阅读说明：本技术 一种用于生物体的可调进针深度辅助电极植入系统及电极植入方法 (Auxiliary electrode implantation system and electrode implantation method capable of adjusting needle insertion depth for organism ) 是由 王浩 王绍康 吴锦 方轲 余晨 于 2021-06-25 设计创作，主要内容包括：本发明公开了一种用于生物体的可调进针深度辅助电极植入系统及电极植入方法,涉及科学实验仪器技术领域。提出了一种可解决现有技术尖锐度不足、无可视化数据、精度不足以及辅助装置无法撤离等问题的用于生物体的可调进针深度辅助电极植入系统及电极植入方法。包括植入端、推进模块、电极夹持模块以及壳体；所述植入端包括针栓和针头,所述针头的尾端与针栓可拆卸的相连接；所述推进模块包括与壳体相连接的第一级推进装置、第二级推进装置,通过所述电极夹持模块夹持电极丝。以实现对电极植入深度的数据化、可视化,有利于对具有良好实验效果的肌肉电极植入位点的复现。实现尖锐植入和较短路径植入,造成的组织损伤较小。(The invention discloses an auxiliary electrode implantation system and an auxiliary electrode implantation method for an organism with adjustable needle insertion depth, and relates to the technical field of scientific experimental instruments. The auxiliary electrode implantation system and the electrode implantation method capable of adjusting the needle insertion depth for the organism can solve the problems that the sharpness is insufficient, visual data is not available, the precision is insufficient, an auxiliary device cannot be evacuated and the like in the prior art. Comprises an implanting end, a pushing module, an electrode clamping module and a shell; the implanting end comprises a pintle and a needle head, and the tail end of the needle head is detachably connected with the pintle; the propelling module comprises a first-stage propelling device and a second-stage propelling device which are connected with the shell, and the electrode wire is clamped through the electrode clamping module. The method realizes the datamation and visualization of the electrode implantation depth, and is favorable for the reproduction of the muscle electrode implantation sites with good experimental effect. The sharp implantation and the short path implantation are realized, and the damage to the tissue is less.)

1. An adjustable needle insertion depth auxiliary electrode implantation system for a living body, which is characterized by comprising an implantation end, an advancing module, an electrode clamping module and a shell (4);

the implanting end comprises a pintle (11) and a needle head (12), and the tail end of the needle head (12) is detachably connected with the pintle (11);

the propelling module comprises a first-stage propelling device and a second-stage propelling device which are connected with the shell (4), and the pintle (11) is connected to the first-stage propelling device and is driven by the first-stage propelling device to do linear reciprocating motion relative to the shell (4); the electrode clamping module is connected to the second-stage propelling device and driven by the second-stage propelling device to do linear reciprocating motion relative to the shell (4), and the electrode wire is clamped through the electrode clamping module.

2. The adjustable needle insertion depth auxiliary electrode implantation system for living organisms according to claim 1, wherein the housing (4) is provided with a primary scale (41) for representing the displacement amount of the primary propelling device and a secondary scale (42) for representing the displacement amount of the secondary propelling device.

3. The adjustable needle insertion depth auxiliary electrode implantation system for living organisms according to claim 1, wherein an axial through hole (120) for accommodating an electrode wire is formed in the needle head (12).

4. The adjustable needle depth auxiliary electrode implantation system for living organisms according to claim 1, 2 or 3, wherein the first stage advancing device comprises a threaded tube (21), a hollow screw (22), a gear driving mechanism (23) and a driving handle (24);

one end of the hollow screw rod (22) is hinged in the shell (4), the threaded pipe (21) is sleeved with the hollow screw rod (22) and is in threaded connection with the hollow screw rod (22), a limiting sliding block (211) is fixedly connected to the outer wall of the threaded pipe (21), a long-strip-shaped sliding hole (212) parallel to the threaded pipe (21) is formed in the shell (4), and the limiting sliding block (211) is connected in the long-strip-shaped sliding hole (212) in a sliding mode;

the gear driving mechanism (23) comprises a driving gear and a driven gear which are mutually meshed, the driving gear is hinged in the shell, the driven gear is fixedly connected with the hollow screw (22), and the driven gear and the hollow screw (22) are coaxial; the driving handle (24) is fixedly connected with the driving gear;

the pintle (11) is fixedly connected to the hollow screw (22), and the primary scale (41) is located on one side of the strip-shaped sliding hole (212).

