阅读说明：本技术 一种简便快速去除三七总皂苷中蛋白质的方法 (Method for simply, conveniently and rapidly removing proteins in panax notoginseng saponins ) 是由 龚云麒 黄茜 高宏涛 于 2019-03-20 设计创作，主要内容包括：本发明公开了一种简便快速去除三七总皂苷中蛋白质的方法。所述的简便快速去除三七总皂苷中蛋白质的方法是在三七总皂苷中加入5~10倍的溶剂溶解得到三七总皂苷溶液,在10~30℃下过离子纤维素柱以乙醇容易洗脱,收集合并洗脱液,减压浓缩干燥得到目标物去蛋白三七总皂苷。本发明是将三七总皂苷溶解于溶剂中,在室温条件下,快速通过离子纤维素,使蛋白质吸附于离子纤维素。本发明具有快速、高效,操作简便等优势,具有较高的应用价值和开发前景。(The invention discloses a method for simply, conveniently and quickly removing proteins in panax notoginseng saponins. The method for simply, conveniently and quickly removing the protein in the panax notoginseng saponins comprises the steps of adding 5-10 times of solvent into the panax notoginseng saponins to dissolve the panax notoginseng saponins to obtain panax notoginseng saponins solution, passing the panax notoginseng saponins solution through an ion cellulose column at 10-30 ℃ to easily elute by ethanol, collecting and combining eluent, and concentrating and drying under reduced pressure to obtain the target deproteinized panax notoginseng saponins. The invention dissolves the panax notoginseng saponins in a solvent, and the panax notoginseng saponins rapidly pass through the ionic cellulose at room temperature so as to lead the protein to be adsorbed on the ionic cellulose. The method has the advantages of rapidness, high efficiency, simple and convenient operation and the like, and has higher application value and development prospect.)

1. A method for simply, conveniently and quickly removing proteins in panax notoginseng saponins is characterized in that a solvent which is 5-10 times of the total panax notoginseng saponins is added into the total panax notoginseng saponins for dissolution to obtain a panax notoginseng saponins solution, the total panax notoginseng saponins solution is easily eluted by ethanol through an ion cellulose column at 10-30 ℃, elution solutions are collected and combined, and the target deproteinized panax notoginseng saponins are obtained by reduced pressure concentration and drying.

2. The method for easily and rapidly removing Panax notoginsenosides protein according to claim 1, wherein the solvent is water and/or alcohol.

3. The method for simply, conveniently and rapidly removing panax notoginseng saponins protein according to claim 2, characterized in that the alcohol is an alcohol with 1-3 carbon atoms.

4. The method for simply and rapidly removing Panax notoginsenosides protein in claim 1, wherein the dissolution is heating with stirring.

5. The method for simply, conveniently and rapidly removing panax notoginseng saponins protein according to claim 4, wherein the heating is carried out at a heating rate of 2-3 ℃/min to 70-80 ℃.

6. The method for easily and rapidly removing Panax notoginsenosides protein according to claim 1, further comprising a cooling step after dissolution.

7. The method for simply, conveniently and rapidly removing panax notoginseng saponins protein according to claim 6, wherein the cooling is performed at a cooling rate of 5-8 ℃/min to 10-30 ℃.

8. The method for simply and rapidly removing panax notoginseng saponins protein according to claim 1, characterized in that the ionic cellulose is cationic cellulose.

9. The method for simply, conveniently and rapidly removing panax notoginseng saponins protein according to claim 1 or 8, characterized in that the amount of the ionic cellulose is 1/10-1/20 of the weight of panax notoginseng saponins.

10. The method of claim 1, wherein the ionic cellulose column is prepared by dissolving and loading 2g of cationic cellulose in 20ml of deionized water, washing with 20ml of 0.1mol/L NaOH solution, adding water to neutralize, washing with 0.1mol/L HCl20ml, adding water to neutralize, and storing.

Technical Field

The invention belongs to the technical field of deep processing of medicines, and particularly relates to a method for simply, conveniently and quickly removing proteins in panax notoginseng saponins.

Background

Notoginseng radix is Araliaceae perennial herbPanax notoginseng(Burk)Dried root of Chen, mainly produced in Yunnan, Guangxi, etc. Is one of the genuine medicinal materials in Yunnan province and has the name of 'the king of the ginseng'. Is a wide applicationThe Chinese medicinal composition has the advantages of good curative effect, well-known effects of stopping bleeding, removing blood stasis, relieving swelling and pain, enriching blood, activating blood circulation, clearing heat, calming the liver and reducing blood pressure, has certain nourishing and strengthening effects, and is popular with patients.

