Centrifugal machine for stem cell separation

文档序号:1091510 发布日期:2020-09-25 浏览:43次 中文

阅读说明:本技术 一种干细胞分离用离心机 (Centrifugal machine for stem cell separation ) 是由 陈龙刚 于 2020-06-28 设计创作,主要内容包括:本发明公开了一种干细胞分离用离心机,包括壳体和转轴,所述壳体内部设置有离心驱动装置与平衡度检测装置,且平衡度检测装置设置在离心驱动装置外侧,所述壳体顶部设置有箱盖,且箱盖下侧通过密封机构与壳体相连接,所述箱盖内部设置有二次稳定装置,且二次稳定装置与离心驱动装置上端相连接,所述壳体左方设置有抽真空设备,且壳体下方设置有加固机构,所述离心驱动装置包括主轴。该干细胞分离用离心机,设置有梯形槽和梯形台,箱盖固定在壳体顶部时,梯形台贴合设置在梯形槽内部,梯形槽外侧的转动件通过第二滚珠轴承对梯形台以及梯形台下侧的主轴起到支撑作用,使主轴转动更加稳定,提高了离心机的使用寿命。(The invention discloses a centrifugal machine for stem cell separation, which comprises a shell and a rotating shaft, wherein a centrifugal driving device and a balance degree detection device are arranged in the shell, the balance degree detection device is arranged outside the centrifugal driving device, a box cover is arranged at the top of the shell, the lower side of the box cover is connected with the shell through a sealing mechanism, a secondary stabilizing device is arranged in the box cover and connected with the upper end of the centrifugal driving device, a vacuumizing device is arranged on the left side of the shell, a reinforcing mechanism is arranged below the shell, and the centrifugal driving device comprises a main shaft. This centrifuge is used in stem cell separation is provided with dovetail groove and trapezoidal platform, and when the case lid was fixed at the casing top, the laminating of trapezoidal platform set up at the dovetail groove inside, and the rotation piece in the trapezoidal groove outside plays the supporting role to the main shaft of trapezoidal platform and trapezoidal platform downside through the second ball bearing, makes the main shaft rotate more stably, has improved centrifuge's life.)

1. A centrifuge for stem cell separation, comprising a housing (1) and a rotating shaft (54), characterized in that: casing (1) inside centrifugal drive device (2) and the equilibrium detection device (3) of being provided with, and equilibrium detection device (3) set up in the centrifugal drive device (2) outside, casing (1) top is provided with case lid (4), and case lid (4) downside is connected with casing (1) through sealing mechanism (5), case lid (4) inside is provided with secondary stabilization device (6), and secondary stabilization device (6) are connected with centrifugal drive device (2) upper end, casing (1) left is provided with evacuation equipment (7), and casing (1) below is provided with strengthening mechanism (8), centrifugal drive device (2) include:

the lower side of the main shaft (20) is rotatably arranged in the shell (1) through a first ball bearing (21), the lower end of the main shaft (20) is connected with a high-speed motor (22), the high-speed motor (22) is arranged on the lower side of the shell (1), a centrifugal cavity (23) is formed in the upper side of the shell (1), the main shaft (20) is arranged in the centrifugal cavity (23), a rotating head (24) is connected to the outer side of the middle of the main shaft (20) through a spline, and a lifting bucket (25) is rotatably connected to the rotating head (24);

the balance detection device (3) includes:

the centrifugal pump comprises a lifting table (30) which is arranged on the lower side in a centrifugal cavity (23) in a sliding mode, a pressure sensor (31) is arranged at the top of the lifting table (30), the inner side of the lifting table (30) is in threaded connection with the outer side of a driving ring (32), the upper end of the inner side of the driving ring (32) is in rotary connection with a shell (1), the lower end of the inner side of the driving ring (32) is in meshed connection with a stepping motor (33), the stepping motor (33) is arranged in the shell (1), supporting legs (81) are arranged at the bottom of the shell (1), a signal processor (34) and an inclination angle sensor (35) are arranged in the supporting legs (81), and the inclination angle sensor (35) is arranged on the lower side;

the sealing mechanism (5) includes:

locating holes (50) which are four in number, the locating holes (50) are arranged at the top of the shell (1), the lower ends of the locating holes (50) are communicated with the blind holes (51), the sliding parts (52) are connected inside the blind holes (51) in a sliding mode, compression springs (53) are arranged between the bottoms of the sliding parts (52) and the blind holes (51), first locating columns (55) are welded at the two ends of the rotating shaft (54), the lower ends of the first locating columns (55) are welded at the top of the sliding parts (52) on the rear side of the shell (1), the rotating shaft (54) is rotatably connected with the rear end of the box cover (4), second locating columns (56) are welded at the front side of the box cover (4), the lower ends of the second locating columns (56) are attached to the top of the sliding parts (52) on the front side of the shell (1), metal blocks (57) are arranged on the left side and the right side of the bottom of the shell, the electromagnets (58) are arranged on the left side and the right side of the top of the shell (1), and a sealing ring (59) is arranged between the lower end of the box cover (4) and the top of the shell (1);

the secondary stabilization device (6) comprises:

the trapezoidal groove (60) is a trapezoidal platform-shaped groove with a narrow upper part and a wide lower part, the trapezoidal groove (60) is arranged on the outer side of the trapezoidal platform (61), the trapezoidal platform (61) is in threaded connection with the outer side of the upper end of the device, the trapezoidal groove (60) is formed in the bottom of the rotating piece (62), and the rotating piece (62) is in rotating connection with the lower end of the box cover (4) through a second ball bearing (63);

the vacuum-pumping device (7) comprises:

the right end of the exhaust pipe (70) penetrates through the shell (1) and is communicated with the left end of the centrifugal cavity (23), the left end of the exhaust pipe (70) is communicated with a vacuum pump (72) through an electromagnetic three-way valve (71), the electromagnetic three-way valve (71) is arranged at the top of the vacuum pump (72), and the vacuum pump (72) is arranged on the left side of the shell (1);

the reinforcement mechanism (8) includes:

and the vacuum sucker (80) is arranged on the outer side of the support leg (81), the vacuum sucker (80) is communicated with the vacuum pump (72) through the electromagnetic three-way valve (71), and the vacuum sucker (80) and the support leg (81) are both arranged at the bottom of the shell (1).

2. The centrifuge for stem cell separation according to claim 1, wherein: the number of the lifting buckets (25) is four, and the joints of the lifting buckets (25) and the rotating heads (24) are positioned above the gravity centers of the lifting buckets (25).

3. The centrifuge for stem cell separation according to claim 1, wherein: the sliding distance of the lifting platform (30) is larger than the distance between the uppermost end of the lifting platform (30) and the lowermost end of the bucket (25).

4. The centrifuge for stem cell separation according to claim 1, wherein: the pressure sensor (31) is arranged right below the bucket (25).

5. The centrifuge for stem cell separation according to claim 1, wherein: the signal processor (34) is electrically connected with the pressure sensor (31), the high-speed motor (22), the stepping motor (33), the electromagnet (58), the vacuum pump (72) and the inclination angle sensor (35).

6. The centrifuge for stem cell separation according to claim 1, wherein: the number of the positioning holes (50) is four, the four positioning holes (50) are respectively arranged at four corners of the top of the shell (1), and meanwhile, the inner walls of the positioning holes (50) are attached to the outer ends of the first positioning columns (55) and the outer ends of the second positioning columns (56).

7. The centrifuge for stem cell separation according to claim 1, wherein: the compression distance of the compression spring (53) is greater than the height of the second positioning column (56) and the height of the metal block (57).

8. The centrifuge for stem cell separation according to claim 1, wherein: the inner wall of the trapezoid groove (60) is attached to the outer end of the trapezoid table (61), and the center line of the trapezoid table (61), the center line of the main shaft (20) and the center line of the rotating piece (62) are located on the same vertical line.

9. The centrifuge for stem cell separation according to claim 1, wherein: the vacuum suckers (80) are respectively arranged at four corners of the bottom of the shell (1), and the lowest ends of the vacuum suckers (80) and the lowest ends of the support legs (81) are located on the same horizontal plane.

Technical Field

The invention relates to the technical field of centrifuges, in particular to a centrifuge for separating stem cells.

Background

The centrifuge is a professional separation equipment for separating and purifying stem cells by utilizing centrifugal force generated by rapid rotation, when the centrifuge runs, the rotating speed of an inner rotating head of the centrifuge normally exceeds 10000/min, but the existing centrifuge is not provided with a fastening mechanism and is only fixed on a workbench under the pressure of dead weight, so that the centrifuge is easy to generate self vibration along with the rotation of the rotating head, the rotating stability of the rotating head in the centrifuge is deteriorated, the service life of the centrifuge is influenced, the existing centrifuge only rotationally fixes the lower side of a main shaft, the main shaft drives the rotating head to rotate to generate axis deviation, the running stability of the centrifuge is further reduced, the existing centrifuge is not provided with a vacuum pumping device, the wind resistance coefficients of the inner rotating head and a hanging bucket are high, when the rotating head rotates at high speed, the air resistance greatly influences the rotating effect of the rotating head, the centrifugal machine needs to be guaranteed to be stressed symmetrically and balanced on the rotary head before running, the rotary head is kept in a horizontal state, the existing centrifugal machine is not provided with a balance safety structure, the quality of a test tube is only measured manually, and in case of misoperation, accidents occur during the running of the centrifugal machine easily, so that the centrifugal machine is damaged, and the centrifugal machine needs to be improved aiming at the problems.

Disclosure of Invention

The invention aims to provide a centrifuge for stem cell separation, which aims to solve the problems that the existing centrifuge proposed in the background technology is not provided with a fastening mechanism and is fixed on a workbench only under the pressure of self weight, so that the centrifuge is easy to generate self vibration along with the rotation of a rotor, the rotation stability of the rotor in the centrifuge is deteriorated, the service life of the centrifuge is influenced, the existing centrifuge only carries out rotation fixing on the lower side of a main shaft, the main shaft generates axis deviation when driving the rotor to rotate, the stability of the centrifuge in operation is further reduced, the existing centrifuge is not provided with a vacuum pumping device generally, the wind resistance coefficient of the rotor and a bucket in the existing centrifuge is high, the rotation effect of the rotor is greatly influenced by the air resistance when the rotor rotates at high speed, the centrifuge needs to ensure that the stress on the rotor is kept symmetrical and balanced, and the rotor is kept in a horizontal state before operation, and current centrifuge is not provided with balanced insurance structure, only relies on artifical measurement test tube quality, in case take place misoperation, makes the centrifuge operation accident very easily and leads to the problem of centrifuge damage.

In order to achieve the purpose, the invention provides the following technical scheme: the utility model provides a centrifuge for stem cell separation, includes casing and pivot, inside centrifugal drive device and the equilibrium detection device of being provided with of casing, and the equilibrium detection device setting is in the centrifugal drive device outside, the casing top is provided with the case lid, and the case lid downside is connected with the casing through sealing mechanism, the inside secondary stabilising arrangement that is provided with of case lid, and secondary stabilising arrangement are connected with centrifugal drive device upper end, the casing left is provided with evacuation equipment, and is provided with reinforcing mechanism below the casing, centrifugal drive device includes:

the lower side of the main shaft is rotatably arranged in the shell through a first ball bearing, the lower end of the main shaft is connected with a high-speed motor, the high-speed motor is arranged on the lower side of the shell, a centrifugal cavity is formed in the upper side of the shell, the main shaft is arranged in the centrifugal cavity, a rotating head is connected to the outer side of the middle of the main shaft through a spline, and a bucket is rotatably connected to the rotating head;

the balance degree detection device includes:

the centrifugal pump comprises a centrifugal cavity, a lifting table, a driving ring, a stepping motor, a signal processor, an inclination angle sensor and a power supply, wherein the lifting table is arranged on the lower side in the centrifugal cavity in a sliding mode, the top of the lifting table is provided with the pressure sensor, the inner side of the lifting table is in threaded connection with the outer side of the driving ring, the upper end of the inner side of the driving ring is rotatably connected with a shell, the lower end of the inner side of the driving ring is in meshed connection with the stepping motor, the stepping motor is arranged in the shell, the bottom of the;

the sealing mechanism includes:

the four positioning holes are formed in the top of the shell, the lower ends of the positioning holes are communicated with the blind holes, sliding parts are connected in the blind holes in a sliding mode, compression springs are arranged between the bottoms of the sliding parts and the blind holes, first positioning columns are welded at two ends of the rotating shaft, the lower ends of the first positioning columns are welded at the top of the sliding parts on the rear side of the shell, the rotating shaft is rotatably connected with the rear end of the box cover, second positioning columns are welded at the front side of the box cover, the lower ends of the second positioning columns are attached to the top of the sliding parts on the front side of the shell, metal blocks are arranged on the left side and the right side of the bottom of the shell, penetrate through the shell and are connected with the electromagnets, the electromagnets are;

the secondary stabilization device includes:

the trapezoidal groove is a trapezoidal platform-shaped groove with a narrow upper part and a wide lower part, the trapezoidal groove is arranged on the outer side of the trapezoidal platform, the trapezoidal platform is in threaded connection with the outer side of the upper end of the device, the trapezoidal groove is formed in the bottom of the rotating part, and the rotating part is in rotating connection with the lower end of the box cover through a second ball bearing;

the vacuum pumping apparatus includes:

the right end of the exhaust pipe penetrates through the shell and is communicated with the left end of the centrifugal cavity, the left end of the exhaust pipe is communicated with the vacuum pump through the electromagnetic three-way valve, the electromagnetic three-way valve is arranged at the top of the vacuum pump, and the vacuum pump is arranged on the left side of the shell;

the reinforcement mechanism includes:

and the vacuum chuck is arranged outside the support leg, is communicated with the vacuum pump through an electromagnetic three-way valve, and is arranged at the bottom of the shell together with the support leg.

Preferably, the number of the lifting buckets is four, and the connecting positions of the lifting buckets and the rotating heads are located above the gravity centers of the lifting buckets.

Preferably, the sliding distance of the lifting platform is greater than the distance between the uppermost end of the lifting platform and the lowermost end of the bucket.

Preferably, the pressure sensor is arranged right below the bucket.

Preferably, the signal processor is electrically connected with the pressure sensor, the high-speed motor, the stepping motor, the electromagnet, the vacuum pump and the inclination angle sensor.

Preferably, the number of the positioning holes is four, the four positioning holes are respectively arranged at four corners of the top of the shell, and the inner wall of each positioning hole is attached to the outer end of the first positioning column and the outer end of the second positioning column.

Preferably, the compression distance of the compression spring is greater than the height of the second positioning column and the height of the metal block.

Preferably, the inner wall of the trapezoidal groove is attached to the outer end of the trapezoidal table, and the central line of the trapezoidal table, the central line of the main shaft and the central line of the rotating part are located on the same vertical straight line.

Preferably, the vacuum chucks are respectively arranged at four corners of the bottom of the shell, and the lowest ends of the vacuum chucks and the lowest ends of the support legs are positioned on the same horizontal plane.

Compared with the prior art, the invention has the beneficial effects that: the stem cell is separated by a centrifugal machine,

(1) the centrifugal machine is provided with a lifting platform, an inclination angle sensor and a pressure sensor, before the centrifugal machine is ready to operate, the inclination angle sensor automatically detects the levelness of the shell, a stepping motor pushes the lifting platform to ascend through a driving ring until the pressure sensor contacts the lower ends of four lifting buckets, the pressure sensor detects the quality of the lifting buckets, and a signal sensor judges whether the centrifugal machine can operate or not according to detection information, so that the problem that the centrifugal machine is damaged easily due to misoperation of personnel is avoided;

(2) the vacuum pump pumps air in a centrifugal cavity through the exhaust pipe to form vacuum in the centrifugal cavity, so that the air in the centrifugal cavity is prevented from blocking the rotation of the rotary head;

(3) the centrifugal machine is provided with a trapezoidal groove and a trapezoidal table, when the box cover is fixed on the top of the shell, the trapezoidal table is attached to the inside of the trapezoidal groove, and a rotating piece on the outer side of the trapezoidal groove supports the trapezoidal table and a main shaft on the lower side of the trapezoidal table through a second ball bearing, so that the main shaft rotates more stably, and the service life of the centrifugal machine is prolonged;

(4) be provided with vacuum chuck and stabilizer blade, the casing passes through the stabilizer blade and places on the workstation, and the vacuum pump passes through vacuum chuck extraction air, makes vacuum chuck and workstation be connected the fastening, plays and consolidates centrifuge, has avoided centrifuge to produce the problem of natural vibration along with the main shaft rotation easily.

Drawings

FIG. 1 is a schematic cross-sectional front view of the present invention;

FIG. 2 is a schematic top view of the cross-sectional structure of the present invention;

FIG. 3 is a schematic diagram of a left side view cross-sectional structure of the present invention;

FIG. 4 is a schematic left side sectional view of the housing of the present invention;

FIG. 5 is a bottom view of the housing of the present invention;

FIG. 6 is a schematic top sectional view of the housing of the present invention;

FIG. 7 is an enlarged view of the structure at A in FIG. 1 according to the present invention;

fig. 8 is a schematic diagram of the work flow structure of the present invention.

In the figure: 1. a housing, 2, a centrifugal driving device, 20, a main shaft, 21, a first ball bearing, 22, a high-speed motor, 23, a centrifugal cavity, 24, a rotary head, 25, a bucket, 3, a balance degree detection device, 30, a lifting platform, 31, a pressure sensor, 312, a driving ring, 33, a stepping motor, 34, a signal processor, 35, an inclination angle sensor, 4, a box cover, 5, a sealing mechanism, 50, a positioning hole, 51, a blind hole, 52, a sliding part, 53, a compression spring, 54, a rotating shaft, 55, a first positioning column, 56, a second positioning column, 57, a metal block, 58, an electromagnet, 59, a sealing ring, 6, a secondary stabilizing device, 60, a trapezoidal groove, 61, a trapezoidal platform, 62, a rotating part, 63, a second ball bearing, 7, a vacuum pumping device, 70, an exhaust pipe, 71, an electromagnetic three-way valve, 72, a vacuum pump, 8, a reinforcing mechanism, 80, a vacuum suction cup, 81. and (3) a support leg.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Referring to fig. 1-8, the present invention provides a technical solution: the utility model provides a stem cell is centrifuge for separation, as shown in fig. 1, fig. 2 and fig. 3, casing 1 is inside to be provided with centrifugal drive device 2 and equilibrium detection device 3, and equilibrium detection device 3 sets up in the centrifugal drive device 2 outside, casing 1 top is provided with case lid 4, and case lid 4 downside is connected with casing 1 through sealing mechanism 5, the inside secondary stabilising arrangement 6 that is provided with of case lid 4, and secondary stabilising arrangement 6 is connected with centrifugal drive device 2 upper end, casing 1 left is provided with evacuation equipment 7, and casing 1 below is provided with reinforcing mechanism 8, centrifugal drive device 2 includes: the main shaft 20, its downside rotates through first ball bearing 21 and sets up inside casing 1, the main shaft 20 lower extreme is connected with high-speed motor 22, and high-speed motor 22 sets up at casing 1 downside, centrifugal chamber 23 has been seted up to casing 1 upside, and be provided with main shaft 20 in the centrifugal chamber 23, main shaft 20 middle part outside splined connection has rotor 24, and rotate on rotor 24 and be connected with well-bucket 25, well-bucket 25 is provided with four, and well-bucket 25 is located well-bucket 25 focus top with rotor 24 junction, be convenient for well-bucket 25 stable hang on rotor 24.

As shown in fig. 1, 6, and 8, the balance detecting device 3 includes: the elevating platform 30 is arranged at the lower side in the centrifugal cavity 23 in a sliding manner, the sliding distance of the elevating platform 30 is greater than the distance between the uppermost end of the elevating platform 30 and the lowermost end of the bucket 25, the pressure sensor 31 is arranged under the bucket 25, so that the stepping motor 33 can drive the driving ring 32 to rotate, the driving ring 32 can drive the elevating platform 30 to ascend until the pressure sensor 31 at the top of the elevating platform 30 contacts the lowermost end of the bucket 25, the pressure sensor 31 is arranged at the top of the elevating platform 30, the inner side of the elevating platform 30 is in threaded connection with the outer side of the driving ring 32, the upper end of the inner side of the driving ring 32 is in rotational connection with the casing 1, the lower end of the inner side of the driving ring 32 is in meshed connection with the stepping motor 33, the stepping motor 33 is arranged in the casing 1, the bottom of the casing 1 is provided with a support leg 81, the support leg 81, the signal processor 34 is electrically connected with the pressure sensor 31, the high-speed motor 22, the stepping motor 33, the electromagnet 58, the vacuum pump 72 and the inclination angle sensor 35, so that the signal processor 34 can collect the position states of the components in the centrifuge according to the inclination angle sensor 35 and the pressure sensor 31, and judge whether to start the centrifuge according to the position states.

As shown in fig. 1, 4, and 7, the sealing mechanism 5 includes: four positioning holes 50 are arranged, four positioning holes 50 are respectively arranged at four corners of the top of the shell 1, the inner wall of each positioning hole 50 is attached to the outer end of a first positioning column 55 and the outer end of a second positioning column 56, when the second positioning column 56 enters the positioning hole 50 at the front side of the shell 1, the central line of the trapezoidal platform 61 and the central line of the trapezoidal groove 60 are positioned on the same vertical line, the positioning hole 50 is arranged at the top of the shell 1, the lower end of each positioning hole 50 is communicated with the blind hole 51, a sliding part 52 is connected in the blind hole 51 in a sliding manner, a compression spring 53 is arranged between the bottom of the sliding part 52 and the blind hole 51, the compression distance of the compression spring 53 is greater than the height of the second positioning column 56 and the height of the metal block 57, the compression spring 53 is convenient to push the second positioning column 56 to, the lower end of the first positioning column 55 is welded on the top of the rear sliding part 52 of the shell 1, meanwhile, the rotating shaft 54 is rotatably connected with the rear end of the box cover 4, the second positioning column 56 is welded on the front side of the box cover 4, the lower end of the second positioning column 56 is attached to the top of the front sliding part 52 of the shell 1, metal blocks 57 are arranged on the left side and the right side of the bottom of the shell 1, the metal blocks 57 penetrate through the shell 1 and are connected with the electromagnets 58, the electromagnets 58 are arranged on the left side and the right side of the top of the shell 1, and a sealing ring 59 is.

As shown in fig. 1 and 3, the secondary stabilizing device 6 includes: trapezoidal groove 60, it is the trapezoidal platform form recess of width under the narrow upper portion, trapezoidal groove 60 inner wall laminating trapezoidal platform 61 outer end, and the central line of trapezoidal platform 61 and the central line of main shaft 20 and the central line of rotating piece 62 are located same vertical straight line, make the rotation piece 62 cooperation second ball bearing 63 in the trapezoidal groove 60 outside start the effect of supplementary fixed to main shaft 20, trapezoidal groove 60 sets up in the trapezoidal platform 61 outside, and trapezoidal platform 61 threaded connection is in the device upper end outside, trapezoidal groove 60 sets up in rotating piece 62 bottom, and rotate piece 62 and be connected through second ball bearing 63 and case lid 4 lower extreme rotation.

As shown in fig. 1, 4 and 5, the vacuum evacuation apparatus 7 includes: the right end of the exhaust pipe 70 penetrates through the shell 1 to be communicated with the left end of the centrifugal cavity 23, the left end of the exhaust pipe 70 is communicated with a vacuum pump 72 through an electromagnetic three-way valve 71, the electromagnetic three-way valve 71 is arranged at the top of the vacuum pump 72, and the vacuum pump 72 is arranged on the left side of the shell 1; the reinforcement mechanism 8 includes: vacuum chuck 80, it sets up in the stabilizer blade 81 outside, and vacuum chuck 80 sets up respectively in casing 1 bottom four corners, and the lowermost extreme of vacuum chuck 80 and stabilizer blade 81 are located same horizontal plane, makes stabilizer blade 81 support casing 1, and vacuum chuck 80 plays the effect of fixed casing 1 simultaneously, and vacuum chuck 80 is linked together through electromagnetism three-way valve 71 and vacuum pump 72, and vacuum chuck 80 all sets up in casing 1 bottom with stabilizer blade 81.

The working principle is as follows: when the stem cell separation centrifuge is used, the model of the pressure sensor 31 is MS5837-02BA, the model of the signal processor 34 is TMS320F28016PZA, the model of the electromagnetic three-way valve 71 is VT307-5G-01, the model of the inclination angle sensor 35 is MRT303G01, the shell 1 is placed on the upper surface of a workbench through a support 81, an external power supply is connected, the vacuum pump 72 and the electromagnetic three-way valve 71 are started, the vacuum pump 72 pumps air to the vacuum suction cup 80 through the electromagnetic three-way valve 71, the shell 1 is fixed and fastened with the workbench through the vacuum suction cup 80, the problem that the running state of the centrifuge is influenced by self-vibration generated during the running of the centrifuge is avoided, before the centrifuge runs, a test tube is put into the hanging bucket 25, the stepping motor 33 is started, the stepping motor 33 drives the driving ring 32 to rotate, the driving ring 32 pushes the lifting platform 30 to rise until the pressure sensor 31 at the, the pressure sensor 31 detects the driving quality of each bucket 25, the inclination angle sensor 35 detects the horizontal angle of the shell 1, the signal processor 34 judges whether the centrifuge can be operated according to the detection information, the problem that the centrifuge is easy to operate to cause errors and cause damage to the centrifuge is solved, the cover 4 is rotated by taking the rotating shaft 54 as the center, then the cover 4 is pressed, the first positioning column 55 and the second positioning column 56 respectively slide into the positioning holes 50, the metal block 57 is attached to the electromagnet 58, the electromagnet 58 is started, the electromagnet 58 is matched with the metal block 57 to fix the cover 4, the cover 4 is in sealing connection with the shell 1 through the sealing ring 59, the centrifugal cavity 23 becomes a sealing space, the vacuum pump 72 and the electromagnetic three-way valve 71 are started, the vacuum pump 72 pumps air in the centrifugal cavity 23 through the exhaust pipe 70, the centrifugal cavity 23 is vacuumized, and the air resistance borne by the rotating head 24, the using effect of the centrifuge is improved, when the box cover 4 rotates, the trapezoidal groove 60 is arranged right above the trapezoidal table 61, after the box cover 4 is closed by pressing, the rotating part 62 is tightly attached to the trapezoidal table 61 through the trapezoidal groove 60, the trapezoidal table 61 is in threaded connection with the outer side of the upper end of the main shaft 20, the rotating part 62 is matched with the second ball bearing 63 to play a role in supporting the main shaft 20, so that the main shaft 20 rotates more stably, the high-speed motor 22 is started, the high-speed motor 22 drives the rotating head 24 and the bucket 25 to rotate through the main shaft 20 to separate and purify stem cells in a test tube, the main shaft 20 rotates more smoothly through the first ball bearing 21, after the centrifuge finishes running, the electromagnet 58 is turned off, the compression spring 53 pushes the second positioning column 56 and the first positioning column 55 to move upwards through the sliding part 52 until the second positioning column 56 is separated from the positioning hole 50, the trapezoidal table 61 is separated from the, this completes the work and is not described in detail in this specification as it is well known in the art.

The terms "central," "longitudinal," "lateral," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," and the like are used in the orientation or positional relationship indicated in the drawings for simplicity of description only and are not intended to indicate or imply that the referenced devices or elements must be in a particular orientation, constructed and operative in a particular orientation, and are not to be considered limiting of the claimed invention.

Although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that various changes in the embodiments and/or modifications of the invention can be made, and equivalents and modifications of some features of the invention can be made without departing from the spirit and scope of the invention.

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