5. The auxiliary electrode implantation system with the adjustable needle insertion depth for the organism as claimed in claim 1, 2 or 3, wherein the second stage propulsion device comprises a bolt (25) and a sleeve (26), one side of the housing (4) away from the needle head (22) is provided with a connecting part connected with the bolt into a whole, the connecting part is provided with a threaded blind hole matched with the bolt (25), and the bottom of the threaded blind hole is provided with a thread insertion channel communicated with the central part of the hollow screw rod (22);

the bolt (25) is hollow and is connected in the threaded blind hole through threads, and the electrode clamping module is connected to one side, far away from the connecting part, of the bolt (25);

the sleeve (26) is sleeved on the connecting part, the sleeve (26) and the head of the bolt (25) are connected into a whole, and the secondary scale (42) is positioned on the outer wall of the connecting part.

6. The adjustable needle insertion depth auxiliary electrode implantation system for living organisms according to claim 5, wherein the electrode clamping module comprises a hollow stud (31), a three-petal clamp (32) and a fastener (33) with internal threads;

hollow stud (31) fixed connection is on bolt (25) the terminal surface of connecting portion dorsad, three lamella press from both sides (32) fixed connection is on hollow stud (31), fastener (33) through threaded connection on hollow stud (31) and fastener (33) open be equipped with three lamella press from both sides the bell jar of (32) adaptation.

7. The adjustable needle insertion depth auxiliary electrode implantation system for living organisms according to claim 6, wherein the three-petal clamp (32), the hollow stud (31), the bolt (25), the connecting part, the hollow screw (22), the threaded tube (21) and the needle (12) are all kept coaxial.

8. An electrode implantation method of the adjustable needle insertion depth auxiliary electrode implantation system for living organisms according to claim 1, wherein the electrode implantation is performed according to the following steps:

s1, primary installation;

s1.1, fixing an implementation object, and determining an initial implantation position and a needle type of an electrode;

s1.2, installing an electrode wire in the auxiliary electrode implantation system with the adjustable needle insertion depth;

s1.3, recording the position of the limiting slide block in the primary scale when the needle is completely retracted, and recording as an initial position;

s1.4, calculating the displacement of the needle head according to the initial implantation position of the electrode and the type of the needle head;

s1.5, on the basis of the initial position, combining the displacement calculated in the step S1.4, and driving the needle head to extend out towards one side far away from the shell through the first-stage propelling device, so that the displacement of the limiting slide block reaches the displacement calculated in the step S1.4;

s1.6, zeroing the initial position of the second-stage propelling device;

s1.7, sequentially passing the electrode wire through the three-petal clamp, the first-stage propelling device, the second-stage propelling device and the needle head, and finally keeping the electrode wire flush with the needle tip of the needle head; finally, the fastener is rotated to lock the three-petal clamp;

s2, primary electrode implantation: starting electrode implantation, completely implanting the needle into a living body, and controlling the needle to retract 1-2mm by the first-stage propulsion device, so that the electrode wire is exposed in the experimental object;

s2, testing: testing whether the experimental effect of the current implantation point is good; if yes, go to step S4, otherwise go to step S3;

s3, modifying the depth;

s3.1, keeping the shell still, and adjusting the position of the needle head through the first-stage propelling device;

s3.2, driving the electrode wire to be flush with the needle tip of the needle head through a second-stage propelling device;

s3.3, controlling the needle to retract by 1-2mm through the first-stage propelling device, so that the electrode wire is exposed in the experimental object;

s3.4, returning to the step S2;

s4, withdrawing the needle;

s4.1, controlling the needle to retract outside the experimental object through the first-stage propelling device;

s4.2, the electrode wire exposed out of the skin surface is kept fixed with the experimental object;

s4.3, rotating the fastening piece to loosen the locking of the three-petal clamp;

s4.4, removing the auxiliary electrode implantation system with the adjustable needle insertion depth;

s5, start experiment: and opening external equipment connected with the wire electrode to start the experiment.

Technical Field

The invention relates to the technical field of scientific experimental instruments, in particular to an auxiliary electrode implantation system with adjustable needle insertion depth for organisms and a control method.

Background

In the field of biological research, invasive electrode implantation techniques are widely used, and particularly, the implantation of electrodes is a very important step in the study of physiological electrical signals generated by various activities of animals, such as: the implantation position and the implantation depth of the electrode have a great influence on the final experimental result. In the electrode implantation process, the tissue damage caused by an implantation mode with a sharp implantation end, a high implantation speed and a short path is small, and for the measurement of the electromyographic signals, the electrode which is commonly used is a wire electrode, the rigidity of the electrode is not enough, and an external auxiliary implantation tool is needed to lead the electrode to be implanted into the muscle tissue.

