering the near-infrared two-region fluorescence signal data obtained by the optical signal acquisition module to the initial image.

Furthermore, the data processing module further comprises a denoising module and a dead pixel compensation module, wherein the denoising module is used for removing background noise of the white light signal and the near-infrared two-region fluorescence signal, and the dead pixel compensation module is used for removing dead pixels of the CT projection data.

Furthermore, the system also comprises a system control module which is used for controlling the starting and the closing of the ray source in the co-path excitation module and the starting, the preheating and the parameter setting of each device in the optical signal acquisition module.

Furthermore, the system also comprises a transmission power module, wherein the transmission power module comprises a rotating table used for driving the organism to be detected to rotate automatically and a translation table used for driving each module to move.

The invention also discloses a near-infrared two-region co-radial off-axis optical-CT bimodal imaging method, which adopts the imaging system to perform imaging and specifically comprises the following steps:

illuminating an organism to be detected by adopting a white light source, starting the laser light source, mixing the laser beam and the white light beam in a common path to form a mixed light beam, irradiating the mixed light beam on the organism to be detected, performing signal separation on the mixed light beam passing through the organism to be detected, and collecting separated signals;

starting CT to scan the organism to be detected to obtain a three-dimensional CT image;

and registering the separated signal with the three-dimensional CT image to obtain a registered image.

Compared with the prior art, the invention has the beneficial effects that:

(1) the near-infrared two-region fluorescence imaging and the CT imaging are skillfully coupled in the same system, the near-infrared two-region imaging technology can break through the penetration depth of optical signals, the early detection of tumors can be better realized, the advantage that the CT imaging can provide the structural information of the organism to be detected is combined, the disturbance error caused by multi-mode imaging in time domain and space can be reduced, simultaneously white light data, fluorescence data and CT projection data are obtained, the experiment difficulty is reduced, and the error caused by data registration is reduced.

(2) The invention adopts a common-path excitation mode to couple the white light beam with the laser beam light path, thereby ensuring that the excitation light beam and the illumination light beam are simultaneously projected on a straight line and ensuring that the white light signal and the fluorescence signal in the optical acquisition module are simultaneously acquired.

(3) The system has simple light path structure and simple operation, and effectively provides convenience for subsequent image fusion.

Drawings

FIG. 1 is a block diagram of a dual-modality imaging system according to an embodiment of the present invention.

Fig. 2 is a flow chart of a bimodal imaging method according to an embodiment of the present invention.

The meaning of the various reference numbers in the figures:

1-a co-path excitation module, 2-a signal separation module, 3-an optical signal acquisition module, 4-a CT image acquisition module and 5-a data processing module;

11-white light source, 12-laser, 13-double mirror, 14-beam expanding mirror;

31-an optical filter, 32-a relay lens group, 33-a white light signal collecting camera and 34-a near infrared two-zone fluorescence collecting camera;

41-ray source, 42-object stage and 43-detection plate.

Detailed Description

The invention relates to a coaxial off-axis device, which is used for coaxial excitation and off-axis collection, namely, white light rays and laser rays form mixed beams to irradiate an organism to be detected, a fluorescence signal is excited, and the excited signal is separated and collected.

The following embodiments of the present invention are provided, and it should be noted that the present invention is not limited to the following embodiments, and all equivalent changes based on the technical solutions of the present invention are within the protection scope of the present invention.