阅读说明：本技术 突变的免疫球蛋白结合多肽 (Mutated immunoglobulin-binding polypeptides ) 是由 G.罗德里戈 M.安德 G.鲍伦 T.布杰克曼 于 2014-07-08 设计创作，主要内容包括：本发明公开了碱性稳定性改进的多肽,所述多肽包含由SEQ ID NO 1或SEQ ID NO 2指定的葡萄球菌A蛋白(SpA)的B或C结构域或由SEQ ID NO 3指定的Z蛋白的突变体,其中至少9位的谷氨酰胺残基突变成天冬酰胺以外的氨基酸。本发明还公开了所述多肽的多聚体以及包含所述多聚体或多肽的分离基质。(The present invention discloses polypeptides with improved alkaline stability comprising a mutant of the B or C domain of staphylococcal protein A (SpA) designated by SEQ ID NO 1 or SEQ ID NO2 or of the protein Z designated by SEQ ID NO 3 wherein at least the glutamine residue at position 9 is mutated to an amino acid other than asparagine. Multimers of the polypeptides and separation matrices comprising the multimers or polypeptides are also disclosed.)