阅读说明：本技术 一种复合酶辅助提取橄榄多酚的方法 (Method for extracting olive polyphenol with assistance of complex enzyme ) 是由 陈徉 王姣 邹平 赵海坤 程泽龙 周国玲 闵琪 刘春慧 周倩 秦静 于 2019-03-28 设计创作，主要内容包括：本发明属于生物活性成分的提取领域,特别涉及一种复合酶辅助提取橄榄多酚的方法。本发明中使用纤维素酶、木质素酶和果胶酶,溶剂为50~70%的乙醇水溶液,辅助气体为氮气,氮气流速为0.6~1.0 L/min,橄榄果渣与乙醇水溶液的料液比为1:6~1:10,橄榄果渣纤维素酶、木质素酶和果胶酶的质量比为100:0.5:0.5:0.3,反应温度为45~60℃,pH为6.0~7.5,时间30~60 min。发明提供的提取方法提取效率高,多酚类物质的氧化损失少,可以在生产中推广使用。(The invention belongs to the field of extraction of bioactive components, and particularly relates to a method for extracting olive polyphenol with the assistance of complex enzyme. According to the method, cellulase, ligninase and pectinase are used, a solvent is 50-70% of an ethanol water solution, an auxiliary gas is nitrogen, the nitrogen flow rate is 0.6-1.0L/min, the material-liquid ratio of olive pomace to the ethanol water solution is 1: 6-1: 10, the mass ratio of the olive pomace cellulase to the ligninase to the pectinase is 100:0.5:0.5:0.3, the reaction temperature is 45-60 ℃, the pH value is 6.0-7.5, and the time is 30-60 min. The extraction method provided by the invention has high extraction efficiency and less oxidation loss of polyphenols, and can be popularized and used in production.)

1. A method for extracting olive polyphenol with the assistance of complex enzyme is characterized in that: the method comprises the following steps: proportionally adding olive pomace and an ethanol aqueous solution into an extraction device, setting a proper temperature, adjusting the pH value, waiting for the temperature to reach the required temperature, proportionally adding cellulase, ligninase and pectinase, and introducing nitrogen to start reaction.

2. The method for extracting olive polyphenol with the assistance of complex enzyme as claimed in claim 1, which is characterized in that: the auxiliary gas is nitrogen, and the flow rate of the nitrogen is 0.6-1.0L/min.

3. The method for extracting olive polyphenol with the assistance of complex enzyme as claimed in claim 1, which is characterized in that: the feed-liquid ratio of the olive pomace to the ethanol water solution is 1: 6-1: 10.

4. The method for extracting olive polyphenol with the assistance of complex enzyme as claimed in claim 1, which is characterized in that: the mass ratio of the olive pomace cellulase to the ligninase to the pectinase is 100:0.5:0.5: 0.3.

5. The method for extracting olive polyphenol with the assistance of complex enzyme as claimed in claim 1, which is characterized in that: the reaction temperature is 45-60 ℃, the pH is 6.0-7.5, and the reaction time is 30-60 min.

Technical Field

The invention belongs to the field of extraction of bioactive components, and particularly relates to a method for extracting olive polyphenol with the assistance of complex enzyme.

Background

The polyphenol substances are compounds with the functions of resisting oxidation, resisting aging, preventing cancer and the like in natural plants such as tea leaves and the like. Has various physiological activities of resisting radiation, preventing cardiovascular diseases, reducing blood fat, blood sugar and the like, and is a natural food additive which is generally recognized as safe and efficient. However, the olive oil and related products thereof are popular worldwide, and the research on the method for efficiently extracting the olive polyphenol has important significance.

The polyphenol substances are usually extracted by an extraction tank provided with a mechanical stirring device, so that the oxidation loss is inevitable, the complex enzyme auxiliary extraction is a high-efficiency extraction method, and the nitrogen assists mass transfer and isolates air, so that the problems can be effectively solved.

Disclosure of Invention

The invention provides a method for extracting olive polyphenol with the assistance of complex enzyme, which comprises the following steps:

proportionally adding olive pomace and an ethanol aqueous solution into an extraction device, setting a proper temperature, adjusting the pH value, waiting for the temperature to reach the required temperature, proportionally adding cellulase, ligninase and pectinase, and introducing nitrogen to start reaction. The method comprises the following steps of preparing cellulase, ligninase and pectinase, wherein a solvent is 50-70% of an ethanol aqueous solution, an auxiliary gas is nitrogen, the nitrogen flow rate is 0.6-1.0L/min, the material-liquid ratio of olive pomace to the ethanol aqueous solution is 1: 6-1: 10, the mass ratio of the olive pomace cellulase, ligninase and pectinase is 100:0.5:0.5:0.3, the reaction temperature is 45-60 ℃, the pH value is 6.0-7.5, and the time is 30-60 min. The extraction method provided by the invention has high extraction efficiency and less oxidation loss of polyphenols, and can be popularized and used in production.

Drawings

Figure 1 influence of nitrogen assistance on enzymatic extraction.

Detailed Description

The invention will now be further described with reference to specific examples and one of the examples will be selected to provide data support for this patent, which examples are intended to illustrate the invention and not to further limit the invention.