阅读说明：本技术 一种生物解剖实验用防腐剂及制备方法 (Preservative for biological dissection experiments and preparation method thereof ) 是由 郭红芳 崔光辉 黄银宝 贾焕霞 刘其伟 于 2020-07-13 设计创作，主要内容包括：本发明公开了一种生物解剖实验用防腐剂及制备方法,由大黄、桂皮、厚朴、虎耳草、聚乙二醇、异十六烷和聚醚改性有机硅制成。与现有技术相比,本发明主要采用中药材提取物构成,配以少量的化学成分,使得本发明的构成的防腐剂具有良好的防腐效果,且本发明的防腐剂对生物标本表面损伤小,不易变质,使得实验时间延长,生物标本不易氧化损坏,具有推广应用的价值。(The invention discloses a preservative for biological anatomy experiments and a preparation method thereof. Compared with the prior art, the preservative mainly comprises the traditional Chinese medicinal material extract, and is matched with a small amount of chemical components, so that the preservative has a good preservative effect, has small damage to the surface of a biological specimen, is not easy to deteriorate, prolongs the experimental time, is not easy to oxidize and damage the biological specimen, and has popularization and application values.)

1. The preservative for the biological dissection experiment is characterized in that: is prepared from rhubarb, cassia bark, magnolia bark, saxifrage, polyethylene glycol, isohexadecane and polyether modified organic silicon.

2. The preservative for use in biological dissection experiments according to claim 1, wherein: according to parts by weight, each part comprises 20-30% of the rhubarb, 2-30% of the cassia bark, 15-20% of the magnolia bark, 15-25% of the saxifrage, 3-5% of the polyethylene glycol, 4-7% of the isohexadecane and 4-7% of the polyether modified organic silicon.

3. The preservative for use in biological dissection experiments according to claim 2, wherein: according to the weight portion, each portion comprises 25% of rhubarb, 25% of cassia bark, 15% of magnolia officinalis, 20% of saxifrage, 5% of polyethylene glycol, 5% of isohexadecane and 5% of polyether modified organic silicon.

4. A preparation method of preservative for biological dissection experiments is characterized by comprising the following steps:

s1: preparing rhubarb, cassia bark, magnolia officinalis and saxifrage according to the parts by weight;

s2: extracting with 40-60% ethanol solution to obtain ethanol extractive solution;

s3: and (4) adding polyethylene glycol, isohexadecane and polyether modified organic silicon into the ethanol extract obtained in the step S2, and stirring and mixing uniformly to obtain the preservative.

5. The method for preparing preservative for use in biological dissection experiments according to claim 2, wherein: the weight of the ethanol solution is 2.5 times of the total weight of the rhubarb, the cassia bark, the officinal magnolia bark and the saxifrage.

6. The method for preparing preservative for use in biological dissection experiments according to claim 2, wherein: and in the step S3, heating the ethanol extract to 60-70 ℃, adding polyethylene glycol, isohexadecane and polyether modified organic silicon, and stirring.

7. The method for preparing preservative for use in biological dissection experiments according to claim 6, wherein: the stirring speed is 500-800 r/min.

Technical Field

The invention relates to the technical field of biology, in particular to a preservative for a biological dissection experiment and a preparation method thereof.

Background

Dissection is an intensive analytical study of things. Meanwhile, dissection is also a medical and biological term, which means that a special knife or scissors is used to dissect a human body or animal and plant bodies. Among the prior art, in to biological research, especially, the anatomical experiment of animal body, tissue can be oxidized after dissecting, can corrode very fast, and the experiment that can not accomplish in the short time will lead to dissected pathological change tissue to corrode very fast, thereby influence the experimental effect, influence the experimental result degree of accuracy, prior art adopts freezing mostly, and freezing reduces the corrosion rate, but the tissue can be freeze-dried or freeze rotten, and present antiseptic is formaldehyde solution mostly, formaldehyde is not only harmful to the human body easily, and can lead to experimental tissue rotten, thereby influence the experimental result, consequently, there is the space of improvement.

Disclosure of Invention

The invention aims to solve the problems and provide a preservative for biological anatomy experiments and a preparation method thereof.

The invention realizes the purpose through the following technical scheme:

the preservative for the biological dissection experiment is prepared from rhubarb, cassia bark, magnolia bark, saxifrage, polyethylene glycol, isohexadecane and polyether modified organic silicon.

Further, each part by weight comprises 20-30% of rheum officinale, 2-30% of cinnamon, 15-20% of mangnolia officinalis, 15-25% of saxifrage, 3-5% of polyethylene glycol, 4-7% of isohexadecane and 4-7% of polyether modified organic silicon.

Preferably, each part comprises, by weight, 25% of the rheum officinale, 25% of the cinnamon, 15% of the magnolia officinalis, 20% of the saxifrage, 5% of the polyethylene glycol, 5% of the isohexadecane and 5% of the polyether modified silicone.

The invention relates to a preparation method of a preservative for a biological dissection experiment, which comprises the following steps:

s1: preparing rhubarb, cassia bark, magnolia officinalis and saxifrage according to the parts by weight;

s2: extracting with 40-60% ethanol solution to obtain ethanol extractive solution;

s3: and (4) adding polyethylene glycol, isohexadecane and polyether modified organic silicon into the ethanol extract obtained in the step S2, and stirring and mixing uniformly to obtain the preservative.

Specifically, the weight of the ethanol solution is 2.5 times of the total weight of the rhubarb, the cassia bark, the officinal magnolia bark and the saxifrage.

Preferably, in the step S3, the ethanol extract is heated to 60 to 70 ℃, and then polyethylene glycol, isohexadecane and polyether modified organic silicon are added and stirred.

Preferably, the stirring speed is 500-.

The invention has the beneficial effects that:

compared with the prior art, the preservative mainly comprises traditional Chinese medicine extracts and a small amount of chemical components, so that the preservative has a good preservative effect, has small damage to the surface of a biological specimen, is not easy to deteriorate, prolongs the experimental time, is not easy to oxidize and damage the biological specimen, and has popularization and application values.

Detailed Description

The invention is further illustrated below:

the preservative for the biological dissection experiment is prepared from rhubarb, cassia bark, magnolia bark, saxifrage, polyethylene glycol, isohexadecane and polyether modified organic silicon.

Further, each part by weight comprises 20-30% of rheum officinale, 2-30% of cinnamon, 15-20% of mangnolia officinalis, 15-25% of saxifrage, 3-5% of polyethylene glycol, 4-7% of isohexadecane and 4-7% of polyether modified organic silicon.

Preferably, each part comprises, by weight, 25% of the rheum officinale, 25% of the cinnamon, 15% of the magnolia officinalis, 20% of the saxifrage, 5% of the polyethylene glycol, 5% of the isohexadecane and 5% of the polyether modified silicone.

The invention relates to a preparation method of a preservative for a biological dissection experiment, which comprises the following steps:

s1: preparing rhubarb, cassia bark, magnolia officinalis and saxifrage according to the parts by weight;

s2: extracting with 40-60% ethanol solution to obtain ethanol extractive solution;

s3: and (4) adding polyethylene glycol, isohexadecane and polyether modified organic silicon into the ethanol extract obtained in the step S2, and stirring and mixing uniformly to obtain the preservative.

Specifically, the weight of the ethanol solution is 2.5 times of the total weight of the rhubarb, the cassia bark, the officinal magnolia bark and the saxifrage.

Preferably, in the step S3, the ethanol extract is heated to 60 to 70 ℃, and then polyethylene glycol, isohexadecane and polyether modified organic silicon are added and stirred.

And (3) experimental verification:

the preservative for the biological dissection experiment prepared by the invention is coated on the surface of fresh pork, the coating amount of 1kg of pork is 20g, a fresh injection without any preservative is prepared for comparison, and the daily measured data is as follows:

table 1: no preservative is used

Table 2: preservative for biological dissection experiments prepared by using the invention

As can be seen from tables 1 and 2, the present invention has a good corrosion prevention effect

The foregoing shows and describes the general principles and features of the present invention, together with the advantages thereof. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are described in the specification and illustrated only to illustrate the principle of the present invention, but that various changes and modifications may be made therein without departing from the spirit and scope of the present invention, which fall within the scope of the invention as claimed. The scope of the invention is defined by the appended claims and equivalents thereof.