阅读说明：本技术 中药川乌的炮制方法 (Processing method of traditional Chinese medicine radix aconiti ) 是由 钟凌云 杨明 龚千锋 叶协涛 祝婧 叶喜德 黄艺 于欢 王芳 范晖 于 2020-08-10 设计创作，主要内容包括：本发明涉及一种一种中药川乌的炮制方法,其包括如下步骤：S1:取净川乌,在水中浸泡至内无干心；S2:取出晾干后,加入生姜汁拌匀,闷润至润透；S3:加水淹没川乌,隔水蒸至内无白心,口尝带麻舌感；S4:取出,晾干切薄片,干燥。与现有常规炮制方法相比,本发明的中药川乌的炮制方法,具有更加确切的工艺参数,更定量化的控制条件,便于指导实际生产,以获得质量、药效性能稳定的制川乌熟品。经本发明的工艺炮制川乌后,双酯型乌头碱(毒性成分)含量为0.00774%,为生品含量的8.1%。单酯型乌头碱(药效成分)含量为0.08452%,约为生品含量1.9倍。醇胺型乌头碱含量为0.05806%,约为生品含量的1.2倍；与传统炮制川乌相比还部分保留了川乌中的抗炎药效成分准葛尔乌头胺的含量。(The invention relates to a processing method of traditional Chinese medicine radix aconiti, which comprises the following steps: s1, soaking the cleaned radix Aconiti in water until there is no dry core; s2, taking out, air drying, adding ginger juice, stirring, and moistening; s3, adding water to submerge the radix aconiti, steaming the radix aconiti over water until no white heart exists, and tasting the radix aconiti with a numb tongue feeling; s4, taking out, airing, slicing and drying. Compared with the conventional processing method, the processing method of the traditional Chinese medicine radix aconiti has more exact process parameters and more quantitative control conditions, is convenient for guiding actual production, and obtains the prepared radix aconiti preparata with stable quality and pharmacodynamic performance. After the monkshood is processed by the process, the content of diester aconitine (toxic component) is 0.00774 percent, which is 8.1 percent of the content of the product. The content of monoester aconitine (effective component) is 0.08452%, which is about 1.9 times of the content of raw product. The content of the hydramine type aconitine is 0.05806 percent and is about 1.2 times of the content of the raw product; compared with the traditional processed radix aconiti, the content of the anti-inflammatory drug effect component pseudo-puerariae aconitine in the radix aconiti is partially reserved.)

1. A processing method of a traditional Chinese medicine radix aconiti is characterized by comprising the following steps:

s1, soaking the cleaned radix Aconiti in water until there is no dry core;

s2, taking out, air drying, adding ginger juice, stirring, and moistening;

s3, adding water to submerge the radix aconiti, steaming the radix aconiti over water until no white heart exists, and tasting the radix aconiti with a numb tongue feeling;

s4, taking out, airing, slicing and drying.

2. The processing method as claimed in claim 1, wherein in S1, radix Aconiti is soaked for 40-60h, and water is changed every 10-12h until there is no dry core.

3. The processing method of claim 2, wherein in S1, when the soaking time is 48h, the monkshood can be cut off with scissors or broken off with hands, to check whether there is any dry heart, if there is any dry heart, the soaking time is further prolonged, but the total soaking time is not longer than 60 h.

4. The processing method of claim 1, wherein the ginger juice is added to S2 in an amount that: preparing ginger juice by using 25g of fresh ginger per 100g of radix aconiti;

the preparation method of the ginger juice comprises the following steps: cleaning rhizoma Zingiberis recens, slicing, adding water 15-20% of rhizoma Zingiberis recens, squeezing for 4 times, adding 10-15% of water each time, filtering, rotary concentrating at low temperature, and diluting to a constant volume of 1.0-2 g/mL.

5. The processing method of claim 1, wherein in step S3, steaming is carried out for 7-8h until the taste has a slightly numb tongue.

6. The processing method as claimed in claim 1, wherein in S4, drying is carried out until weight loss is 40-60%, slicing and drying.

7. The application of ginger in reducing the toxicity of radix aconiti and improving the drug effect in the processing process of the radix aconiti; the processing method is characterized by comprising the following steps:

s1, soaking the cleaned radix Aconiti in water until there is no dry core;

s2, taking out, air drying, adding ginger juice, stirring, and moistening;

s3, adding water to submerge the radix aconiti, steaming the radix aconiti over water until no white heart exists, and tasting the radix aconiti with a numb tongue feeling;

s4, taking out, airing, slicing and drying;

by the processing method, the content of the drug effect component monoester type aconitine in the radix aconiti is 1.9 times of the content of the product, the content of the alcohol amine type aconitine is 1.2 times of the content of the product, and the content of the toxic component diester type aconitine is 8.1 percent of the content of the product; the radix aconiti also contains an anti-inflammatory drug effect component of pseudo-pueraria mirifica amine.

8. The use according to claim 7, wherein in S2, the ginger juice is added in an amount of: preparing ginger juice by using 25g of fresh ginger per 100g of radix aconiti;

the preparation method of the ginger juice comprises the following steps: cleaning rhizoma Zingiberis recens, slicing, adding water 15-20% of rhizoma Zingiberis recens, squeezing for 4 times, adding 10-15% of water each time, filtering, rotary concentrating at low temperature, and diluting to a constant volume of 1.0-2 g/mL.

9. The use according to claim 7, wherein in S3, steaming is carried out for 7-8h until a light tingling sensation is tasted.

10. The processed radix aconiti is characterized in that the content of diester aconitine is 0.00771-0.00778%; the content of monoester aconitine is 0.08450-0.08455%; the content of hydramine type aconitine is 0.05802-0.05810%; the content of the pseudo-aconitine is more than 0.

Technical Field

The invention belongs to the technical field of traditional Chinese medicine processing, and particularly relates to a processing method of traditional Chinese medicine monkshood.

Background

Radix Aconiti is dry mother root, hot, pungent and bitter of Aconitum carmichaeli (L.) nakai) Carmichaelii (Ranunculaceae), belongs to interior-warming medicine, and has pharmacological effects of dispelling pathogenic wind, removing dampness, dispelling cold, relieving pain, warming channels and removing blood stasis. Since ancient times, Sichuan aconite root is highly appreciated by doctors because of its remarkable curative effects on cold hernia, fullness and pain in chest and hypochondrium, and blood and qi deficiency. However, unprocessed Chuan Wu is toxic and usually needs to be processed before being used as a medicine in clinical application.

The processing method of radix aconiti recorded in 'Chinese pharmacopoeia' of 2015 edition comprises the following steps: separating radix Aconiti from small one, soaking in water until there is no dry core, taking out, steaming for 6-8 hr until large one and solid one have no white core, and have slight tongue and numbness, taking out, air drying to six degrees, slicing, and drying.

As early as in the Han Dynasty medical prescription, the processing method of Sichuan aconite root is recorded in the fifty-two prescriptions. In the pharmacopoeia processing method, the soaking time and the steaming time are not specified by exact parameters and are judged by experience, so that the situations of incomplete cooking or excessive cooking are easy to occur. Meanwhile, most modern processing methods of radix aconiti refer to the recording method of pharmacopoeia, and no auxiliary materials are added in the processing process.

Modern researches show that the ginger can increase the dissolution of effective components of the traditional Chinese medicine of the monkshood and improve the drug effect. However, the processing methods described in the related research documents have a lot of ambiguity, and are not suitable for practical production.

Disclosure of Invention

Technical problem to be solved

In view of the above disadvantages of the prior art, the invention provides a processing method of traditional Chinese medicine radix aconiti, which is used for standardizing the processing technology of traditional Chinese medicine radix aconiti and solves the problems of fuzzy process, strong randomness, low standardization degree and the like of the existing processing method of radix aconiti.

According to the invention, the ginger juice is added in the process of processing the radix aconiti, so that the content of the active ingredients, namely monoester type aconitine and alcohol amine type aconitine, in the processed radix aconiti is obviously improved, the content of the toxic ingredient, namely diester type aconitine is obviously reduced, the anti-inflammatory active ingredient, namely pseudo-puerariae aconitine, in the radix aconiti is also retained, and the medicinal effect value of the processed radix aconiti is improved.

(II) technical scheme

In order to achieve the purpose, the invention adopts the main technical scheme that:

in a first aspect, an embodiment of the present invention provides a processing method of a traditional Chinese medicine monkshood, which includes:

s1, soaking the cleaned radix Aconiti in water until there is no dry core;

s2, taking out, air drying, adding ginger juice, stirring, and moistening;

s3, adding water to submerge the radix aconiti, steaming the radix aconiti over water until no white heart exists, and tasting the radix aconiti with a numb tongue feeling;

s4, taking out, airing, slicing and drying.

According to the preferred embodiment of the present invention, in S1, the Sichuan aconite root is soaked for 40-60h, and water is changed every 10-12h until there is no dry core. When the soaked aconite is fully soaked for 48 hours, the aconite can be cut off by scissors or broken off by hands, whether dried hearts exist or not is checked, if the dried hearts exist, the soaking time is continuously prolonged, but the soaking time is not more than 60 hours.

According to a preferred embodiment of the present invention, in S2, the ginger juice is added in an amount of: preparing ginger juice by using 25g of fresh ginger per 100g of radix aconiti;

the preparation method of the ginger juice comprises the following steps: cleaning rhizoma Zingiberis recens, slicing, adding water 15-20% of rhizoma Zingiberis recens, squeezing for 4 times, adding 10-15% of water each time, filtering, rotary concentrating at low temperature, and diluting to a constant volume of 1.0-2 g/mL.

Adding water accounting for 15-20% of the weight of fresh ginger X g into the fresh ginger, repeatedly squeezing for 4 times, adding 10% -15% of water into the fresh ginger each time, filtering, carrying out low-temperature rotary concentration, and fixing the volume to V ml, wherein V is 1: 1-2; can reach 1-2g ginger per ml water.

According to the preferred embodiment of the present invention, in S3, steaming for 7-8h until a light numb tongue feeling is tasted.

According to the preferred embodiment of the present invention, in S4, drying is carried out until the weight is reduced by 40-60%, and then slicing and drying are carried out.

On the other hand, the invention also provides the application of the ginger in reducing the toxicity of the radix aconiti and improving the drug effect in the processing process of the radix aconiti; the processing method comprises the following steps:

s1, soaking the cleaned radix Aconiti in water until there is no dry core;

s2, taking out, air drying, adding ginger juice, stirring, and moistening;

s3, adding water to submerge the radix aconiti, steaming the radix aconiti over water until no white heart exists, and tasting the radix aconiti with a numb tongue feeling;

s4, taking out, airing, slicing and drying;

by the processing method, the content of the drug effect component monoester type aconitine in the radix aconiti is 1.9 times of the content of the product, the content of the alcohol amine type aconitine is 1.2 times of the content of the product, and the content of the toxic component diester type aconitine is 8.1 percent of the content of the product; the radix aconiti also contains an anti-inflammatory drug effect component of pseudo-pueraria mirifica amine.

According to the preferred embodiment of the present invention, in S1, the Sichuan aconite root is soaked for 40-60h, and water is changed every 10-12h until there is no dry core. When the soaked aconite is fully soaked for 48 hours, the aconite can be cut off by scissors or broken off by hands, whether dried hearts exist or not is checked, if the dried hearts exist, the soaking time is continuously prolonged, but the soaking time is not more than 60 hours.

According to a preferred embodiment of the present invention, in S2, the ginger juice is added in an amount of: preparing ginger juice by using 25g of fresh ginger per 100g of radix aconiti;

the preparation method of the ginger juice comprises the following steps: cleaning rhizoma Zingiberis recens, slicing, adding water 15-20% of rhizoma Zingiberis recens, squeezing for 4 times, adding 10-15% of water each time, filtering, rotary concentrating at low temperature, and diluting to a constant volume of 1.0-2 g/mL.

According to the preferred embodiment of the present invention, in S3, steaming for 7-8h until a light numb tongue feeling is tasted.

According to the preferred embodiment of the present invention, in S4, drying is carried out until the weight is reduced by 40-60%, and then slicing and drying are carried out.

On one hand, the invention provides prepared monkshood, wherein the content of diester-type aconitine is 0.00771-0.00778%; the content of monoester aconitine is 0.08450-0.08455%; the content of hydramine type aconitine is 0.05802-0.05810%; the content of the pseudo-aconitine is more than 0.

(III) advantageous effects

Compared with the conventional processing method, the processing method of the traditional Chinese medicine radix aconiti has more exact process parameters and more quantitative control conditions, is convenient for guiding actual production, and obtains the prepared radix aconiti preparata with stable quality and pharmacodynamic performance.

After the monkshood is processed by the process, the content of diester aconitine (toxic component) is 0.00774 percent, which is 8.1 percent of the content of the product. The content of monoester aconitine (effective component) is 0.08452%, which is about 1.9 times of the content of raw product. The content of the hydramine type aconitine (medicinal component) is 0.05806%, which is about 1.2 times of the raw product content. Meanwhile, compared with the traditional processed radix aconiti, the processed radix aconiti of the invention can partially reserve the content of the sinomontanine (an anti-inflammatory drug effective component) in the radix aconiti.

Drawings

FIG. 1 is a total ion flow diagram of unprocessed product (A1), processed radix Aconiti (A2) in example 1, and processed radix Aconiti (A3) in example 2 under positive ion mode;

FIG. 2 is a total ion flow diagram of the processed product of unprocessed product (B1), prepared Sichuan aconite root (B2) in example 1 and prepared Sichuan aconite root (B3) in example 2 under the negative ion mode;

fig. 3 is a schematic diagram of a computer-simulated structure of eugoniamine and inflammatory proteins MAPK14, MMP9 and PTGS2 crystal structure hydrogenation by Autodock software. (A) Songoramine and MAPK 14; (B) songoramine and PTGS 2; (C) songoramine and MMP9 (hydrogen bonds are indicated by dashed lines, distances in angstroms.)

Detailed Description

For the purpose of better explaining the present invention and to facilitate understanding, the present invention will be described in detail by way of specific embodiments with reference to the accompanying drawings.