阅读说明：本技术 一种有利于维持肠道健康的宠物食品 (Pet food beneficial to maintaining intestinal health ) 是由 李兆丰 李才明 李益文 顾正彪 班宵逢 于 2021-07-02 设计创作，主要内容包括：本发明公开了一种有利于维持肠道健康的宠物食品配方及制备方法,属于宠物食品技术领域,所述制备方法包括原料粉碎、原料混合、调制、挤压膨化、干燥、喷涂、冷却等步骤。本发明通过在宠物食品配方中加入益生元粉(环糊精),并经过宠物狗肠道微生物发酵可选择性上调益生菌(双歧杆菌和乳酸杆菌)的丰度,可预防和改善宠物便秘、腹泻、消化不良等病症的发生,有利于维持宠物的肠道健康。(The invention discloses a pet food formula beneficial to maintaining intestinal health and a preparation method thereof, belonging to the technical field of pet foods. According to the invention, the prebiotics powder (cyclodextrin) is added into the pet food formula, and the abundance of probiotics (bifidobacteria and lactobacillus) can be selectively adjusted upwards through the microbial fermentation of the pet dog intestinal tract, so that the occurrence of symptoms of constipation, diarrhea, dyspepsia and the like of the pet can be prevented and improved, and the intestinal tract health of the pet can be maintained.)

1. A pet food product for maintaining the health of the intestine, said food product comprising the following components: every 100 parts by weight of food comprises 13-18 parts of chicken, 10-14 parts of fish meat, 10-14 parts of cheese, 25-29 parts of corn flour, 20-25 parts of rice flour, 0.5-2 parts of rapeseed oil, 4-8 parts of cyclodextrin, 7-10 parts of yeast powder, 0.1-0.2 part of compound vitamin, 0.1-0.2 part of compound mineral, 0.1-0.2 part of potassium sorbate and 1.5-2 parts of edible salt; the vitamin complex comprises vitamin B₁Vitamin B₂Vitamin B₆Vitamin B₁₂Folic acid, calcium pantothenate, nicotinic acid, and vitamin AOne or more of (a); the compound mineral comprises one or more of iron, copper, zinc, manganese and selenium; the cyclodextrin comprises one or more of alpha cyclodextrin, beta cyclodextrin and gamma cyclodextrin.

2. The pet food of claim 1, wherein the cyclodextrin is a mixture of α -cyclodextrin, β -cyclodextrin, and γ -cyclodextrin in a mass ratio of 1: 1: 1, and mixing the components in a ratio of 1.

3. The pet food of claim 1 or 2, wherein the chicken meat is 16 parts, the fish meat is 13 parts, the cheese is 10 parts, the corn flour is 23 parts, the rice flour is 20 parts, the rapeseed oil is 2 parts, the cyclodextrin is 6 parts, the yeast powder is 8 parts, the vitamin complex is 0.2 part, the mineral complex is 0.1 part, the potassium sorbate is 0.2 part, and the edible salt is 1.5 parts by weight of the food.

4. The pet food of claim 1 or 2, wherein the chicken meat is 16 parts, the fish meat is 13 parts, the cheese is 10 parts, the corn flour is 21 parts, the rice flour is 20 parts, the rapeseed oil is 2 parts, the cyclodextrin is 8 parts, the yeast powder is 8 parts, the vitamin complex is 0.2 part, the mineral complex is 0.1 part, the potassium sorbate is 0.2 part, and the edible salt is 1.5 parts by weight of the food.

5. A process for preparing a pet food product according to any one of claims 1 to 4, comprising the steps of:

(1) raw material treatment: respectively crushing the raw materials of any one of claims 1 to 4 by a crusher, weighing according to a formula, and uniformly mixing by a stirrer; adding water, mixing, placing into a sealed self-sealing bag, and standing to make water be completely absorbed by the material;

(2) extruding and puffing: putting the completely prepared material in the step (1) into a feeder, and extruding and puffing;

(3) and (3) drying: drying the granular material obtained in the step (2) to reduce the moisture content of the granular material;

(4) spraying: uniformly spraying a phagostimulant on the surface of the dried granular material in the step (3) by using a grease spraying machine when the granular material is not completely cooled;

(5) and (3) cooling: cooling the sprayed granular material in the step (4) to room temperature;

(6) and (4) hermetically packaging the cooled granular material obtained in the step (5) to obtain the pet food product.

6. The method of claim 5, wherein the dried material in step (1) is ground and passed through a 14-20 mesh sieve; adding water, mixing, placing into a sealed self-sealing bag, and standing for 10-15 h; so that the water is completely absorbed by the materials, and the content of the materials is 15-25%.

7. The method as claimed in claim 5 or 6, wherein the first zone temperature of the sleeve extruded and expanded in step (2) is 65-70 ℃, the second zone temperature is 75-85 ℃, the third zone temperature is 130-160 ℃, the spindle speed is 20-35Hz, and the feeding rate is 20-30 Hz.

8. The method as claimed in any one of claims 5 to 7, wherein the drying temperature in the step (3) is 60 to 70 ℃, the drying time is 4 to 5 hours, and the moisture content of the dried material particles is 5 to 10 percent.

9. Use of a pet food product according to any one of claims 1 to 4 for modulating the abundance of bifidobacteria (bifidobacteria spp.) and lactobacilli (Lactobacillus spp.) in the gut.

10. Use of a process according to any one of claims 5 to 8 in the manufacture of a product for maintaining gut health.

Technical Field

The invention relates to a pet food beneficial to maintaining intestinal health, and belongs to the technical field of pet foods.

Background

In recent years, in order to satisfy leisure, consumption and emotional setback of residents, the proportion of pets raised by residents is increased, the market scale of the pet industry shows a trend of ultra-high speed growth, and dogs and cats are the most popular pets. Among them, pet food is the largest market segment of the pet industry. The pet food is a food which is specially provided for pets and is between human food and traditional livestock and poultry feed, and mainly has the functions of providing required nutrient substances for the pets and ensuring the growth, development and health of the pets.

The intestinal tract is used as an important organ and an immune organ for digesting and absorbing the nutrient substances of dogs and cats, contains a large number of intestinal microorganisms which exchange energy with cells of the organism and transmit information, and the health level of the intestinal tract determines the whole health state of the organism. The intestinal diseases are one of the common diseases of pets and mainly show symptoms such as constipation, diarrhea, dyspepsia and the like. The proliferation of pathogenic bacteria and the imbalance of intestinal flora caused by poor dietary habits or taking antibiotics are main causes of gastrointestinal diseases of pets.

In 2016, the international association for the science of probiotics and prebiotics (ISAPP) defined prebiotics as a substance that can be selectively utilized by the flora in the host and converted into substances beneficial to the health of the host. The reasonable intake of the prebiotics can inhibit the growth and reproduction of pathogenic bacteria in the digestive tract of animals, and is helpful for maintaining or improving the balance of intestinal flora, reducing the generation of toxins and promoting the intestinal peristalsis, thereby improving the intestinal function and improving the digestion, absorption and defecation conditions.

However, most of the existing pet foods containing prebiotics are pet foods added with probiotics, but after the probiotics are puffed at high temperature, the activity of functional components is low, and the effect is very poor, so the development of a pet food which has the probiotic effect, can prevent and improve the occurrence of symptoms of constipation, diarrhea, dyspepsia and the like of pets, and can maintain the intestinal health of pets is a hot spot.

Disclosure of Invention

The invention provides a pet food for maintaining intestinal health, which is used for preventing and improving intestinal diseases of pets caused by poor dietary habits or taking antibiotics, preventing and improving the occurrence of symptoms such as constipation, diarrhea, dyspepsia and the like of the pets and maintaining the intestinal health of the pets, and comprises the following components: every 100 parts by weight of food comprises 13-18 parts of chicken, 10-14 parts of fish meat, 10-14 parts of cheese, 25-29 parts of corn flour, 20-25 parts of rice flour, 0.5-2 parts of rapeseed oil, 4-8 parts of cyclodextrin, 7-10 parts of yeast powder, 0.1-0.2 part of vitamin complex, 0.1-0.2 part of mineral complex, 0.1-0.2 part of potassium sorbate and 1.5-2 parts of edible salt. The vitamin complex comprises vitamin B₁Vitamin B₂Vitamin B₆Vitamin B₁₂Folic acid, calcium pantothenate and tobaccoOne or more of acid and vitamin A; the compound mineral comprises one or more of iron, copper, zinc, manganese and selenium; the cyclodextrin comprises one or more of alpha cyclodextrin, beta cyclodextrin and gamma cyclodextrin.

In one embodiment of the invention, the chicken and fish meat refer to fresh chicken and fresh fish meat.

In one embodiment of the invention, the cheese is milk cheese.

In one embodiment of the invention, corn flour refers to a powdered granulate made from corn; the rice flour refers to powdery particles made of rice.

In one embodiment of the present invention, the cyclodextrin is a mixture of α -cyclodextrin, β -cyclodextrin, and γ -cyclodextrin in a mass ratio of 1: 1: 1, and mixing the components in a ratio of 1.

In one embodiment of the invention, the food product comprises the following components: every 100 parts by weight of food comprises 16 parts of chicken, 13 parts of fish, 10 parts of cheese, 25 parts of corn flour, 20 parts of rice flour, 2 parts of rapeseed oil, 4 parts of cyclodextrin, 8 parts of yeast powder, 0.2 part of compound vitamin, 0.1 part of compound mineral, 0.2 part of potassium sorbate and 1.5 parts of edible salt; the cyclodextrin is prepared by mixing alpha cyclodextrin, beta cyclodextrin and gamma cyclodextrin according to the mass ratio of 1: 1: 1 in a certain proportion; the vitamin complex is vitamin B₁Vitamin B₂Vitamin B₆Vitamin B₁₂Folic acid, calcium pantothenate, nicotinic acid and vitamin A in a mass ratio of 50: 110: 50: 1: 9: 500: 570: 10 in proportion; the composite mineral substance is prepared by mixing iron, copper, zinc, manganese and selenium according to the mass ratio of 800: 73: 1200: 50: 1, and mixing the components in a ratio of 1.

In one embodiment of the invention, the food product comprises the following components: every 100 parts by weight of food comprises 16 parts of chicken, 13 parts of fish, 10 parts of cheese, 23 parts of corn flour, 20 parts of rice flour, 2 parts of rapeseed oil, 6 parts of cyclodextrin, 8 parts of yeast powder, 0.2 part of compound vitamin, 0.1 part of compound mineral, 0.2 part of potassium sorbate and 1.5 parts of edible salt; the cyclodextrin is alpha cyclodextrin, beta cyclodextrin or gamma cyclodextrinAccording to the mass ratio of 1: 1: 1 in a certain proportion; the vitamin complex is vitamin B₁Vitamin B₂Vitamin B₆Vitamin B₁₂Folic acid, calcium pantothenate, nicotinic acid and vitamin A in a mass ratio of 50: 110: 50: 1: 9: 500: 570: 10 in proportion; the composite mineral substance is prepared by mixing iron, copper, zinc, manganese and selenium according to the mass ratio of 800: 73: 1200: 50: 1, and mixing the components in a ratio of 1.

In one embodiment of the invention, the food product comprises the following components: every 100 parts by weight of food comprises 16 parts of chicken, 13 parts of fish, 10 parts of cheese, 21 parts of corn flour, 20 parts of rice flour, 2 parts of rapeseed oil, 8 parts of cyclodextrin, 8 parts of yeast powder, 0.2 part of compound vitamin, 0.1 part of compound mineral, 0.2 part of potassium sorbate and 1.5 parts of edible salt; the cyclodextrin is prepared by mixing alpha cyclodextrin, beta cyclodextrin and gamma cyclodextrin according to the mass ratio of 1: 1: 1 in a certain proportion; the compound vitamin is vitamin B₁Vitamin B₂Vitamin B₆Vitamin B₁₂Folic acid, calcium pantothenate, nicotinic acid and vitamin A in a mass ratio of 50: 110: 50: 1: 9: 500: 570: 10 in proportion; the compound mineral substance is prepared by mixing iron, copper, zinc, manganese and selenium according to the mass ratio of 800: 73: 1200: 50: 1, and mixing the components in a ratio of 1.

The invention also provides a method for preparing the pet food, which comprises the following steps:

(1) raw material treatment: respectively crushing the dry materials in the raw materials by a crusher, sieving by a 14-20-mesh sieve, crushing the chicken, the fish and the cheese by a meat grinder, weighing all the raw materials according to the formula, and uniformly mixing by a stirrer; adding water, mixing, placing into a sealed self-sealing bag, and standing to make water be completely absorbed by the material;

(2) extruding and puffing: putting the completely prepared material in the step (1) into a feeder, and extruding and puffing;

(3) and (3) drying: drying the granular material obtained in the step (2) to reduce the moisture content of the granular material;

(4) spraying: uniformly spraying a phagostimulant on the surface of the dried granular material in the step (3) by using a grease spraying machine when the granular material is not completely cooled;

(5) and (3) cooling: and (4) cooling the sprayed granular material in the step (4) to room temperature.

(6) And (4) hermetically packaging the cooled granular material obtained in the step (5) to obtain the pet food product.

In one embodiment of the invention, the dried material obtained in the step (1) is ground and then sieved by a 14-20-mesh sieve; adding water, mixing, placing into a sealed self-sealing bag, and standing for 10-15 h; so that the water is completely absorbed by the material and the content of the material is 15-25% (w/w).

In one embodiment of the invention, the temperature of the first zone of the sleeve extruded and expanded in the step (2) is 65-70 ℃, the temperature of the second zone is 75-85 ℃, the temperature of the third zone is 130-160 ℃, the rotating speed of the main shaft is 20-35Hz, and the feeding rate is 20-30 Hz.

In one embodiment of the invention, in the step (3), the drying temperature is 60-70 ℃, the drying time is 4-5h, and the moisture content of the dried material particles is 5% -10% (w/w).

In one embodiment of the invention, in the step (4), the sprayed phagostimulant comprises one or more of rapeseed oil, soybean oil, lard, chicken oil, meat flavor, sodium glutamate and the like; the dosage is 0.1-3% (w/w).

The invention also provides the application of the pet food in adjusting the abundance of bifidobacteria (bifidobacteria spp.) and lactobacilli (Lactobacillus spp.) in the intestinal tract.

The invention also provides the application of the method in preparing a product for maintaining intestinal health.

In one embodiment of the invention, the product is a food, pharmaceutical or nutraceutical product.

Advantageous effects

1. The invention provides a prebiotic pet food containing cyclodextrin, wherein the prebiotic powder (cyclodextrin) is contained in a raw material formula in 4-8 parts by weight, so that effective colonization of probiotics in intestinal tracts can be ensured, and the prebiotic pet food has a promotion effect on improving the balance of intestinal flora and improving the intestinal tract function.

2. The invention provides a prebiotic pet food containing cyclodextrin, which can selectively up-regulate the abundance of probiotics (bifidobacteria and lactobacilli) through intestinal microbial fermentation of pets.

3. The invention provides a prebiotic pet food containing cyclodextrin, which can show the promotion effect on probiotics (bifidobacteria and lactobacillus) after being fed to a pet dog for 30 days or more.

4. The pet food provided by the invention can prevent and improve the occurrence of symptoms of constipation, diarrhea, dyspepsia and the like of pets, and is beneficial to maintaining the intestinal health of pets.

Drawings

FIG. 1: the invention is a flow chart of a preparation method of pet food.

Detailed Description

The present invention will be described in more detail with reference to the following specific examples to clearly illustrate the objects, technical solutions and advantages of the present invention.

The chicken, fish, cheese, corn flour, rice flour, rapeseed oil, edible salt and lard which are referred to in the following examples are purchased from Wuxi Jialefu supermarkets; the alpha cyclodextrin, the beta cyclodextrin and the gamma cyclodextrin are all purchased from Zhiyuan biotechnology limited of Shandong Binshon; the yeast powder is purchased from Angel Yeast GmbH; the compound vitamin is purchased from Jiangsu Bai Yao biological science and technology limited; the compound mineral is purchased from Sichuan Jilong Dake science and technology group, Inc.; potassium sorbate is available from Shanghai Michelin Biotech, Inc.

The detection methods referred to in the following examples are as follows:

the preparation method of the fecal bacteria suspension and the extraction method of RNA comprise the following steps: collecting fresh feces of 10g pet dog, suspending in 100ml of 0.1mol/L sterile phosphate physiological saline buffer solution (pH 7.2), centrifuging at 1000r/min for 5min, and collecting supernatant to obtain fecal bacteria suspension. According toProtocol for extracting fecal bacteria suspension from Soil RNA Ki kit (purchased from Beijing Solebao technologies Co., Ltd.)All RNA in the solution. The nanodrop 2000c spectrophotometer is adopted to detect the concentration of RNA, the concentration of RNA is controlled within the range of 200-1000 ng/mu L, and the ratio of the ultraviolet absorption peak value A260/A280d is within the range of 1.9-2.1.

The method for determining the abundance of bifidobacteria (Bifidobacterium spp.) and lactobacilli (Lactobacillus spp.) in excrement by qRT-PCR comprises the following steps: qRT-PCR reactions were performed according to the protocol of the SuperReal fluorescent quantitative premixed reagent enhanced (SYBR Green) kit.

Wherein, the primers of the bifidobacterium are F: TGGAAACAGRTGCTAATCCG and R: GTCCATTGTGGAAGATTCCC, and the primers of the lactobacillus are F: GGGTGGTAATGCCGGATG and R: TAAGCGATGGACTTTCACACC.

The qRT-PCR reaction program was set to pre-denaturation at 95 ℃ for 15min, denaturation at 95 ℃ for 10sec, annealing/extension at 60 ℃ for 32sec, and 40 cycles of denaturation and annealing/extension were performed. Beta-actin is used as internal reference, 2 is adopted^-ΔΔThe Ct method calculates the relative abundance of bifidobacteria and lactobacilli.

Example 1: preparation process of pet food

The present embodiment provides a process for preparing a pet food beneficial to maintaining intestinal health (as shown in fig. 1), which comprises the following steps:

(1) crushing raw materials: respectively crushing dry materials in the raw materials by a crusher, and sieving the crushed dry materials by a 14-20-mesh sieve, wherein the dry materials refer to: corn flour, rice flour, cyclodextrin, yeast powder, compound vitamin, compound mineral substance, potassium sorbate and edible salt; mincing chicken, fish and cheese by a meat mincer, weighing all the raw materials according to a formula, uniformly mixing the raw materials by a stirrer, adding water, blending, putting the mixture into a sealed self-sealing bag, standing for 15 hours to ensure that the water is completely absorbed by the materials, wherein the water content of the blended materials is 20%;

(2) extruding and puffing: putting the completely prepared material in the step (1) into a feeder, and extruding and puffing; the first zone temperature of the extruded and puffed sleeve is 65 ℃, the second zone temperature is 75 ℃, the third zone temperature is 150 ℃, the main shaft rotating speed is 25Hz, and the feeding speed is 25Hz, so as to obtain a granular material;

(3) and (3) drying: drying the granular material obtained in the step (2) to reduce the moisture content of the granular material; the drying temperature is 65 ℃, the drying time is 4 hours, and the moisture content of the dried material particles is 8% (w/w);

(4) spraying: when the dried granular material in the step (3) is cooled to 50 ℃, a grease spraying machine is used for uniformly spraying a phagostimulant on the surface of the granular material, the amount of the phagostimulant sprayed on the surface of the granular material is 1.5% (namely 1.5g of the phagostimulant is sprayed on each 100g of the surface of the granular material), the phagostimulant can be one or more of rapeseed oil, soybean oil, lard, chicken oil, meat flavor, sodium glutamate and the like, the effects of the phagostimulant are the same, and the lard is used as the phagostimulant in the embodiment;

(5) and (3) cooling: and (4) cooling the granular material sprayed with the phagostimulant in the step (4) to room temperature.

