阅读说明：本技术 一种乳化型水基金属加工液的生物抑菌剂及其制备方法 (Biological bacteriostatic agent of emulsified water-based metal working fluid and preparation method thereof ) 是由 赵朋飞 李小磊 戴媛静 张晨辉 雒建斌 于 2020-07-17 设计创作，主要内容包括：本发明提供了一种乳化型水基金属加工液的生物抑菌剂及其制备方法,包括微生物菌体和助剂；所述微生物菌体为双歧杆菌、短乳杆菌、绿脓杆菌、变形杆菌、丁酸梭菌中的一种或几种；所述助剂包括组分A、组分B和组分C,所述组分A为乳酸菌肽、吩嗪、变形菌素、碱性蛋白酶、弹性蛋白酶中的一种或几种,所述组分B为天冬氨酸、精氨酸、缬氨酸、脯氨酸、赖氨酸、亮氨酸中的一种或几种,所述组分C为聚乙烯吡咯烷酮、肌醇、抗坏血酸、鼠李糖脂、戊二酸中的一种或几种。本发明所述的生物抑菌剂不仅可以有效延长金属加工液的使用周期,还可以减少化学杀菌剂对环境和人体的危害。(The invention provides a biological bacteriostatic agent of emulsified water-based metal working fluid and a preparation method thereof, comprising microbial thalli and an auxiliary agent; the microbial thallus is one or more of bifidobacterium, lactobacillus brevis, pseudomonas aeruginosa, bacillus proteus and clostridium butyricum; the auxiliary agent comprises a component A, a component B and a component C, wherein the component A is one or more of lactobacillus peptide, phenazine, proteobacteria, alkaline protease and elastase, the component B is one or more of aspartic acid, arginine, valine, proline, lysine and leucine, and the component C is one or more of polyvinylpyrrolidone, inositol, ascorbic acid, rhamnolipid and glutaric acid. The biological bacteriostatic agent provided by the invention can effectively prolong the service life of the metal working fluid and reduce the harm of a chemical bactericide to the environment and human body.)

1. A biological bacteriostatic agent of emulsified water-based metal working fluid is characterized in that: comprises microbial thallus and auxiliary agent; the microbial thallus is a dominant growth microbe, and the dominant growth microbe is one or more of bifidobacterium, lactobacillus brevis, pseudomonas aeruginosa, proteus and clostridium butyricum; the auxiliary agent comprises a component A, a component B and a component C, wherein the component A is one or more of lactobacillus peptide, phenazine, proteobacteria, alkaline protease and elastase, the component B is one or more of aspartic acid, arginine, valine, proline, lysine and leucine, and the component C is one or more of polyvinylpyrrolidone, inositol, ascorbic acid, rhamnolipid and glutaric acid.

2. The biological bacteriostatic agent for emulsified water-based metalworking fluid according to claim 1, wherein: the biological bacteriostatic agent comprises the following components in percentage by mass: 1 to 5 percent of microbial thallus and 0.04 to 0.09 percent of component A; 0.5 to 0.9 percent of component B; 0.6 to 0.9 percent of component C; the rest is sterilized deionized water.

3. The biological bacteriostatic agent for emulsified water-based metalworking fluid according to claim 1, wherein: the number of the living bacteria in the biological bacteriostatic agent is at least 30 hundred million/mL.

4. The biological bacteriostatic agent for emulsified water-based metalworking fluid according to claim 1, wherein: the microbial thalli are extracted from a natural water body and are obtained through intercropping and propagation.

5. A method of preparing a biological bacteriostatic agent for an emulsified water-based metalworking fluid according to any one of claims 1 to 4, wherein the biological bacteriostatic agent comprises: the method comprises the following steps:

(1) under the aseptic operation condition, selecting a slant for preserving strains, streaking a three-region strain onto an LB (Langmuir Blodgett) plate culture medium, and performing activated culture at 36 ℃ for 24 hours to obtain a single colony;

(2) picking single colony from an LB flat plate solid culture medium, transferring the single colony into an LB liquid culture medium, and culturing for 48 hours in a constant temperature oscillator at 36 ℃ and 120r/min to obtain a bacterial liquid;

(3) repeatedly washing and centrifuging the bacterial liquid by using sterile normal saline, washing for 3-4 times, and removing supernatant to obtain precipitate, namely microbial thalli;

(4) and mixing the microbial thallus with the auxiliary agent, and then adding the mixture into sterilized deionized water to obtain the biological bacteriostatic agent.

6. An emulsified water-based metalworking fluid of a biological bacteriostatic agent according to any one of claims 1 to 5 wherein: the emulsified water-based metal working fluid contains 0.5-3.5% of biological bacteriostatic agent by mass percent.

Technical Field

The invention belongs to the field of metal working fluids, and particularly relates to a biological bacteriostatic agent for an emulsified water-based metal working fluid and a preparation method thereof.

Background

The metal working fluid is a fluid that plays a role in cooling, lubrication, rust prevention, cleaning, and the like during metal working, and is generally composed of a base oil such as most of mineral oil, vegetable oil, synthetic oil, and the like, and a small amount of various additives. The emulsified metal working fluid has a key problem that the O/W type emulsified working fluid is easy to infect microorganisms and decay, because the base oil and certain additives in the metal working fluid are nutrient substances for the survival and propagation of the microorganisms. When a large amount of microorganisms are propagated, the metal working fluid is reduced in pH value, reduced in lubricating performance, rust prevention performance and the like, shortened in service life, and generates an unpleasant odor, worsens the working environment and harms the physical health of operators. The common solution at present is to add chemical bactericides to inhibit the growth of putrefying bacteria. The main types of chemical bactericides are formaldehyde, alkane derivatives, morpholines, phenols, quaternary ammonium salt compounds and the like. However, the addition of chemical germicides inevitably causes harm to human or environment, and the long-term use of chemical germicides results in the resistance of microorganisms to lose the effect.

Patent application with publication number CN109797025A provides a biological-method-based water-based cutting fluid deodorant, which adopts alcohol chemical reagents to perform early sterilization and bacteriostasis, and then adds yeast dry powder or yeast viable bacteria liquid to perform bacteriostasis. The examples show that the chemical and biological deodorization method is suitable for deodorization treatment of a waste cutting fluid or an in-use cutting fluid, but the invention does not describe whether the addition of the deodorization agent affects the usability (such as rust prevention, lubrication, and the like) and the system stability (particularly, metastable emulsion type cutting fluid), and the yeast agent is also effective against putrefying bacteria which are liable to cause odor, such as anaerobic bacteria and facultative anaerobic bacteria.

Therefore, the research and development of a biological bacteriostatic agent which can effectively inhibit the mass propagation of microorganisms in the using process of the processing liquid and can ensure that the using performance and the system stability of the processing liquid are not influenced is a technical problem to be solved in the field.

Disclosure of Invention

In view of the above, the present invention provides a biological bacteriostatic agent for an emulsified water-based metal working fluid and a preparation method thereof, which solve the problem that the emulsified water-based metal working fluid is easy to decay, and provides a biological bacteriostatic agent suitable for an emulsified water-based metal working fluid, wherein the microbial bacteriostatic agent can be directly added into the metal working fluid, so as to prolong the service life of the metal working fluid on the basis of not affecting the performances of rust prevention, lubrication and the like of the metal working fluid. The use of the microbial bacteriostatic agent can reduce or even avoid the use of chemical bactericides, and is an environment-friendly bacteriostatic agent.

In order to achieve the purpose, the technical scheme of the invention is realized as follows:

a biological bacteriostatic agent of emulsified water-based metal working fluid comprises microbial thallus and auxiliary agent; the microbial thallus is a dominant growth microbe, and the dominant growth microbe is one or more of bifidobacterium, lactobacillus brevis, pseudomonas aeruginosa, proteus and clostridium butyricum; the auxiliary agent comprises a component A, a component B and a component C, wherein the component A is one or more of lactobacillus peptide, phenazine, proteobacteria, alkaline protease and elastase, the component B is one or more of aspartic acid, arginine, valine, proline, lysine and leucine, and the component C is one or more of polyvinylpyrrolidone, inositol, ascorbic acid, rhamnolipid and glutaric acid.

Preferably, the biological bacteriostatic agent comprises the following components in percentage by mass: 1 to 5 percent of microbial thallus and 0.04 to 0.09 percent of component A; 0.5 to 0.9 percent of component B; 0.6 to 0.9 percent of component C; the rest is sterilized deionized water.

Preferably, the number of viable bacteria in the biological bacteriostatic agent is at least 30 hundred million/mL.

Preferably, the microbial thalli are extracted from a natural water body and obtained through interspecies propagation.

The invention also provides a preparation method of the biological bacteriostatic agent for the emulsified water-based metal working fluid, which comprises the following steps:

(1) under the aseptic operation condition, selecting a slant for preserving strains, streaking a three-region strain onto an LB (Langmuir Blodgett) plate culture medium, and performing activated culture at 36 ℃ for 24 hours to obtain a single colony;

(2) picking single colony from an LB flat plate solid culture medium, transferring the single colony into an LB liquid culture medium, and culturing for 48 hours in a constant temperature oscillator at 36 ℃ and 120r/min to obtain a bacterial liquid;

(3) repeatedly washing and centrifuging the bacterial liquid by using sterile normal saline, washing for 3-4 times, and removing supernatant to obtain precipitate, namely microbial thalli;

(4) and mixing the microbial thallus with the auxiliary agent, and then adding the mixture into sterilized deionized water to obtain the biological bacteriostatic agent.

The invention also provides an emulsified water-based metal processing liquid applying the biological bacteriostatic agent, wherein the emulsified water-based metal processing liquid contains the biological bacteriostatic agent with the mass percentage of 0.5-3.5%.

According to the invention, the 'biological bacteriostasis' technology of adding microorganisms to inhibit the mass propagation of uncontrollable mixed bacteria is realized on the basis of not influencing the pH value, the antirust performance and the lubricating performance of the processing liquid by adding the microbial bacteriostat into the metal processing liquid. The biological bacteriostatic agent can effectively prolong the service life of the metal working fluid, can reduce the harm of chemical germicides to the environment and human body, and has very important significance for the development of a new generation of long-life environment-friendly water-based metal working fluid.

Compared with the prior art, the biological bacteriostatic agent of the emulsified water-based metal working fluid and the preparation method thereof have the following advantages:

(1) balancing water body microorganisms: the invention adopts a biological control technology of 'biological bacteriostasis', utilizes dominant growth microorganisms to inhibit the propagation of mixed bacteria in the metal working fluid, prevents the metal working fluid from being corrupted and prolongs the service life of the metal working fluid on the basis of not influencing the performances of rust prevention, lubrication and the like of the metal working fluid.

(2) Safety and environmental protection: the use of the biological bacteriostatic agent can reduce or even avoid the use of chemical bactericides and reduce the harm to the environment and operators; and secondly, the microbial bacteriostatic agent also has the advantages of low cost, safe use, high economic benefit, no environmental pollution and the like.

Drawings

FIG. 1 shows the colony morphology of Y1 (Pseudomonas aeruginosa) in example 1;

FIG. 2 shows the bacteriostatic effect of 0% bacteriostatic agent + 0% bacteriocide in example 1 (day 56);

FIG. 3 shows the bacteriostatic effect of 1.5% bacteriostatic agent + 0% bacteriocide in example 1 (day 56);

FIG. 4 shows the bacteriostatic effect of 1.5% bacteriostatic agent + 0.05% bacteriocide in example 1 (day 56);

FIG. 5 shows the bacteriostatic effect of 1.5% bacteriostatic agent + 0.075% bacteriocide in example 1 (day 56);

FIG. 6 is a graph showing the effect of bacteriostatic agent on pH at different times in example 1;

fig. 7 is a graph of the effect of bacteriostatic on lubricity in example 1 (day 56), with initial addition of: a.0% bacteriostatic agent + 0% bactericide, B.1.5% bacteriostatic agent + 0% bactericide, C.1.5% bacteriostatic agent + 0.05% bactericide, D.1.5% bacteriostatic agent + 0.075% bactericide, E.0% bacteriostatic agent + 0.15% bactericide.

Detailed Description

Unless defined otherwise, technical terms used in the following examples have the same meanings as commonly understood by one of ordinary skill in the art to which the present invention belongs. The test reagents used in the following examples, unless otherwise specified, are all conventional biochemical reagents; the experimental methods are conventional methods unless otherwise specified.

The present invention will be described in detail with reference to the following examples and accompanying drawings.