In recent years, researchers have proposed various perfection schemes for the system, such as: the chinese patent application with publication number CN112245802A provides a guide wire handle and an implantable medical system, which is to fixedly connect a handle at the end of the guide wire with a hollow electrode, i.e. the electrode wraps the guide wire at the outside, and the electrode straightness is supported and ensured by the rigidity of the guide wire, thereby assisting the implantation of the electrode. Although the effect of the electrode implantation site can be tested, the guide wire cannot be drawn out after the electrode implantation is finished, if the influence of the device on an experimental body is reduced as much as possible, the guide wire needs to be drawn out after the electrode implantation to perform a stimulation test, the site is verified, and the process easily causes electrode translocation and influences the accuracy of the experiment. In addition, the patent description shows that the electrode tip is gentle and is difficult to insert into the skin of an experimental animal, and certain limitations exist in the experiment.

The chinese patent application with publication number CN108433791A discloses a puncture device system with adjustable needle insertion angle and a control method thereof. Although the operability of the electrode implantation angle is increased, the electrode implantation depth cannot be realized through data visualization, and the feeling and experience judgment of an experimenter are still needed.

The Chinese patent application with publication number CN111529924A provides a deep brain stimulation electrode device, wherein an electrode implantation part adopts a standard needle core needle tube structure, so that an electrode reaches a target brain area; the electrode position is then stabilized by fixing a threaded fixation cannula at the skull puncture site. However, the electrode implantation depth of this device is fixed, and cannot be adjusted temporarily. Meanwhile, the sleeve with larger diameter is fixed on the skull to greatly hurt the experimental animal, and the experimental animal can generate conflict, thereby influencing the experimental result. Therefore, it is necessary to develop a new set of electrode implantation systems, which can achieve data visualization and high implantation accuracy and evacuation of post-implantation auxiliary devices while ensuring less tissue damage. Provides a new method and an operation system for the future animal electrode implantation operation.

Disclosure of Invention

Aiming at the problems, the invention provides an auxiliary electrode implantation system and an electrode implantation method for the adjustable needle insertion depth of a living body, which can solve the problems of insufficient sharpness, no visual data, insufficient precision, incapability of evacuating auxiliary devices and the like in the prior art.

The technical scheme of the invention is as follows: the auxiliary electrode implantation system with the adjustable needle insertion depth comprises an implantation end, an advancing module, an electrode clamping module and a shell 4;

the implanting end comprises a pintle 11 and a needle 12, and the tail end of the needle 12 is detachably connected with the pintle 11;

the propelling module comprises a first-stage propelling device and a second-stage propelling device which are connected with the shell 4, and the pintle 11 is connected on the first-stage propelling device and driven by the first-stage propelling device to do linear reciprocating motion relative to the shell 4; the electrode clamping module is connected to the second-stage propelling device and driven by the second-stage propelling device to do linear reciprocating motion relative to the shell 4, and the electrode wire is clamped by the electrode clamping module.

The shell 4 is provided with a primary scale 41 for representing the displacement of the first-stage propelling device and a secondary scale 42 for representing the displacement of the second-stage propelling device.

An axial through hole 120 is cut in the needle 12 for receiving a wire electrode.

The first-stage propulsion device comprises a threaded pipe 21, a hollow screw 22, a gear driving mechanism 23 and a driving handle 24;

one end of the hollow screw 22 is hinged in the shell 4, the threaded pipe 21 is sleeved with the hollow screw 22 and is in threaded connection with the hollow screw 22, a limiting sliding block 211 is fixedly connected to the outer wall of the threaded pipe 21, a long-strip-shaped sliding hole 212 parallel to the threaded pipe 21 is formed in the shell 4, and the limiting sliding block 211 is connected in the long-strip-shaped sliding hole 212 in a sliding mode;

the gear driving mechanism 23 comprises a driving gear and a driven gear which are meshed with each other, the driving gear is hinged in the shell, and the driven gear is fixedly connected with the hollow screw 22 and has the same axle center with the hollow screw; the driving handle 24 is fixedly connected with the driving gear;

the pintle 11 is fixedly connected to the hollow screw 22, and the primary scale 41 is located on one side of the elongated sliding hole 212.

The second-stage propelling device comprises a bolt 25 and a sleeve 26, a connecting part connected with the casing 4 into a whole is arranged on one side of the casing 4 away from the needle head 22, a threaded blind hole matched with the bolt 25 is formed in the connecting part, and a screw feeding channel communicated with the central part of the hollow screw 22 is formed in the bottom of the threaded blind hole;

the bolt 25 is hollow and is connected in the threaded blind hole through threads, and the electrode clamping module is connected to one side, far away from the connecting part, of the bolt 25;

the sleeve 26 is sleeved on the connecting part, the sleeve 26 is connected with the head of the bolt 25 into a whole, and the secondary scale 42 is arranged on the outer wall of the connecting part.

The electrode clamping module comprises a hollow stud 31, a three-piece clamp 32 and a fastener 33 with internal threads;

the hollow stud 31 is fixedly connected to the end face, opposite to the connecting portion, of the bolt 25, the three-piece clamp 32 is fixedly connected to the hollow stud 31, the fastener 33 is connected to the hollow stud 31 through threads, and a conical groove matched with the three-piece clamp 32 is formed in the fastener 33.

The three-petal clamp 32, the hollow stud 31, the bolt 25, the connecting part, the hollow screw 22, the threaded pipe 21 and the needle 12 are all kept coaxial.

The electrode implantation is carried out according to the following steps:

s1, primary installation;

s1.1, fixing an implementation object, and determining an initial implantation position and a needle type of an electrode;

s1.2, installing an electrode wire in the auxiliary electrode implantation system with the adjustable needle insertion depth;

s1.3, recording the position of the limiting slide block in the primary scale when the needle is completely retracted, and recording as an initial position;

s1.4, calculating the displacement of the needle head according to the initial implantation position of the electrode and the type of the needle head;

s1.5, on the basis of the initial position, combining the displacement calculated in the step S1.4, and driving the needle head to extend out towards one side far away from the shell through the first-stage propelling device, so that the displacement of the limiting slide block reaches the displacement calculated in the step S1.4;

s1.6, zeroing the initial position of the second-stage propelling device;

s1.7, sequentially passing the electrode wire through the three-petal clamp, the first-stage propelling device, the second-stage propelling device and the needle head, and finally keeping the electrode wire flush with the needle tip of the needle head; finally, the fastener is rotated to lock the three-petal clamp;

s2, primary electrode implantation: starting electrode implantation, completely implanting the needle into a living body, and controlling the needle to retract 1-2mm by the first-stage propulsion device, so that the electrode wire is exposed in the experimental object;

s2, testing: testing whether the experimental effect of the current implantation point is good; if yes, go to step S4, otherwise go to step S3;

s3, modifying the depth;

s3.1, keeping the shell still, and adjusting the position of the needle head through the first-stage propelling device;

s3.2, driving the electrode wire to be flush with the needle tip of the needle head through a second-stage propelling device;

s3.3, controlling the needle to retract by 1-2mm through the first-stage propelling device, so that the electrode wire is exposed in the experimental object;

s3.4, returning to the step S2;

s4, withdrawing the needle;

s4.1, controlling the needle to retract outside the experimental object through the first-stage propelling device;

s4.2, the electrode wire exposed out of the skin surface is kept fixed with the experimental object;

s4.3, rotating the fastening piece to loosen the locking of the three-petal clamp;

s4.4, removing the auxiliary electrode implantation system with the adjustable needle insertion depth;

s5, start experiment: and opening external equipment connected with the wire electrode to start the experiment.

The technical scheme of the invention has the following beneficial effects:

the two-stage threaded propulsion module marked with accurate scales is matched with the electrode clamping module, so that data and visualization of the implantation depth of the electrode can be realized, and the reproducibility of the implantation site of the muscle electrode with a good experimental effect is facilitated.

And secondly, the implantation device realizes sharp implantation and short path implantation, and causes less tissue damage.

According to the two-stage propulsion device, multiple tests on the electrical stimulation effects of different depths of the same site can be realized, the stimulation and recording sites with the best effect can be found, and the electrode implantation precision is greatly improved; according to the control method of the system, the implanted electrodes can be prevented from translocation when the auxiliary device is withdrawn, and the accuracy of the experiment is improved while the interference of external equipment is reduced.

