阅读说明：本技术 一种青楷槭中多酚类成分的提取方法 (Method for extracting polyphenol components in acer tegmentosum ) 是由 张昌浩 尹雄杰 金莉莉 郑爱萍 于 2020-08-10 设计创作，主要内容包括：本发明公开了一种一种青楷槭中多酚类成分的提取方法,包括以下步骤：取青楷槭干燥茎皮,称重；以料液比1g：(20-40)mL的比例加入95%乙醇溶液,浸泡一周；加热回流提取,提取温度75℃-85℃,提取时间2h-4h,提取3次,过滤并合并滤液；合并滤液减压浓缩至浸膏,放入烘箱干燥；烘干后制得浸膏,称重,计算提取率。本发明采用溶剂提取法提取青楷槭中多酚类物质,具有工艺简便,提取率高,提取物活性强,很好的抗氧化能力等优点,具有较好的应用前景。(The invention discloses a method for extracting polyphenol components in acer tegmentosum, which comprises the following steps: taking dry bark of the acer tegmentosum, and weighing; 1g of liquid-material ratio: (20-40) mL, adding a 95% ethanol solution, and soaking for one week; heating, refluxing and extracting for 3 times at 75-85 deg.C for 2-4 h, filtering, and mixing filtrates; mixing filtrates, concentrating under reduced pressure to obtain extract, and drying in oven; drying to obtain extract, weighing, and calculating the extraction rate. The method for extracting the polyphenol substances in the acer tegmentosum by adopting the solvent extraction method has the advantages of simple and convenient process, high extraction rate, strong extract activity, good oxidation resistance and the like, and has better application prospect.)

1. A method for extracting polyphenol components in acer tegmentosum includes the following steps:

taking dry stem skin of Acer tataricus, and weighing;

the ratio of the materials to the liquid is 1g: (20-40) mL, adding a 95% ethanol solution, and soaking for one week;

performing heating reflux extraction for 3 times at the extraction temperature of 75-85 ℃ for 2-4 h, filtering and combining the filtrate;

fourthly, merging the filtrate, decompressing and concentrating the filtrate to obtain an extract, and drying the extract in an oven;

and fifthly, drying to obtain the extract.

2. The method for extracting the polyphenol components in the acer tegmentosum according to claim 1, wherein the method comprises the following steps: the proportion of the dry acer tegmentosum to 95% ethanol is 1g to 30 mL.

3. The method for extracting the polyphenol components in the acer tegmentosum according to claim 1, wherein the method comprises the following steps: the temperature of the reflux extraction is 80 ℃, and the time of each reflux extraction is 3 h.

Technical Field

The invention belongs to the technical field of plant extraction, and particularly relates to a method for extracting polyphenol components in acer tegmentosum.

Background

Acer rubrum (A. chinensis)Acer tegmentosumMaxim) is plant of Aceraceae, such as Acer niveum, Acer rubrum, or Acer apis. Mainly produced in Changbai mountain area of Jilin province in northeast China. The main bioactive component is polyphenol. The acer tegmentosum has pharmacological effects of resisting inflammation, resisting oxidation, protecting the liver, treating traumatic hemorrhage and the like, has no toxicity or side effect, and has wide application prospect in the aspects of medicines and health-care products.

Polyphenols, called "seventh group nutrients", are one of the natural active substances which are currently attracting much attention, and are also an important group of pharmacological and nutritional factors. Polyphenol has strong antioxidant effect, is called as natural antioxidant, and has unique effects of resisting cancer, resisting inflammation, resisting virus, reducing blood sugar, etc. The extraction process mainly comprises a solvent extraction method, an ultrasonic extraction method, a microwave extraction method, a biological enzyme degradation method, a resin adsorption extraction method and the like, but various extraction methods have advantages and disadvantages.

Disclosure of Invention

The invention aims to provide an extraction method of an optimal process for efficiently extracting polyphenol components from acer tegmentosum.

The technical scheme proposed for achieving the purpose is as follows:

a method for extracting polyphenol components in acer tegmentosum includes the following steps:

taking dry stem skin of Acer tataricus, and weighing;

the ratio of the materials to the liquid is 1g: (20-40) mL, adding a 95% ethanol solution, and soaking for one week;

performing heating reflux extraction for 3 times at the extraction temperature of 75-85 ℃ for 2-4 h, filtering and combining the filtrate;

fourthly, merging the filtrate, decompressing and concentrating the filtrate to obtain an extract, and drying the extract in an oven;

fifthly, drying to obtain an extract, weighing, and calculating the extraction rate.

Further, the proportion of the dry bark of the acer tegmentosum to 95% ethanol is 1g:30 mL.

Further, the temperature of the reflux extraction is 80 ℃, and the time of each reflux extraction is 3 hours.

The invention provides an extraction process of polyphenol components in acer tegmentosum, which adopts an ethanol solution to carry out heating reflux extraction, can obtain a higher extraction effect in a shorter time, and effectively improves the utilization rate of the acer tegmentosum.

The extraction process disclosed by the invention has the advantages of simple process, high extraction speed, high extraction rate of polyphenol components, strong activity and the like, and has a better extraction effect. The extract has good antioxidant activity, and can be used for preparing liver protection medicine or health product.

Drawings

FIG. 1 is a line graph showing the antioxidant ability of Acer tegmentosum (Acer. tegmentosum) Roxb. polyphenol extract and Vc in an example test.

Detailed Description

A method for extracting polyphenol components in acer tegmentosum includes the following steps:

taking dry stem skin of Acer tataricus, and weighing;

the ratio of the materials to the liquid is 1g: (20-40) mL, adding a 95% ethanol solution, and soaking for one week;

performing heating reflux extraction for 3 times at the extraction temperature of 75-85 ℃ for 2-4 h, filtering and combining the filtrate;

fourthly, merging the filtrate, decompressing and concentrating the filtrate to obtain an extract, and drying the extract in an oven;

fifthly, drying to obtain an extract, weighing, and calculating the extraction rate.

The extraction process of polyphenol components in the acer tegmentosum relates to 3 key conditions: material-liquid ratio, reflux extraction temperature and reflux extraction time. The following single factor experiment was performed for three aspects.

Influence of the feed-to-liquid ratio

Table 1 shows the results of a single-factor experiment of the feed-liquid ratio at an extraction temperature of 80 ℃ and an extraction time of 3h, wherein the results show that: the ratio of the feed to the liquid is 1g to 30 mL.

TABLE 1 Effect of feed liquid ratio on polyphenol extraction Rate

。

Influence of extraction temperature

Table 2 shows the single-factor experiment of the extraction temperature when the feed-liquid ratio is 1g:30mL and the extraction time is 3h

The results show that: the extraction temperature is preferably 80 deg.C.

TABLE 2 Effect of extraction temperature on Polyphenol extraction yield

。

Influence of extraction time

Table 3 shows the results of a single-factor experiment of extraction time at an extraction temperature of 80 ℃ with a feed-to-liquid ratio of 1g to 30 mL: the extraction time is preferably 3 h.

TABLE 3 Effect of extraction time on Polyphenol extraction yield

。

Orthogonal test of extraction process conditions of polyphenol components in acer tegmentosum

TABLE 4 results of orthogonal experiments

。

And (4) conclusion: through single factor experiment and orthogonal experiment, the extraction rate of polyphenol components under different extraction process conditions is obtained, and the optimal process conditions are that the material-liquid ratio is 1g:30mL, the extraction temperature is 80 ℃, and the extraction time is 3 hours.

Acer tegmentosum polyphenol extract reduction test

The reduction capability is an important expression of potential antioxidant activity of the plant extract, and the strength of the reduction capability shows the strength of the antioxidant activity of the plant extract to a certain extent. The antioxidant (reducing agent) is a compound which can scavenge free radicals by giving electrons through its own reducing action, and the stronger the reducing ability is, the stronger the oxidation resistance is. The reduction capability of the antioxidant substance is utilized to reduce the ferricyanate into ferricyanate, and the ferricyanate and ferric iron generate ferric ferrocyanide under an acidic medium. Therefore, the more ferrocyanide is produced, the greater the absorbance is, and the stronger the reducing ability is.

Vitamin c (Vc) is used as a positive control, and the antioxidant capacity of the Acer tegmentosum polyphenol extract is compared with that of Vc. From fig. 1, it can be seen that the acer tegmentosum phenolic extract and Vc both have certain antioxidant capacity, and the antioxidant activity is enhanced with the increase of concentration. When the mass concentration of the sample is more than 0.4 mg/mL, the antioxidant capacity of the Acer tegmentosum polyphenol extract is more than Vc, and the difference of the antioxidant activity increases along with the increase of the concentration.

The foregoing is only a preferred embodiment of the present invention and the present invention is not limited to the above examples. Modifications, changes or substitutions by one of ordinary skill in the art within the spirit of the present invention should be included within the scope of the present invention.