阅读说明：本技术 一种从猕猴桃根中提取半乳糖的方法 (Method for extracting galactose from kiwi fruit root ) 是由 陈红辉 于 2020-07-02 设计创作，主要内容包括：本发明公开了一种从猕猴桃根提取半乳糖的方法,通过将干燥粉末状猕猴桃根水解得到水解液；并将水解液脱色、除盐、过滤后得到半乳糖溶液；最后将得到的半乳糖溶液结晶过滤后干燥得到半乳糖纯品；本工艺原料猕猴桃根易得,成本低,经济效率高；采用聚酰胺进行聚酰胺进行脱色,脱色效果更好；更环保。(The invention discloses a method for extracting galactose from kiwi fruit roots, which comprises the steps of hydrolyzing dry powdery kiwi fruit roots to obtain hydrolysate; decoloring, desalting and filtering the hydrolysate to obtain a galactose solution; finally, crystallizing and filtering the obtained galactose solution, and drying to obtain a pure galactose product; the raw material of the kiwi fruit root is easy to obtain, the cost is low, and the economic efficiency is high; polyamide is adopted for decoloring, so that the decoloring effect is better; is more environment-friendly.)

1. A method for extracting galactose from kiwi fruit roots is characterized by comprising the following steps: hydrolyzing dry powdery kiwi fruit roots to obtain hydrolysate; decoloring, desalting and filtering the hydrolysate to obtain a galactose solution; and finally, crystallizing, filtering and drying the obtained galactose solution to obtain a pure galactose product.

2. The method for extracting galactose from kiwi fruit root according to claim 1, wherein: the method for hydrolyzing the dried powdery kiwi fruit root comprises the following steps: taking 100g of dried powdery kiwi fruit root, adding 1% -3% of dilute sulphuric acid which is 5-10 times of the weight of the dried powdery kiwi fruit root, pressurizing, controlling the temperature to be more than 120 ℃, hydrolyzing for 2-6 h, neutralizing with calcium hydroxide and calcium carbonate, and filtering to obtain hydrolysate.

3. The method for extracting galactose from kiwi fruit root according to claim 1, wherein: the method for decoloring the hydrolysate comprises the following steps: adjusting the concentration of the kiwi fruit root hydrolysate to 2mg/mL, adding polyamide at room temperature, stirring for 60min-90min, and performing static adsorption, wherein the pH value of the hydrolysate is controlled at 5-7.

4. The method for extracting galactose from kiwi fruit root according to claim 1, wherein: the method for desalting the hydrolysate comprises the following steps: desalting the hydrolysate by electrodialysis process.

5. The method for extracting galactose from kiwi fruit root according to claim 1, wherein: and filtering the hydrolysate by adopting a membrane separation process.

6. The method for extracting galactose from kiwi fruit root according to claim 1, wherein: concentrating the galactose solution to the concentration of 0.1-5g/mL, adding 85-95% methanol solution until the volume concentration of methanol is 50-90%, adding seed crystal, stirring and crystallizing; filtering the crystal, and vacuum drying at 60-80 deg.c to obtain pure galactose product.

Technical Field

The invention relates to the technical field of galactose extraction, in particular to a method for extracting galactose from kiwi fruit roots.

Background

The traditional galactose preparation process adopts lactose hydrolysis to obtain D-galactose, has high cost, generates a large amount of waste syrup (glucose), has high environmental protection treatment cost, and the prepared raw materials are animal sources; secondly, the traditional galactose from plant sources is generally prepared from raw materials such as Arabic gum, carrageenan and the like, has high cost, is not a main component and needs to be prepared by separation, or the collagen material contains a large amount of inorganic salt, so that the desalting cost is high and the environmental protection pressure is large.

Disclosure of Invention

The invention aims to provide a method for extracting galactose from kiwi fruit roots, which is used for solving the problems in the background technology.

In order to achieve the purpose, the invention provides the following technical scheme:

a method for extracting galactose from radix Actinidiae chinensis comprises hydrolyzing dry powdered radix Actinidiae chinensis to obtain hydrolysate; decoloring, desalting and filtering the hydrolysate to obtain a galactose solution; and finally, crystallizing, filtering and drying the obtained galactose solution to obtain a pure galactose product.

Preferably, the method for hydrolyzing the dried powdered kiwi fruit root comprises the following steps: taking 100g of dried powdery kiwi fruit root, adding 1% -3% of dilute sulphuric acid which is 5-10 times of the weight of the dried powdery kiwi fruit root, pressurizing, controlling the temperature to be more than 120 ℃, hydrolyzing for 2-6 h, neutralizing with calcium hydroxide and calcium carbonate, and filtering to obtain hydrolysate.

Preferably, the method for decoloring the hydrolysate comprises the following steps: adjusting the concentration of the kiwi fruit root hydrolysate to 2mg/mL, adding polyamide at room temperature, stirring for 60min-90min, and performing static adsorption, wherein the pH value of the hydrolysate is controlled at 5-7.

Preferably, the method for desalting the hydrolysate comprises the following steps: desalting the hydrolysate by electrodialysis process.

Preferably, the hydrolysate is filtered by a membrane separation process.

Specifically, the hydrolyzed solution is filtered through a microporous membrane with the aperture of 0.2-0.8 μm, the filtrate is ultrafiltered through a membrane with the molecular weight cutoff of 3000000Da, the ultrafiltrate is ultrafiltered through a membrane with the molecular weight cutoff of 5000Da, and the galactose solution is obtained through membrane separation and screening.

Preferably, the galactose solution crystallization method comprises the steps of concentrating the galactose solution to the concentration of 0.1-5g/mL, adding 85% -95% methanol solution to the methanol volume concentration of 50% -90%, adding seed crystals, and stirring for crystallization; filtering the crystal, and vacuum drying at 60-80 deg.c to obtain pure galactose product.

Compared with the prior art, the invention has the beneficial effects that:

the invention provides a method for extracting galactose from kiwi fruit roots, and the method has the advantages of easily available raw material kiwi fruit roots, low cost and high economic efficiency; polyamide is adopted for decoloring, so that the decoloring effect is better; is more environment-friendly.

Drawings

FIG. 1 is a chromatogram of galactose according to the present invention.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.