阅读说明：本技术 一种具有抗细菌粘附性能的表面微结构阵列及其成型方法 (Surface microstructure array with antibacterial adhesion performance and forming method thereof ) 是由 唐昆 李典雨 陈紫琳 易香怀 张明军 毛聪 胡永乐 于 2020-06-18 设计创作，主要内容包括：本发明公开了一种具有抗细菌粘附性能的表面微结构阵列及其成型方法。该表面微结构阵列的单个微结构为海胆亭结构,材料为光敏树脂,尺寸为微米级；微结构表面分布有纳米颗粒,与海胆亭结构形成微米/纳米复合结构；在成型件目标面上均布多个复合结构,即构成表面微结构阵列。该表面微结构阵列的成型方法,是利用一套电磁辅助振动的面投影微立体光刻系统,并遵循一定的步骤来完成其制备。本发明所述的表面微结构阵列的抗细菌粘附、防吸附、自清洁性能好,且微结构形貌尺寸可控；本发明所述成型方法通过电磁辅助振动装置的高频振动,减少纳米颗粒团聚,实现其均匀分布,提高成型精度与成型质量；成型系统移动平台可沿Y轴、X轴方向水平直线运动,扩大了成型层面面积与成型件尺寸,减少成型时间,提高成型效率。(The invention discloses a surface microstructure array with antibacterial adhesion performance and a forming method thereof. The single microstructure of the surface microstructure array is a sea urchin pavilion structure, the material is photosensitive resin, and the size is micron-sized; nano particles are distributed on the surface of the microstructure and form a micron/nano composite structure with the echinocandin structure; and uniformly distributing a plurality of composite structures on the target surface of the formed part to form a surface microstructure array. The forming method of the surface microstructure array utilizes a set of electromagnetic auxiliary vibration surface projection micro stereolithography system and follows certain steps to complete the preparation. The surface microstructure array has good antibacterial adhesion, adsorption resistance and self-cleaning performance, and the microstructure appearance and size are controllable; according to the forming method, the high-frequency vibration of the electromagnetic auxiliary vibration device is utilized, so that the agglomeration of nano particles is reduced, the uniform distribution of the nano particles is realized, and the forming precision and the forming quality are improved; the forming system moving platform can move horizontally and linearly along the directions of the Y axis and the X axis, so that the area of a forming layer and the size of a formed part are enlarged, the forming time is shortened, and the forming efficiency is improved.)

1. A surface microstructure array with antibacterial adhesion performance and a forming method thereof are characterized in that: the single microstructure of the surface microstructure array with the antibacterial adhesion performance is a sea urchin pavilion structure, the material is photosensitive resin, and the transverse dimension, the longitudinal dimension and the spacing of the photosensitive resin are all in a micron order; the sea urchin pavilion structure is in a regular ellipsoid shape, and a plurality of reinforcing ribs are annularly and uniformly distributed along the ellipsoid; the nano particles are distributed on the surface of the microstructure, so that a micron/nano composite structure is formed with the echinocandin structure; a plurality of the composite structures are uniformly distributed on the target surface of the formed part, so that a surface microstructure array is formed;

the forming method of the surface microstructure array with the antibacterial adhesion property utilizes a set of electromagnetic auxiliary vibration surface projection micro-stereolithography system and follows certain steps to complete the preparation;

the electromagnetic auxiliary vibration surface projection micro stereolithography system is provided with a marble base; the marble base is provided with a Y-axis slide rail, an X-axis slide rail and a moving platform from bottom to top respectively; the movable platform can horizontally and linearly move on the Y-axis slide rail and the X-axis slide rail along the Y-axis direction and the X-axis direction respectively;

two sides of the mobile platform are respectively provided with a Z1 shaft slide rail and a Z2 shaft slide rail;

a bottom plate is arranged on the Z1 shaft slide rail; the bottom plate can move up and down along the Z1 axis direction on a Z1 axis slide rail; the bottom plate is provided with an electromagnetic auxiliary vibration device and a resin tank from bottom to top respectively; the electromagnetic auxiliary vibration device comprises a magnetism isolating sleeve, an armature, a plate spring, an iron core and a coil; photosensitive resin is contained in the resin tank, and nanoparticles are doped in the photosensitive resin;

a forming platform is arranged on the Z2 shaft slide rail; the forming platform can move up and down along the Z2 axis direction on a Z2 axis slide rail; a formed part obtained by carrying out surface projection exposure and curing on photosensitive resin is supported on the forming platform;

an optical machine, a mask plate, a CCD camera, a 45-degree reflector and an objective lens are respectively arranged on the upper part of the resin groove from right to left;

and a computer PC is arranged on the right side of the resin tank.

2. In order to realize the formation of the surface microstructure array with the antibacterial adhesion property, the invention follows the following steps:

slicing a three-dimensional CAD model of a formed part with a surface microstructure array according to a certain thickness by adopting layering software, converting each layer of slices into a bitmap file, and inputting each bitmap file to the mask plate; the light beam emitted from the light machine is shaped by a mask plate pattern and contains image information of a layer surface to be cured, and then the light beam is reflected by the 45-degree reflector and focused by the objective lens and is exposed to the liquid surface of the photosensitive resin in the resin tank, so that the curing layer surface of a required pattern is obtained; after one layer surface is cured, the cured layer surface moves downwards along with the forming platform and sinks into the photosensitive resin, and the thickness of the resin covered on the cured layer surface is ensured to be exactly equal to the thickness of the next layer to be processed; repeating the above exposure process until all layers are exposed; the CCD camera is used for monitoring the forming and curing process of each layer;

in order to reduce the agglomeration phenomenon of the nano particles in the forming process, the lower part of the resin tank is provided with the electromagnetic auxiliary vibration device; the coil is electrified with half-wave rectified pulse current, the current magnetizes the iron core during positive half-wave to form an electromagnet and attract the armature iron, so that the resin tank is driven to move downwards; the current disappears during the negative half wave, and the resin groove returns to the original position under the action of the plate spring; the resin tank is circulated in such a way, so that the up-and-down vibration of the resin tank is realized; the magnetic isolation sleeve is arranged outside the electromagnetic auxiliary vibration device and used for preventing electromagnetic radiation from interfering with the outside;

the lower part of the moving platform is provided with an X-axis slide rail and a Y-axis slide rail, the resin tank can move along the X-axis direction and the Y-axis direction, and the exposure of different positions of the same resin liquid level can be realized, so that the splicing of a plurality of images on one plane is completed to form a larger layer;

the input of the mask plate pattern, the monitoring of the CCD camera, the electromagnetic auxiliary vibration device and the resin tank are driven by the bottom plate to move up and down on the Z1 shaft slide rail, the forming platform moves up and down on the Z2 shaft slide rail, and the moving platform moves on the Y shaft slide rail and the X shaft slide rail along the horizontal straight line of the Y shaft and the X shaft, which are all controlled by the computer PC;

after the preparation of the whole formed part is completed, the formed part is soaked in acetone solution, and micro corrosion is carried out on the formed part to expose the nano particles on the surface of the formed part, so that the micron/nano composite structure array with the antibacterial adhesion performance is formed.

Technical Field

The invention belongs to the technical field of surface modification and 3D printing and forming, and particularly relates to an antibacterial biological culture dish inner wall micro-structure array and a forming method thereof.

Background

The biofilm is a special growth state which is formed by microorganisms in order to adapt to the environment and is beneficial to the survival of the microorganisms, and is a multi-cell colony structure which is wrapped by extracellular mucilaginous substances secreted by the microorganisms and has high organization. The formation of a biofilm often occurs in the fields of medical treatment, bioengineering and the like, resulting in serious problems such as instrument pollution and the like. During biofilm formation, bacterial adhesion is the first step in its formation. Therefore, inhibition of bacterial adhesion is of great importance for controlling biofilm formation. The literature research shows that constructing the super-hydrophobic functional surface on the surface of the device is one of effective means for inhibiting the adhesion of bacteria. However, the current antibacterial efficiency of the antibacterial adhesion functional surface is not high, and the formation of the microstructure on the functional surface is difficult, which becomes a bottleneck restricting the application and development of the technology.

Disclosure of Invention

Aiming at the technical problems of low antibacterial efficiency, difficult forming of the microstructure of the functional surface and the like of the existing antibacterial adhesion functional surface, the invention aims to provide a surface microstructure array with antibacterial adhesion performance and a forming method thereof for resin-based medical instruments.

The technical scheme adopted by the invention for solving the technical problems is as follows: referring to the attached drawings 1-4, the single microstructure of the surface microstructure array with the antibacterial adhesion performance is a sea urchin pavilion structure, the material is photosensitive resin, and the transverse dimension, the longitudinal dimension and the spacing are in a micron order; the sea urchin pavilion structure is in a regular ellipsoid shape, and a plurality of reinforcing ribs are annularly and uniformly distributed along the ellipsoid; the nano particles are distributed on the surface of the microstructure, so that a micron/nano composite structure is formed with the echinocandin structure; and uniformly distributing a plurality of the composite structures on the target surface of the formed part to form a surface microstructure array.

The forming method of the surface microstructure array with the antibacterial adhesion property utilizes a set of electromagnetic auxiliary vibration surface projection micro-stereolithography system and follows certain steps to complete the preparation.

The electromagnetic auxiliary vibration surface projection micro stereolithography system is provided with a marble base. The marble base is provided with a Y-axis slide rail, an X-axis slide rail and a moving platform from bottom to top respectively. The movable platform can move on the Y-axis slide rail and the X-axis slide rail horizontally and linearly along the Y-axis direction and the X-axis direction respectively.

And Z1-axis slide rails and Z2-axis slide rails are respectively arranged on two sides of the moving platform.

And a bottom plate is arranged on the Z1 shaft slide rail. The bottom plate can move up and down along the Z1 axis direction on a Z1 axis slide rail. The bottom plate is provided with an electromagnetic auxiliary vibration device and a resin tank from bottom to top. The electromagnetic auxiliary vibration device comprises a magnetism isolating sleeve, an armature, a plate spring, an iron core and a coil. Photosensitive resin is contained in the resin tank, and 10% of nanoparticles are doped in the photosensitive resin.

And a forming platform is arranged on the Z2 shaft slide rail. The forming platform can move up and down along the Z2 axis direction on a Z2 axis slide rail. And a formed part obtained by carrying out surface projection exposure and curing on the photosensitive resin is supported on the forming platform.

And the upper part of the resin groove is respectively provided with an optical machine, a mask plate, a CCD camera, a 45-degree reflector and an objective lens from right to left.

And a computer PC is arranged on the right side of the resin tank.

In order to realize the formation of the surface microstructure array with the antibacterial adhesion property, the invention follows the following steps:

and slicing the three-dimensional CAD model of the formed part with the surface microstructure array according to a certain thickness by adopting layering software, converting each layer of slices into a bitmap file, and inputting each bitmap file to the mask plate. The light beam emitted from the light machine is shaped by the mask plate pattern and contains the image information of the layer surface to be cured, and then the light beam is reflected by the 45-degree reflector and focused by the objective lens and is exposed on the liquid surface of the photosensitive resin in the resin tank, so that the curing layer surface of the required pattern is obtained. After one layer surface is cured, the cured layer surface moves downwards along with the forming platform and sinks into the photosensitive resin, and the thickness of the resin covered on the cured layer surface is ensured to be exactly equal to the thickness of the next layer to be processed. Repeating the above exposure process until all layers are exposed. The CCD camera is used for monitoring the forming and curing process of each layer.

In order to reduce the agglomeration phenomenon of the nano particles in the forming process, the lower part of the resin tank is provided with the electromagnetic auxiliary vibration device. The coil is electrified with half-wave rectified pulse current, the current magnetizes the iron core during positive half-wave to form an electromagnet and attract the armature iron, so that the resin tank is driven to move downwards; the current disappears in the negative half wave, and the resin groove returns to the original position under the action of the plate spring. The resin tank is circulated in such a way, and the up-and-down vibration of the resin tank is realized. The electromagnetic auxiliary vibration device is externally provided with the magnetic isolation sleeve for preventing electromagnetic radiation from interfering with the outside.

The lower part of the moving platform is provided with the X-axis slide rail and the Y-axis slide rail, the resin tank can move along the X-axis direction and the Y-axis direction, and the exposure of different positions of the same resin liquid level can be realized, so that the splicing of a plurality of images on one plane is completed to form a larger layer.

The input of the mask plate pattern, the monitoring of the CCD camera, the electromagnetic auxiliary vibration device and the resin tank are driven by the bottom plate to move up and down on the Z1 shaft slide rail, the forming platform moves up and down on the Z2 shaft slide rail, and the moving platform moves on the Y shaft slide rail and the X shaft slide rail along the horizontal straight line in the Y shaft and X shaft directions, which are controlled by the computer PC.

After the preparation of the whole formed part is completed, the formed part is soaked in acetone solution, and micro corrosion is carried out on the formed part to expose the nano particles on the surface of the formed part, so that the micron/nano composite structure array with the antibacterial adhesion performance is formed.

Compared with the prior art, the invention has the beneficial effects that: (1) nano particles are distributed on the surface of the echinocandin structure to form a micron/nano composite structure; a plurality of micron/nanometer composite structures are uniformly distributed to form a micro-structure array; the surface microstructure array has good antibacterial adhesion, adsorption resistance and self-cleaning performance, and the microstructure appearance and size are controllable; (2) the electromagnetic auxiliary vibration device generates high-frequency vibration, and utilizes the impact waves generated by the cavitation action of the electromagnetic auxiliary vibration device and the crushing action of the micro jet to achieve the purposes of dispersing nano particles, reducing the agglomeration phenomenon and realizing the uniform distribution of the nano particles, thereby improving the forming precision and the forming quality; (3) the lower part of the moving platform is provided with a Y-axis sliding rail and an X-axis sliding rail which can horizontally move in a linear mode along the directions of the Y axis and the X axis, so that the splicing of a plurality of images on a plane is realized, the area of a forming layer surface and the size of a formed part and a micro-structure array can be enlarged, the forming time is shortened, and the forming efficiency is improved.

Drawings

Fig. 1 is a schematic overall structure diagram of a surface projection micro-stereolithography system with electromagnetic auxiliary vibration according to an embodiment of the present invention, fig. 2 is a schematic structural diagram of an electromagnetic auxiliary vibration device according to an embodiment of the present invention, fig. 3 is a schematic structural diagram of a target surface microstructure array of a molded part according to an embodiment of the present invention, and fig. 4 is a schematic structural diagram of a single echinocandin pavilion according to an embodiment of the present invention.

The labels in the above fig. 1 to 4 are: 1. the system comprises a marble base, 2 and Y-axis slide rails, 3, a moving platform, 4, a bottom plate, 5, Z1 axis slide rails, 6, a resin groove, 7, photosensitive resin, 8, a formed part, 9, an objective lens, 10 and 45-degree reflectors, 11, a CCD camera, 12, a light beam, 13, a mask plate, 14, a light machine, 15, a forming platform, 16 and Z2 axis slide rails, 17, nano particles, 18, an electromagnetic auxiliary vibration device, 19, a computer PC, 20 and X axis slide rails, 21, a magnetism isolating sleeve, 22, an armature, 23, a plate spring, 24, an iron core, 25, a coil, 26, a formed part target surface microstructure array, 27 and a formed part target surface; 28. a single sea urchin kiosk structure.

Detailed Description

Referring to the attached drawings 1-4, the single microstructure of the surface microstructure array with the antibacterial adhesion performance is a sea urchin pavilion structure 28, the material is photosensitive resin 7, and the transverse dimension, the longitudinal dimension and the spacing of the photosensitive resin are micron-sized; the echinocandin structure 28 is in a regular ellipsoid shape, the maximum diameter is 10 mu m, and the distance is 1 mu m; 8 reinforcing ribs are uniformly distributed along the ellipsoidal surface in an annular mode, the width of each reinforcing rib is 1 mu m, and the angle between every two reinforcing ribs is 45 degrees; on the surface of the microstructure, nanoparticles 17 are distributed, so as to form a micron/nanometer composite structure with the echinocandin structure 28; a plurality of the above-mentioned composite structures are uniformly distributed on the target surface 27 of the formed part, i.e. the surface microstructure array 26 is formed.

The method for forming the surface microstructure array 26 with the antibacterial adhesion property utilizes a set of electromagnetic auxiliary vibration surface projection micro-stereolithography system and completes the preparation by following certain steps.

The electromagnetic auxiliary vibration surface projection micro stereolithography system is provided with a marble base 1. The marble base 1 is provided with a Y-axis slide rail 2, an X-axis slide rail 20 and a mobile platform 3 from bottom to top respectively. The moving platform 3 can move horizontally and linearly on the Y-axis slide rail 2 and the X-axis slide rail 20 along the Y-axis and X-axis directions, respectively, and the Y-axis and X-axis directions are driven by linear motors, respectively.

Two sides of the moving platform 3 are respectively provided with a Z1 shaft slide rail 5 and a Z2 shaft slide rail 16.

And a bottom plate 4 is arranged on the Z1 shaft slide rail 5. The bottom plate 4 can move up and down along the Z1 axis direction on a Z1 axis slide rail 5, and the Z1 axis is driven by a linear motor. The bottom plate 4 is provided with an electromagnetic auxiliary vibration device 18 and a resin tank 6 from bottom to top. The electromagnetic auxiliary vibration device 18 comprises a magnetic isolation sleeve 21, an armature 22, a plate spring 23, an iron core 24 and a coil 25. The resin tank 6 contains photosensitive resin 7, and 10% of nanoparticles 17 are doped in the photosensitive resin 7.

And a forming platform 15 is arranged on the Z2 shaft slide rail 16. The forming platform 15 can move up and down along the Z2 axis direction on a Z2 axis slide rail 16, and the Z2 axis is driven by a linear motor. And a formed part 8 obtained by carrying out surface projection exposure and curing on photosensitive resin is supported on the forming platform 15.

An optical machine 14, a mask plate 13, a CCD camera 11, a 45-degree reflector 10 and an objective lens 9 are respectively arranged on the upper portion of the resin groove 6 from right to left.

And a computer PC19 is arranged on the right side of the resin tank 6.

To achieve the formation of the surface microstructure array 16 with anti-bacterial adhesion properties, the present invention follows the steps of:

the three-dimensional CAD model of the formed part 8 with the surface microstructure array 26 is sliced according to a certain thickness by using layering software, each layer of slices is converted into a bitmap file, and each bitmap file is input to the mask plate 13. The light beam 12 emitted from the optical machine 14 is shaped by the pattern of the mask plate 13 and contains the image information of the layer surface to be cured, and then is reflected by the 45-degree reflecting mirror 10 and focused by the objective lens 9 to be exposed on the liquid surface of the photosensitive resin 7 in the resin tank 6, so that the cured layer surface with the required pattern is obtained. After one layer is cured, the cured layer is sunk into the photosensitive resin 7 along with the downward movement of the forming platform 15, and the thickness of the photosensitive resin 7 covered on the cured layer is ensured to be exactly equal to the thickness of the next layer to be processed. Repeating the above exposure process until all layers are exposed. The CCD camera 11 is used for monitoring the forming and curing process of each layer.

In order to reduce the agglomeration phenomenon of the nano particles 17 in the molding process, the lower part of the resin tank 6 is provided with the electromagnetic auxiliary vibration device 18. The coil 25 is electrified with half-wave rectified pulse current, the current magnetizes the iron core 24 during positive half-wave to form an electromagnet and attract the armature 22, thereby driving the resin tank 6 to move downwards; the current disappears during the negative half wave and the resin tank 6 returns to its original position under the action of the leaf spring 23. This circulation realizes the vertical vibration of the resin tank 6. The magnetic isolation sleeve 21 is disposed outside the electromagnetic auxiliary vibration device 18 to prevent electromagnetic radiation from interfering with the outside.

The lower part of the moving platform 3 is provided with the X-axis slide rail 20 and the Y-axis slide rail 2, the resin tank 6 can move along the X-axis direction and the Y-axis direction, and the exposure of different positions of the same resin liquid level can be realized, so that the splicing of a plurality of images on one plane is completed to form a larger layer.

The input of the pattern of the mask plate 13, the monitoring of the CCD camera 11, the electromagnetic auxiliary vibration device 18 and the resin tank 6 are driven by the bottom plate 4 to move up and down on the Z1 shaft slide rail 5, the forming platform 15 moves up and down on the Z2 shaft slide rail 16, and the moving platform 3 moves on the Y shaft slide rail 2 and the X shaft slide rail 20 along the horizontal linear motion of the Y shaft and the X shaft, which are all controlled by the computer PC 19.

After the preparation of the whole formed part 8 is completed, the formed part 8 is soaked in acetone solution, and micro-corrosion is carried out on the formed part to expose the nano particles 17 on the surface of the formed part, so that the micron/nano composite structure array 26 with antibacterial adhesion performance is formed.

The foregoing detailed description is to be understood as being given by way of illustration only, and not as limitation of the scope of the invention, as various equivalent modifications of the invention will become apparent to those skilled in the art upon reading the present disclosure, as defined in the appended claims.