阅读说明：本技术 一种用于微全分析系统芯片集成化进样系统及进样方法 (Chip integrated sample injection system and method for micro total analysis system ) 是由 张贺 杨爽 揣荣岩 李新 张英杰 于 2020-06-04 设计创作，主要内容包括：本发明涉及一种用于微全分析系统芯片的集成化进样系统,包括进样控制模块、机械支撑模块和进样连接模块,进样连接模块和进样控制模块通过机械支撑模块支撑,所述进样连接模块包括有微全分析系统芯片和进液装置,进液装置的出液口与微全分析系统预留的进液口连接,所述进样控制模块包括有能够控制进液装置进液的动力装置。本系统可进一步提高芯片的实时性检测能力,有利于芯片应用领域的拓展和商品化进程。(The invention relates to an integrated sample feeding system for a micro total analysis system chip, which comprises a sample feeding control module, a mechanical support module and a sample feeding connection module, wherein the sample feeding connection module and the sample feeding control module are supported by the mechanical support module, the sample feeding connection module comprises a micro total analysis system chip and a liquid inlet device, a liquid outlet of the liquid inlet device is connected with a liquid inlet reserved in the micro total analysis system, and the sample feeding control module comprises a power device capable of controlling liquid inlet of the liquid inlet device. The system can further improve the real-time detection capability of the chip, and is beneficial to the expansion of the application field of the chip and the commercialization process.)

1. The utility model provides a sample introduction system integrates for little total analysis system chip which characterized in that: including advancing kind control module, mechanical support module and kind link module, advance kind link module and advance kind control module and pass through the mechanical support module support, advance kind link module including little complete analysis system chip (12) and inlet means, inlet means's liquid outlet is connected with the inlet that little complete analysis system reserved, advance kind control module including the power device that can control the inlet means feed liquor.

2. The integrated sample introduction system for the micro total analysis system chip according to claim 1, wherein: the power device is a stepping motor (2), a stepping motor rotating shaft (202) of the stepping motor (2) is connected with a driving lead screw (7), the driving lead screw (7) is in threaded fit with a lead screw nut (6), the lead screw nut (6) is fixed together with a sliding support arm (303), and the sliding support arm (303) is connected together with a propulsion liquid inlet component of a liquid inlet device.

3. The integrated sample introduction system for the micro total analysis system chip according to claim 1 or 2, wherein: the liquid inlet device is an injector, the injector comprises an injector shell (1301) and an injector piston (1302) serving as a liquid inlet propelling part, the injector shell (1301) is fixed with the mechanical support module, and a liquid outlet of the injector shell (1301) is connected with a liquid inlet reserved on the micro total analysis system chip (12).

4. The integrated sample introduction system for the micro total analysis system chip according to claim 3, wherein: the micro total analysis system chip (12) is positioned in the micro total analysis system chip clamp (11), the micro total analysis system chip clamp (11) comprises a micro total analysis system chip clamp base (1102) and a clamp cover plate (1101), the micro total analysis system chip (12) is fixed in the micro total analysis system chip clamp base (1102) in a pressurizing mode through the clamp cover plate (1101), a liquid outlet rubber plug (901) penetrates through a clamp through hole (1103) in the micro total analysis system chip clamp cover plate (1101) to be connected with a liquid outlet of the micro total analysis system chip (12), and a liquid inlet rubber plug (902) penetrates through a clamp through hole (1103) in the micro total analysis system chip clamp base (1102) to be connected with a liquid inlet reserved on the micro total analysis system chip (12).

5. The integrated sample introduction system for the micro total analysis system chip according to claim 3, wherein: the mechanical support module comprises a base (8) and a plurality of support arms, the support arms comprise fixed support arms and sliding support arms (303), the sliding support arms (303) can slide on the base (8), and the fixed support arms are fixed with a micro total analysis system chip (12), an injector shell (1301) and a stepping motor (2).

6. The integrated sample introduction system for the micro total analysis system chip according to claim 2, wherein: the sliding support arm (303) comprises an extrusion fixing block (3031) and a support fixing block (3032), the extrusion fixing block (3031) and the support fixing block (3032) are connected together through a bolt e (405), and an injector clamping piston hole (3034) for placing an injector piston (1302) is arranged between the extrusion fixing block (3031) and the support fixing block (3032).

7. The integrated sample introduction system for the micro total analysis system chip according to claim 6, wherein: the syringe clamping piston hole (3034) is provided with a plurality of clamping parts.

8. The integrated sample introduction system for the micro total analysis system chip according to claim 2, wherein: the sample injection control module further comprises a motor interface (102), a liquid crystal display (103), a motor drive circuit (104), a key (105), a power module (106) and an embedded processor (107), the power module (106) comprises a chip power supply (1061) and a motor power supply (1062) and is provided with the power interface (101), the embedded processor (107) of the sample injection control module is connected with the key (105), the embedded processor (107) is further connected with the liquid crystal display (103), the embedded processor (107) is connected with the motor drive circuit (104), the motor drive circuit (104) is connected with the stepping motor (2) through the motor interface (102), the motor power supply (1062) is connected with the motor drive circuit (104), and the chip power supply (1061) is connected with the liquid crystal display (103) and the embedded processor (107).

9. The integrated sample introduction system for the micro total analysis system chip according to claim 8, wherein: the liquid crystal display screen (103), the motor driving circuit (104), the power supply module (106) and the embedded processor (107) are sealed in the protective shell (1), and the power supply interface (101), the motor interface (102) and the keys (105) are arranged outside the protective shell (1).

10. An integrated sample introduction method of a micro total analysis system chip is characterized in that: an embedded processor (107) of the sample injection control module receives external instructions through a key (105) and displays the instructions on a liquid crystal display screen (103); the motor driving circuit (104) controls the stepping motor (2) to complete corresponding operation through the motor interface (102) and the motor lead (201) according to the external instruction analyzed by the embedded processor (107); when the rotating shaft (202) of the stepping motor rotates, the driving lead screw (7) connected with the rotating shaft is driven to rotate along with the rotating shaft, the rotating driving lead screw (7) drives the sliding supporting arm (303) to slowly move through the lead screw nut (6), and at the moment, the injector piston (1302) fixed together with the sliding supporting arm (303) also slowly moves along with the sliding supporting arm; the injector piston (1302) pushes the sample in the injector shell (1301) to enter the micro total analysis system chip (12), thereby realizing the sample injection of the micro total analysis system chip.

Technical Field

The invention belongs to the field of Micro-electro Mechanical Systems (MEMS), and particularly relates to an integrated sample introduction system and a sample introduction method for a Micro total analysis system chip.

Background

The micro total analysis system chip is a micro structure which is manufactured and designed by the MEMS technology on a substrate material with limited volume so as to realize the rapid detection of trace samples. The micro total analysis system has small volume and multiple functions, can highly integrate the analysis steps such as sample introduction, mixing, purification and the like which are usually finished in a laboratory, is a cross-combined product of multiple subjects such as chemistry, hydromechanics, microelectronics and the like, and is also an important development direction of portable and intelligent detection. At present, the chip of micro total analysis system has become one of the important platforms for the research of life science, pharmacy, chemistry and chemical engineering on molecular level. With the continuous expansion of research and application fields, especially for revealing intermolecular and intercellular internal interactions, the characteristic dimensions of chip structures have further been reduced to submicron and even nanometer, which poses more difficult challenges for fluid driving and control.

At present, the most extensive electroosmosis driving mode is applied to a micro total analysis system chip, although stable and accurate driving force can be provided for micro-nano fluid, the driving voltage is up to 150-200V/cm, the large driving voltage not only causes the overheating of a driving electrode to generate bubbles, but also needs a large-volume linear power supply as a guarantee, and the micro total analysis system chip is not suitable for the large trend of system miniaturization and portability; although the magnetic driving mode has unique advantages in the aspect of biochemical nondestructive detection, the types of fluids capable of being driven are few, so that the application range of the chip is limited; the centrifugal force driving mode is to expose the whole chip under the action of centrifugal force, so that the on-chip functional module which does not need large driving force is influenced.

The micro-injection type driving mode adopted by the invention belongs to one of pressure driving, and is applied to occasions needing accurate, timed and quantitative perfusion or extraction of microfluid, such as clinical medical treatment, biochemical tests and the like at present. However, the existing commercial micro-injection pump does not provide a customized design for a new application field with a broad prospect, namely a micro total analysis system chip, so that the existing commercial micro-injection pump cannot meet the requirements of chip miniaturization, integration and portable detection.

