阅读说明：本技术 一种皮肤屏障修护复合物、肌底液及其制备方法 (Skin barrier repair compound, skin base fluid and preparation method thereof ) 是由 温伟红 徐洁琼 容惠 程文曲 于 2020-04-02 设计创作，主要内容包括：本发明提供一种皮肤屏障修护复合物、肌底液及其制备方法。所述皮肤屏障修护复合物包括：修复皮肤屏障的组合物、羟基积雪草甙、葡萄叶提取物、皱波角叉菜提取物、糖鞘脂类和糖脂；所述修复皮肤屏障的组合物包括：海茴香愈伤组织培养物滤液、四氢甲基嘧啶羧酸和水解红藻提取物。所述肌底液按质量百分含量计包括：1-10％所述的皮肤屏障修护复合物、10-30％保湿剂、1-10％润肤剂和1-5％乳化剂,余量为水。所述皮肤屏障修护复合物可有效平衡微生物屏障、稳定角质屏障、强化免疫力屏障,使肌肤健康稳定。由此皮肤屏障修护复合物制备得到的肌底液能够有效调节角质更新,强化肌肤角质屏障,提升肌肤抵御力。(The invention provides a skin barrier repair compound, a muscle base fluid and a preparation method thereof. The skin barrier repair composite comprises: a composition for repairing skin barrier, madecassoside, a grape leaf extract, a Chondrus crispus extract, glycosphingolipids and glycolipids; the composition for repairing skin barrier comprises: hypecoum vulgare callus culture filtrate, tetrahydro-methyl pyrimidine carboxylic acid and hydrolyzed red algae extract. The muscle base solution comprises the following components in percentage by mass: 1-10% of the skin barrier repair compound, 10-30% of a humectant, 1-10% of an emollient, 1-5% of an emulsifier, and the balance water. The skin barrier repair compound can effectively balance a microbial barrier, stabilize a cutin barrier, strengthen an immunity barrier and enable skin to be healthy and stable. The skin foundation liquid prepared from the skin barrier repair compound can effectively regulate cutin renewal, strengthen the skin cutin barrier and improve the skin resistance.)

1. A skin barrier repair composite, wherein the skin barrier repair composite comprises: a composition for repairing skin barrier, madecassoside, a grape leaf extract, a Chondrus crispus extract, glycosphingolipids and glycolipids;

the composition for repairing skin barrier comprises: hypecoum vulgare callus culture filtrate, tetrahydro-methyl pyrimidine carboxylic acid and hydrolyzed red algae extract.

2. The skin barrier repair composite of claim 1, comprising in parts by weight: 1-10 parts of composition for repairing skin barrier, 0.1-5 parts of madecassoside, 0.1-5 parts of grape leaf extract, 0.1-5 parts of Chondrus crispus extract, 0.1-1 part of glycosphingolipid and 0.1-1 part of glycolipid.

3. The skin barrier repairing complex according to claim 1 or 2, wherein the mass ratio of the hypsizygus marmoreus callus culture filtrate, the tetrahydromethylpyrimidine carboxylic acid and the hydrolyzed red algae extract is (3-9): (2-5): (1-3).

4. A muscle base fluid, comprising the skin barrier repair complex of any one of claims 1-3.

5. The muscle fluid base according to claim 4, wherein the muscle fluid base comprises, by mass:

1-10% of a skin barrier repair complex according to any one of claims 1-3, 10-30% of a moisturizer, 1-10% of an emollient, and 1-5% of an emulsifier, the balance being water.

6. The muscle base fluid of claim 5, wherein the moisturizer comprises any one of or a combination of at least two of glycerol, silanetriol, hyaluronic acid, sodium hyaluronate, xylitol, anhydroxylitol, xylitol, 1, 3-propanediol, 1, 2-hexanediol, butanediol, glyceryl polyether-26, sodium PCA, erythritol, or polyethylene glycol-32;

preferably, the emollient comprises any one or combination of at least two of dimethicone, dimethiconol, jojoba oil, bis-diglycerol polyacyladipate-2, grapefruit oil, geranium oil, coconut oil, gardenia extract, tocopherol, sunflower seed oil, or neroli oil;

preferably, the emulsifier comprises a C20-22 alcohol and/or a C20-22 alcohol phosphate.

7. The muscle base according to claim 5 or 6, further comprising 0.1-1% of an antioxidant;

preferably, the antioxidant is p-hydroxyacetophenone;

preferably, the muscle base fluid further comprises 0.5-3% of a thickening agent;

preferably, the thickener comprises any one of or a combination of at least two of polyacrylate cross-linked polymer-6, gum arabic, xanthan gum or hydroxyethyl acrylate/sodium acryloyldimethyl taurate copolymer;

preferably, the muscle base fluid further comprises 0.1-0.5% of a neutralizing agent;

preferably, the neutralizing agent is arginine.

8. A method of preparing a muscle base according to any one of claims 5 to 7, comprising the steps of:

(1) mixing the humectant and water to obtain a water-phase mixed solution; mixing an emollient and an emulsifier in an oil phase to obtain an oil phase mixed solution;

(2) mixing the water phase mixed liquor and the oil phase mixed liquor obtained in the step (1) and homogenizing to obtain homogenized liquor;

(3) mixing and stirring the homogenized liquid obtained in the step (2) and the skin barrier repair compound of any one of claims 1-3 to obtain the muscle base liquid.

9. The method of manufacturing according to claim 8, comprising the steps of:

(1) mixing the humectant, the thickener and water in a water phase to obtain a water phase mixed solution; mixing an emollient and an emulsifier in an oil phase to obtain an oil phase mixed solution;

(2) mixing the water phase mixed liquor and the oil phase mixed liquor obtained in the step (1) and homogenizing to obtain homogenized liquor;

(3) adding the skin barrier repair compound according to any one of claims 1-3, an antioxidant and a neutralizing agent to the homogenized solution of step (2), and mixing and stirring to obtain the muscle base solution;

preferably, the temperature of the water phase mixing in the step (1) is 80-85 ℃;

preferably, the time for mixing the water phase in the step (1) is 5-10 min;

preferably, the temperature of the oil phase mixing in the step (1) is 78-80 ℃;

preferably, the time for mixing the oil phase in the step (1) is 5-10 min;

preferably, the temperature for mixing and homogenizing in the step (2) is 80-82 ℃;

preferably, the mixing and homogenizing in the step (2) are carried out for 5-10 min;

preferably, the temperature of the mixing and stirring in the step (3) is 40-50 ℃;

preferably, the mixing and stirring time in the step (3) is 5-10 min.

10. The method of preparing a muscle base fluid according to claim 8 or 9, wherein the method comprises the steps of:

(1) stirring and mixing the humectant, the thickener and water at 80-85 deg.C for 5-10min to obtain water phase mixed solution; mixing emollient and emulsifier at 78-80 deg.C under stirring for 5-10min to obtain oil phase mixed solution;

(2) mixing the water phase mixed liquor and the oil phase mixed liquor obtained in the step (1) at 80-82 ℃ and homogenizing for 5-10min to obtain homogenized liquor;

(3) adding the skin barrier repair compound according to any one of claims 1 to 3, an antioxidant and a neutralizing agent to the homogenized solution of step (2), and mixing and stirring at 40-50 ℃ for 5-10min to obtain the muscle base solution.

Technical Field

The invention belongs to the technical field of cosmetics, and particularly relates to a skin barrier repair compound, a skin barrier repair liquid and a preparation method of the skin barrier repair compound.

Background

The skin is a natural barrier of the human body surface, a plurality of microorganisms exist, the skin micro-ecological balance keeps the stability of the skin environment, and the skin has important effect on the health of the body. Healthy skin can effectively retain moisture, thereby maintaining good elastic and shiny appearance of the skin. However, the skin is affected by the living environment and the self-physiological conditions, and skin problems such as sensitivity, dry desquamation, excessive grease secretion, color spots, skin relaxation and aging, red blood streak and the like can occur on the skin, and the most important reason is that the skin barrier is damaged. The skin barrier is a protective structure which is constructed by the cuticle and the sebum membrane together, can buffer physical and chemical stimulation, effectively inhibit bacterial breeding, block the harm of bacteria, viruses and fungi to the skin, lock moisture and prevent the moisture from evaporating, thereby realizing the function of keeping moisture. Skin barrier damage causes skin to be attacked by bacteria, viruses and fungi, and causes unscrupulous evaporation of moisture in the skin, thereby causing various problems in the skin.

The skin care products for repairing skin barriers commonly used at present have some disadvantages: firstly, only the enhancement of the efficacy is focused, but the health of the skin barrier is neglected, only healthy skin can better absorb nutrition, only healthy skin can be far away from various problems, and the youth and beauty can be kept for a long time. Secondly, only the single barrier is paid attention to the maintenance, but other barriers of the skin are ignored, the surface of the skin looks good, and the problem of deep skin is difficult to solve.

CN102349867A discloses a moisturizing repair cosmetic and a preparation method and application thereof. The cosmetic comprises a moisturizing repair freeze-dried powder and a moisturizing repair basic liquid. The main components of the freeze-dried powder comprise grape seed extract, hydrolyzed red algae extract, hyaluronic acid with small molecular weight, hydrolyzed collagen, recombinant human keratinocyte growth factor, recombinant human epidermal cell growth factor and low molecular weight heparin sodium. The moisturizing and repairing cosmetic can be used for daily skin care and has the beautifying effects of moisturizing skin, repairing water-deficient damaged cells and enhancing skin elasticity.

CN109316439A discloses a suspension repair essence containing aniseed stem cells, which comprises the following components in percentage by mass: 0.01-0.5% of sea fennel stem cells, 1-10% of glycerol, 0.1-10% of sorbitol, 0.1-5% of agar, 0.01-2% of hyaluronate and 0.05-2% of poloxamer. The suspension repair essence disclosed by the invention has the effects of synergistically repairing, moisturizing and enhancing the barrier of the dermis. .

Therefore, the development of a skin care product capable of balancing the skin microbial barrier, stabilizing the cutin barrier and strengthening the immunity barrier is the focus of the current research.

Disclosure of Invention

The invention aims to provide a skin barrier repair compound, a muscle base fluid and a preparation method thereof. The skin barrier repair compound can effectively repair a microorganism barrier, a cutin barrier and an immune barrier, so that the skin is healthy and stable. The skin base fluid prepared from the skin barrier repair compound can effectively regulate the keratin renewal, strengthen the skin keratin barrier, improve the skin resistance, effectively balance the microbial barrier, stabilize the keratin barrier and strengthen the immunity barrier, so that the skin can maintain healthy metabolism and achieve ideal moist, glossy and healthy states.

In order to achieve the purpose, the invention adopts the following technical scheme:

in a first aspect, the present invention provides a skin barrier repair composition comprising: a composition for repairing skin barrier, madecassoside, a grape leaf extract, an extract of coleus forskohlii, glycosphingolipids and glycolipids;

the composition for repairing skin barrier comprises: hypecoum vulgare callus culture filtrate, tetrahydro-methyl pyrimidine carboxylic acid and hydrolyzed red algae extract.

In the composition for repairing the skin barrier, the hypsizygus marmoreus callus culture filtrate, the tetrahydromethylpyrimidine carboxylic acid and the hydrolyzed red algae extract are matched with each other and synergized, so that the skin barrier is comprehensively repaired. By strengthening the microbial barrier, the physicochemical barrier and the immune barrier of the skin, harmful invasion factors are isolated outside the skin, and a complete and healthy skin defense line is constructed, so that various skin problems are fundamentally solved. In addition, the composition has anti-inflammatory and soothing effects, and can quickly and effectively soothe inflammatory reaction and discomfort symptoms of the skin, and maintain the steady state and basic health of the skin.