At present, the panax notoginseng products produced nationwide reach three hundred and more than three kinds, wherein the preparation development of panax notoginseng saponins is more successful, in particular to panax notoginseng saponins injections (Xuesaitong injection, Xuesaitong for injection, Xueshitong injection and Xueshitong for injection), which become one of ten Chinese medicine varieties for treating cardiovascular and cerebrovascular diseases.

Panax notoginsenosides (hereinafter referred to as PNS) are saponin components extracted from the rhizome of Panax notoginseng, have pharmacological effects of dilating blood vessels, inhibiting platelet aggregation, prolonging blood coagulation time, reducing blood lipid, scavenging free radicals, resisting inflammation, resisting oxidation, etc., and are mainly used for treating cardiovascular and cerebrovascular diseases clinically. The research on the extraction and purification of the panax notoginseng saponins in the panax notoginseng has been performed by removing pigments and small molecular impurities, but neglecting the removal of trace protein substances in the panax notoginseng saponins. In addition, the current pharmacopoeia method for testing injection for thromboembolism uses sulfosalicylic acid method for testing, which is based on the principle that protein is positively charged in acidic environment, and sulfosalicylate is negatively charged, and it is combined with protein to precipitate to show the presence of protein. This method is difficult to accurately detect a trace amount of protein.

The anaphylactic reaction of the traditional Chinese medicine injection is the most common, and the residual vegetable protein in the traditional Chinese medicine injection is one of the main reasons for the anaphylactic reaction. Through intensive research, a method for quickly and efficiently removing trace protein in panax notoginseng saponins is found on the basis of the existing research, and has obvious comprehensive advantages compared with the traditional extraction method.

Disclosure of Invention

The invention aims to provide a method for simply, conveniently and quickly removing proteins in panax notoginseng saponins.

The method for simply, conveniently and quickly removing the protein in the panax notoginseng saponins is to add 5-10 times of solvent into the panax notoginseng saponins to dissolve the panax notoginseng saponins to obtain panax notoginseng saponins solution, pass through an ion cellulose column at 10-30 ℃ to easily elute by ethanol, collect and combine eluates, and perform reduced pressure concentration and drying to obtain the target deproteinized panax notoginseng saponins.

The invention dissolves the panax notoginseng saponins in a solvent, and the panax notoginseng saponins rapidly pass through the ionic cellulose at room temperature so as to lead the protein to be adsorbed on the ionic cellulose. The method has the advantages of rapidness, high efficiency, simple and convenient operation and the like, and has higher application value and development prospect.

Drawings

FIG. 1 is a graph showing a standard curve of protein content.

Detailed Description

The present invention is further illustrated by the following examples and the accompanying drawings, but the present invention is not limited thereto in any way, and any modifications or alterations based on the teaching of the present invention are within the scope of the present invention.

The method for simply, conveniently and quickly removing the protein in the panax notoginseng saponins comprises the steps of adding 5-10 times of solvent into the panax notoginseng saponins to dissolve the panax notoginseng saponins to obtain panax notoginseng saponins solution, passing the panax notoginseng saponins solution through an ion cellulose column at 10-30 ℃ to easily elute by ethanol, collecting and combining eluent, and concentrating and drying under reduced pressure to obtain the target deproteinized panax notoginseng saponins.

The solvent is water and/or alcohol.

The alcohol is alcohol with 1-3 carbon atoms.

The dissolving is heating and stirring.

The heating is carried out at a heating rate of 2-3 ℃/min to 70-80 ℃.

A cooling step is also included after dissolution.

The cooling is carried out at a cooling rate of 5-8 ℃/min to 10-30 ℃.

The ionic cellulose is cationic cellulose.

The dosage of the ionic cellulose is 1/10-1/20 of the weight of the panax notoginseng saponins.

The preparation method of the ionic cellulose column comprises the steps of taking 2g of cationic cellulose, adding 20ml of deionized water, loading the cationic cellulose after dissolution and dispersion into the column, firstly adding 20ml of 0.1mol/L NaOH solution for washing, adding water for washing until the solution is neutral, then adding 0.1mol/L HCl20ml for washing, adding water for washing until the solution is neutral, and storing for later use.

The invention is further illustrated by the following specific examples: