阅读说明：本技术 黑参浓缩液的制备方法 (Preparation method of black ginseng concentrated solution ) 是由 金艳 洪喜道 孙诚 陈琪 王玥珺 于 2020-07-27 设计创作，主要内容包括：本发明公开了黑参浓缩液的制备方法,包括以下步骤：S1：黑参的制备：将样品人参清洗干净,除去杂质,并在自然条件下通风吹干,于40℃干燥12h,蒸制,制备出黑参；S2：在室温下,将黑参与蒸馏水搅拌25至35min,制得第一混合物；S3：在105℃下,将第一混合物放入萃取仪中,并使用萃取剂进行提取,得到提取液；S4：将提取液进行浓缩,得到黑参浓缩液。本发明采用重汤浓缩和减压浓缩两种浓缩方法处理黑参提取液,通过比较两种提取液中总糖含量、总酸性多糖含量、总酚类化合物含量、人参皂苷含量以及感官评价,最终确定更优的浓缩方法,为建立黑参提取液的制作工艺以及优化生产流程提供了帮助。(The invention discloses a preparation method of a black ginseng concentrated solution, which comprises the following steps: s1: preparing black ginseng: cleaning sample ginseng, removing impurities, ventilating and drying under natural conditions, drying at 40 ℃ for 12h, and steaming to prepare black ginseng; s2: stirring the black ginseng and distilled water at room temperature for 25-35 min to prepare a first mixture; s3: placing the first mixture into an extractor at 105 ℃, and extracting with an extractant to obtain an extract; s4: and concentrating the extracting solution to obtain a black ginseng concentrated solution. The black ginseng extracting solution is treated by two concentration methods of heavy soup concentration and reduced pressure concentration, and a better concentration method is finally determined by comparing the total sugar content, the total acidic polysaccharide content, the total phenolic compound content, the ginsenoside content and sensory evaluation in the two extracting solutions, so that help is provided for establishing a manufacturing process and optimizing a production flow of the black ginseng extracting solution.)

1. The preparation method of the black ginseng concentrated solution is characterized by comprising the following steps:

s1: preparing black ginseng: cleaning sample ginseng, removing impurities, ventilating and drying under natural conditions, drying at 40 ℃ for 12h, and steaming to prepare black ginseng;

s2: stirring the black ginseng and distilled water at room temperature for 25-35 min to prepare a first mixture;

s3: placing the first mixture into an extractor at 105 ℃, and extracting with an extractant to obtain an extract;

s4: and concentrating the extracting solution to obtain a black ginseng concentrated solution.

2. The method of claim 1, wherein the step S1 further comprises a pre-heating step after drying, wherein the dried ginseng is pre-heated by steam at 100 ℃ for 2 hours.

3. The method for preparing black ginseng powder according to claim 1 or 2, wherein the preheated ginseng is smoothly placed in a steam sterilizer at 95 ℃ and steamed for 3 hours by heating.

4. The method of preparing a black ginseng concentrate according to claim 3, wherein the specific gravity of the black ginseng and the distilled water in the first mixture is 1g per 20mL of the distilled water.

5. The method for preparing a black ginseng concentrate according to claim 4, wherein the extracting agent is selected from distilled water, the extracting time is 3 hours, and the extracting times are 1 to 3 times.

6. The method of preparing a black ginseng concentrate according to claim 5, wherein the content of soluble solid black ginseng in the black ginseng concentrate is 60 ° Brix.

7. The method for preparing a black ginseng concentrate according to claim 6, wherein the concentration comprises concentration under reduced pressure, wherein the vacuum degree is controlled to be-0.07 to-0.09 MPa and the heating temperature is 60 ℃ during the reduced pressure.

8. The method of preparing a black ginseng concentrate according to claim 6, wherein the concentrating further comprises a re-broth concentrating, the re-broth concentrating step comprising:

and (3) placing the extracting solution into a small steamer, placing the small steamer with the extracting solution into a water bath kettle at the temperature of 100 ℃, and concentrating the heavy soup.

Technical Field

The invention relates to the technical field of ginseng processing, in particular to a preparation method of a black ginseng concentrated solution.

Background

The ginseng is sweet and slightly bitter in taste and slightly warm in nature, has the effects of tonifying five internal organs, beautifying, soothing the nerves, enhancing immunity, resisting cancer, stopping fright, reducing blood sugar, removing pathogenic qi, reducing blood fat, improving eyesight, benefiting intelligence and prolonging life after being taken for a long time, and has extremely high medicinal value.

The black ginseng is a novel ginseng processed product obtained by a processing method of nine steams and nine aerations, contains no or less rare ginsenoside, polysaccharide, flavonoid and the like in other ginseng processed products, and has higher biological activity of resisting inflammation, resisting oxidation and the like.

Compared with red ginseng and white ginseng, the black ginseng is rarely researched at home and abroad, and the quality control standard of the black ginseng is yet to be established. So far, the processing technology standard (mainly the time and temperature of steaming and drying) of the black ginseng is not agreed at home and abroad, different people use different processing technologies, the most widely used technology is steaming for 2-3 h at 98 ℃, then airing, and repeating for nine times. Meanwhile, the chemical components and pharmacological actions of black ginseng still need to be continuously researched, and most of the black ginseng is concentrated on ginsenoside at present.

The black ginseng can be directly eaten, and can also be used in production and life in the form of black ginseng extract or black ginseng fermentation liquor. The black ginseng extract has stable components, definite content and convenient preparation, thereby becoming the main sale and use form of the black ginseng, and simultaneously, the black ginseng extract has a plurality of medicinal activities. However, the manufacturing process and the optimized production flow of the black ginseng extract have defects.

Disclosure of Invention

It is an object of the present invention to provide a process for the preparation of a black ginseng concentrate, which solves one or more of the above mentioned problems of the prior art.

The preparation method of the black ginseng concentrated solution provided by the invention comprises the following steps:

s1: preparing black ginseng: cleaning sample ginseng, removing impurities, ventilating and drying under natural conditions, drying at 40 ℃ for 12h, and steaming to prepare black ginseng;

s2: stirring the black ginseng and distilled water at room temperature for 25-35 min to prepare a first mixture;

s3: placing the first mixture into an extractor at 105 ℃, and extracting with an extractant to obtain an extract;

s4: and concentrating the extracting solution to obtain a black ginseng concentrated solution.

In some embodiments, the step S1 further includes a pre-heating step after drying, in which the dried ginseng is pre-heated by steam at 100 ℃ for 2 hours.

In some embodiments, the preheated ginseng is flatly placed in a steam sterilizer at 95 ℃ and steamed for 3 hours.

In some embodiments, the steamed ginseng sample is dried in an oven at 40 ℃ for 12h, and repeated 9 times to produce black ginseng.

In certain embodiments, the specific gravity of the black ginseng and distilled water in the first mixture is 1g per 20mL of distilled water.

In certain embodiments, the extractant is selected from distilled water, the extraction time is 3 hours, and the number of extractions is 1 to 3.

In certain embodiments, the concentration of soluble solid black ginseng in the black ginseng concentrate is 60 ° Brix.

In some embodiments, the concentrating comprises concentrating under reduced pressure, wherein the vacuum degree is controlled to be-0.07 to-0.09 MPa and the heating temperature is 60 ℃ during the reduced pressure.

In some embodiments, the concentrating further comprises re-broth concentrating, the step of re-broth concentrating comprising:

placing the extractive solution in a small steamer, placing the small steamer in a water bath at 100 deg.C, and concentrating.

Has the advantages that: the black ginseng extracting solution is treated by two concentration methods of heavy soup concentration and reduced pressure concentration, and a better concentration method is finally determined by comparing the total sugar content, the total acidic polysaccharide content, the total phenolic compound content, the ginsenoside content and sensory evaluation in the two extracting solutions, so that help is provided for establishing a manufacturing process and optimizing a production flow of the black ginseng extracting solution.

Drawings

FIG. 1 is a glucose standard curve according to the present invention;

FIG. 2 is a graph comparing the total sugar content of two black ginseng concentrates according to the present invention;

FIG. 3 is a galacturonic acid standard curve according to the present invention;

FIG. 4 is a graph comparing the content of acidic polysaccharides in two concentrated solutions of black ginseng according to the present invention;

FIG. 5 is a gallic acid standard curve according to the present invention;

FIG. 6 is a graph comparing the total phenolic compound content in two black ginseng concentrates according to the present invention;

fig. 7 is a sensory evaluation analysis chart of two black ginseng concentrates according to the present invention.

Detailed Description

The present invention will be described in further detail below with reference to embodiments.

Materials and reagents

The ginseng is purchased from the Changbai mountain ginseng market in Wanliang;

glucose, galacturonic acid, gallic acid were purchased from Sigma chemical Co (stouis, MO, USA); ginsenoside standards (Rg1, Re, Rf, Rh1(S), Rg2(S), Rg2(R), Rb1, Rc, Rb2, Rb3, Rd, Rk3, Rh4, Rg3(S), Rg3(R), Rg5) were purchased from kywmasts; UPLC analytical solvents such as water, acetonitrile, etc. were purchased from Merk (germany); reagents such as phenol, concentrated sulfuric acid, ethanol, carbazole acid, folin phenol reagent, sodium carbonate, etc. were used in GPR grade.

(II) Instrument

Ultra high performance liquid chromatography (UPLC, available from Hitachi, japan); c18 column (5 μm, 4.6X 250mm, from Waters); 0.2 μm filters (from Whatman); uv/vis spectrophotometer (available from shanghai chromatography instruments ltd); centrifuge (from Eppendorf, germany). Electronic balance CB-1200 (available from CAS, Korea); sample application pipettor (purchased from Eppendorf, germany).