Disclosure of Invention

Object of the Invention

The invention provides an integrated sample introduction system for a chip of a micro total analysis system, which can solve the problems of large volume, complex assembly, low portability and the like of the existing chip sample introduction device of the micro total analysis system.

Technical scheme

The utility model provides a kind system of advancing that integrates for little complete analysis system chip, includes and advances kind control module, mechanical support module and advance kind link module, advances kind link module and advances kind control module and support through mechanical support module, advance kind link module including little complete analysis system chip and inlet means, inlet means's liquid outlet and the inlet that little complete analysis system reserved are connected, advance kind control module including the power device that can control the inlet means feed liquor.

The power device is a stepping motor, a rotating shaft of the stepping motor is connected with a driving lead screw, the driving lead screw is in threaded fit with a lead screw nut, the lead screw nut is fixed with the sliding support arm, and the sliding support arm is connected with a propulsion liquid inlet component of the liquid inlet device.

The liquid inlet device is an injector, the injector comprises an injector shell and an injector piston used as a liquid inlet propelling part, the injector shell is fixed with the mechanical support module, and a liquid outlet of the injector shell is connected with a liquid inlet reserved on the chip of the micro total analysis system.

The micro total analysis system chip is positioned in the micro total analysis system chip clamp, the micro total analysis system chip clamp comprises a micro total analysis system chip clamp base and a clamp cover plate, the clamp cover plate presses and fixes the micro total analysis system chip in the micro total analysis system chip clamp base, a liquid outlet rubber plug penetrates through a clamp through hole on the micro total analysis system chip clamp cover plate to be connected with a liquid outlet of the micro total analysis system chip, and a liquid inlet rubber plug penetrates through a clamp through hole on the micro total analysis system chip clamp base to be connected with a reserved liquid inlet on the micro total analysis system chip.

The mechanical support module comprises a base and a plurality of support arms, the support arms comprise a fixed support arm and a sliding support arm, the sliding support arm can slide on the base, and the fixed support arm is fixed with a micro total analysis system chip, an injector shell and a stepping motor.

The sliding support arm comprises an extrusion fixing block and a support fixing block, the extrusion fixing block and the support fixing block are connected together through a bolt e, and an injector clamping piston hole used for placing an injector piston is formed between the extrusion fixing block and the support fixing block.

The syringe clamping piston hole is provided with a plurality of clamping parts.

The sample injection control module further comprises a motor interface, a liquid crystal display screen, a motor driving circuit, a button, a power module and an embedded processor, the power module comprises a chip power supply and a motor power supply and is provided with the power interface, the embedded processor of the sample injection control module is connected with the button, the embedded processor is further connected with the liquid crystal display screen, the embedded processor is connected with the motor driving circuit, the motor driving circuit is connected with a stepping motor through the motor interface, the motor power supply is connected with the motor driving circuit, and the chip power supply is connected with the liquid crystal display screen and the embedded processor.

The liquid crystal display screen, the motor driving circuit, the power module and the embedded processor are sealed in the protective shell, and the power interface, the motor interface and the keys are arranged outside the protective shell.

An integrated sample introduction method of a micro total analysis system chip comprises the following steps: an embedded processor of the sample injection control module receives external instructions through keys and displays the instructions on a liquid crystal display screen; the motor driving circuit controls the stepping motor to complete corresponding operation through the motor interface and the motor lead according to the external instruction analyzed by the embedded processor; when the rotating shaft of the stepping motor rotates, the driving screw rod connected with the rotating shaft is driven to rotate along with the rotating shaft, the rotating driving screw rod drives the sliding support arm to slowly move through the screw rod nut, and at the moment, the injector piston fixed with the sliding support arm also slowly moves along with the sliding support arm; the injector piston pushes the sample in the injector shell to enter the micro total analysis system chip, thereby realizing the sample introduction of the micro total analysis system chip.

Advantages and effects

The invention provides an integrated sample introduction system for a micro total analysis system chip, which is intended to provide efficient and stable fluid drive and control for the micro total analysis system chip with unified standard after customized design; the device can provide operations of sample introduction, flushing, sample changing and the like with high precision, quick response and small volume for a multifunctional and multipurpose micro total analysis system chip. The system is integrated with a visual operation interface, can set parameters such as sample volume, speed and the like, and provides a multi-path sample introduction function according to the needs of users. In addition, the system integrates a standardized chip clamp of the micro total analysis system and a liquid inlet interface and a liquid outlet interface, thereby providing convenient conditions for users to quickly detect. The invention can further improve the real-time detection capability of the chip and is beneficial to the expansion of the application field of the chip and the commercialization process.

Drawings

FIG. 1 is a block diagram of an integrated sample injection system for a micro total analysis system chip;

FIG. 2 is a schematic diagram of a sample injection control module;

FIG. 3 is a schematic diagram of a sample injection control module circuit;

FIG. 4 is a view showing the structure of a base;

fig. 5, fixed support arm a;

fig. 6, fixed support arm b;

FIG. 7, sliding support arm;

fig. 8, fixed support arm c;

fig. 9, fixed support arm d;

FIG. 10, a reducer casing configuration;

FIG. 11, lead screw nut configuration;

FIG. 12 shows a chip fixture structure of the micro total analysis system;

FIG. 13 is an assembled side view of an integrated sample injection system for a micro total analysis system chip;

FIG. 14 is a top view of an integrated sample injection system for a micro total analysis system chip;

FIG. 15 is an assembly view of the variable diameter sleeve;

FIG. 16, lead screw nut assembly view;

FIG. 17 is an assembly view of a chip holder for micro total analysis system.

The reference signs are: 1. the electronic device comprises a protective shell, 101 power interfaces, 102 motor interfaces, 103 liquid crystal display screens, 104 motor driving circuits, 105 buttons, 106 power supplies, 107 embedded processors, 2 stepping motors, 201 motor leads, 202 stepping motor rotating shafts, 203 rotating shaft fixing holes, 301 fixing supporting arms a, 302 fixing supporting arms b, 303 sliding supporting arms, 304 fixing supporting arms c, 305 fixing supporting arms d, 401 bolts a, 402 bolts b, 403 bolts c, 404 bolts d, 405 bolts e, 406 bolts f, 407 bolts g, 5 reducing sleeves, 501 rotating shaft fixing holes, 502 lead screw fixing holes, 6 lead screw nuts, 601 lead screw holes, 602 nut fixing holes, 603 fixing plates, 7 driving lead screws, 701 lead screw fixing holes, 8 bases, 801 bottom fixing holes, 802 side fixing holes, a sliding supporting arm, 304 fixing supporting arms c, 305, fixing supporting arms d, 401 fixing bolts a lead screw, and a nut fixing hole for the lead screw, 901. The micro total analysis system chip clamp comprises a liquid outlet rubber plug, 902, a liquid inlet rubber plug, 10, a hose, 11, a micro total analysis system chip clamp, 1101, a micro total analysis system chip clamp cover plate, 1102, a micro total analysis system chip clamp base, 1103, a clamp through hole, 12, a micro total analysis system chip, 1301, an injector shell, 1302, an injector piston, 3011, a protective shell fixing hole, 3012, a motor fixing hole, 3013, a rotating shaft-lead screw through hole, 3014, a side-bottom fixing hole, 3021, a bottom-bottom fixing hole, 3031, an extrusion fixing block, 3032, a support fixing block, 3033, a bolt e through hole, 3034, an injector clamp piston hole, 3035, a lead screw nut through hole, 3036, a lead screw nut fixing through hole, 3041, a clamp fixing hole, 3042, an injector fixing hole, 3043, a connection-fixing hole, 4061, a clamp bolt, 4062 and a clamp nut.

Detailed Description

The technical solutions of the present invention are further described in detail with reference to the drawings and the specific embodiments, and all the portions of the present invention which are not described in detail are the prior art.

As shown in fig. 13, fig. 14, fig. 15, fig. 16 and fig. 17, an integrated sample injection system for a micro total analysis system chip comprises a sample injection control module, a mechanical support module and a sample injection connection module, wherein the sample injection connection module and the sample injection control module are supported by the mechanical support module, the sample injection connection module comprises a micro total analysis system chip 12 and a liquid inlet device, a liquid outlet of the liquid inlet device is connected with a liquid inlet reserved in the micro total analysis system, and the sample injection control module comprises a power device capable of controlling the liquid inlet device to feed liquid. The power device is a stepping motor 2, a stepping motor rotating shaft 202 of the stepping motor 2 is connected with a driving screw 7 through a reducing sleeve 5 fixed by a bolt g 407, the driving screw 7 is in threaded fit with a screw nut 6, the screw nut 6 is fixed with a sliding support arm 303, and the sliding support arm 303 is connected with a propulsion liquid inlet component of the liquid inlet device. The sliding support arm 303 comprises an extrusion fixing block 3031 and a support fixing block 3032, the top of the extrusion fixing block 3031 is provided with 2 jack screw bolt through holes 3033 for jack screw bolts 405 to pass through, the extrusion fixing block 3031 and the support fixing block 3032 are connected together through bolts e405, and an injector clamp piston hole 3034 for placing an injector piston 1302 is arranged between the extrusion fixing block 3031 and the support fixing block 3032. The liquid inlet device is an injector, the injector comprises an injector shell 1301 and an injector piston 1302 serving as a propulsion liquid inlet component, the injector shell 1301 is fixed with the mechanical support module, a liquid outlet of the injector shell 1301 is connected with one end of a hose 10, the other end of the hose 10 is connected with a liquid inlet rubber plug 902, and the other end of the liquid inlet rubber plug 902 is connected with a liquid inlet reserved on the micro total analysis system chip 12. As shown in fig. 14, the sample injection control module further includes a motor interface 102, a liquid crystal display screen 103, a motor driving circuit 104, a key 105, a power module 106 and an embedded processor 107, the power module 106 includes a chip power supply 1061 and a motor power supply 1062 and is provided with a power interface 101, the embedded processor 107 of the sample injection control module is connected with the key 105 to receive external instructions, the embedded processor 107 is further connected with the liquid crystal display screen 103, and the instructions are displayed on the liquid crystal display screen 103; the embedded processor 107 is connected with a motor driving circuit 104, the motor driving circuit 104 is connected with the stepping motor 2 through a motor interface 102, a motor power supply 1062 is connected with the motor driving circuit 104, and a chip power supply 1061 is connected with the liquid crystal display 103 and the embedded processor 107. The micro total analysis system chip 12 is located in the micro total analysis system chip fixture 11, the micro total analysis system chip fixture 11 includes a micro total analysis system chip fixture base 1102 and a fixture cover plate 1101 (see fig. 12), the fixture cover plate 1101 fixes the micro total analysis system chip 12 in the micro total analysis system chip fixture base 1102 under pressure, the liquid outlet rubber plug 901 passes through the fixture through hole 1103 on the micro total analysis system chip fixture cover plate 1101 to connect with the liquid outlet of the micro total analysis system chip 12, and the liquid inlet rubber plug 902 passes through the fixture through hole 1103 of the micro total analysis system chip fixture base 1102 to connect with the liquid inlet reserved on the micro total analysis system chip 12.

In fig. 4, 7 and 13, the mechanical support module includes a base 8 and a plurality of support arms, the support arms include a fixed support arm and a sliding support arm 303, the sliding support arm 303 can slide on the base 8, and the fixed support arm is fixed with a micro total analysis system chip 12, a syringe housing 1301 and a stepping motor 2. Preferably, the syringe catch piston bore 3034 has a plurality of catch locations. The liquid crystal display screen 103, the motor driving circuit 104, the power supply module 106 and the embedded processor 107 are sealed in the protective shell 1, the protective shell 1 is preferably made of organic glass material, and the power supply interface 101, the motor interface 102 and the keys 105 are arranged outside the protective shell 1.

An integrated sample introduction method of a micro total analysis system chip, an embedded processor 107 of a sample introduction control module receives external instructions through a key 105 and displays the instructions on a liquid crystal display screen 103; the motor driving circuit 104 controls the stepping motor 2 to complete corresponding operations through the motor interface 102 and the motor lead 201 according to the external instruction analyzed by the embedded processor 107; in fig. 10, when the stepping motor shaft 202 rotates, the driving screw 7 connected with the stepping motor shaft is driven by the reducer sleeve 5 to rotate (see fig. 13), the rotating driving screw 7 drives the sliding support arm 303 to move slowly by the screw nut 6 (see fig. 7/13), and at this time, the syringe piston 1302 fixed with the sliding support arm 303 also moves slowly together; the syringe piston 1302 pushes the sample in the syringe housing 1301 to enter the micro total analysis system chip 12 through the hose 10 and the liquid inlet plug 902, so that the sample introduction of the micro total analysis system chip is realized, and the sample which is analyzed and detected is discharged through the liquid outlet plug 901.