Drawings

FIG. 1 is a schematic structural diagram of the present application,

figure 2 is a top view of figure 1,

figure 3 is a cross-sectional view taken along line B-B of figure 1,

figure 4 is a cross-sectional view taken along line a-a of figure 2,

figure 5 is a perspective view of the present case,

FIG. 6 is a flow chart of the present disclosure;

in the figure, 11 is a pintle, 12 is a needle, and 120 is an axial through hole;

21 is a threaded pipe, 211 is a limiting slide block, 212 is a long-strip-shaped slide hole, 22 is a hollow screw, 23 is a gear driving mechanism, 24 is a driving handle, 25 is a bolt, and 26 is a sleeve;

31 is a hollow stud, 32 is a three-piece clip, 33 is a fastener, and 4 is a housing.

Detailed Description

In order to clearly explain the technical features of the present patent, the following detailed description of the present patent is provided in conjunction with the accompanying drawings.

As shown in fig. 1-6, the auxiliary electrode implantation system with adjustable needle insertion depth comprises an implantation end, a propulsion module, an electrode clamping module and a shell 4;

the implanting end comprises a pintle 11 and a needle 12, and the tail end of the needle 12 is detachably connected with the pintle 11; specifically, the needle head is in a hollow cylinder shape, the tail end of the needle head is in an external thread structure, and the pintle is provided with internal threads and can be screwed and connected with the tail end of the needle head;

the propelling module comprises a first-stage propelling device and a second-stage propelling device which are connected with the shell 4, and the pintle 11 is connected on the first-stage propelling device and driven by the first-stage propelling device to do linear reciprocating motion relative to the shell 4; the electrode clamping module is connected to the second-stage propelling device and driven by the second-stage propelling device to do linear reciprocating motion relative to the shell 4, and the electrode wire is clamped by the electrode clamping module.

The shell 4 is provided with a primary scale 41 for representing the displacement of the first-stage propelling device and a secondary scale 42 for representing the displacement of the second-stage propelling device. The position value of the first-level scale, which is closer to the needle, is larger, and the position value of the second-level scale, which is closer to the needle, is larger.

An axial through hole 120 is cut in the needle 12 for receiving a wire electrode.

The first-stage propulsion device comprises a threaded pipe 21, a hollow screw 22, a gear driving mechanism 23 and a driving handle 24;

one end of the hollow screw 22 is hinged in the shell 4, the threaded pipe 21 is sleeved with the hollow screw 22 and is in threaded connection with the hollow screw 22, a limiting sliding block 211 is fixedly connected to the outer wall of the threaded pipe 21, a long-strip-shaped sliding hole 212 parallel to the threaded pipe 21 is formed in the shell 4, and the limiting sliding block 211 is connected in the long-strip-shaped sliding hole 212 in a sliding mode; thus, the threaded pipe 21 can be driven to do linear reciprocating motion relative to the shell by rotating the hollow screw 22;

the gear driving mechanism 23 comprises a driving gear and a driven gear which are meshed with each other, the driving gear is hinged in the shell, and the driven gear is fixedly connected with the hollow screw 22 and has the same axle center with the hollow screw; the driving handle 24 is fixedly connected with the driving gear; thus, when the operator rotates the driving handle 24 during use, the hollow screw 22 is driven by the driving gear and the driven gear to rotate around the axis of the hollow screw, so that the threaded pipe 21 is driven to do linear reciprocating motion relative to the shell;

the pintle 11 is fixedly connected to the hollow screw 22, and the primary scale 41 is located on one side of the elongated sliding hole 212. Thus, the linear movement of the threaded tube 21 will cause the pintle and needle to reciprocate. The primary scale 41 on one side of the strip-shaped sliding hole 212 can intuitively reflect the displacement of the limiting slider and the needle relative to the shell in the process that the limiting slider moves along with the threaded pipe, so that the accuracy is improved, and meanwhile, the visual data of the displacement of the needle is obtained.

The second-stage propelling device comprises a bolt 25 and a sleeve 26, a connecting part connected with the casing 4 into a whole is arranged on one side of the casing 4 away from the needle head 22, a threaded blind hole matched with the bolt 25 is formed in the connecting part, and a screw feeding channel communicated with the central part of the hollow screw 22 is formed in the bottom of the threaded blind hole;

the bolt 25 is hollow and is connected in the threaded blind hole through threads, and the electrode clamping module is connected to one side, far away from the connecting part, of the bolt 25;

the sleeve 26 is sleeved on the connecting part, the sleeve 26 is connected with the head of the bolt 25 into a whole, and the secondary scale 42 is arranged on the outer wall of the connecting part. Therefore, the bolt 25 is screwed to drive the electrode clamping module to do linear reciprocating motion relative to the shell 4, and the secondary scales on the outer wall of the connecting part can visually reflect the displacement of the bolt and the electrode clamping module relative to the shell in the process that the sleeve 26 moves along with the bolt, so that the aim of obtaining visual data of the needle displacement while improving the precision is also fulfilled.

