阅读说明：本技术 一种含海茴香愈伤组织培养物滤液的修护蓝光损伤组合物及应用 (Repair blue light injury composition containing hypecoum vulgare callus culture filtrate and application ) 是由 吕平平 李传茂 张伟杰 张楚标 于 2020-05-20 设计创作，主要内容包括：本发明属于化妆品领域,具体涉及一种含海茴香愈伤组织培养物滤液的修护蓝光损伤组合物及应用。本发明的组合物包括海茴香愈伤组织培养物滤液、乳酸杆菌/北美圣草发酵产物提取物、杉叶蕨藻提取物、紫苑提取物、表棓儿茶酚棓酸葡糖苷,几种组分协同增效,能最大程度减少蓝光照射下产生的自由基、修护蓝光引起的损伤；同时也能解决由蓝光辐射引起的皮肤老化的问题。(The invention belongs to the field of cosmetics, and particularly relates to a blue light injury repairing composition containing hypecoum vulgare callus culture filtrate and application thereof. The composition comprises hypsizygus marmoreus callus culture filtrate, lactobacillus/eriodictyon fermentation product extract, codium meridionatum extract, aster extract and epigallocatechin gallate glucoside, and the components are synergistic, so that free radicals generated under blue light irradiation can be reduced to the maximum extent, and damage caused by blue light can be repaired; and simultaneously, the problem of skin aging caused by blue light radiation can be solved.)

1. A composition, comprising: hypecoum culture filtrate, lactobacillus/eriodictyon fermentation product extract, Coptoterus cuneatus extract, Aster extract, epigallocatechin gallate glucoside.

2. The composition of claim 1, comprising the following in parts by weight:

preferably, the composition comprises the following substances in parts by weight:

3. the composition of claim 1, wherein the hypecoum vulgare callus culture filtrate is obtained by:

1) slicing the pimpinella anisum root, putting the sliced pimpinella anisum root into a basic culture medium, and adding sucrose, agar and dichlorophenoxyacetic acid for culturing to obtain callus;

2) placing the callus prepared in the step 1) in a basic culture medium, and adding sucrose, dichlorophenoxyacetic acid and kinetin for culturing to obtain the suspension cells.

4. The composition of claim 3, wherein in the step 1), the mass fraction of sucrose is 1.5-4.5%, the addition amount of agar is 7-8.5 g/L, and the addition amount of dichlorophenoxyacetic acid is 0.5-1.5 mg/L; preferably, step 1) culturing for 20-30 days; further preferably for 25 days;

preferably, in the step 2), in the basic culture medium, the adding mass fraction of sucrose is 1.5-4.5%, the adding amount of dichlorophenoxyacetic acid is 0.2-1.2 mg/L, and the adding amount of kinetin is 0.01-0.5 mg/L; preferably, the culturing in step 2) is carried out for 20 to 30 days; further preferably, the culture is carried out for 25 days.

5. The composition of claim 3, further comprising the step of filtering the suspension of cells produced and collecting the filtrate.

6. Use of the composition of claim 1 for the preparation of a skin care product having the efficacy of repairing blue light damage; preferably, the skin care product comprises one or more of eye cream, face cream, essence and toner.

7. A cosmetic comprising the composition of any one of claims 1 to 6; preferably, the composition is added in an amount of 3-20%, preferably 6-16%.

8. The cream of claim 6, comprising the following ingredients in weight percent:

preferably, the addition amount of the composition is 6-16%; more preferably 10%.

9. A method of making a cream containing a remedial blue light damage composition according to claim 8, comprising the steps of:

(1) putting the phase A into a water phase pot, uniformly stirring, heating and stirring to 80-85 ℃, and preserving heat for 8-20 min;

(2) putting the phase B into an oil phase pot, heating and stirring to 80-85 ℃, and stirring;

(3) and pumping the materials in the water phase pot into an emulsifying pot. Pumping the materials in the oil phase pot into an emulsifying pot, stirring, vacuumizing, homogenizing, and stirring for 10-25 min;

(4) cooling to 40-45 deg.C, adding phase C, and stirring.

Technical Field

The invention belongs to the field of cosmetics, and particularly relates to a blue light injury repairing composition containing hypecoum vulgare callus culture filtrate and application thereof.

Background

Blue Light is Visible Light with a wavelength of 400-500nm, is the shortest Visible Light, and is also called High Energy Visible Light (HEVL), and has a strong penetrating power and can penetrate through the epidermis layer and the dermis layer of the skin. The blue light mainly comes from natural light, LED lamps, and electronic equipment such as mobile phones and computers. Research shows that blue light is related to sleep disorder, can cause eye fatigue and even cause visual disturbance, and in 2005, Bernard reports the damage of blue light to skin for the first time, generates free radicals and causes skin aging.

Damage caused by blue light, weakened free radical scavenging ability, and skin aging have not been suitable skin care products. However, with the change of living habits of people, people are inevitably subjected to a large amount of blue light irradiation every day after being exposed to electronic products for more and more time, and the damage of the skin caused by the blue light is more and more valued by people.

Disclosure of Invention

In one aspect, the invention provides a composition comprising: crithmum maritimum callus culture filtrate (Crithmum maritimum culture broth), Lactobacillus/Halloween (Eriodictynencalcium) fermentation product extract, Pteridium Giganteum (Caulerpa Taxifolia) extract, Asteria (Astericularis) extract, and epigallocatechin gallate glucoside (epigallocatechin gallate glucoside).

The sea fennel is an aromatic plant of Umbelliferae growing on coast, and has antioxidant activity higher than that of alpha-tocopherol and BHT at all concentrations, and can inhibit linoleic acid peroxidation. Simultaneously, the composition can inhibit the activity of tyrosinase and has whitening effect.

Plant stem cells are undifferentiated cells, the active components of which have intact biological functions, each undifferentiated cell containing all the components of the plant and being the source of new cell growth or replacement for a particular tissue. Researches show that the plant stem cells have important application values for improving the metabolism of the skin, improving the microcirculation, tightening and recovering the elasticity of the skin. The hypsizygus marmoreus callus culture filtrate is derived from plant stem cells extracted from roots of hypsizygus marmoreus callus culture, and the stem cells contain phenolic acid, chlorogenic acid, flavonoid and the like, so that the hypsizygus marmoreus callus culture can resist extreme environments of Gastrick and high ultraviolet, can accelerate metabolism of epidermis, effectively improve self-healing capability of skin, quickly repair damaged parts and promote regeneration of cell tissues.

In some embodiments, the composition comprises the following parts by weight of each:

in some embodiments, the composition comprises the following parts by weight of each:

in some embodiments, the hypecoum vulgare callus culture filtrate is obtained by:

1) slicing the pimpinella anisum root, putting the sliced pimpinella anisum root into a basic culture medium, and adding sucrose, agar and dichlorophenoxyacetic acid for culturing to obtain callus;

2) placing the callus prepared in the step 1) in a basic culture medium, and adding sucrose, dichlorophenoxyacetic acid and kinetin for culturing to obtain the suspension cells.

In some embodiments, in the step 1), the sucrose is added into the minimal medium by mass fraction of 1.5-4.5%, the agar is added by mass fraction of 7-8.5 g/L, and the dichlorophenoxyacetic acid is added by mass fraction of 0.5-1.5 mg/L; preferably, step 1) culturing for 20-30 days; further preferably, the culture is carried out for 25 days.

In some embodiments, in the step 2), the sucrose is added into the minimal medium by mass fraction of 1.5-4.5%, the addition amount of the dichlorophenoxyacetic acid is 0.2-1.2 mg/L, and the addition amount of the kinetin is 0.01-0.5 mg/L; preferably, the culturing in step 2) is carried out for 20 to 30 days; further preferably, the culture is carried out for 25 days.

In some embodiments, the preparing of the hypecoum vulgare callus culture filtrate further comprises the steps of filtering the prepared suspension cells and collecting the filtrate.

The application also provides the application of the composition in preparing a skin care product with the function of repairing blue light damage; preferably, the skin care product comprises one or more of eye cream, face cream, essence and toner.

In one aspect, the present application also provides a cosmetic comprising the composition.

In some embodiments, the composition is added in an amount of 3 to 20%, preferably 6 to 16%.

In one aspect, the present application further provides the cream, which comprises the following components in percentage by weight:

preferably, the addition amount of the composition is 6-16%; more preferably 10%.

In one aspect, the present application also provides a method for preparing the cream containing the repair blue light damage composition, which is characterized by comprising the following steps:

(1) putting the phase A into a water phase pot, uniformly stirring, heating and stirring to 80-85 ℃, and preserving heat for 8-20 min;

(2) putting the phase B into an oil phase pot, heating and stirring to 80-85 ℃, and stirring;

(3) and pumping the materials in the water phase pot into an emulsifying pot. Pumping the materials in the oil phase pot into an emulsifying pot, stirring, vacuumizing, homogenizing, and stirring for 10-25 min;

(4) cooling to 40-45 deg.C, adding phase C, and stirring.

Drawings

FIG. 1 is a graph of cell activity of each group in a repair blue light damage assay;

FIG. 2 is a graph showing DPPH scavenging efficiency of the creams of examples 1-4 and comparative examples 1-16 in DPPH radical scavenging experimental tests;

FIG. 3 is a graph showing the change in skin elasticity of the creams of examples 1 to 4 and comparative examples 1 to 16 in the skin elasticity test.

Detailed Description

The technical solutions of the present application are further illustrated by the following specific examples, which do not represent a limitation to the scope of the present application. Insubstantial modifications and adaptations of the concepts taught herein by others are intended to be covered by the present disclosure.

"comprising" or "including" is intended to mean that the compositions (e.g., media) and methods include the recited elements, but not excluding others. When used in defining compositions and methods, "consisting essentially of … …" is meant to exclude other elements having any significance to the combination of the stated objects. Thus, a composition consisting essentially of the elements defined herein does not exclude other materials or steps that do not materially affect the basic and novel characteristics of the claimed application. "consisting of … …" refers to trace elements and substantial process steps excluding other components. Embodiments defined by each of these transition terms are within the scope of the present application.