阅读说明：本技术 具有肠道调节排毒清毒的乳果糖、微生物组合物及其用途 (Lactulose and microbial composition with functions of regulating intestinal tracts, expelling toxin and clearing toxin and application thereof ) 是由 郑涵 席宾 刘春颖 于 2021-07-09 设计创作，主要内容包括：本发明涉及保健食品技术领域,具体涉及一种具有肠道调节排毒清毒的乳果糖、微生物组合物及其用途,所述组合物,包括如下组分：乳果糖、低聚果糖、水苏糖、魔芋多糖、大枣提取物和益生菌,具有排毒效果好、无副作用的优点。(The invention relates to the technical field of health-care food, in particular to a lactulose and microorganism composition with functions of regulating intestinal tracts, expelling toxin and clearing away toxin and an application thereof, wherein the composition comprises the following components: lactulose, fructo-oligosaccharide, stachyose, konjac polysaccharide, Chinese date extract and probiotics have the advantages of good toxin expelling effect and no side effect.)

1. A composition comprising the following components: lactulose, fructo-oligosaccharide, stachyose, konjac polysaccharide, Chinese date extract and probiotics.

2. The composition according to claim 1, which comprises the following components in parts by weight: 20-30 parts of lactulose, 4-7 parts of fructo-oligosaccharide, 5-10 parts of stachyose, 6-15 parts of konjac polysaccharide, 4-9 parts of Chinese date extract and 3-7 parts of probiotics.

3. The composition of claim 1, wherein the probiotic is at least one of lactobacillus acidophilus and bifidobacterium.

4. The composition of claim 1, wherein the fructooligosaccharide is at least one of sucrose trisaccharide and sucrose tetrasaccharide.

5. The composition of claim 1, wherein the fructo-oligosaccharide comprises, in parts by weight, 2-4 parts of sucrose trisaccharide and 2-3 parts of sucrose tetrasaccharide.

6. The composition as claimed in claim 1, wherein the process for preparing the jujube extract comprises the steps of:

(1) extracting dried and ripe fructus Jujubae with 50-70% ethanol, and filtering to obtain extractive solution;

(2) recovering ethanol from the extractive solution, extracting with ethyl acetate, recovering ethyl acetate from the extracted part, dissolving with water, passing through macroporous adsorbent resin, eluting with solvent A, and collecting eluate;

(3) concentrating the eluate, and drying.

7. The composition as claimed in claim 6, wherein the solvent A is a mixed solvent of 50-70% ethanol and propylene glycol, and the volume ratio of the two is 2-4: 1.

8. The composition of claim 6, wherein the macroporous adsorbent resin is one of D101 and H-60.

9. A formulation comprising a composition according to any one of claims 1 to 8.

10. Use of the composition of any one of claims 1 to 8 or the preparation of claim 9 for preparing a health product with functions of intestinal regulation, toxin expelling and toxin clearing.

Technical Field

The invention relates to the technical field of health-care food, in particular to a lactulose and microorganism composition with functions of regulating intestinal tracts, expelling toxin and clearing toxin and application thereof.

Background

Human body toxin refers to the excess waste in the body. The sources of it are mainly two: the metabolic waste generated after digestion and absorption of food is retained; various contaminants from the environment are deposited in the body. Toxins are substances that interfere with normal physiological activities and disrupt body function. The inherent toxin is free radical, cholesterol, fat, uric acid, lactic acid, water poison and extravasated blood, etc. Exotic toxins such as pesticide residues in vegetables, automobile exhaust, industrial waste gases, chemicals, radiation, preservatives in food, overproof heavy metals in cosmetics, pathogenic microorganisms, etc.

From the perspective of traditional Chinese medicine, "poison" is divided into interior and exterior. The external toxicity is a substance harmful to human body, such as toxic inorganic substances like lead and mercury, and includes a great influence of external environment on human body. Endogenous toxins are often caused by weakness of the spleen and stomach, bad mood and bad lifestyle habits.

Normal persons do not require human intervention to "detoxify" because every healthy body has the ability to eliminate various toxins. Liver, kidney, intestine, skin, etc., which are metabolic organs of the human body, i.e., toxin expelling organs, actively expel or enzymatically hydrolyze toxic substances that have entered the human body. For example, the liver, the largest detoxifying organ, can decompose toxic substances in the body through a series of biochemical reactions. Excrete, sweat, breath, cough, vomiting, etc., and also excrete metabolic waste.

The traditional Chinese medicine considers that if the healthy qi of the human body is vigorous and the resistance is strong, the attack of various toxins can be resisted, and the toxins are naturally discharged out of the human body. Indeed, the human body has a function of eliminating toxins from the body, and the organs work in order to maintain the various functions of the body.

However, factors such as wrong eating habits, bad lifestyle, environmental pollution, mood and stress can cause biochemical imbalance of the body and affect the emission of toxins. Biochemical imbalances are systemic pathological phenomena throughout the body, and thus any effective therapy must be a holistic therapy, which includes mainly vegetarian detoxification therapy, nutritional therapy, herbal therapy, health meals, and aerobic exercise, among others.

Studies have shown that foods that help the body to excrete a variety of "toxins" are: (1) green leaf vegetables. The green leaf vegetables are alkaline, can neutralize excessive acidic substances generated in sugar, meat, eggs and metabolism in diet, and keep body fluid weakly alkaline, thereby removing toxic substances in blood. (2) Coarse food grains. Frequent eating of coarse food grains such as sweet potatoes, corns, buckwheat and the like is helpful for keeping the defecate smooth, so that toxic substances in the body can not stagnate in the intestinal tract for a long time. (3) Fruit or fruit juice. Can be used for eating fructus Citri Limoniae, fructus Citri Tangerinae, fructus Citri Grandis, fructus Vitis Viniferae, sugarcane juice, mume fructus, fructus Mali Pumilae, fructus Lycopersici Esculenti, etc. Although the fruit tastes sour, the fruit can be changed into alkaline in the metabolism process in vivo and can keep the blood alkaline. In particular, they are capable of "lysing" toxins that accumulate in the cells and are ultimately excreted through the excretory system. (4) Green tea. Green tea contains many detoxifying factors that are easily bound to toxic substances in the blood and accelerate the excretion from the urine. (5) A wine is provided. The wine is beneficial to heart health. It is rich in citric acid and also belongs to alkaline beverages, which are not possessed by many alcoholic beverages. (6) Kelp and laver. They contain a large amount of gum. Can relieve constipation and promote the excretion of radioactive toxin from human body. (7) Black fungus. The colloid in the black fungus is helpful for adsorbing and aggregating dust impurities remained in the digestive system of a human body and discharging the dust impurities out of the human body, and cleaning the stomach and intestine. (8) Fermented soya beans. Researches show that eating the fermented soya beans has the effects of helping digestion, enhancing the brain, improving the detoxifying capability of the liver and the like.

However, the effect of detoxification and detoxification through single food therapy is limited, so that the detoxification of intestinal tracts is regulated in the prior art by adopting a mode of combining a plurality of substances.

The Chinese patent application CN112273621A discloses a soup for clearing away toxin and a preparation method thereof, and the soup comprises the following raw materials by weight: 30-60g of red peony root, 30-60g of white peony root, 10-30g of dwarf lilyturf tuber, 10-30g of rehmannia root, 10g of bitter orange, 10-30g of konjaku flour, 10-60g of mung bean and 10-30g of green olive can have the auxiliary treatment effect on cardiovascular and cerebrovascular diseases, hypertension, hyperlipidemia, hyperuricemia and hypoimmunity, and can achieve the effects of expelling toxin, maintaining beauty and keeping young. However, the toxin expelling effect of the toxin expelling soup is slow, and various substances are adopted, so that the toxin expelling mechanism is not clear, and the curative effect is unstable.

Therefore, it is necessary to develop lactulose, microbial composition and its use with functions of regulating intestinal tract, expelling toxin and clearing away toxic materials.

Disclosure of Invention

The invention aims to overcome the defects of the prior art and provide lactulose, microbial composition with good toxin expelling effect and no side effect and application thereof.

The invention is realized by the following technical scheme:

a composition comprising the following components: lactulose, fructo-oligosaccharide, stachyose, konjac polysaccharide, Chinese date extract and probiotics.

Preferably, the composition comprises the following components in parts by weight: 20-30 parts of lactulose, 4-7 parts of fructo-oligosaccharide, 5-10 parts of stachyose, 6-15 parts of konjac polysaccharide, 4-9 parts of Chinese date extract and 3-7 parts of probiotics.

Preferably, the probiotic is at least one of lactobacillus acidophilus and bifidobacterium.

Preferably, the fructooligosaccharide is at least one of trisaccharide and tetrasaccharide.

Preferably, the fructo-oligosaccharide comprises 2-4 parts by weight of sucrose trisaccharide and 2-3 parts by weight of sucrose tetrasaccharide.

Preferably, the preparation process of the jujube extract comprises the following steps:

(1) extracting dried and ripe fructus Jujubae with 50-70% ethanol, and filtering to obtain extractive solution;

(2) recovering ethanol from the extractive solution, extracting with ethyl acetate, recovering ethyl acetate from the extracted part, dissolving with water, passing through macroporous adsorbent resin, eluting with solvent A, and collecting eluate;

(3) concentrating the eluate, and drying.

More preferably, step (1) comprises the steps of:

extracting dried and mature fructus Jujubae with 5-10 times of 50-70% ethanol for 1-3 times at 50-60 deg.C for 1-2 hr, and mixing extractive solutions.

More preferably, the solvent A is a mixed solvent of 50-70% ethanol and propylene glycol, and the volume ratio of the two is 2-4: 1.

More preferably, the macroporous adsorbent resin is one of D101 and H-60.

More preferably, the step (2) comprises the steps of:

recovering ethanol from the extractive solution, extracting with ethyl acetate, recovering ethyl acetate from the extracted part, dissolving with 1-3 times of water (based on the mass of the recovered ethyl acetate), passing through macroporous adsorbent resin (one of D101 and H-60), eluting with solvent A (mixed solvent of 50-70% ethanol and propylene glycol at volume ratio of 2-4:1), and collecting eluate.

More preferably, the preparation process of the jujube extract comprises the following steps:

(1) extracting dried and mature fructus Jujubae with 5-10 times of 50-70% ethanol for 1-3 times at 50-60 deg.C for 1-2 hr, and mixing extractive solutions;

(2) recovering ethanol from the extractive solution, extracting with ethyl acetate, recovering ethyl acetate from the extracted part, dissolving with 1-3 times of water, passing through macroporous adsorbent resin (one of D101 and H-60), eluting with solvent A (mixed solvent of 50-70% ethanol and propylene glycol at volume ratio of 2-4:1), and collecting eluate;

(3) concentrating the eluate, and drying.

The invention also relates to a preparation comprising the composition.

The invention also relates to application of the composition or the preparation in preparing health products with functions of regulating intestinal tracts, expelling toxin and clearing toxin.

The invention has the beneficial effects that:

the composition of the composition is optimized, different saccharides, the Chinese date extract and the probiotics are used in a composite mode, the synergistic effect is obvious, the intestinal tract adjusting function and the toxin expelling and clearing effects are excellent, and the composition is free of toxic and side effects through testing. Particularly, the lactulose, the stachyose and the konjac polysaccharide have remarkable synergistic effect, and the ratio of the lactulose, the stachyose and the konjac polysaccharide is optimized, so that various effects of the product are remarkably improved.

The invention optimizes the composition of probiotics, is beneficial to assisting in regulating intestinal functions, and further improves the effects of expelling toxin and clearing toxin.

The invention optimizes the preparation process of the Chinese date extract, the prepared Chinese date extract has more obvious synergistic effect with other components, and the intestinal tract regulating function and the effects of expelling toxin and clearing toxin are obviously improved.

Detailed Description

The invention will be further described with reference to specific embodiments, and the advantages and features of the invention will become apparent as the description proceeds. These examples are illustrative only and do not limit the scope of the present invention in any way. It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention, and that such changes and modifications may be made without departing from the spirit and scope of the invention.

The konjac polysaccharides in the examples and comparative examples of the present invention were purchased from Saiko Olympic Biotech Ltd, having a product number QADT-12. Lactobacillus acidophilus powder was purchased from West Anxin Biotech limited, cat # XL 180614.

Example 1

The composition comprises the following components in parts by weight: 20 parts of lactulose, 2 parts of sucrose trisaccharide, 2 parts of sucrose tetrasaccharide, 5 parts of stachyose, 6 parts of konjac polysaccharide, 4 parts of Chinese date extract and 3 parts of lactobacillus acidophilus powder.

The preparation process of the Chinese date extract comprises the following steps:

(1) extracting dried and mature fructus Jujubae with 5 times of 50% ethanol for 3 times at 50 deg.C for 1 hr, filtering, and mixing extractive solutions;

(2) recovering ethanol from the extractive solution, extracting with ethyl acetate, recovering ethyl acetate from the extracted part, dissolving with 1 times of water, passing through D101 macroporous adsorbent resin, eluting with solvent A (the solvent A is a mixed solvent of 50% ethanol and propylene glycol, and the volume ratio of the two is 2:1), and collecting eluate;

(3) concentrating the eluate, and drying.

Example 2

The composition comprises the following components in parts by weight: 30 parts of lactulose, 4 parts of sucrose trisaccharide, 3 parts of sucrose tetrasaccharide, 10 parts of stachyose, 15 parts of konjac polysaccharide, 9 parts of Chinese date extract and 7 parts of lactobacillus acidophilus powder.