FIG. 1 is a block diagram of an integrated sample injection system for a chip of a micro total analysis system. An integrated sample introduction system for a micro total analysis system chip mainly comprises: the sampling control module, the mechanical support module and the sampling connection module. Wherein advance a kind control module and mainly include: the system comprises a liquid crystal display screen 103, a motor driving circuit 104, a key 105, a power supply 106, an embedded processor 107 and a stepping motor 2; wherein the mechanical support module mainly comprises: the sample injection connection module mainly comprises a micro total analysis system chip 12, a micro total analysis system chip clamp 11, rubber plugs (a liquid outlet rubber plug 901 and a liquid inlet rubber plug 902), a hose 10 and an injector (an injector shell 1301 and an injector piston 1302).

As shown in fig. 2, the lcd 103, the motor driving circuit 104, the power module 106 and the embedded processor 107 in the sample injection control module are sealed in the organic glass protective case 1, and only the power interface 101, the motor interface 102 and the keys 105 which are subjected to waterproof treatment are in contact with the outside, so as to avoid short circuit when encountering water during the detection process.

As shown in fig. 3, the power module 106 in the sample injection control module is composed of a chip power supply 1061 and a motor power supply 1062, and can be connected to an external power supply through a power interface 101; the chip power supply 1061 converts the input voltage of the external power supply into a 3.3V dc voltage, and is connected to the VCC3.3 and GND pins of the lcd 103 and the embedded processor 107 through the VCC3.3 and GND pins, respectively, to provide the power supply voltage for the chips of the lcd 1602 and the embedded processor 107; the motor power supply 1062 converts the input voltage of the external power supply into a 12V dc voltage, and connects the VCC12 and GND pins of the motor power supply 1062 with the VCC12 and GND pins of the stepping motor 2 through the motor interface 102 and with the motor driver 104, and the motor power supply 1062 provides the power supply voltage for the stepping motor 2; the embedded processor 107 is connected with the pins P14, P13, P12, P11, P10, P9, P8, P7, P6, P5 and P4 corresponding to the liquid crystal display screen 103 through the pins P1.5, P1.6, P1.7, P2.0, P2.1, P2.2, P2.3, P2.4, P2.5, P2.6 and P2.7, and provides display driving and display contents for the liquid crystal display screen 103; the embedded processor 107 is connected with pins P14, P15 and P20 of the motor driving circuit 104 through pins P1.2, P1.3 and P1.4 to provide driving control signals for the motor driving circuit 104; pins P0.6, P0.7, P1.0, P1.1, P3.1, P3.2, P3.3 and P3.4 of the embedded processor 107 are connected with a key 105 of Risym6 × 6 × 5; after the motor driving circuit 104 converts the driving control signal from the embedded processor 107 into a signal format recognizable for the stepping motor, pins P23, P24, P32, P33, P34, P35, P43 and P44 are connected to pins 1ch OUTA, 2ch OUTA, 1ch OUTB and 2ch OUTB of the stepping motor 2 through the motor interface 102, so as to realize the driving control of the stepping motor 2. The stepping motor lead 201 is a lead for connecting the stepping motor 2 with the motor interface 102.

Controlling the stepping motor 2 to complete corresponding operation through the motor interface 102 and the motor lead 201 according to the external instruction analyzed by the embedded processor 107; when the rotating shaft 202 of the stepping motor rotates, the driving lead screw 7 connected with the reducing sleeve 5 rotates along with the rotating shaft, the rotating driving lead screw 7 drives the sliding support arm 303 to move slowly through the lead screw nut 6, and at the moment, the injector piston 1302 fixed on the injector piston clamping hole 3034 also moves slowly along with the sliding support arm, so that the sample introduction of the micro total analysis system chip 12 is realized.

As shown in fig. 4, the base 8 of the mechanical support module is provided with 4 bottom fixing holes 801 and 4 side fixing holes 802 for fixing the support arm.

As shown in fig. 5, the top of the fixing support arm a 301 in the mechanical support module has 2 protective casing fixing holes 3011 for fixing the protective casing 1; the side surface is provided with 4 motor fixing holes 3012 for fixing the stepping motor 2, 1 rotating shaft-lead screw through hole 3013 for supporting the rotating shaft 202 of the stepping motor and the driving lead screw 7, and 2 side-bottom fixing holes 3014 for connecting and fixing with the base 8.

As shown in fig. 6, the top of the fixed support arm b 302 in the mechanical support module has 2 protective case fixing holes 3011 for fixing the organic glass protective case 1; the side surface is provided with 4 motor fixing holes 3012 for fixing the stepping motor 2 and 1 rotating shaft-lead screw through hole 3013 for supporting the rotating shaft 202 of the stepping motor and the driving lead screw 7; the bottom surface has 2 bottom-bottom fixing holes 3021 for connecting and fixing with the base 8.

As shown in fig. 7, the sliding support arm 303 of the mechanical support module is composed of two parts, namely a pressing fixing block 3031 and a support fixing block 3032. The top of the extrusion fixing block 3031 is provided with 2 bolt e through holes 3033 for bolts e405 to pass through; the top of the supporting fixing block 3032 is provided with 1 syringe clamping piston hole 3034 for placing the syringe piston 1302, and the side surface is provided with 1 lead screw nut through hole 3035 for installing the lead screw nut 6 and 2 lead screw nut fixing through holes 3036 for fixing the lead screw nut 6.

As shown in fig. 8, the top of the fixed support arm c 304 in the mechanical support module has 2 fixture fixing holes 3041 for fixing the micro total analysis system chip fixture base 1102; the side surface of the syringe is provided with 4 connecting-fixing holes 3043 for fixing the support arm c 304 and the fixed support arm d305, 1 rotating shaft-lead screw through hole 3013 for supporting the rotating shaft 202 of the stepping motor and the driving lead screw 7 and 1 syringe fixing hole 3042 for supporting the syringe shell 1301; the bottom surface has 2 bottom-bottom fixing holes 3021 for connecting and fixing with the base 8.

As shown in fig. 9, the top of the fixed support arm d305 in the mechanical support module has 2 fixture fixing holes 3041 for fixing the micro total analysis system chip fixture base 1102; the side surface is provided with 4 connecting-fixing holes 3043 for fixing the supporting arm c 304 and the fixing supporting arm d305, 1 rotating shaft-lead screw through hole 3013 for supporting the rotating shaft 202 of the stepping motor and the driving lead screw 7, and 2 side-bottom fixing holes 3014 for connecting and fixing with the base 8.

As shown in fig. 10, the reducer sleeve 5 is a hollow reducing cylinder, and has a shaft fixing hole 501 for mounting the stepping motor shaft 202 at a smaller end thereof, and a screw fixing hole 502 for fixing the drive screw 7 at a larger end thereof.

As shown in fig. 11, one end of the screw nut 6 is an elliptical fixing piece 603, the other end is a circular screw hole 601, and 2 nut fixing holes 602 for fixing the screw nut 6 are provided in the fixing piece 603.

As shown in fig. 12, the micro total analysis system chip holder 11 is composed of a micro total analysis system chip holder cover 1101 and a micro total analysis system chip holder base 11022. Fixture through holes 1103 for chip pressurization, fixture fixing, and liquid inlet/outlet mounting are uniformly arranged on the micro total analysis system chip fixture cover 1101 and the micro total analysis system chip fixture base 1102.

As shown in fig. 15 and 16, the drive screw 7 is provided with a screw fixing hole 701 for fixing to the adapter sleeve 5, and the stepping motor shaft is provided with a shaft fixing hole 203 for fixing to the adapter sleeve 5.

As shown in fig. 17, the micro total analysis system fixture mount 1102 is connected to the fixed support arm c 304 and the fixed support arm d305 by the mini bolts a 401; one end of the liquid inlet rubber plug 902 passes through a through hole 1103 on the chip fixture base 1102 of the micro total analysis system and is connected with a liquid inlet reserved on the chip 12 of the micro total analysis system; the outlet plug 901 passes through a fixture through hole 1103 on the fixture cover plate 1101 of the micro total analysis system chip and is connected to the outlet of the micro total analysis system chip 12.

The fixed support arm c 304 and the fixed support arm d305 are fixed strongly by a bolt b 402 passing through both of them; the fixed support arm a 301, the fixed support arm b 302, the fixed support arm c 304 and the fixed support arm d305 are fixedly connected with the base 8 through a bolt c 403; the screw nut 6 and the support fixing block 3032 are fixedly connected through a bolt d 404; a bolt e405 penetrates through the extrusion fixing block 3031 and the support fixing block 3032 to connect the two together; the bolt f 406 penetrates through the cover plate 1101 of the micro total analysis system chip clamp and a clamp through hole 1103 which is not blocked by the micro total analysis system chip 12 on the base 1102 of the micro total analysis system chip clamp, and then is screwed with a clamp nut 4062 to provide pressurization fixation for the tightness of the chip; the reducing sleeve 5 and the driving screw 7 are fixed by a bolt g 407, and the reducing sleeve 5 and the stepping motor shaft 202 are fixed by the bolt g 407.

The design principle of the invention is as follows:

the invention selects the C8051F310 chip as the embedded processor. The C8051F310 chip has 32 pins, belongs to a small-sized and exterior microcontroller, and integrates an 8051CPU with the operation speed of 25MIPS, a 16KB Flash memory, a liquid crystal driving circuit and a plurality of I/O pins.

In the embodiment, AMS1117 and LM2596 are selected as core chips of a chip power supply and a motor power supply. The AMS1117 is a three-terminal linear voltage stabilizing circuit chip with positive voltage output and low voltage difference, and is divided into two major types, namely an adjustable version and a plurality of fixed voltage versions. AMS1117 outputs a 1A current with a typical input to output voltage difference of 1.8V, and the minimum voltage difference is guaranteed to not exceed 1.3V and gradually decreases with decreasing load current. The AMS1117 also integrates an overheating protection circuit and a current limiting circuit, and can further determine the stability of a chip and a power supply system. The invention selects a fixed voltage version with the output voltage of 3.3V and supplies power for circuits such as an embedded processor, a liquid crystal display screen, a motor drive and the like. LM2596 is a switching voltage regulator, has outstanding linearity and load regulation characteristics, and frequency compensation and fixed frequency generator are integrated in the chip, and the switching frequency can reach 150KHz, and the switching voltage regulator has a self-protection function. The invention selects a fixed output version with 12V output as a stepping motor and a driving circuit thereof for power supply.

The invention selects LV8731 as the core chip of the motor driving circuit. LV8731 is a double-channel H-bridge driving chip, the rated working voltage range is 9-32V, and the peak value of output current is 2.5A. LV8731 incorporates a PWMV current-controlled stepping motor drive unit that can operate in both DCM and STM modes and has output short-circuit protection and abnormal state warning functions. The invention selects a fixed output version with 12V working voltage output to provide a driving signal for the stepping motor.

The invention selects a 1602 type character liquid crystal display screen as a display chip. The 1602 type character LCD is a dot matrix LCD specially used for displaying alphanumerics and symbols, and can realize the display of 16 characters in 2 lines each. The specific pin description is shown in table 1.

Table 1: pin specification for 1602 type character lcd

The YH42BYGH60 type stepping motor is selected as a core device for driving control. A stepping motor (also called a pulse motor) is a motor that converts an electric signal into momentum by using a pulse signal as a control signal. When a pulse is input, he takes a step forward, and due to this particular form of operation, it is possible to achieve a higher precision control than a conventional motor that rotates at a high speed. Specific technical indexes of the YH42BYGH60 type stepping motor selected by the invention are detailed in Table 2:

table 2: YH42BYGH60 type stepping motor performance parameter table

The chip is used as a core device of a sample injection control module, the drive control function of a stepping motor is realized according to the connection mode shown in figure 3, and the integrated sample injection for the chip of the micro total analysis system is realized by combining the specific assembly of the mechanical support module and the sample injection connection module in the description of the attached drawings.

It should be understood that the above-mentioned embodiments of the present invention are only examples for clearly illustrating the present invention, and are not intended to limit the embodiments of the present invention, and it will be obvious to those skilled in the art that other variations or modifications may be made on the basis of the above description, and all embodiments may not be exhaustive, and all obvious variations or modifications belonging to the technical scheme of the present invention are within the scope of the present invention.