阅读说明：本技术 一种用于促愈抗感染的三层环状结构可吸收手术缝合线的制作方法 (Method for manufacturing absorbable surgical suture with three-layer annular structure for healing promotion and infection resistance ) 是由 于洋 马心怡 孙与泽 韩乔松 王梦琪 陈一诺 袁颢宸 张稚平 于 2020-07-30 设计创作，主要内容包括：本发明公开了一种用于促愈抗感染的三层环状结构可吸收手术缝合线的制作方法,包括以下步骤,分别采用1.0mol/L HCI溶液和50％NaOH溶液处理甲壳,提取出甲壳素,用50％NaOH溶液在温度90℃、保温时间3h环境下处理甲壳素脱乙酰基制备壳聚糖原液,通过与氯乙酸进行羧甲基化反应制备2份羧甲基壳聚糖,作为最外层。将壳聚糖溶解于5％～6％的稀乙酸溶液中,添加尿素、乙酸锌作为助剂利用搅拌装置进行搅拌,制备壳聚糖的纺丝原液,凝固、拉伸、洗涤,在张力状态下于70℃利用干燥装置干燥1h。本发明通过控制三者的质量比达到控制降解速度的目的,使缝合线的降解速率与伤口愈合速率一致,采用PLC控制模式,调整喷丝速度,使缝线结构稳定均匀,降低了机体发生异物反应的概率。(The invention discloses a method for manufacturing a three-layer annular structure absorbable surgical suture line for healing promotion and infection resistance, which comprises the following steps of respectively adopting 1.0mol/L HCI solution and 50% NaOH solution to treat a shell, extracting chitin, treating chitin deacetylation with 50% NaOH solution at the temperature of 90 ℃ for 3h to prepare chitosan stock solution, and carrying out carboxymethylation reaction with chloroacetic acid to prepare 2 parts of carboxymethyl chitosan serving as an outermost layer. Dissolving chitosan in 5-6% dilute acetic acid solution, adding urea and zinc acetate as auxiliary agents, stirring by using a stirring device, preparing a spinning solution of chitosan, solidifying, stretching, washing, and drying for 1h at 70 ℃ under a tension state by using a drying device. The invention achieves the purpose of controlling the degradation speed by controlling the mass ratio of the three components, so that the degradation speed of the suture is consistent with the wound healing speed, and the spinning speed is adjusted by adopting a PLC control mode, so that the suture structure is stable and uniform, and the probability of the organism generating foreign body reaction is reduced.)

1. A method for manufacturing a three-layer ring structure absorbable surgical suture for promoting healing and resisting infection is characterized by comprising the following steps of:

s1: respectively adopting 1.0mol/L HCI solution and 50% NaOH solution to treat the carapace to extract chitin, treating chitosan deacetylation with 50% NaOH solution at 90 ℃ for 3h to prepare chitosan stock solution, and performing carboxymethylation reaction with chloroacetic acid to prepare 2 parts of carboxymethyl chitosan as an outermost layer;

s2: dissolving chitosan in 5-6% dilute acetic acid solution, adding urea and zinc acetate as auxiliaries, stirring by using a stirring device to prepare a spinning stock solution of chitosan, solidifying, stretching, washing, and drying for 1h by using a drying device at 70 ℃ under a tension state to finally obtain 3 parts of original-state chitosan fiber core with sufficient mechanical strength;

s3: cutting Os Sepiae into pieces of 1-3cm, adding appropriate amount of pepsin and acetic acid solution, standing, filtering the extractive solution, adding NaCl to final concentration of 10%, centrifuging at 1500r/min to obtain collagen precipitate, dissolving with 0.5mo/L acetic acid, and dialyzing to obtain purified collagen solution;

s4: preparing a saturated collagen solution at room temperature, putting the saturated collagen solution into the original state chitosan, soaking for 3min, airing, crosslinking by using glutaraldehyde with the concentration of 0.25%, washing by using distilled water, and dehydrating to obtain crosslinked original state chitosan-collagen with the mass ratio of 1: 1;

s5: degumming 1 part of raw silkworm silk in 15L of boiling Na2CO3(0.02M) aqueous solution for 30 minutes, washing with deionized water, carrying out air drying by using an air drying device, weighing dried silkworm fibroin fiber, dissolving in 120mL of LiBr (9.3M) solution, dissolving in a 65 ℃ oven, taking out the solution, pouring into a dialysis bag, dialyzing in deionized water for 36 hours, centrifuging the dialyzed solution twice in a high-speed centrifuge at 9000r/min, removing impurities, finally obtaining 1 part of fibroin protein solution, and drying to obtain a berberine-fibroin protein antibacterial coating material;

s6: and (3) putting the sample into 1 part of berberine-silk fibroin mixed solution for dipping to obtain an antibacterial coating, and then controlling a spinning device by the semi-finished product through a PLC (programmable logic controller) technology to obtain a finished product.

2. The method for preparing the absorbable surgical suture with the three-layer annular structure for promoting healing of infection resistance according to claim 1 is implemented by a processing system, wherein the processing system comprises a stirring device, a drying device, an air drying device and a spinning device, and the spinning device comprises a spinning machine and a spinning device.

3. The method for preparing the absorbable surgical suture with the three-layer ring structure for promoting healing and resisting infection as claimed in claim 2, wherein the stirring device comprises a stirring box, a stirring motor is fixed on the outer wall of the top of the stirring box through a screw, and an output shaft of the stirring motor is connected with a stirring rod through a coupling.

4. The method for preparing the absorbable surgical suture with the three-layer ring structure for promoting healing and resisting infection as claimed in claim 3, wherein the drying device comprises a drying oven, heating wires are arranged on the inner walls of two sides of the drying oven, and a temperature sensor is fixed on the inner wall of the top of the drying oven through screws.

5. The method for preparing an absorbable surgical suture with three layers of ring structures for promoting healing of infection according to claim 4, wherein the air drying device comprises an air blowing cylinder, a support is arranged on the inner wall of the air blowing cylinder, a motor is fixed on the outer wall of the support through screws, and fan blades are connected to the output shaft of the motor through a coupling.

6. The method for preparing the absorbable surgical suture with the three-layer ring structure for promoting healing and resisting infection as claimed in claim 1, wherein 1 part of berberine powder is weighed instead of S5, and the berberine powder is dissolved in deionized water to prepare a berberine solution with the concentration of 4 mg/mL. Adding 0.5 part of glycerol into the silk fibroin solution, uniformly mixing the berberine solution and the silk fibroin glycerol mixed solution, wherein the mass ratio of silk fibroin to berberine is 10:1, and drying to obtain the berberine-silk fibroin antibacterial coating material.

Technical Field

The invention relates to the technical field of surgical sutures, in particular to a manufacturing method of an absorbable surgical suture with a three-layer annular structure for promoting healing and resisting infection.

Background

The surgical suture line is a special line for ligation hemostasis, suture hemostasis and tissue suture in the surgical operation or the trauma treatment, and is divided into absorbable lines and non-absorbable lines, wherein the absorbable surgical line on the market is mainly composed of artificially synthesized components, and the surgical line partially adopts mixed components, has no microstructure, and is woven by simply mixing the components into silk.

The prior domestic absorbable suture line product has the disadvantages of rough preparation method, uneven thickness of the suture line and poor structural stability, the specifications of the absorbable suture line product are not uniform, the absorbable suture line product cannot meet pharmacopoeia standards, and obviously the absorbable suture line product cannot meet the use requirements of people.

Disclosure of Invention

Based on the technical problems of the prior domestic absorbable suture line product in the background technology, such as rough process, uneven thickness of the suture line, poor structural stability, non-uniform specification and incapability of meeting pharmacopoeia standards, the invention provides a method for manufacturing a three-layer annular-structure absorbable surgical suture line for promoting healing and resisting infection.

The invention provides a method for manufacturing an absorbable surgical suture with a three-layer annular structure for promoting healing and resisting infection, which comprises the following steps:

s1: respectively adopting 1.0mol/L HCI solution and 50% NaOH solution to treat the carapace to extract chitin, treating chitosan deacetylation with 50% NaOH solution at 90 ℃ for 3h to prepare chitosan stock solution, and performing carboxymethylation reaction with chloroacetic acid to prepare 2 parts of carboxymethyl chitosan as an outermost layer;

s2: dissolving chitosan in 5-6% dilute acetic acid solution, adding urea and zinc acetate as auxiliaries, stirring by using a stirring device to prepare a spinning stock solution of chitosan, solidifying, stretching, washing, and drying for 1h by using a drying device at 70 ℃ under a tension state to finally obtain 3 parts of original-state chitosan fiber core with sufficient mechanical strength;

