阅读说明：本技术 一种提高水产动物糖利用率的调控组合物、制备方法及与应用 (Regulation and control composition for improving sugar utilization rate of aquatic animals, preparation method and application ) 是由 徐超 李远友 谢帝芝 于 2020-06-22 设计创作，主要内容包括：本发明公开了一种提高水产动物糖利用率的调控组合物,所述调控组合物包含重量份数的组分：黄连素2～30份,姜黄素4～32份和膨化珍珠岩300～530份。该组合物以黄连素和姜黄素为主要原料,充分利用它们生物结构不同的特点进行科学配伍并发挥协同作用。同时,本发明还公开了上述调控物组合物的制备方法与应用。与现有技术相比,该组合物可通过抑制鱼类因摄食高糖饲料而引起的炎症反应,增强鱼体组织胰岛素的敏感性,进而提高鱼类对饲料中糖类的利用率。(The invention discloses a regulation and control composition for improving the utilization rate of sugar of aquatic animals, which comprises the following components in parts by weight: 2-30 parts of berberine, 4-32 parts of curcumin and 300-530 parts of expanded perlite. The composition takes berberine and curcumin as main raw materials, and makes full use of the characteristics of different biological structures of the berberine and curcumin to carry out scientific compatibility and exert synergistic action. Meanwhile, the invention also discloses a preparation method and application of the regulator composition. Compared with the prior art, the composition can enhance the sensitivity of fish tissue insulin by inhibiting the inflammatory reaction of fish caused by eating high-sugar feed, thereby improving the utilization rate of the fish to saccharides in the feed.)

1. A regulation and control composition for improving the utilization rate of sugar of aquatic animals is characterized by comprising the following components in parts by weight:

2-30 parts of berberine;

4-32 parts of curcumin;

300-530 parts of expanded perlite.

2. The conditioning composition according to claim 1, wherein the composition comprises the following components in parts by weight:

10-25 parts of berberine;

5-20 parts of curcumin;

440-500 parts of expanded perlite.

3. The conditioning composition according to claim 2, characterized in that the composition comprises the following components in parts by weight:

13-20 parts of berberine;

6-13 parts of curcumin;

460 portions and 490 portions of expanded perlite.

4. The method for preparing a conditioning composition according to any one of claims 1 to 3, wherein the composition is prepared by pre-pulverizing berberine, curcumin and expanded perlite, respectively, sieving with 120 mesh sieve, and mixing well.

5. The method for preparing a modified composition of claim 4 wherein the berberine and curcumin are milled at a temperature that is above 30 ℃ to avoid high temperatures.

6. Use of the modulating composition of any one of claims 1 to 3 for increasing the sugar availability of an aquatic animal in aquaculture.

7. The use of claim 6, wherein the conditioning composition is added to an aquaculture feed for fish farming.

8. The use of claim 7, wherein the control composition is added to the aquatic feed in an amount of 0.4 to 1.3 wt%.

9. The use as claimed in claim 7 or 8, wherein the fish is megalobrama amblycephala, carp, channel catfish and grass carp.

Technical Field

The invention belongs to the field of aquaculture, and particularly relates to a regulation and control composition for improving the utilization rate of sugar of aquatic animals, and a preparation method and application thereof.

Background

The saccharides are used as important energy sources of biological organisms, have wide sources and low price, and also have the effects of saving protein, protecting the environment and the like. A certain amount of saccharides are added into the feed, so that the growth of the fishes can be promoted, the utilization rate of the feed is improved, and the cost of the feed is obviously reduced. However, the sugar utilization rate of the fish is lower than that of the human and the terrestrial animals, and the excessive addition of the sugar in the feed can cause the phenomena of low disease resistance, slow growth, high death rate and the like of the fish. The symptoms of sugar metabolism dysfunction in fish resemble type II diabetes conditions in humans. Type II diabetes in humans is essentially a chronic inflammatory disease whose inflammatory response is closely related to insulin resistance and islet beta cell death throughout the process of development and progression of the disease. Reducing or inhibiting the inflammatory response of metabolic tissues significantly improves the carbohydrate metabolism function in diabetic model mice. However, the metabolic functions of aquatic animals are different from those of human beings and mammals in efficiency, and whether the metabolic functions of aquatic animals can be improved by reducing or inhibiting the inflammatory reaction of metabolic tissues of the body is not reported in related researches at present. Therefore, the potential mechanism of sugar metabolism disorder of aquatic animals is explored, and the sugar metabolism regulator with anti-inflammatory function is developed, so that the feed additive has obvious production and practical values for reducing feed cost and improving culture economic benefits.