The preparation process of the Chinese date extract comprises the following steps:

(1) extracting dried and mature fructus Jujubae with 10 times of 70% ethanol for 1 time for 2 hr at 60 deg.C, and filtering to obtain extractive solution;

(2) recovering ethanol from the extractive solution, extracting with ethyl acetate, recovering ethyl acetate from the extracted part, dissolving with 3 times of water, passing through D101 macroporous adsorbent resin, eluting with solvent A (70% ethanol and propylene glycol mixed solvent at volume ratio of 4:1), and collecting eluate;

(3) concentrating the eluate, and drying.

Example 3

The composition comprises the following components in parts by weight: 25 parts of lactulose, 3 parts of sucrose trisaccharide, 3 parts of sucrose tetrasaccharide, 8 parts of stachyose, 12 parts of konjac polysaccharide, 6 parts of Chinese date extract and 5 parts of lactobacillus acidophilus powder.

The preparation process of the Chinese date extract comprises the following steps:

(1) extracting dried and mature fructus Jujubae with 8 times of 60% ethanol for 2 times at 55 deg.C for 1.5 hr, filtering, and mixing extractive solutions;

(2) recovering ethanol from the extractive solution, extracting with ethyl acetate, recovering ethyl acetate from the extracted part, dissolving with 2 times of water, passing through D101 macroporous adsorbent resin, eluting with solvent A (the solvent A is a mixed solvent of 60% ethanol and propylene glycol, and the volume ratio of the two is 3:1), and collecting eluate;

(3) concentrating the eluate, and drying.

Comparative example 1

The difference from the embodiment 3 is that 25 parts of lactulose are replaced by 10 parts of stachyose and 15 parts of konjak polysaccharide, and the other conditions are the same, and the specific conditions are as follows:

the composition comprises the following components in parts by weight: 3 parts of sucrose trisaccharide, 3 parts of sucrose tetrasaccharide, 18 parts of stachyose, 27 parts of konjak polysaccharide, 6 parts of Chinese date extract and 5 parts of lactobacillus acidophilus powder.

Comparative example 2

The difference from the embodiment 3 is that 8 parts of stachyose and 12 parts of konjak polysaccharide are replaced by 20 parts of lactulose, and the other conditions are the same, and the specific conditions are as follows:

the composition comprises the following components in parts by weight: 45 parts of lactulose, 3 parts of trisaccharide, 3 parts of tetrasaccharide, 6 parts of Chinese date extract and 5 parts of lactobacillus acidophilus powder.

Comparative example 3

The only difference from example 3 is that no jujube extract was added, and the other conditions were the same.

Comparative example 4

The difference from the example 3 is only that the composition of the solvent A is different, the solvent A is a mixed solvent of 60% ethanol and propylene glycol, the volume ratio of the two is 1:2, and the rest conditions are the same.

Test example 1

Regulating intestinal function

According to a defecation function test method in health food test and evaluation technical specifications, a compound diphenoxylate is adopted to establish a mouse constipation model, 10 mice in each group are used, and the administration time of a test sample is 7 days.

The specific method for measuring the index comprises the following steps: after administering the compound diphenoxylate for 0.5h, the mice in the model control group are perfused with ink (containing 5% of activated carbon powder and 10% of gum arabic), and the mice in the administration group are administered with ink containing the tested medicine, and the animals are all fed in a single cage and normally drink water. The dosage of the test drug is 270mg/kg BW per day.

Starting from the ink filling, the black excrement discharging time of the first grains of each animal and the number and weight of the black excrement discharging grains within 5 hours are measured.

The results are shown in Table 1.

TABLE 1

Test sample	First particle black stool discharging time (h)	Number of black stool particles discharged within 5h	Weight (g) of black stool discharged in 5h
				Model control group	3.96±1.04a	7.8±2.4a	0.42±0.05a
Example 1	2.17±0.42b	11.2±3.9b	0.67±0.07b
				Example 2	2.14±0.38b	11.5±4.1b	0.68±0.11b
Example 3	2.08±0.71b	11.8±4.7b	0.71±0.15b
				Comparative example 1	2.53±0.94c	9.2±3.2c	0.55±0.06c
Comparative example 2	2.46±0.75c	9.9±3.5c	0.59±0.09c
				Comparative example 3	2.40±0.67c	9.6±2.8c	0.57±0.13c
Comparative example 4	2.21±0.83c	10.7±3.3c	0.61±0.18c

a. b, c different letters indicate that there is a significant difference between the groups.

The above detailed description is specific to one possible embodiment of the present invention, and the embodiment is not intended to limit the scope of the present invention, and all equivalent implementations or modifications without departing from the scope of the present invention should be included in the technical scope of the present invention.