The hypsizygus marmoreus callus culture filtrate is prepared by selecting a rare saline soil plant hypsizygus marmoreus, and culturing stem cell active factors through a special process, so that the regeneration of cutin is effectively regulated, the cutin barrier of skin is strengthened, and the skin resistance is improved. The tetrahydromethylpyrimidine carboxylic acid is extracted from halophilic bacteria vitamin factors in desert salt lakes, can effectively protect skin immune barriers, enables the skin to be free from external damage, and keeps youth for a long time. The hydrolyzed red algae extract is preferably Durvillea in pure sea area, effectively improves the micro-ecological environment of skin, enables the number of resident flora to reach a beneficial balanced state, enables the skin to maintain healthy metabolism, and achieves ideal moisture, luster and healthy states.

In the invention, the madecassoside can promote the growth of fibroblasts, and the fibroblasts play an important role in the process of repairing skin injury, for example, in the process of wound healing, the fibroblasts are massively proliferated through mitosis, synthesize and secrete a large amount of collagen fibers and matrix components, and form granulation tissues together with new capillaries and the like, so that the defect of the wound tissues is filled, conditions are created for covering epidermal cells, and the skin injury is repaired; promoting collagen synthesis and epithelial cell growth; can inhibit inflammation to achieve anti-inflammatory effect.

In the present invention, the grape leaf extract prolongs the life of cells and also enhances the activity of the skin; has the functions of eliminating free radicals, inhibiting collagenase and nitrogen oxide generation, and shows the capability of inhibiting aging in many aspects; has proliferation effect on melanocyte, and can be used for hair conditioner product to reduce grey hair generation; has effects of shrinking and astringing pore diameter, and tightening skin.

In the present invention, the Chondrus crispus extract inhibits human leukocyte elastase and exhibits anti-inflammatory activity; has effects in promoting collagen production and activating.

In the invention, the glycosphingolipids are effective in improving the adhesive force of keratinocytes, enabling the cells to be tightly bound and reducing water loss; glycolipids can quickly penetrate into the skin and combine with water in the stratum corneum to form a network structure, which holds water.

According to the invention, all components in the skin barrier repairing compound are matched with each other, so that the synergistic effect is realized, the skin barrier is strengthened in a three-dimensional manner, and the skin barrier comprises a microorganism barrier, a cutin barrier and an immunity barrier, so that the skin is healthy and stable, and the youthful vigor is refreshed from inside to outside.

Preferably, the skin barrier repair compound comprises, in parts by weight: 1-10 parts of composition for repairing skin barrier, 0.1-5 parts of madecassoside, 0.1-5 parts of grape leaf extract, 0.1-5 parts of Chondrus crispus extract, 0.1-1 part of glycosphingolipid and 0.1-1 part of glycolipid.

In the skin barrier repair compound, the weight part of the composition for repairing the skin barrier is 1-10 parts, and may be, for example, 1 part, 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts, etc.

In the skin barrier repair compound, the parts by weight of the madecassoside are 0.1-5 parts, and may be, for example, 0.1 part, 0.5 part, 1 part, 1.5 parts, 2 parts, 3 parts, 4 parts, 5 parts, etc.

In the skin barrier repair composition, the weight part of the grape leaf extract is 0.1 to 5 parts, and may be, for example, 0.1 part, 0.5 part, 1 part, 1.5 parts, 2 parts, 3 parts, 4 parts, 5 parts, and the like.

In the skin barrier repair composition, the weight part of the carrageen crispus extract is 0.1-5 parts, for example, 0.1 part, 0.5 part, 1 part, 1.5 parts, 2 parts, 3 parts, 4 parts, 5 parts and the like.

The skin barrier repair complex may contain glycosphingolipids in an amount of 0.1 to 1 part by weight, and examples thereof include 0.1 part, 0.2 part, 0.3 part, 0.4 part, 0.5 part, 0.6 part, 0.7 part, 0.8 part, 0.9 part, and 1 part.

In the skin barrier repair complex, the glycolipid may be 0.1-1 part by weight, for example, 0.1 part, 0.2 part, 0.3 part, 0.4 part, 0.5 part, 0.6 part, 0.7 part, 0.8 part, 0.9 part, 1 part, etc.

Preferably, the mass ratio of the hypsizygus marmoreus callus culture filtrate, the tetrahydromethylpyrimidine carboxylic acid and the hydrolyzed red algae extract is (3-9): (2-5): (1-3).

Wherein, 3-9 can be 3, 3.5, 4, 4.5, 5, 5.5, 6, 6.5, 7, 7.5, 8, 8.5, 9, etc.; wherein, 2-5 can be 2, 2.2, 2.5, 2.8, 3, 3.2, 3.5, 3.8, 4, 4.2, 4.5, 4.8, 5, etc.; wherein "1 to 3" may be 1, 1.2, 1.4, 1.5, 1.6, 1.8, 2, 2.2, 2.4, 2.6, 2.8, 3, etc.

In a second aspect, the present invention provides a muscle base fluid comprising a skin barrier repair complex according to the first aspect.

Preferably, the muscle base fluid comprises the following components in percentage by mass: 1-10% of the skin barrier repair complex of the first aspect, 10-30% of a humectant, 1-10% of an emollient, 1-5% of an emulsifier, and the balance water.

The content of the skin barrier repair complex is 1 to 10%, for example, 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, etc., based on 100% by mass of the total muscle base fluid.

The content of the humectant is 10 to 30%, for example, 10%, 12%, 14%, 16%, 18%, 20%, 22%, 24%, 26%, 28%, 30%, etc., based on 100% by mass of the total muscle foundation solution.

The content of the emollient is 1 to 10%, for example, 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, etc., based on 100% by mass of the total muscle foundation solution.

The content of the emulsifier is 1 to 5%, for example, 1%, 2%, 3%, 4%, 5% or the like, based on 100% by mass of the total muscle fluid.

Preferably, the moisturizer comprises any one of glycerin, silanetriol, hyaluronic acid, sodium hyaluronate, xylitol, anhydroxylitol, xylitol-based glucoside, 1, 3-propanediol, 1, 2-hexanediol, butanediol, glycerol polyether-26, sodium PCA, erythritol, or polyethylene glycol-32, or a combination of at least two thereof.

Preferably, the emollient comprises any one or a combination of at least two of dimethicone, dimethiconol, jojoba seed oil, bis-diglycerol polyacyladipate-2, grapefruit oil, geranium oil, coconut oil, gardenia extract, tocopherol, sunflower seed oil, or neroli oil.

Preferably, the emulsifier comprises a C20-22 alcohol and/or a C20-22 alcohol phosphate.

Optionally, the muscle base fluid further comprises 0.1-1% of an antioxidant, which may be, for example, 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, etc.

Preferably, the antioxidant is p-hydroxyacetophenone.

Preferably, the muscle base fluid also includes 0.5-3% of a thickening agent, which may be, for example, 0.5%, 0.6%, 0.8%, 1%, 1.2%, 1.4%, 1.6%, 1.8%, 2%, 2.2%, 2.4%, 2.6%, 2.8%, 3%, etc.

Preferably, the thickener comprises any one of polyacrylate crosspolymer-6, gum arabic, xanthan gum, or hydroxyethyl acrylate/sodium acryloyldimethyl taurate copolymer, or a combination of at least two thereof.

Preferably, the muscle base fluid further comprises 0.1-0.5% of a neutralizing agent, which may be, for example, 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, etc.

Preferably, the neutralizing agent is arginine.

In a third aspect, the present invention provides a method for preparing a muscle base fluid as described in the second aspect, the method comprising the steps of:

(1) mixing the humectant and water to obtain a water-phase mixed solution; mixing an emollient and an emulsifier in an oil phase to obtain an oil phase mixed solution;

(2) mixing the water phase mixed liquor and the oil phase mixed liquor obtained in the step (1) and homogenizing to obtain homogenized liquor;

(3) and (3) mixing and stirring the homogenized solution obtained in the step (2) and the skin barrier repair compound obtained in the first aspect to obtain the muscle base solution.

Preferably, the preparation method comprises the following steps:

(1) mixing the humectant, the thickener and water in a water phase to obtain a water phase mixed solution; mixing an emollient and an emulsifier in an oil phase to obtain an oil phase mixed solution;

(2) mixing the water phase mixed liquor and the oil phase mixed liquor obtained in the step (1) and homogenizing to obtain homogenized liquor;

(3) adding the skin barrier repair compound of the first aspect, an antioxidant and a neutralizing agent into the homogenized liquid obtained in the step (2), and mixing and stirring to obtain the muscle base liquid.

Preferably, the temperature of the aqueous phase mixing in step (1) is 80 to 85 ℃, for example, 80 ℃, 81 ℃, 82 ℃, 83 ℃, 84 ℃, 85 ℃ and the like.

Preferably, the time for mixing the water phase in step (1) is 5-10min, such as 5min, 6min, 7min, 8min, 9min, 10min, etc.

Preferably, the temperature at which the oil phase is mixed in step (1) is 78 to 80 ℃, and may be, for example, 78 ℃, 78.5 ℃, 79 ℃, 79.5 ℃, 80 ℃ or the like.

Preferably, the time for mixing the oil phase in step (1) is 5-10min, such as 5min, 6min, 7min, 8min, 9min, 10min, etc.

Preferably, the temperature for mixing and homogenizing in the step (2) is 80-82 deg.C, such as 80 deg.C, 80.5 deg.C, 81 deg.C, 81.5 deg.C, 82 deg.C, etc.

Preferably, the mixing and homogenizing time in the step (2) is 5-10min, such as 5min, 6min, 7min, 8min, 9min, 10min and the like.

Preferably, the mixing and stirring temperature in step (3) is 40-50 deg.C, such as 40 deg.C, 42 deg.C, 44 deg.C, 46 deg.C, 48 deg.C, 50 deg.C, etc.

Preferably, the mixing and stirring time in step (3) is 5-10min, such as 5min, 6min, 7min, 8min, 9min, 10min, etc.

Preferably, the preparation method of the muscle base fluid comprises the following steps:

(1) mixing humectant, thickener and water at 80-85 deg.C under stirring for 5-10min to obtain water phase mixed solution; mixing emollient and emulsifier at 78-80 deg.C under stirring for 5-10min to obtain oil phase mixed solution;

(2) mixing the water phase mixed solution and the oil phase mixed solution obtained in the step (1) at 80-82 ℃ and homogenizing for 5-10min to obtain a homogenized solution

(3) Adding the skin barrier repair compound, the antioxidant and the neutralizing agent into the homogenized liquid obtained in the step (2), and mixing and stirring at 40-50 ℃ for 5-10min to obtain the muscle base liquid.

Compared with the prior art, the invention has the following beneficial effects:

(1) the skin barrier repair compound disclosed by the invention is prepared by three-dimensionally strengthening a skin barrier: microbial barrier, keratinous barrier, immune barrier, thus stabilizing skin health. By strengthening the microbial barrier, the physicochemical barrier and the immune barrier of the skin, harmful invasion factors are isolated outside the skin, and a complete and healthy skin defense line is constructed, so that various skin problems are fundamentally solved.

(2) The skin base fluid prepared from the skin barrier repair compound can effectively regulate the regeneration of cutin, strengthen the skin cutin barrier, improve the skin resistance, effectively improve the skin micro-ecological environment, enable the number of resident flora to reach a beneficial balanced state, enable the skin to maintain healthy metabolism and achieve ideal moist, glossy and healthy states.

Detailed Description

The technical solution of the present invention is further explained by the following embodiments. It should be understood by those skilled in the art that the examples are only for the understanding of the present invention and should not be construed as the specific limitations of the present invention.

The compositions for repairing skin barriers provided in the following examples were prepared by the following methods:

preparation example 1

The preparation method for the hypecoum vulgare callus culture filtrate comprises the following steps:

(1) callus induction culture: inoculating the hypecoum erectum explant into an agar culture medium for callus induction culture, wherein the temperature of the induction culture is 20 ℃, and the time of the induction culture is 14 days, so as to obtain callus;

(2) suspension culture of callus particles: inoculating the callus obtained in the step (1) into an MS culture medium, and performing shake culture in a shake culture box at a rotating speed of 100r/min, wherein the temperature of the shake culture is 20 ℃, and the time of the shake culture is 14 days, so as to obtain a proliferation callus;

(3) expanding and culturing the callus tissue: inoculating the proliferated callus obtained in the step (2) into a sterile cell bag for amplification culture at the temperature of 25 ℃ for 24 days to obtain the hypecoum erectum callus culture;

(4) separation: and (4) filtering the hypecoum vulgare callus culture obtained in the step (3) through gauze of 100 meshes, and then performing vacuum drying at 25 ℃ to obtain the fennel callus culture filtrate.

Preparation example 2

The preparation example prepares the tetrahydro-methyl pyrimidine carboxylic acid, and the preparation method of the tetrahydro-methyl pyrimidine carboxylic acid comprises the following steps:

(A) culturing desert halophilic bacteria: inoculating desert halophilic bacteria into an SCDLP liquid culture medium, and culturing in a shaking incubator at the rotating speed of 1000r/min, wherein the culturing temperature is 20 ℃, and the culturing time is 48h, so as to obtain desert halophilic bacteria thallus;

(B) osmotic shock: resuspending the desert halophilic bacteria thallus obtained in the step (A) in a high osmotic pressure solution (the high osmotic pressure solution comprises 18% of sucrose, 0.1% of EDTA and the balance of water by mass percent), soaking for 1h, and filtering to obtain a precipitate desert halophilic bacteria thallus;

(C) electrodialysis: and (C) carrying out electrodialysis treatment on the desert halophilic bacteria thallus obtained in the step (B) to obtain the tetrahydro-methyl pyrimidine carboxylic acid.

Preparation example 3

The preparation example prepares a hydrolyzed red algae extract, and the preparation method of the hydrolyzed red algae extract comprises the following steps:

(a) extraction: cleaning Duerweili algae, pulverizing, and sieving with 100-fold 200-mesh sieve to obtain red algae powder; adding water into red algae powder, extracting at 70 ℃ for the first time, wherein the first extraction time is 10 hours, the mass ratio of the water to the red algae powder is 20:1, obtaining a first extracting solution, filtering the first extracting solution, concentrating, adding an ethanol water solution with the volume ratio concentration of 80%, extracting for the second time, standing, and taking a precipitate to obtain a red algae polysaccharide crude material;

(b) hydrolysis: dissolving the red algae polysaccharide crude material obtained in the step (a) in water, adding 30 wt% of aqueous hydrogen peroxide, and mixing and stirring at 50 ℃ for 1h to obtain hydrolyzed red algae extracting solution;

(c) and (3) ultrafiltration: and (c) treating the hydrolyzed red algae extracting solution obtained in the step (b) by using an ultrafiltration membrane, concentrating and desalting, wherein the ultrafiltration time is 30min, and the ultrafiltration temperature is 30 ℃ to obtain a hydrolyzed red algae extract.

Preparation example 4

The preparation example provides a composition for repairing skin barrier, and the preparation method of the composition for repairing skin barrier comprises the following steps: the composition for repairing skin barrier was obtained by mixing 7 parts of the hypsizygus marmoreus callus culture filtrate of preparation example 1,3 parts of the tetrahydro-methylpyrimidine carboxylic acid of preparation example 2, and 2 parts of the hydrolyzed red algae extract of preparation example 3 at 20 ℃ for 20 min.