At present, few reports on the development of sugar metabolism regulators for aquatic animals exist, and research mainly focuses on the sugar reducing effect of western medicines. However, the western medicines easily cause the problems of drug resistance, drug residue and the like of aquatic animals in the using process, thereby restricting the wide application of the western medicines in the feed. In recent years, with the rise of applications of natural active substances in the fields of food and medicine, the research on active ingredients of medicinal plants has been increasingly emphasized. Berberine is a quaternary ammonium alkaloid in Chinese goldthread. Curcumin is a polyphenol substance extracted from rhizomes of plants of Zingiberaceae and Araceae. Both of them are proved to improve the glycometabolism function of the diabetes model mouse by inhibiting the synthesis and release of proinflammatory cytokines in tissues such as liver, pancreas and the like of the diabetes model mouse. In aquatic animals, the physiological functions of metabolic tissues or organs are greatly different from those of human and mammals. For example, the liver of fish not only is the center of metabolism of nutrients, but also has the function of insulin synthesis and secretion. So the liver of fish is also called liver pancreas. Whether the two active ingredients of the berberine and the curcumin can enhance the glycometabolism function of the aquatic animals by reducing or inhibiting the inflammatory reaction of the aquatic animals is not reported at present. In addition, the characteristics of the two substances with different biological structures are utilized to carry out scientific compatibility and exert synergistic action to regulate and control the related products of the sugar metabolism function of the aquatic animals, and reports are not found.

Expanded perlite has been widely used in various industrial sectors because of its advantages of stable chemical structure, wide temperature range of use, non-toxicity, tastelessness, low cost, etc. In addition, expanded perlite, due to its porosity, has a strong adsorption capacity and is therefore often used as a carrier material. The fertilizer prepared by using a certain amount of expanded perlite as a carrier can obviously reduce nutrient loss and improve the utilization rate of the fertilizer. However, there are few reports of the application of expanded perlite in feed additives.

In view of the above, the invention researches a regulation and control composition for improving the utilization rate of sugar of aquatic animals by taking expanded perlite as a carrier according to the pharmacological synergistic relationship between the compatibility of berberine and curcumin, and the regulation and control composition can greatly reduce the use amount of protein and fat raw materials in feed and improve the economic benefit of culture when being used as an efficient and environment-friendly aquatic feed additive.

Disclosure of Invention

The invention aims to provide a regulation composition for improving the sugar utilization rate of aquatic animals, a preparation method and application thereof according to the defects of the prior art.

The invention provides a regulation and control composition for improving the utilization rate of sugar of aquatic animals, which adopts the following technical scheme:

a regulation and control composition for improving the utilization rate of sugar of aquatic animals comprises the following components in parts by weight:

2-30 parts of berberine;

4-32 parts of curcumin;

300-530 parts of expanded perlite.

Preferably, the regulation and control composition for improving the sugar utilization rate of the aquatic animals comprises the following components in parts by weight:

10-25 parts of berberine;

5-20 parts of curcumin;

440-500 parts of expanded perlite.

More preferably, the regulation and control composition for improving the sugar utilization rate of the aquatic animals comprises the following components in parts by weight:

13-20 parts of berberine;

6-13 parts of curcumin;

460 portions and 490 portions of expanded perlite.

In another aspect, the present invention provides a method for preparing the above-mentioned modulating composition, which comprises:

the berberine, the curcumin and the expanded perlite in the formula ratio are respectively crushed in advance and then sieved by a 120-mesh sieve, and then the components are fully and uniformly mixed. It is clear that the preparation method of the invention does not need to limit the mixing sequence and the mixing mode, and only needs to fully and uniformly mix the three substances.

Wherein, the berberine and the curcumin need to be prevented from high temperature when being crushed, and the high temperature is more than 30 ℃.

The invention also provides application of the regulation and control composition in aquaculture, particularly application in fish culture, in particular to megalobrama amblycephala, common carp, channel catfish and grass carp. The addition ratio of the regulation and control composition in the aquatic feed formula is 0.4-1.3 wt%, and the regulation and control composition can be adjusted according to the growth stage, specification, cultivation mode and the like of aquatic animals.

Compared with the prior art, the invention has the beneficial effects that:

the regulation and control composition takes plant active ingredients of berberine and curcumin as main raw materials, and makes full use of the characteristics of different biological structures of the berberine and curcumin to carry out scientific compatibility so as to play a synergistic effect. The invention improves the carbohydrate metabolism function of the aquatic livestock by enhancing the immune function of the aquatic livestock, thereby improving the utilization rate of the aquatic livestock to carbohydrates in the feed.

Description of the drawings:

FIG. 1 is the effect of different feeds on the weight gain of young megalobrama amblycephala;

FIG. 2 is the effect of different feeds on the bait coefficient of young megalobrama amblycephala;

FIG. 3 shows the effect of different feeds on the IL-1 β content in the liver of young megalobrama amblycephala;

FIG. 4 is the effect of different feeds on the IL-6 content in the liver of young megalobrama amblycephala;

FIG. 5 shows the effect of different feeds on the expression level of tumor necrosis factor gene in carp liver;

FIG. 6 shows the effect of different feeds on the expression level of interleukin 1 beta gene in carp liver;

figure 7 is the effect of different feeds on glucose tolerance of carp.

The specific implementation mode is as follows:

for a better understanding of the present invention, reference is made to the following drawings and examples, which are not intended to limit the invention in any way. The reagents, methods and apparatus employed in the present invention are conventional in the art unless otherwise indicated. Unless otherwise indicated, reagents and materials used in the present invention are commercially available.