The electrode clamping module comprises a hollow stud 31, a three-piece clamp 32 and a fastener 33 with internal threads;

the hollow stud 31 is fixedly connected to the end face, opposite to the connecting portion, of the bolt 25, the three-piece clamp 32 is fixedly connected to the hollow stud 31, the fastener 33 is connected to the hollow stud 31 through threads, and a conical groove matched with the three-piece clamp 32 is formed in the fastener 33. Therefore, the conical groove can be close to or far from the three-petal clamp by rotating the fastener while the bolt is pressed, so that the three-petal clamp is locked or loosened by the conical groove, and finally the electrode wire penetrating through the three-petal clamp is clamped or loosened by the three-petal clamp.

The three-petal clamp 32, the hollow stud 31, the bolt 25, the connecting part, the hollow screw 22, the threaded pipe 21 and the needle 12 are all kept coaxial. When the needle retracts, the electrode clamping module can ensure that the relative position of the electrode wire and the shell is unchanged, the inner diameter of the needle is slightly larger than that of the electrode wire, the inner diameters of the hollow stud 31, the bolt 25, the connecting part, the hollow screw 22 and the threaded pipe 21 are all larger than that of the needle, the electrode wire is linear inside the device, and the electrode wire is slightly rigid and cannot be easily bent, so that the electrode wire can be effectively prevented from being accidentally displaced when the needle is withdrawn through the optimized structural design.

The electrode implantation is carried out according to the following steps:

s1, primary installation;

s1.1, fixing an implementation object, and determining an initial implantation position and a needle type of an electrode;

s1.2, installing an electrode wire in the auxiliary electrode implantation system with the adjustable needle insertion depth;

s1.3, recording the position of the limiting slide block in the primary scale when the needle is completely retracted, and recording as an initial position;

s1.4, calculating the displacement of the needle head according to the initial implantation position of the electrode and the type of the needle head;

s1.5, on the basis of the initial position, combining the displacement calculated in the step S1.4, and driving the needle head to extend out towards one side far away from the shell through the first-stage propelling device, so that the displacement of the limiting slide block reaches the displacement calculated in the step S1.4;

s1.6, resetting the initial position of the second-stage propelling device to zero, namely adjusting the end face, facing the needle head, of a sleeve in the second-stage propelling device to a starting point in a second-stage scale;

s1.7, sequentially passing the electrode wire through the three-petal clamp, the first-stage propelling device, the second-stage propelling device and the needle head, and finally keeping the electrode wire flush with the needle tip of the needle head; finally, the fastener is rotated to lock the three-petal clamp;

s2, primary electrode implantation: starting electrode implantation, completely implanting the needle into a living body, and controlling the needle to retract 1-2mm by the first-stage propulsion device, so that the electrode wire is exposed in the experimental object;

s2, testing: testing whether the experimental effect of the current implantation point is good; if yes, go to step S4, otherwise go to step S3;

s3, modifying the depth;

s3.1, keeping the shell still, and adjusting the position of the needle head through the first-stage propelling device;

s3.2, driving the electrode wire to be flush with the needle tip of the needle head through a second-stage propelling device;

s3.3, controlling the needle to retract by 1-2mm through the first-stage propelling device, so that the electrode wire is exposed in the experimental object;

s3.4, returning to the step S2;

s4, withdrawing the needle;

s4.1, controlling the needle to retract outside the experimental object through the first-stage propelling device;

s4.2, fixing the electrode wire exposed out of the skin surface with the experimental object by hands or an adhesive tape;

s4.3, rotating the fastening piece to loosen the locking of the three-petal clamp; temporarily disconnecting the connection between the electrode wire and the external device;

s4.4, removing the auxiliary electrode implantation system with the adjustable needle insertion depth;

s5, start experiment: after the connection between the electrode wire and the external equipment is completed again, the external equipment connected with the electrode wire is opened, and the experiment is started.

While the invention has been described in terms of its preferred embodiments, it will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention.