阅读说明：本技术 一种紫锥菊提取物及其制备方法 (Echinacea purpurea extract and preparation method thereof ) 是由 詹晓峰 冯涛 周岩 于 2020-07-20 设计创作，主要内容包括：本发明涉及一种紫锥菊提取物及其制备方法,属于医药技术领域。于每年8月中旬至9月末采收鲜紫锥菊,切制成5-15cm的段,连续逆流提取器先加入90-98℃热水,再投入切制鲜紫锥菊进行灭酶；调节pH值4.2-6.2,喷淋提取60-90min,得到提取液；再将提取液过D941大孔弱碱阴离子交换树脂(每个树脂柱装330-780kg树脂),收集走柱液；走柱液减压浓缩至相对密度1.12-1.18,加入2.5-3.2倍量体积的95％乙醇,得到醇沉清液后继续减压浓缩回收乙醇,得到浸膏；浸膏喷雾干燥得紫锥菊提取物。采用本发明的提取方法,在较短的时间内,完成了原料的采收、连续提取、调节HP值、树脂纯化等技术,提高菊苣酸含量至4％-12％。规模化生产,降低了紫锥菊提取物成本,有利于市场化。(The invention relates to an echinacea purpurea extract and a preparation method thereof, belonging to the technical field of medicines. Collecting fresh Echinacea purpurea from 8 middle ten days to 9 end months per year, cutting into 5-15cm segments, adding 90-98 deg.C hot water into continuous countercurrent extractor, and inactivating enzyme; adjusting pH to 4.2-6.2, and spray extracting for 60-90min to obtain extractive solution; then passing the extract through D941 macroporous weak base anion exchange resin (330-780 kg resin is loaded in each resin column), and collecting column liquid; concentrating the column solution under reduced pressure to relative density of 1.12-1.18, adding 2.5-3.2 times volume of 95% ethanol to obtain ethanol precipitation clear liquid, and concentrating under reduced pressure to recover ethanol to obtain extract; spray drying the extract to obtain the echinacea purpurea extract. By adopting the extraction method, the technologies of raw material collection, continuous extraction, HP value adjustment, resin purification and the like are completed in a short time, and the content of chicoric acid is increased to 4-12%. The large-scale production reduces the cost of the echinacea extract and is beneficial to marketization.)

1. A method for preparing Echinacea purpurea extract comprises:

(1) cutting into sections: picking fresh echinacea purpurea and cutting into sections;

(2) enzyme deactivation: heating water in a continuous countercurrent extractor to inactivate enzyme in Echinacea purpurea segment;

(3) preparing an extracting solution: adjusting the pH value, and spraying and extracting to obtain an extracting solution;

(4) collecting and pouring liquid: passing the extract through D941 macroporous weak base anion exchange resin, and collecting the column-passing solution;

(5) preparing alcohol precipitation clear liquid: concentrating the column solution under reduced pressure, and adding 2.5-3.2 times volume amount of 95% ethanol to obtain ethanol precipitation clear solution;

(6) concentrating the clear liquid under reduced pressure, and recovering ethanol to obtain extract;

(7) spray drying the extract to obtain the echinacea purpurea extract.

2. The production method according to claim 1, wherein:

picking time in the step (1) is from 8 middle ten days to 9 months end every year; the cut-to-length is 5-15 cm.

3. The production method according to claim 1, wherein:

the temperature of the hot water in the step (2) is 90-98 ℃.

4. The production method according to claim 1, wherein:

adjusting the pH value to 4.2-6.2 in the step (3), and spraying and extracting for 60-90 min.

5. The production method according to claim 1, wherein:

the column packing amount of the resin in the step (4) is 330-780 kg/column.

6. The production method according to claim 1, wherein:

and (5) concentrating the column-running liquid under reduced pressure until the relative density is 1.12-1.18.

7. An echinacea extract prepared by the method of any one of claims 1-6.

8. Echinacea extract according to claim 7, wherein the chicoric acid content is between 4% and 12%.

Technical Field

The invention belongs to the technical field of medicines, relates to a traditional Chinese medicine extract, and particularly relates to a preparation method of an echinacea extract and the echinacea extract prepared by the method.

Background

The echinacea extract is derived from echinacea plants, can obviously improve the immunoregulation level of organisms by being used as an immune promoter and a regulator, and is mainly used for treating various bacterial infection symptoms such as cold (upper respiratory infection), typhoid and the like. In 1989, the rank of the 2000 most commonly used prescriptions was 131 in germany. In 1982, the types of single and compound echinacea preparations are as many as 300, and a plurality of dosage forms are used in clinic in a large number, such as treating acute and chronic respiratory tract infection caused by virus or bacteria, assisting various antibiotics to treat various serious bacterial infections, and the like. The echinacea plant is native to the North America region, and at present, the echinacea is successfully introduced in partial regions of China, so that the problem of raw materials is solved, and the research on chemical components and the activity of the domestic echinacea extract is also ongoing, so that a necessary foundation is laid for the development of the domestic echinacea extract. Today in the 21 st century, the health care concept of people is shifted from single treatment of diseases to enhancement of autoimmunity improvement, and prevention is mainly. With the increase of various chronic diseases, senile diseases and other difficult and complicated diseases and the awareness and importance of people on health, a good development space is brought to the development of novel medicinal materials and raw material medicines.

One of the active ingredients of Echinacea purpurea extract is polyphenol substance represented by chicoric acid, however, the existence of polyphenol oxidase which can catalyze and decompose polyphenol active ingredients in Echinacea purpurea plant can lead to gradually reducing the content of chicoric acid in the process of extracting and processing Echinacea purpurea extract, which is not good for developing new product.

Based on the analysis, the preparation method of the echinacea extract for preventing the chicoric acid which is an effective component from decomposing and improving the content of the effective component so that the content of the chicoric acid reaches 4 to 12 percent in large-scale production and the echinacea extract prepared by the method are urgently needed by the industry at present.

Disclosure of Invention

In view of the defects, the invention provides a preparation method of an echinacea extract, which can prevent the chicoric acid serving as an effective component from being decomposed, improve the content of the effective component and enable the content of the chicoric acid to reach 4-12% in large-scale production, and the echinacea extract prepared by the method. The invention is realized by the following means:

a method for preparing Echinacea purpurea extract comprises:

(1) collecting fresh Echinacea purpurea from 8 middle ten days to 9 end of month, and cutting into 5-15cm segments;

(2) adding hot water of 90-98 deg.C into continuous countercurrent extractor, and adding cut fresh purple coneflower for inactivating enzyme;

(3) adjusting pH to 4.2-6.2, and spray extracting for 60-90min to obtain extractive solution;

(4) passing the extract through D941 macroporous weak base anion exchange resin with the loading of resin column of 330 and 780 kg/column, and collecting column liquid;

(5) concentrating the column solution under reduced pressure to relative density of 1.12-1.18, adding 2.5-3.2 times volume of 95% ethanol to obtain alcohol precipitation clear solution;

(6) concentrating the clear liquid under reduced pressure, and recovering ethanol to obtain extract;

(7) spray drying the extract to obtain the echinacea purpurea extract, wherein the content of the chicoric acid is 4-12 percent.

The invention has the beneficial effects that:

1. the invention aims to provide a method for converting large-batch planted echinacea purpurea into a medicinal raw material with immunoregulatory activity, which is simple, efficient and low in cost.

2. The process can improve the defect of low content of chicoric acid in the traditional echinacea purpurea extract, achieves the effect of improving the content of the active ingredients of the chicoric acid in the echinacea purpurea extract to 4-12 percent through close cooperation of the processes, and can realize large-scale production, reduce the cost and increase the benefit.

Detailed Description