(6) And (4) hermetically packaging the cooled granular material obtained in the step (5) to obtain the pet food product.

Example 2: preparation of pet food with different formulations

According to the preparation process of the example 1, different raw material formulas are adjusted to prepare the pet food, and the specific formula is as follows:

formula 1:

every 100 parts by weight of food comprises 16 parts of chicken, 13 parts of fish, 10 parts of cheese, 25 parts of corn flour, 20 parts of rice flour, 2 parts of rapeseed oil, 4 parts of cyclodextrin, 8 parts of yeast powder, 0.2 part of compound vitamin, 0.1 part of compound mineral, 0.2 part of potassium sorbate and 1.5 parts of edible salt; pet food 1 was prepared.

Wherein the cyclodextrin is prepared by mixing alpha cyclodextrin, beta cyclodextrin and gamma cyclodextrin according to the mass ratio of 1: 1: 1 in a certain proportion;

the compound vitamin is vitamin B₁Vitamin B₂Vitamin B₆Vitamin B₁₂Folic acid, calcium pantothenate, nicotinic acid and vitamin A in a mass ratio of 50: 110: 50: 1: 9: 500: 570: 10 in proportion;

the compound mineral substance is prepared by mixing iron, copper, zinc, manganese and selenium according to the mass ratio of 800: 73: 1200: 50: 1, and mixing the components in a ratio of 1.

And (2) formula:

every 100 parts by weight of food comprises 16 parts of chicken, 13 parts of fish, 10 parts of cheese, 23 parts of corn flour, 20 parts of rice flour, 2 parts of rapeseed oil, 6 parts of cyclodextrin, 8 parts of yeast powder, 0.2 part of compound vitamin, 0.1 part of compound mineral, 0.2 part of potassium sorbate and 1.5 parts of edible salt; pet food 2 is prepared.

Wherein the cyclodextrin is prepared by mixing alpha cyclodextrin, beta cyclodextrin and gamma cyclodextrin according to the mass ratio of 1: 1: 1 in a certain proportion;

the compound vitamin is vitamin B₁Vitamin B₂Vitamin B₆Vitamin B₁₂Folic acid, calcium pantothenate, nicotinic acid and vitamin A in a mass ratio of 50: 110: 50: 1: 9: 500: 570: 10 in proportion;

the compound mineral substance is prepared by mixing iron, copper, zinc, manganese and selenium according to the mass ratio of 800: 73: 1200: 50: 1, and mixing the components in a ratio of 1.

And (3) formula:

every 100 parts by weight of food comprises 16 parts of chicken, 13 parts of fish, 10 parts of cheese, 21 parts of corn flour, 20 parts of rice flour, 2 parts of rapeseed oil, 8 parts of cyclodextrin, 8 parts of yeast powder, 0.2 part of compound vitamin, 0.1 part of compound mineral, 0.2 part of potassium sorbate and 1.5 parts of edible salt; pet food 3 is prepared.

Wherein, the cyclodextrin is prepared by mixing alpha cyclodextrin, beta cyclodextrin and gamma cyclodextrin according to the weight ratio of 1: 1: 1 in a certain proportion;

the compound vitamin is vitamin B₁Vitamin B₂Vitamin B₆Vitamin B₁₂Folic acid, calcium pantothenate, nicotinic acid and vitamin A in a mass ratio of 50: 110: 50: 1: 9: 500: 570: 10 in proportion;

the compound mineral substance is prepared by mixing iron, copper, zinc, manganese and selenium according to the mass ratio of 800: 73: 1200: 50: 1, and mixing the components in a ratio of 1.

Comparative example

Formulation 4

The specific formula is the same as the formula 1, and is different from the formula 1 in that cyclodextrin is not added, and the specific formula is as follows:

every 100 parts by weight of food comprises 16 parts of chicken, 13 parts of fish, 10 parts of cheese, 29 parts of corn flour, 20 parts of rice flour, 2 parts of rapeseed oil, 8 parts of yeast powder, 0.2 part of compound vitamin, 0.1 part of compound mineral substance, 0.2 part of potassium sorbate and 1.5 parts of edible salt; pet food 4 is prepared.

Wherein the vitamin complex is vitamin B₁Vitamin B₂Vitamin B₆Vitamin B₁₂Folic acid, calcium pantothenate, nicotinic acid and vitamin A in a mass ratio of 50: 110: 50: 1: 9: 500: 570: 10 in proportion;

the compound mineral substance is prepared by mixing iron, copper, zinc, manganese and selenium according to the mass ratio of 800: 73: 1200: 50: 1, and mixing the components in a ratio of 1.

Formulation 5

The specific formula is the same as the formula 1, and the difference is that the cyclodextrin is alpha cyclodextrin, which is specifically as follows:

every 100 parts by weight of food comprises 16 parts of chicken, 13 parts of fish, 10 parts of cheese, 25 parts of corn flour, 20 parts of rice flour, 2 parts of rapeseed oil, 4 parts of cyclodextrin, 8 parts of yeast powder, 0.2 part of compound vitamin, 0.1 part of compound mineral, 0.2 part of potassium sorbate and 1.5 parts of edible salt; pet food 5 is prepared.

Wherein the cyclodextrin is alpha cyclodextrin;

the compound vitamin is vitamin B₁Vitamin B₂Vitamin B₆Vitamin B₁₂Folic acid, calcium pantothenate, nicotinic acid and vitamin A in a mass ratio of 50: 110: 50: 1: 9: 500: 570: 10 in proportion;

the compound mineral substance is prepared by mixing iron, copper, zinc, manganese and selenium according to the mass ratio of 800: 73: 1200: 50: 1, and mixing the components in a ratio of 1.

Formulation 6

The specific formula is the same as the formula 1, and the difference is that the cyclodextrin is beta cyclodextrin, which is specifically as follows:

every 100 parts by weight of food comprises 16 parts of chicken, 13 parts of fish, 10 parts of cheese, 25 parts of corn flour, 20 parts of rice flour, 2 parts of rapeseed oil, 4 parts of cyclodextrin, 8 parts of yeast powder, 0.2 part of compound vitamin, 0.1 part of compound mineral, 0.2 part of potassium sorbate and 1.5 parts of edible salt; pet food 6 is prepared.

Wherein the cyclodextrin is beta cyclodextrin;

the compound vitamin is vitamin B₁Vitamin B₂Vitamin B₆Vitamin B₁₂Folic acid, calcium pantothenate, nicotinic acid and vitamin A in a mass ratio of 50: 110: 50: 1: 9: 500: 570: 10 in proportion;

the compound mineral substance is prepared by mixing iron, copper, zinc, manganese and selenium according to the mass ratio of 800: 73: 1200: 50: 1, and mixing the components in a ratio of 1.

And (3) formula 7:

the specific formula is the same as the formula 1, and the difference is that the cyclodextrin is gamma cyclodextrin, which is specifically as follows:

every 100 parts by weight of food comprises 16 parts of chicken, 13 parts of fish, 10 parts of cheese, 25 parts of corn flour, 20 parts of rice flour, 2 parts of rapeseed oil, 4 parts of cyclodextrin, 8 parts of yeast powder, 0.2 part of compound vitamin, 0.1 part of compound mineral, 0.2 part of potassium sorbate and 1.5 parts of edible salt; pet food 7 is prepared.

Wherein the cyclodextrin is gamma cyclodextrin;

the compound vitamin is vitamin B₁Vitamin B₂Vitamin B₆Vitamin B₁₂Folic acid, calcium pantothenate, nicotinic acid and vitamin A in a mass ratio of 50: 110: 50: 1: 9: 500: 570: 10 in proportion;

the compound mineral substance is prepared by mixing iron, copper, zinc, manganese and selenium according to the mass ratio of 800: 73: 1200: 50: 1, and mixing the components in a ratio of 1.

Example 3: effect of different formulations of Pet food on the abundance of Probiotics in the Pet gut

The pet food 1-7 prepared in example 2 was fed to pet dogs, and the abundance of probiotics (bifidobacteria and lactobacilli) in feces of pet dogs before and after feeding was determined.

The method comprises the following specific steps:

the specific embodiment of the pet dog feeding experiment is as follows:

36 pet dogs were selected, of which 12 dogs each were a doll (small dog), a puppy (medium dog), and a golden hair (large dog). Each breed of pet dog was randomly divided into 4 groups of 3 dogs, each group was fed with pet foods 1 to 7 prepared in example 2 (only pet foods and drinking water prepared according to the present invention were fed during the experiment), and 12 experimental groups were counted, 3 dogs in each group were paralleled (no control group was provided separately because of the large individual difference in intestinal flora), and the food intake was 15 g/kg/d.

Feces of pet dogs were collected on 0, 30 and 60 days of feeding, respectively, and abundances of Bifidobacterium (Bifidobacterium spp.) and Lactobacillus (Lactobacillus spp.) in intestinal tracts of different dog types after eating pet foods of different formulas were calculated, respectively, with the results shown in tables 1-2:

table 1: abundance of bifidobacteria and lactobacilli in intestinal tracts of different dogs eating pet foods with formulas 1-4

Table 2: abundance of bifidobacteria and lactobacilli in intestinal tract following consumption of pet food according to formulas 5, 6, and 7 by different canine types

As can be seen from tables 1 and 2, the relative abundances of probiotics (bifidobacterium and lactobacillus) in feces of a doll (a small dog), a dog (a medium dog) and a golden hair (a large dog) are regulated by using pet food 1, pet food 2 and pet food 3 (prebiotic powder is prepared by mixing alpha cyclodextrin, beta cyclodextrin and gamma cyclodextrin according to the ratio of 1: 1: 1) prepared by using the method provided by the invention, compared with pet food 4 (no prebiotic powder is added), and the regulation effect is superior to that of pet food 5 (prebiotic powder is only alpha cyclodextrin), pet food 6 (prebiotic powder is only beta cyclodextrin) and pet food 7 (prebiotic powder is only gamma cyclodextrin).

The analysis data shows that the pet food 2 and 3 has the most obvious regulating effect on the bifidobacteria and the lactobacilli, the bifidobacteria respectively increase 3.80, 1.92 and 2.32log cfu/g on day 60 when the pet food 2 is fed with the doll, the favich and the golden hair, the lactobacilli respectively increase 1.77, 0.38 and 1.76log cfu/g, the bifidobacteria respectively increase 2.14, 2.30 and 2.35log cfu/g on day 60 when the pet food 3 is fed with the doll, the favich and the golden hair, and the lactobacilli respectively increase 1.59, 1.02 and 0.99log cfu/g. In addition, pet food 4 prepared without addition of prebiotic powder (cyclodextrin) had little intestinal flora modulating ability, and pet foods 5, 6, and 7 prepared with addition of alpha cyclodextrin, beta cyclodextrin, and gamma cyclodextrin alone could up-regulate the probiotic abundance in feces, but the up-regulation ability was lower than pet foods 2 and 3. The situation that the addition of cyclodextrin in a pet food formula can promote effective colonization of probiotics in the intestinal tract of a pet dog is indirectly reflected, and the compounding effect of alpha cyclodextrin, beta cyclodextrin and gamma cyclodextrin is superior to that of the addition of one type of cyclodextrin, so that the occurrence of symptoms of constipation, diarrhea, dyspepsia and the like of pets can be prevented and improved, and the intestinal tract health of the pets is favorably maintained.

Although the present invention has been described with reference to the preferred embodiments, it should be understood that various changes and modifications can be made therein by those skilled in the art without departing from the spirit and scope of the invention as defined in the appended claims.