阅读说明：本技术 一种超声波逆流提取***二酚的方法 (Ultrasonic countercurrent extraction method of cannabidiol ) 是由 姚德坤 万莉 姚德利 于 2020-06-25 设计创作，主要内容包括：本发明属于化工技术领域,涉及一种富集纯化大麻二酚的方法,特别是涉及一种利用超声波逆流提取、大孔吸附树脂吸附、柱层析纯化大麻中大麻二酚的方法。本发明优点：1、本发明采用超声波提取,以无水乙醇、甲醇为溶剂,除掉部分脂肪油,大大降低了提取时间,提高了生产效率,方法简单。2、大孔树脂再生方便迅速,适合大生产,且成本大大降低,容易操作。3、大孔树脂和硅胶柱层析结合,分离和纯化效果好,大大提高了产品纯度。本发明采用了超声波逆流提取、大孔树脂分离纯化和柱层析进一步纯化,产品纯度大大提高,最终得到含量65％以上的大麻二酚和含量90％以上的大麻二酚终产品。(The invention belongs to the technical field of chemical industry, relates to a method for enriching and purifying cannabidiol, and particularly relates to a method for purifying cannabidiol in cannabis sativa by utilizing ultrasonic countercurrent extraction, macroporous adsorption resin adsorption and column chromatography. The invention has the advantages that: 1. the invention adopts ultrasonic extraction, takes absolute ethyl alcohol and methanol as solvents to remove partial fatty oil, greatly reduces extraction time, improves production efficiency and has simple method. 2. The macroporous resin is convenient and rapid to regenerate, is suitable for large-scale production, greatly reduces the cost and is easy to operate. 3. The macroporous resin and the silica gel column are combined for chromatography, the separation and purification effects are good, and the product purity is greatly improved. The invention adopts ultrasonic countercurrent extraction, macroporous resin separation and purification and column chromatography for further purification, greatly improves the product purity, and finally obtains the cannabidiol with the content of more than 65 percent and the cannabidiol with the content of more than 90 percent.)

1. A method for purifying cannabidiol in hemp by ultrasonic countercurrent extraction, macroporous adsorption resin adsorption and column chromatography comprises the following steps:

(1) ultrasonic countercurrent extraction:

drying industrial hemp leaves, stems or roots, crushing the dried industrial hemp leaves, stems or roots to 40-60 meshes, adding absolute ethyl alcohol and methanol as solvents, performing ultrasonic countercurrent extraction under the conditions of 400-600W, 10-30 min and a material-liquid ratio of 1: 4-1: 8, feeding in the forward direction, feeding water in the reverse direction, wherein the material and water flow is in a reverse continuous dynamic flow. Adding petroleum ether into the extracting solution for layering, and removing fatty oil;

(2) macroporous resin adsorption:

recovering ethanol until no ethanol exists, adsorbing the obtained solution with macroporous resin of one of DA201, D101, HPD300 and AB-8 resin, wherein the height-diameter ratio of the resin column is 8: 1-15: 1;

(3) ethanol elution:

eluting with 40-95% ethanol solution at flow rate of 1-3BV/h and elution volume of 6-8BV, and monitoring the eluate by thin layer chromatography;

(4) concentration:

putting the adsorption solution into a single-effect concentration tank, concentrating the adsorption solution at 70-80 ℃ under the vacuum degree of 0.075Mpa until the volume of the extraction solution is 1: 20-1: 22, and recovering ethanol; collecting ethanol eluent, concentrating again under vacuum condition, wherein the concentration temperature is 180-230 ℃, the evaporation time is 40-60 min, and recovering ethanol;

(5) obtaining a cannabidiol crude extract:

drying the obtained concentrated solution under reduced pressure to obtain coarse powder of cannabidiol semi-finished product with content of more than 65%;

(6) silica gel column chromatography:

dissolving the obtained cannabidiol coarse powder in ethyl acetate, adding a proper amount of silica gel, stirring, drying, sieving with a 60-80-mesh sieve, loading by a dry method, performing 200-mesh and 300-mesh silica gel column chromatography on an industrial-grade column, eluting by using chloroform-methanol or petroleum ether-ethyl acetate solution in a ratio of 95-85: 2, performing tracking detection by using a thin layer chromatography, and collecting the eluent of each stage of cannabidiol;

(7) and (3) drying:

concentrating the collected cannabidiol eluent, spraying the concentrated cannabidiol eluent and drying the powder to obtain powder with the content of more than 90 percent.

2. The method for purifying cannabidiol from cannabis sativa by ultrasonic countercurrent extraction, macroporous adsorbent resin adsorption and column chromatography as claimed in claim 1, wherein: in the step (1), the hemp leaves, stems or roots are dried, crushed and sieved by a 40-60-mesh sieve, and the solvent is absolute ethyl alcohol or methanol solution.

3. The method for purifying cannabidiol in cannabis sativa by ultrasonic countercurrent extraction, macroporous adsorbent resin adsorption and column chromatography as claimed in claim 2, wherein: in the step (1), the ultrasonic treatment power is 400-600W, the time is 10-30 min, and the material-liquid ratio is 1: 4-1: 8.

4. The method for purifying cannabidiol from cannabis sativa by ultrasonic countercurrent extraction, macroporous adsorbent resin adsorption and column chromatography as claimed in claim 3, wherein: in the step (2), the type of the macroporous resin is one of DA201, D101, HPD300 and AB-8 resin, and the height-diameter ratio of the resin column is 8: 1-15: 1.

5. The method for purifying cannabidiol from cannabis sativa by ultrasonic countercurrent extraction, macroporous adsorbent resin adsorption and column chromatography as claimed in claim 4, wherein: in the step (3), the flow rate of ethanol elution is 1-3BV/h, the volume of elution is 6-8BV, and the concentration of ethanol solution is 40% -95%.

6. The method for purifying cannabidiol from cannabis sativa by ultrasonic countercurrent extraction, macroporous adsorbent resin adsorption and column chromatography as claimed in claim 5, wherein: in the step (4), the volume of the concentrated extracting solution is 1: 20-1: 22 of the original volume.

7. The method for purifying cannabidiol from cannabis sativa by ultrasonic countercurrent extraction, macroporous adsorbent resin adsorption and column chromatography as claimed in claim 6, wherein: in the step (6), before the silica gel column chromatography, the cannabidiol coarse powder is dissolved in ethyl acetate, dried and sieved by a 60-80 mesh sieve, and is sieved by an industrial-grade column of 200-300 mesh.

8. The method for purifying cannabidiol from cannabis sativa by ultrasonic countercurrent extraction, macroporous adsorbent resin adsorption and column chromatography as claimed in claim 7, wherein: in the step (6), an elution solvent is one of chloroform-methanol or petroleum ether-ethyl acetate solution, the elution ratio is 95-85: 2, and tracking detection is performed by thin layer chromatography.

The technical field is as follows:

the invention belongs to the technical field of chemical industry, relates to a method for ultrasonic countercurrent extraction of cannabidiol, and particularly relates to a method for purifying cannabidiol by ultrasonic countercurrent extraction, resin adsorption and column chromatography.

Background art:

industrial Cannabis is known as hemp (hemp) in China and is a perennial herb of the Cannabis genus (Cannabinaceae) in the Cannabinaceae family. The industrial hemp refers to the hemp with the tetrahydrocannabinol content of less than 0.3 percent, the legal industrial hemp is a variety with low toxicity, the industrial hemp (THC is less than 0.3 percent) is not considered to have the drug utilization value, but still is a treasure on the whole body, the application at least comprises the fields of textile, paper making, food, medicine, sanitation, daily chemicals, leather, automobile, building, decoration, packaging and the like, and the industrial hemp is a classical production raw material.

Cannabidiol (CBD) is an important non-psychoactive substance in hemp, can antagonize hallucinogenic effect of THC, has multiple physiological activities such as antioxidation, antitumor, anti-inflammatory, anxiolytic, antispasmodic, and anti-drug-resistant epilepsy, and is approved by FDA for clinical treatment of Lennox-Gastaut syndrome and Dravet syndrome.

The China-hemp contains numerous phenolic compounds with similar properties to CBD, and a scientific and effective extraction and separation mode is a prerequisite for obtaining high-purity CBD and also a necessary condition for ensuring the popularization and the application of the China-hemp. At present, cannabidiol is mainly extracted by a traditional organic solvent method, a CO2 supercritical liquid extraction method, a eutectic solvent extraction method, an ultrasonic-assisted extraction method, an enzymatic extraction method and the like, and is mainly purified by solvent precipitation, column chromatography, molecular distillation, crystallization and the like. However, a single extraction and separation mode is often difficult to ensure that no other compounds such as THC with similar structures and harmful to human bodies remain in the CBD, so that it is important to invent a method for producing CBD in a large scale with low cost and high efficiency by combining a plurality of extraction and purification modes.

The invention adopts ultrasonic countercurrent extraction equipment, shortens the extraction time, adopts macroporous resin separation and purification and column chromatography for further purification which are suitable for large-scale production operation, and greatly improves the product purity.

The invention content is as follows:

the invention adopts the ultrasonic countercurrent extraction, macroporous resin adsorption and column chromatography purification technologies, greatly reduces the extraction time, improves the production efficiency and has simple method. The macroporous resin is convenient and rapid to regenerate, is suitable for large-scale production, greatly reduces the cost, has good separation and purification effects, and adopts recrystallization in the later period, so that the product obtained by the invention has high purity, obviously improves the content of effective components, and overcomes the defects of low purity, long time and the like of the conventional extraction method.

In order to achieve the purpose, the invention adopts the technical scheme that:

a method for purifying cannabidiol in hemp by ultrasonic countercurrent extraction, macroporous adsorption resin wax adsorption and column chromatography comprises the following steps:

(1) ultrasonic countercurrent extraction:

drying industrial hemp leaves, stems or roots, crushing the dried industrial hemp leaves, stems or roots to 40-60 meshes, adding absolute ethyl alcohol and methanol as solvents, performing ultrasonic countercurrent extraction under the conditions of 400-600W, 10-30 min and a material-liquid ratio of 1: 4-1: 8, feeding in the forward direction, feeding water in the reverse direction, wherein the material and water flow is in a reverse continuous dynamic flow. Adding petroleum ether into the extracting solution for layering, and removing fatty oil;

(2) macroporous resin adsorption:

recovering ethanol until no ethanol exists, adsorbing the obtained solution with macroporous resin of DA201, D101, HPD300, and AB-8 resin column with height-diameter ratio of 8: 1-15: 1;

(3) eluting with ethanol;

eluting with 40-95% ethanol solution at flow rate of 1-3BV/h and elution volume of 6-8BV, and monitoring the eluate by thin layer chromatography;

(4) concentration:

putting the adsorption solution into a single-effect concentration tank, concentrating the adsorption solution at 70-80 ℃ under the vacuum degree of 0.075Mpa until the volume of the extraction solution is 1: 20-1: 22, and recovering ethanol; collecting ethanol eluent, concentrating again under vacuum condition, wherein the concentration temperature is 180-230 ℃, the evaporation time is 40-60 min, and recovering ethanol;

(5) obtaining a cannabidiol crude extract:

drying the obtained concentrated solution under reduced pressure to obtain coarse powder of cannabidiol semi-finished product with content of more than 65%;

(6) silica gel column chromatography:

dissolving the obtained cannabidiol coarse powder in ethyl acetate, adding a proper amount of silica gel, stirring, drying, sieving with a 60-80-mesh sieve, loading by a dry method, performing 200-mesh and 300-mesh silica gel column chromatography on an industrial-grade column, eluting by using chloroform-methanol or petroleum ether-ethyl acetate solution in a ratio of 95-85: 2, performing tracking detection by using a thin layer chromatography, and collecting the eluent of each stage of cannabidiol;

(7) and (3) drying:

concentrating the collected cannabidiol eluent, spraying the concentrated cannabidiol eluent and drying the powder to obtain powder with the content of more than 90 percent.

The specific implementation mode is as follows: