阅读说明：本技术 元宝枫籽油在用于制备改善肠道菌群药物中的应用 (Application of acer truncatum seed oil in preparing medicine for improving intestinal flora ) 是由 陈显扬 宋王婷 韩佳睿 薛腾 于 2020-08-14 设计创作，主要内容包括：本发明提供了元宝枫籽油在用于制备改善肠道菌群药物中的应用。改善肠道菌群的药物,其包括元宝枫籽油在药学上可接受的载体。食用元宝枫籽油或含元宝枫籽油的药物可有效改善肠道菌群,并促进机体对神经酸的吸收。(The invention provides application of acer truncatum seed oil in preparing a medicine for improving intestinal flora. A medicine for improving intestinal flora comprises acer truncatum seed oil and a pharmaceutically acceptable carrier. The edible acer truncatum seed oil or the medicine containing the acer truncatum seed oil can effectively improve the intestinal flora and promote the absorption of the organism to nervonic acid.)

1. Application of Acer truncatum seed oil in preparing medicine for improving intestinal flora is provided.

2. The use of claim 1, wherein the medicament is acer truncatum seed oil in a pharmaceutically acceptable carrier.

3. The use according to claim 1, wherein the medicament is an injection, a sachet, a tablet, an oral liquid or a granule.

4. A medicine for improving intestinal flora is characterized by comprising acer truncatum seed oil and a pharmaceutically acceptable carrier.

5. The pharmaceutical composition of claim 4, wherein the pharmaceutical composition is in the form of an injection, a sachet, a tablet, an oral liquid or a granule.

6. The medicament as claimed in claim 5, wherein the capsule or oral liquid contains Acer Truncatum Bunge seed oil, the capsule can be soft capsule or enteric capsule, the oral liquid contains Acer Truncatum Bunge seed oil and sucrose, and the sucrose content is more than 60%.

7. The use of claim 1, wherein the acer truncatum seed oil comprises, by weight, 3.9 to 4.02 parts of palmitic acid, 0.05 to 0.09 parts of palmitoleic acid, 0.05 to 0.09 parts of heptadecacarbonic acid, 0.03 to 0.06 parts of cis-10-heptadecaenoic acid, 2.28 to 2.68 parts of stearic acid, 20 to 23 parts of oleic acid, 28 to 32 parts of linoleic acid, 0.20 to 0.32 parts of arachidic acid, 0.54 to 0.9 parts of gamma-linolenic acid, 8.0 to 8.8 parts of cis-11-eicosenoic acid, 1 to 2 parts of alpha-linolenic acid, 0.2 to 0.4 parts of cis-11, 14-eicosadienoic acid, 0.96 parts of behenic acid, 18.7 parts of erucic acid, 18.7 parts of cis-11, 14, 17-eicosatrienoic acid, 0.1 to 0.2 parts of tricosanoic acid, 0.02 to 0.06 parts of tricosanoic acid, 0.01 to 13 parts of docosadienoic acid, 0.01 to 0.7 parts of tetracosenoic acid, and 0.05 to 15 parts of tetracosenoic acid.

Technical Field

The invention belongs to the technical field of biology, and particularly relates to application of acer truncatum seed oil in preparation of a medicine for improving intestinal flora.

Background

The acer truncatum seed oil is a vegetable oil processed from seeds of acer truncatum which is a tree species in China, the content of unsaturated fatty acid reaches 92 percent, 3.2011, 22 days, No. 9 document of Ministry of health of the people's republic of China approves the acer truncatum seed oil as a new resource food, and the acer truncatum seed oil formally enters the ranks of edible vegetable oil in China.

Acer truncatum seed oil is a unique resource in China, and 5% -6% of the Acer truncatum seed oil is nervonic acid. Nervonic acid has been discovered for more than 90 years, and the physiological function of nervonic acid is already clear and is important active fatty acid in human bodies. The research of the plant nervonic acid in China is late, but the research has been in direct pursuit in the last two years. Acer truncatum seed oil is taken as a vegetable oil resource rich in nervonic acid, and has recently received attention of relevant scholars and enterprises.

However, can acer truncatum seed oil be supplemented without interfering with intestinal flora? What changes do intestinal flora metabolites? These very important problems have not been clearly studied to date and even few have been demonstrated. Therefore, the invention evaluates the influence of acer truncatum seed oil on intestinal flora and can be used for improving the development and application of intestinal flora medicaments in the future.

Disclosure of Invention

In order to solve the technical problems, the invention provides application of acer truncatum seed oil in preparing a medicine for improving intestinal flora.

In order to achieve the purpose, the invention adopts the following technical scheme that:

application of Acer truncatum seed oil in preparing medicine for improving intestinal flora is provided. The above-mentioned application, preferably, the medicine is acer truncatum seed oil in a pharmaceutically acceptable carrier.

As mentioned above, the medicament can be prepared into various dosage forms, including injection, capsule, tablet, oral liquid or granule.

A medicine for improving intestinal flora comprises Acer Truncatum Bunge seed oil in pharmaceutically acceptable carrier.

The medicament as described above, preferably, the medicament is an injection, a sachet, a tablet, an oral liquid or a granule.

Preferably, the capsule or the oral liquid contains acer truncatum seed oil, the capsule can be a soft capsule or an enteric capsule, and the oral liquid contains the acer truncatum seed oil and sucrose with the amount of more than 60%.

Preferably, the acer truncatum seed oil comprises, by weight, 3.9-4.02 parts of palmitic acid, 0.05-0.09 part of palmitoleic acid, 0.05-0.09 part of heptadecacarbonic acid, 0.03-0.06 part of cis-10-heptadecaenoic acid, 2.28-2.68 parts of stearic acid, 20-23 parts of oleic acid, 28-32 parts of linoleic acid, 0.20-0.32 part of arachidic acid, 0.54-0.9 part of gamma-linolenic acid, 8.0-8.8 parts of cis-11-eicosenoic acid, 1-2 parts of alpha-linolenic acid, 0.2-cis-11, 14-eicosadienoic acid, 0.96 part of tribehoic acid, 18.7 parts of erucic acid, 0.1-0.2 part of cis-11, 14, 17-eicosatrienoic acid, 0.02-0.06 part of triconic acid, 0.13 part of cis-13, 0.01-docosadienoic acid, 0.03-0.5-tetracosanoic acid (0.05-2 parts of tetracosanoic acid).

After taking Acer truncatum seed oil, intestinal flora can be improved to intervene neuroinflammation.

The invention has the beneficial effects that:

the invention provides an application of acer truncatum seed oil in preparing a medicine for improving intestinal flora.

Experimental research shows that the edible acer truncatum seed oil can effectively improve intestinal flora and promote the absorption of nervonic acid by organisms. Meanwhile, microorganisms related to neuroprotection are obviously increased, and microorganisms related to proinflammatory type are obviously reduced, which shows that the neuroinflammation can be intervened by improving the intestinal flora after taking acer truncatum buge oil. After people take acer truncatum seed oil, the lactobacillus is increased, and the ratio of firmicutes to bacteroidetes is reduced, which shows that the acer truncatum seed oil can adjust the dynamic balance of intestinal flora and well improve or reshape the flora.

Drawings

FIG. 1 is a butterfly diagram showing the change in the level of intestinal flora in rats after 7 days of administration of acer truncatum seed oil;

FIG. 2 is a butterfly diagram showing the change in the level of intestinal flora in rats after 7 days of administration of acer truncatum seed oil;

FIG. 3 is a ROC plot in a logistic regression model;

FIG. 4 is a compound with VIP >1 in positive and negative ion mode 7 days after acer truncatum seed oil supplementation;

FIG. 5 is a graph of the scores of (O) PLS-DA in positive and negative ion mode 7 days after supplementation with Acer truncatum seed oil;

FIG. 6 is a S-plot in positive and negative ion mode 7 days after the Acer truncatum seed oil is supplemented;

FIG. 7 is a schematic of the significant changes in lipids in feces after taking Acer truncatum oil;

FIG. 8 ROC plots in a logistic regression model;

FIG. 9 is a butterfly diagram of the change in the level of gut flora after 7 days of administration of acer truncatum seed oil;

FIG. 10 is a butterfly diagram of the change in the level of gut flora after 7 days of human consumption of acer truncatum seed oil.

Detailed Description

The following examples are intended to further illustrate the invention but should not be construed as limiting it. Modifications and substitutions may be made thereto without departing from the spirit and scope of the invention.

The experimental apparatus and reagents used in the examples of the present invention: 1. freezing a centrifuge: model D3024R, Scilogex corporation, usa; 2. a vortex oscillator: model MX-S, Scilogex, USA; 3. high resolution mass spectrometer: ESI-QTOF/MS; the model is as follows: xevo G2-S Q-TOF; the manufacturer: waters, Manchester, UK; 4. ultra-high performance liquid chromatography: UPLC; the model is as follows: the ACQUITY UPLC I-Class system; the manufacturer: waters, Manchester, UK; 5. data acquisition software: MassLynx4.1; the manufacturer: waters; 6. analysis and identification software: prognesis QI; the manufacturer: waters;

experimental reagent: isopropanol, acetonitrile, formic acid, ammonium formate, leucine enkephalin and sodium formate. The manufacturers are Fisher. The acer truncatum seed oil used in the following examples of the present invention was provided by acer truncatum biotechnology (beijing) ltd, the components are detected by a third party detection company, namely Banni, and the detection result comprises, by weight, 4.02 parts of palmitic acid, 0.07 part of palmitoleic acid, 0.07 part of heptadecacarbonic acid, 0.04 part of cis-10-heptadecaenoic acid, 2.48 parts of stearic acid, 21.8 parts of oleic acid, 30.7 parts of linoleic acid, 0.28 part of arachidic acid, 0.74 part of gamma-linolenic acid, 8.54 parts of cis-11-eicosenoic acid, 1.65 parts of alpha-linolenic acid, 0.34 part of cis-11, 14-eicosadienoic acid, 0.96 part of behenic acid, 18.7 parts of erucic acid, 0.16 part of cis-11, 14, 17-eicosatrienoic acid, 0.04 part of tricosanoic acid, 0.02 part of cis-13, 16-docosadienoic acid, 0.42 part of tetracosanoic acid and 6.89 parts of cis-15-tetracosanoic acid (nervonic acid). Unless otherwise specified, the technical means used in the examples are conventional means well known to those skilled in the art.