阅读说明：本技术 一种用于提高免疫力的脾多肽组合物及口服液 (Spleen polypeptide composition for improving immunity and oral liquid ) 是由 浦同骄 彭明嫒 徐顺意 何来鹏 陈润 齐兴忠 于 2020-07-10 设计创作，主要内容包括：本发明涉及脾多肽制品技术领域,具体公开一种用于提高免疫力的脾多肽组合物以及其制备的口服液。所述脾多肽组合物由动物脾脏经细胞破碎、酶解、脱脂和过滤等工序制得,有效成分含量高,脂肪含量低,具有提高免疫力功效,且使用安全,无健康隐患。由所述脾多肽组合物和酪蛋白或其盐制成的脾多肽口服液,提高免疫力、抗肿瘤及抗肺炎等功效得到了进一步的提高,并且具有良好的贮存稳定性。(The invention relates to the technical field of spleen polypeptide products, and particularly discloses a spleen polypeptide composition for improving immunity and an oral liquid prepared from the spleen polypeptide composition. The spleen polypeptide composition is prepared from animal spleens through the working procedures of cell disruption, enzymolysis, degreasing, filtering and the like, has high content of effective components and low fat content, has the effect of improving immunity, and is safe to use without hidden health hazards. The spleen polypeptide oral liquid prepared from the spleen polypeptide composition and casein or salt thereof has the effects of improving immunity, resisting tumor, resisting pneumonia and the like, is further improved, and has good storage stability.)

1. A spleen polypeptide composition for improving immunity is characterized by being prepared by the following steps: taking spleen of a mammal, carrying out cell disruption to obtain cell disruption solution, then adding protease for hydrolysis, adding a degreasing agent into hydrolysate after the hydrolysis is finished to remove fat and cholesterol, and finally carrying out solid-liquid separation to obtain spleen polypeptide composition solution; the cell disruption is to mechanically crush the spleen of the mammal to a particle size of less than 2mm, and then carry out cell disruption by adopting a freezing-crushing-thawing method, wherein the freezing temperature is-80 to-40 ℃, the thawing temperature is 0 to 20 ℃, the freezing-crushing-thawing is carried out for 1 to 3 times, and the particle size of the material is less than 500 mu m; the degreasing agent is kaolin, diatomite or beta-cyclodextrin, and the dosage of the degreasing agent is 1-9% of the mass of the hydrolysate.

2. The spleen polypeptide composition of claim 1, wherein the protease is pepsin, trypsin, chymotrypsin or papain, the dosage of the protease is 0.01-10% of the mass of the cell disruption solution, the enzymolysis temperature is 20-80 ℃, and the enzymolysis time is 0.5-40 h.

3. An oral liquid of spleen polypeptide for improving immunity, which comprises the spleen polypeptide composition of claim 1 or 2 and casein or a salt thereof, wherein the mass ratio of the spleen polypeptide composition to the casein is 1: 0.1 to 25.

4. The spleen polypeptide oral liquid of claim 3, wherein the concentration of the spleen polypeptide composition is 5-100 g/L.

5. The spleen polypeptide oral liquid of claim 4, further comprising acceptable raw and auxiliary materials in food industry and/or pharmaceutical preparations.

6. The spleen polypeptide oral liquid of claim 5, which is prepared by the following steps: and adding the casein or the salt thereof and the auxiliary materials into the spleen polypeptide composition solution, adjusting the concentration, preparing into a solution, filling the solution into a beverage container, and sterilizing to obtain the spleen polypeptide oral liquid.

7. The spleen polypeptide oral liquid of claim 6, wherein the sterilization is performed at 110-135 ℃ for 1-40 min.

Technical Field

The invention relates to the technical field of spleen polypeptide products, in particular to a spleen polypeptide composition and oral liquid for improving immunity.

Background

The spleen is also called as the shagan and is an important viscera of an animal body, and the spleen of domestic animals such as pigs, cattle, sheep and the like contains various nutrient substances, so that the spleen can be used as a medicine-food homologous food from ancient times and has extremely high food therapy value. In modern technology, spleen is extracted to obtain spleen polypeptide, which is a polypeptide composition containing various small molecular peptides, amino acids and nucleotides and has positive effect on the immune system of human body.

Although the spleen is rich in nutrients, it also contains a large amount of fat and cholesterol, for example, the contents of fat and cholesterol in the spleen of every 100g pig are 2.6g and 363mg, respectively. The intake of a large amount of cholesterol easily causes the increase of the content of blood cholesterol, thereby causing diseases such as atherosclerosis, myocardial infarction, coronary heart disease and the like; high-fat food is intensively eaten in a short time, so that the mental work can be influenced, the artery blood vessel can be blocked, the function of the brain can be damaged, and hearing loss can be easily caused by hearing damage after the high-fat food is eaten for a long time. Therefore, how to remove fat and cholesterol efficiently when extracting spleen polypeptide from spleen has important significance on the quality and efficacy of spleen polypeptide products.

Disclosure of Invention

In view of the technical current situation, the invention provides a spleen polypeptide composition for improving immunity and provides an oral liquid prepared from the spleen polypeptide composition.

In order to achieve the purpose, the invention adopts the following technical scheme:

a spleen polypeptide composition for improving immunity is prepared by the following steps: taking spleen of a mammal, carrying out cell disruption to obtain cell disruption solution, then adding protease for hydrolysis, adding a degreasing agent into hydrolysate after the hydrolysis is finished to remove fat and cholesterol, and finally removing the degreasing agent to obtain spleen polypeptide composition solution; the cell disruption is to mechanically crush the spleen of the mammal to a particle size of below 500 mu m, then to perform freezing treatment by a freeze-thaw method, and to perform repeated crushing and freeze-thaw processes to complete cell disruption, wherein the freeze-thaw method has a freezing temperature of-80 to-40 ℃, a thawing temperature of 0 to 20 ℃, repeated crushing and freeze-thaw times of 0 to 3 times; the degreasing agent is kaolin, diatomite or beta-cyclodextrin, and the dosage of the degreasing agent is 1-9% of the mass of the hydrolysate.

The spleen polypeptide composition prepared by the invention has the immunoregulation effect, stimulates immune cells in a specific mode, enhances the immune response function, maintains normal and moderate immune response, has the obvious effect of improving the immunity, has obvious effects on children respiratory diseases, pneumonia, cancers and the like, and can be used for preparing foods, health-care foods or medicines with related functions. The cell is broken under specific conditions, and then the specific degreasing agent is selected, so that fat and cholesterol can be removed to the maximum extent, and the polypeptide content is increased. In the spleen polypeptide composition solution, the mass content of the polypeptide is more than 85% (calculated on a dry basis), the total fat content is less than 0.5g/100ml, the cholesterol content is less than 5mg/100ml, and the spleen polypeptide composition solution can be safely eaten without hidden health hazards.

As a specific embodiment of the invention, the protease is pepsin, trypsin, chymotrypsin or papain, the dosage of the protease is 0.01-10% of the mass of the cell disruption solution, the enzymolysis temperature is 20-80 ℃, and the enzymolysis time is 0.5-40 h.

The invention also provides spleen polypeptide oral liquid, which comprises the spleen polypeptide composition and casein or a salt thereof, wherein the mass ratio of the spleen polypeptide composition to the casein or the salt thereof is 1: 0.1 to 25. The mass of the spleen polypeptide composition is calculated by multiplying the mass of nitrogen by 6.25, as follows.

The spleen polypeptide composition is prepared into oral liquid, is convenient to take, and meets the daily requirement of people for taking as food, health-care products or medicines. The spleen polypeptide is easy to degrade and lose activity under the action of digestive juice after being orally taken, which is also the main reason that most of the spleen polypeptide products are injection drugs at present. The casein salt can be potassium, sodium, magnesium or calcium salt, the casein and the casein are safe food raw materials, no side effect is caused when the casein or the casein salt is added into the spleen polypeptide oral liquid, the casein or the casein salt has the effects of improving the immunity, promoting the calcium absorption and the like, and the casein or the casein salt is complementary with the spleen polypeptide, so that the effect of the product can be further improved. In addition, casein or casein salt is added into the spleen polypeptide oral liquid, so that the polymerization of polypeptide molecules can be effectively inhibited, the storage stability of the product is improved, and the shelf life of the product is prolonged.

In a specific embodiment of the invention, the mass concentration of the spleen polypeptide composition in the spleen polypeptide oral liquid is 5-100 g/L.

As a specific embodiment of the invention, the spleen polypeptide oral liquid also comprises auxiliary materials allowed in food industry and/or pharmaceutical preparations, such as nutrition enhancers, antioxidants, thickeners, acidity regulators, sweeteners, preservatives, pigments, essences and the like, and other raw materials which do not influence the performance of spleen polypeptides and casein.

As a specific embodiment of the present invention, the preparation method of the spleen polypeptide oral liquid comprises: and adding the casein or the salt thereof and the auxiliary materials into the spleen polypeptide composition solution, adjusting the concentration, preparing into a solution, filling the solution into a beverage container, and sterilizing to obtain the spleen polypeptide oral liquid.

In a specific embodiment of the invention, the sterilization is performed at 110-135 ℃ for 1-40 min. Under the condition, the sterilization effect is ensured, and the influence on the effective components is reduced to the maximum extent.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.