s3: cutting Os Sepiae into pieces of 1-3cm, adding appropriate amount of pepsin and acetic acid solution, standing, filtering the extractive solution, adding NaCl to final concentration of 10%, centrifuging at 1500r/min to obtain collagen precipitate, dissolving with 0.5mo/L acetic acid, and dialyzing to obtain purified collagen solution;

s4: preparing a saturated collagen solution at room temperature, putting the saturated collagen solution into the original state chitosan, soaking for 3min, airing, crosslinking by using glutaraldehyde with the concentration of 0.25%, washing by using distilled water, and dehydrating to obtain crosslinked original state chitosan-collagen with the mass ratio of 1: 1;

s5: degumming 1 part of raw silkworm silk in 15L of boiling Na2CO3(0.02M) aqueous solution for 30 minutes, washing with deionized water, carrying out air drying by using an air drying device, weighing dried silkworm fibroin fiber, dissolving in 120mL of LiBr (9.3M) solution, dissolving in a 65 ℃ oven, taking out the solution, pouring into a dialysis bag, dialyzing in deionized water for 36 hours, centrifuging the dialyzed solution twice in a high-speed centrifuge at 9000r/min, removing impurities, finally obtaining 1 part of fibroin protein solution, and drying to obtain a berberine-fibroin protein antibacterial coating material;

s6: and (3) putting the sample into 1 part of berberine-silk fibroin mixed solution for dipping to obtain an antibacterial coating, and then controlling a spinning device by the semi-finished product through a PLC (programmable logic controller) technology to obtain a finished product.

Preferably, the processing system comprises a stirring device, a drying device, an air drying device and a spinning device, and the spinning device comprises a spinning machine and a spinning device.

Preferably, agitating unit includes the agitator tank, and the top outer wall of agitator tank has agitator motor through the fix with screw, and agitator motor's output shaft has the puddler through the coupling joint.

Preferably, drying device includes the drying cabinet, and the both sides inner wall of drying cabinet all is provided with the heating wire, and the top inner wall of drying cabinet passes through the fix with screw temperature sensor.

Preferably, the air drying device comprises an air blowing cylinder, a support is arranged on the inner wall of the air blowing cylinder, a motor is fixed on the outer wall of the support through screws, and an output shaft of the motor is connected with fan blades through a coupling.

Preferably, S5 is replaced by weighing 1 part of berberine powder, dissolving the berberine powder in deionized water to prepare a berberine solution with the concentration of 4mg/mL, adding 0.5 part of glycerol into the silk fibroin solution, uniformly mixing the berberine solution and the silk fibroin glycerol mixed solution, wherein the mass ratio of the silk fibroin to the berberine is 10:1, and drying to obtain the berberine-silk fibroin antibacterial coating material.

The invention has the beneficial effects that:

1. the invention takes chitosan fiber as a core, a collagen layer is crosslinked on the surface of the chitosan fiber through special chemical reaction, a carboxymethyl chitosan layer is crosslinked outside the collagen layer, the carboxymethyl chitosan layer, the collagen layer and the chitosan core are degraded under the action of different enzymes in a human body, and as the 3 components have different spatial positions, the sequential action sequence of the enzymes is different, the carboxymethyl chitosan and the chitosan are degraded slowly, the collagen is degraded quickly, the purpose of controlling the degradation speed is achieved by controlling the mass ratio of the three components, and the degradation speed of the suture is consistent with the wound healing speed.

1. All main components of the suture are natural compounds, compared with an absorbable suture line artificially synthesized in the market, the suture line has the advantages of high tissue compatibility, good anti-inflammatory effect, stable property and the like, the infection risk of a wound inflammation exudation period can be greatly reduced in a surgical operation, the healing effect of a granulation tissue period is assisted, the product adopts a natural traditional Chinese medicine antibacterial compound berberine as an antibacterial coating, the pollution-free silk line is ensured, the suture line also has good safety performance in non-total disinfection operations of anorectal department, general surgery and the like, the overall degradation time of the suture line is adjusted by changing the proportion of the three layers of raw materials with different degradation energy efficiency, the healing time of the wound is more fit, and the adverse prognosis effect caused by early fracture or delayed absorption is avoided.

2. The invention combines the automation technology, adopts the PLC control mode, adjusts the spinning speed, ensures that the suture structure is stable and uniform, improves the operation hand feeling, and reduces the probability of foreign body reaction of the organism.

Drawings

FIG. 1 is a schematic view of the manufacturing process and structure of the manufacturing method of absorbable surgical suture with three-layer ring structure for healing and anti-infection provided by the present invention.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.