阅读说明：本技术 一种低聚异麦芽糖的制备方法 (Preparation method of isomaltooligosaccharide ) 是由 宋建民 王德海 宛荣生 张琴 王颂 黄祥君 于 2020-06-15 设计创作，主要内容包括：本发明公开了一种低聚异麦芽糖的制备方法,具体包括以下步骤：S1、淀粉的制备：选取适量品种优良的小麦,将小麦中的杂物进行清除,小麦粒经清理后,进行着水调制,调制后小麦水分控制在15～19％,然后输送到剥皮设备内进行皮层的分离,剥皮后的小麦再次用水进行调制,使小麦中的总水分达到20％-25％,然后对小麦进行破胚处理,破胚后进行筛分,本发明涉及低聚异麦芽糖制备技术领域。该低聚异麦芽糖的制备方法,通过三糖”纯度高,达到95％以上,糖浆粘度小,甜度高,同时提高二次喷射液化温度,保证了料液的充分液化,提高了蛋白絮凝效果,有利于下一步的糖化反应,需水料比较小就能达到较好的分离效果。(The invention discloses a preparation method of isomaltooligosaccharide, which comprises the following steps: s1, preparation of starch: selecting a proper amount of good-variety wheat, removing impurities in the wheat, cleaning wheat grains, then wetting and modulating the wheat grains, controlling the moisture of the modulated wheat to be 15-19%, then conveying the wheat grains to a peeling device for separation of a skin layer, modulating the peeled wheat grains with water again to enable the total moisture in the wheat to reach 20% -25%, then performing embryo breaking treatment on the wheat grains, and screening the wheat grains after embryo breaking. According to the preparation method of isomaltooligosaccharide, the purity of trisaccharide is high and reaches more than 95%, the syrup is low in viscosity and high in sweetness, the secondary injection liquefaction temperature is increased, the full liquefaction of the feed liquid is guaranteed, the protein flocculation effect is improved, the next saccharification reaction is facilitated, and the good separation effect can be achieved by the aid of a small water-to-material ratio.)

1. A method for preparing isomaltooligosaccharide is characterized in that: the method specifically comprises the following steps:

s1, preparation of starch: selecting a proper amount of excellent wheat, removing impurities in the wheat, cleaning wheat grains, performing water-dressing modulation, controlling the moisture of the modulated wheat to be 15-19%, conveying the wheat grains to peeling equipment for separating the skin layer, modulating the peeled wheat with water again to enable the total moisture in the wheat to reach 20% -25%, then performing embryo breaking treatment on the wheat, screening after embryo breaking to enable the endosperm and the embryo to be completely separated, and pouring the separated endosperm into a flour mill to be crushed into 80-100-mesh wheat flour;

s2, preparation of a liquefied liquid: adding deionized water into a wheat flour raw material to mix into starch milk, wherein the ratio of the deionized water to the wheat flour is 1:3-1:5, the starch milk is adjusted to have a starch milk concentration of 16-19 degrees Be and a pH value of 5.5-6.0, maltotriose generating enzyme and pullulanase are added, the addition amount of the maltotriose generating enzyme is 6.0 multiplied by 105-9.6 multiplied by 105U per ton of the starch raw material, the addition amount of the pullulanase is 3.0 multiplied by 105-6.0 multiplied by 105ASPU per ton of the starch raw material, after the starch is subjected to heat preservation and saccharification for 36-56 hours, enzyme activity is inactivated at 80-90 ℃ to prepare saccharified liquid, alpha-high temperature amylase is added into the starch slurry with the temperature reduced to 30-40 ℃ according to the ratio of 0.23-0.35L of the alpha-high temperature amylase per ton of the starch, and spray liquefaction is carried out at the temperature of 105-109 ℃ to prepare liquefied liquid with the temperature of 93-97 ℃;

s3, saccharification and glucoside conversion: cooling the liquefied solution to 55-65 ℃, adding fungal enzyme which is 0.04-0.06% of the weight of the wheat flour, preserving the heat for 30-40 h, adding transglycosidase which is 0.3-0.5% of the weight of the wheat flour, and maintaining the temperature for 15-25 h at 50-60 ℃;

s4, decoloring treatment: adjusting the pH value of the feed liquid after enzyme deactivation to 4.5-5.5, adding a flocculating agent, standing at 75-85 ℃, keeping the temperature for 30-40 min, adding 1-3% of active carbon, stirring for 30-40 min, and filtering the feed liquid;

s5, subsequent processing: and (2) carrying out ion exchange on the feed liquid in the S4 by using an ion exchange system, wherein the feeding temperature is 40-50 ℃, the flow rate is 12-15 m3/h, the running time is 90-120 min, strong acid and weak base resin is filled in an ion exchange column to prepare refined sugar liquid, the feed liquid after ion exchange is concentrated to 60-65% in volume and then enters a chromatographic separation system, the chromatographic running pressure is 0.25-0.35 MPa, the temperature is 65-70 ℃, the water consumption ratio is 1: 1.2-1: 1.5, 1.4-1.7 m3 is fed per hour, sugar with the polymerization degree of more than or equal to 3 is collected, and then the isomaltooligosaccharide is prepared by concentrating and drying.

2. The method according to claim 1, wherein the isomaltooligosaccharide comprises: in the step 3, the transglucosidase is alpha-glucosyltransferase, and the dosage of the transglucosidase is 0.6-0.8 kg/ton based on the dry weight of the starch.

3. The method according to claim 1, wherein the isomaltooligosaccharide comprises: and the injection liquefaction in the step 2 is two-time injection liquefaction, the temperature of the first injection liquefaction is 100-110, and the temperature of the second injection liquefaction is 130-140.

4. The method according to claim 1, wherein the isomaltooligosaccharide comprises: and 4, filtering in the step 4 by adopting a plate-and-frame filter, wherein the filtering pressure is 0.35Mpa, and the water flow is 5.5-6.5 t/h.

5. The method according to claim 1, wherein the isomaltooligosaccharide comprises: and the concentration in the step 5 is heating evaporation concentration until the mass concentration of the isomaltooligosaccharide is 50-70% and the pH is 4.2-5.8.

6. The method according to claim 1, wherein the isomaltooligosaccharide comprises: the drying in the step 5 is drying by a spray drying tower or a vacuum drying device.

Technical Field

The invention relates to the technical field of isomaltooligosaccharide preparation, and in particular relates to a method for preparing isomaltooligosaccharide.

Background

Isomalto-oligosaccharides (IMO), also known as isomaltooligosaccharides, branched oligosaccharides, etc., exist in a very small amount in a free state in nature, but as a component of amylopectin or polysaccharides, a small amount exists in some fermented foods such as soy sauce, yellow wine or enzymatic glucose syrup, and the industrial production of isomaltooligosaccharides from starch requires an enzyme which is an alpha-glucosidase, also known as glucosyltransferase, abbreviated as alpha-glucosidase, and which cleaves the alpha-1, 6 glycosidic bond in the molecular structure of maltose and malto-oligosaccharide and transfers a free glucose residue to the alpha-1, 6 position in another glucose molecule or molecule of maltose or maltotriose to form isomaltooligosaccharides, isomaltotetraose, isomaltotriose, panose, etc., and the Isomaltooligosaccharides (IMO), also known as isomaltooligosaccharides, branched oligosaccharides, etc, Isomalto-oligosaccharide, branched oligosaccharide and the like are determined as isomalto-oligosaccharide in the light industry standard of China, the isomalto-oligosaccharide is one of starch sugar, the main components are isomalto-oligosaccharide, panose, isomaltotriose and oligosaccharide above tetrasaccharide which are combined by alpha-1, 6 glycosidic bonds among glucose molecules, and foreign and domestic scholars have a little difference in the description of the types of sugar contained in the isomalto-oligosaccharide (IMO), but the foreign and domestic scholars generally agree to include isomalto, panose and isomaltotriose, the isomalto-oligosaccharide is a main component for embodying the functionality of the isomalto-oligosaccharide (IMO), the content of the isomalto-oligosaccharide reflects the quality of the product, the price and the application prospect of the product are also influenced, the isomalto-oligosaccharide is used as a component of amylopectin or polysaccharide in nature, only a small amount of isomalto-oligosaccharide exists in certain fermented foods such as soy sauce and yellow wine, and can promote the bifidobacterium in human body to obviously proliferate, has the characteristics of water-soluble dietary fiber function, low calorific value, caries prevention and the like, so the isomaltooligosaccharide is a functional oligosaccharide with wide application, and the isomaltooligosaccharide products on the market at present mainly comprise intestine nourishing health care products with the function of building up the body.

At present, the problem of overhigh content of glucose, maltose and isomaltose still exists in the domestic isomaltose hypgather production, the application range is limited, the isomaltose hypgather production method is not suitable for being used by diabetics, the energy consumption for producing the isomaltose hypgather is high, the separation effect is poor, the recovery rate is low, and the sugar purity is low.

Disclosure of Invention

Aiming at the defects of the prior art, the invention provides a method for preparing isomaltose hypgather, which solves the problems that the application range is limited, the method is not suitable for patients with diabetes, the energy consumption for producing the isomaltose hypgather is high, the separation effect is poor, the recovery rate is low, and the sugar purity is low.

In order to achieve the purpose, the invention is realized by the following technical scheme: the preparation method of isomaltooligosaccharide comprises the following steps:

s1, preparation of starch: selecting a proper amount of excellent wheat, removing impurities in the wheat, cleaning wheat grains, performing water-dressing modulation, controlling the moisture of the modulated wheat to be 15-19%, conveying the wheat grains to peeling equipment for separating the skin layer, modulating the peeled wheat with water again to enable the total moisture in the wheat to reach 20% -25%, then performing embryo breaking treatment on the wheat, screening after embryo breaking to enable the endosperm and the embryo to be completely separated, and pouring the separated endosperm into a flour mill to be crushed into 80-100-mesh wheat flour;

s2, preparation of a liquefied liquid: adding deionized water into a wheat flour raw material to mix into starch milk, wherein the ratio of the deionized water to the wheat flour is 1:3-1:5, the starch milk is adjusted to have a starch milk concentration of 16-19 degrees Be and a pH value of 5.5-6.0, maltotriose generating enzyme and pullulanase are added, the addition amount of the maltotriose generating enzyme is 6.0 multiplied by 105-9.6 multiplied by 105U per ton of the starch raw material, the addition amount of the pullulanase is 3.0 multiplied by 105-6.0 multiplied by 105ASPU per ton of the starch raw material, after the starch is subjected to heat preservation and saccharification for 36-56 hours, enzyme activity is inactivated at 80-90 ℃ to prepare saccharified liquid, alpha-high temperature amylase is added into the starch slurry with the temperature reduced to 30-40 ℃ according to the ratio of 0.23-0.35L of the alpha-high temperature amylase per ton of the starch, and spray liquefaction is carried out at the temperature of 105-109 ℃ to prepare liquefied liquid with the temperature of 93-97 ℃;

s3, saccharification and glucoside conversion: cooling the liquefied solution to 55-65 ℃, adding fungal enzyme which is 0.04-0.06% of the weight of the wheat flour, preserving the heat for 30-40 h, adding transglycosidase which is 0.3-0.5% of the weight of the wheat flour, and maintaining the temperature for 15-25 h at 50-60 ℃;

s4, decoloring treatment: adjusting the pH value of the feed liquid after enzyme deactivation to 4.5-5.5, adding a flocculating agent, standing at 75-85 ℃, keeping the temperature for 30-40 min, adding 1-3% of active carbon, stirring for 30-40 min, and filtering the feed liquid;

s5, subsequent processing: and (2) carrying out ion exchange on the feed liquid in the S4 by using an ion exchange system, wherein the feeding temperature is 40-50 ℃, the flow rate is 12-15 m3/h, the running time is 90-120 min, strong acid and weak base resin is filled in an ion exchange column to prepare refined sugar liquid, the feed liquid after ion exchange is concentrated to 60-65% in volume and then enters a chromatographic separation system, the chromatographic running pressure is 0.25-0.35 MPa, the temperature is 65-70 ℃, the water consumption ratio is 1: 1.2-1: 1.5, 1.4-1.7 m3 is fed per hour, sugar with the polymerization degree of more than or equal to 3 is collected, and then the isomaltooligosaccharide is prepared by concentrating and drying.

Preferably, in the step 3, the transglucosidase is alpha-glucosidase, and the dosage of the transglucosidase is 0.6-0.8 kg/ton based on dry weight of starch.

Preferably, the injection liquefaction in the step 2 is two injection liquefaction, the temperature of the first injection liquefaction is 100-110, and the temperature of the second injection liquefaction is 130-140.

Preferably, the filtration in the step 4 adopts plate-and-frame filtration, the filtration pressure is 0.35Mpa, and the water flow is 5.5-6.5 t/h.

