阅读说明：本技术 一种乳酸链球菌素的提取方法 (Extraction method of nisin ) 是由 王婷 程豪 宋志倩 王绘砖 徐珍 袁昉 于 2020-05-28 设计创作，主要内容包括：本发明属于食品防腐剂技术领域,具体涉及一种乳酸链球菌素的提取方法,利用乳酸链球菌素自身菌体吸附的特性,将乳酸链球菌素发酵液用陶瓷膜进行浓缩,取菌体用酸进行酸解、过滤得含有乳酸链球菌素的滤液。滤液经过膜过滤进一步纯化,加盐喷干可得乳酸链球菌素成品。本发明耗能低、不使用有机溶剂且不产生含高盐废水。(The invention belongs to the technical field of food preservatives, and particularly relates to a method for extracting nisin. The filtrate is further purified by membrane filtration, and the finished product of nisin can be obtained by adding salt and spraying the salt to dry. The invention has low energy consumption, does not use organic solvent and does not generate high-salt wastewater.)

1. The extraction method of nisin is characterized by comprising the following steps:

a, filtering fermentation liquor: and directly filtering the nisin fermentation liquor obtained by fermentation to obtain concentrated bacterial liquid.

b, acid hydrolysis: and carrying out acidolysis on the concentrated bacterial liquid by using acid, and heating the acidolyzed bacterial liquid.

c, filtering bacterial liquid: and further filtering the acidolyzed bacteria liquid by using a ceramic membrane to obtain filtrate.

d, membrane filtration: and c, filtering the filtrate obtained in the step c for 2 times by using a membrane to remove impurities to obtain a concentrated solution.

e, adding salt: and d, calculating the titer of the concentrated solution in the step d, adding sodium chloride, and then spraying to dry to obtain a finished product.

2. The method for extracting nisin according to claim 1, wherein the filtration in step a is one of ceramic membrane filtration, plate and frame filter press filtration and centrifuge filtration.

3. The extraction method of nisin according to claim 1, comprising the following steps: the acid in the step b is an inorganic acid.

4. The method for extracting nisin according to claim 1, wherein the filtration in step c is one of ceramic membrane filtration, plate and frame filter press filtration and centrifuge filtration.

Technical Field

The invention belongs to the technical field of food preservatives, and particularly relates to a method for extracting nisin.

Background

The Nisin is a safe, nontoxic and efficient natural food preservative. Can be used for preserving meat products, dairy products, vegetable protein food, canned food, etc.

The extraction method of Nisin reported at present comprises the following steps:

1. patent CN200710072250.6 discloses that the bottom of fermentation liquor is bubbled, the foam is collected, the foam is filtered and concentrated, then salting out is carried out, the salting-out solution is filtered and separated, and the solid phase is re-dissolved, added with salt and then sprayed to dry to obtain the finished product of nisin. The method has long extraction period, and generates a large amount of wastewater containing high-concentration sodium chloride in the salting-out process, thereby causing great burden to sewage treatment.

2 patent CN201110230940.6 mentions acidifying the fermentation broth, precipitating nisin with dichloromethane and drying the solid in vacuum. In the method, an organic solvent dichloromethane is adopted, so that solvent residues are easily caused.

3 the main method for extracting Nisin at present is to acidify and heat the fermentation liquor, then filter the fermentation liquor by using a ceramic membrane, evaporate and concentrate the filtrate, and then salt out. The method has the disadvantages that the evaporation concentration consumes more energy, and a large amount of waste water containing high-concentration sodium chloride is generated in the salting-out process, thereby causing great burden to sewage treatment.

Disclosure of Invention

In order to solve the problems in the prior art, the invention provides a method for producing nisin, which has low energy consumption, does not use organic solvent and does not generate high-salt wastewater.

The invention also provides a preparation method of the traditional Chinese medicine ointment for treating the closed injury of the pets, which has the advantages of standard steps, simplicity and understandability and is suitable for mass production.

The invention adopts the specific technical scheme that: a method for extracting nisin comprises the following steps:

a, filtering fermentation liquor: and directly filtering the nisin fermentation liquor obtained by fermentation to obtain concentrated bacterial liquid.

b, acid hydrolysis: and carrying out acidolysis on the concentrated bacterial liquid by using acid, and heating the acidolyzed bacterial liquid.

c, filtering bacterial liquid: and further filtering the acidolyzed bacteria liquid by using a ceramic membrane to obtain filtrate.

d, membrane filtration: and c, filtering the filtrate obtained in the step c for 2 times by using a membrane to remove impurities to obtain a concentrated solution.

e, adding salt: and d, calculating the titer of the concentrated solution in the step d, adding sodium chloride, and then spraying to dry to obtain a finished product.

And the filtering mode in the step a is one of ceramic membrane filtering, plate-and-frame filter press filtering and centrifuge filtering.

The acid in the step b is an inorganic acid.

And c, filtering in the step c by using one of ceramic membrane filtering, plate-and-frame filter press filtering and centrifuge filtering.

The design idea of the invention is as follows: by utilizing the characteristic of the adsorption of the nisin by the bacteria, the nisin fermentation liquor is concentrated by a ceramic membrane, and the bacteria are subjected to acidolysis and filtration by acid to obtain a filtrate containing nisin. The filtrate is further purified by membrane filtration, and the finished product of nisin can be obtained by adding salt and spraying the salt to dry. The invention has low energy consumption, does not use organic solvent and does not generate high-salt wastewater.

Detailed Description

The invention will be further illustrated with reference to specific examples: