阅读说明：本技术 水稻组蛋白去甲基化酶jmj708在水稻育种中的应用 (Application of rice histone demethylase JMJ708 in rice breeding ) 是由 魏金环 陈忠科 安镇兴 于 2020-07-17 设计创作，主要内容包括：本发明公开了水稻组蛋白去甲基化酶JMJ708在水稻育种中的应用,属于农作物育种技术领域。通过本发明对于水稻组蛋白去甲基化酶JMJ708对水稻抗倒伏的调控研究,有利于选育出抗倒伏品种,增加粮食生产的品系,对解决目前生产上低产,易倒伏的问题可以很好的解答,能够为农业生产,粮食安全,农民增收提供保障。(The invention discloses application of rice histone demethylase JMJ708 in rice breeding, and belongs to the technical field of crop breeding. The regulation and control research of the rice histone demethylase JMJ708 on the lodging resistance of the rice is beneficial to breeding lodging-resistant varieties and increasing the variety of grain production, can well solve the problems of low yield and easy lodging in the prior production, and can provide guarantee for agricultural production, grain safety and income increase of farmers.)

1. The application of the rice histone demethylase JMJ708 and the coding gene thereof in rice breeding is shown in SEQ ID No. 1.

2. Use according to claim 1, characterized in that: the rice histone demethylase JMJ708 and the coding gene thereof are used for regulating and controlling the plant height.

3. Use according to claim 1, characterized in that: the rice histone demethylase JMJ708 and the coding gene thereof are used for regulating and controlling the tillering number.

4. Use according to claim 1, characterized in that: the rice histone demethylase JMJ708 and the coding gene thereof are used for regulating and controlling seed shedding.

5. Use according to claim 1, characterized in that: the rice histone demethylase JMJ708 and the coding gene thereof are used for regulating and controlling the brittleness of leaves, leaf sheaths and stalks.

Technical Field

The invention belongs to the technical field of crop breeding, and particularly relates to application of rice histone demethylase JMJ708 in rice breeding.

Background

In recent years, the rapid development of the research of functional genomes of rice has identified a large number of genes with important utilization value for the genetic breeding of rice in China, and the breeding of rice is advancing to the new era of design breeding. The innovative development of rice breeding provides important guarantee for the safety of food in China, and the improvement of yield per unit is still the main attack direction of rice breeding in China in a future period. Therefore, the discovery, cloning and identification of new important agronomic character regulation genes of rice are still important, elements are provided for the design and breeding, and the requirements of the precision, datamation and intelligent strategies of rice breeding in a new period are met.

The rice histone demethylase family has 20 genes which are respectivelyOsJMJ701、OsJMJ702、OsJMJ703、OsJMJ704、OsJMJ705、OsJMJ706、OsJMJ707、OsJMJ708、OsJMJ709、OsJMJ710、OsJMJ711、OsJMJ712、OsJMJ713、OsJMJ714、OsJMJ715、OsJMJ716、OsJMJ717、OsJMJ718、OsJMJ719、OsJMJ720. It has been reported that members of the rice histone demethylase family play an important role in the development and flowering of rice organs, among them,OsJMJ703can regulate and control the stem elongation of rice and the activity of retrotransposon.

At present, nothing is yet relatedOsJMJ708The reports on regulating and controlling rice development and enhancing rice lodging resistance.

Disclosure of Invention

The invention aims to provide rice histone demethylase JMJ708 (OsJMJ708) The application in rice breeding provides a new resource and a research method for rice breeding.

The invention constructs OsJMJ708 knockout mutant by utilizing a T-DNA insertion method, wherein T-DNA is inserted intoOsJMJ708Knocking out OsJMJ708 in rice on the 6 th exon of the sequence; simultaneously also utilizes RNA interference technology pairOsJMJ708The 3 rd exon and the 5 th exon of the sequence of. Thereafter, the mutants were subjected to a related study.

Has the advantages that:

the invention researches the function of the rice histone demethylase JMJ708 in rice development and lodging resistance in detail. The research of the invention shows that the rice histone demethylase JMJ708 is an important factor for regulating and controlling the plant height, the tillering number, the stem and leaf fragmentity and the seed shedding.

The regulation and control research of the rice histone demethylase JMJ708 on the lodging resistance of the rice is beneficial to breeding lodging-resistant varieties and increasing the variety of grain production, can well solve the problems of low yield and easy lodging in the prior production, and can provide guarantee for agricultural production, grain safety and income increase of farmers.

Drawings

FIG. 1 is a drawing ofOsJMJ708T-DNA insertion mutant construction and expression pattern results. Wherein: a is a T-DNA insertionOsJMJ708Position of (a) and position of each primer: TR390R, TR390LS and NGUS1 are used as primers for genotype identification, and RTF and RTR are used for detectionOsJMJ708A primer for gene expression level; b is the genotype identification of 23 transgenic plants; c isOsJMJ708The expression level in each genotype plant; d isOsJMJ708Expression levels in different tissues and organs.

FIG. 2 isOsJMJ708Knocking out the phenotype result of the homozygous mutant plant. Wherein: a is the phenotype of the seedling 5 days after budding; b is short-day greenhouse condition, and the heading stage phenotype; c is field condition, mature phenotype; d is the length of different internodes and rice ears; e is a rice ear phenotype; f is leaf fragility phenotype; g is the brittle phenotype of the second internode of the stalk.

FIG. 3 is a drawing showingOsJMJ708Content of each component of mutant cell wall. Wherein: A-C is the content of cellulose in the 2 nd internode, leaf sheath and leaf blade of the stem; D-E is the cellulose dyeing in the 2 nd internode (D) and the leaf sheath (E) of the stem; F-H is the content of lignin in the 2 nd internode, leaf sheath and leaf blade of the stem; i is gas chromatography analysis of the content of each monosaccharide in the cell wall.

FIG. 4 shows the observation of wild type andOsJMJ708results of mutant cell walls. Wherein: a is wild cell wall; B. c is the wild type primary cell wall (C is a partial enlargement of B); d isOsJMJ708A mutant cell wall; E. f isOsJMJ708Mutant primary cell wall (F is a local enlargement of E); PCW is primary cell wall; SCW is the secondary cell wall.

FIG. 5 is a drawing showingOsJMJ708Phenotypic characterization of RNAi transgenic plants. Wherein: a is OsJMJ708 at RExpression levels in NAi transgenic plants and wild-type; b is the friable phenotype of OsJMJ708-RNAi transgenic plants and wild type 2 nd leaf.

Detailed Description

The present invention will be described in detail below by way of examples with reference to the accompanying drawings, but the present invention is not limited thereto and is only described by way of example.