Preferably, the concentration in the step 5 is heating evaporation concentration until the mass concentration of the isomaltooligosaccharide is 50-70% and the pH is 4.2-5.8.

Preferably, the drying in step 5 is drying by a spray drying tower or a vacuum drying device.

The invention provides a preparation method of isomaltose hypgather. Compared with the prior art, the method has the following beneficial effects: the preparation method of the isomaltooligosaccharide comprises the following steps: s1, preparation of starch: selecting a proper amount of excellent wheat, removing impurities in the wheat, cleaning wheat grains, performing water-dressing modulation, controlling the moisture of the modulated wheat to be 15-19%, then conveying the wheat grains into peeling equipment for separation of a skin layer, modulating the peeled wheat again with water to enable the total moisture in the wheat to reach 20% -25%, then performing embryo breaking treatment on the wheat, screening after embryo breaking to enable endosperm and embryo to be completely separated, pouring the separated endosperm into a flour mill, and crushing the endosperm into 80-100 meshes of wheat flour, S2, preparing a liquefied liquid: adding deionized water into a wheat flour raw material to mix into starch milk, wherein the ratio of the deionized water to the wheat flour is 1:3-1:5, the starch milk is adjusted to have a starch milk concentration of 16-19 degrees Be and a pH value of 5.5-6.0, maltotriose generating enzyme and pullulanase are added, the addition amount of the maltotriose generating enzyme is 6.0 multiplied by 105-9.6 multiplied by 105U per ton of the starch raw material, the addition amount of the pullulanase is 3.0 multiplied by 105-6.0 multiplied by 105ASPU per ton of the starch raw material, after the heat preservation and saccharification is carried out for 36-56 hours, enzyme activity is inactivated at 80-90 ℃ to prepare a saccharification liquid, adding alpha-high temperature amylase into the slurry with the temperature reduced to 30-40 ℃ according to the ratio of 0.23-0.35L of the alpha-high temperature amylase per ton of the starch, carrying out spray liquefaction at the temperature of 105-109 ℃ to prepare a liquefaction liquid with the temperature of 93-97 ℃, S3, and saccharified glycoside conversion: cooling the liquefied liquid to 55-65 ℃, adding fungal enzyme which is 0.04-0.06% of the weight of the wheat flour, preserving the heat for 30-40 h, adding transglycosidase which is 0.3-0.5%, maintaining the temperature for 15-25 h at 50-60 ℃, S4, and carrying out decoloration treatment: adjusting the pH value of the feed liquid after enzyme deactivation to 4.5-5.5, adding a flocculating agent, standing at 75-85 ℃, keeping the temperature for 30-40 min, adding 1-3% of active carbon, stirring for 30-40 min, filtering the feed liquid, and S5, performing subsequent treatment: the method comprises the steps of carrying out ion exchange on feed liquid in S4 through an ion exchange system, enabling the feeding temperature to be 40-50 ℃, the flow rate to be 12-15 m3/h, enabling the running time to be 90-120 min, filling strong acid and weak base resin in an ion exchange column to prepare refined sugar liquid, concentrating the feed liquid after ion exchange until the volume is 60-65%, enabling the feed liquid to enter a chromatographic separation system, enabling the chromatographic running pressure to be 0.25-0.35 MPa, the temperature to be 65-70 ℃, the water consumption ratio to be 1: 1.2-1: 1.5, feeding 1.4-1.7 m3 per hour, collecting sugar with the polymerization degree being more than or equal to 3, concentrating and drying to prepare the isomaltooligosaccharide, enabling the purity to be higher than 95% through trisaccharide, enabling the viscosity of syrup to be small, enabling the sweetness to be high, simultaneously increasing the secondary injection liquefaction temperature, ensuring full liquefaction of the feed liquid, improving the protein flocculation effect, being beneficial to the saccharification reaction of the next step, enabling the water-, the nutritional value of the isomaltooligosaccharide is improved without affecting the quality of the isomaltooligosaccharide product, the application range of the isomaltooligosaccharide is expanded, and the final product added with the isomaltooligosaccharide has higher market competitiveness.

Drawings

FIG. 1 is a process flow diagram of the present invention.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Referring to fig. 1, the embodiment of the present invention provides three technical solutions: the preparation method of isomaltooligosaccharide specifically comprises the following steps: