阅读说明：本技术 一种基于柑桔幼果提取物的蛋鸡饲料添加剂及其应用 (Laying hen feed additive based on citrus immature fruit extract and application thereof ) 是由 谭时勋 黄友生 于 2020-06-29 设计创作，主要内容包括：本发明涉及一种基于柑桔幼果提取物的蛋鸡饲料添加剂及其应用。该蛋鸡饲料添加剂,按照质量百分比,包括：柑桔幼果提取物80～90％,枫香树叶提取物5～10％,女贞子提取物5～10％；其中,所述柑桔幼果提取物中,总黄酮含量为10％以上,总多糖含量为10％以上；所述枫香树叶提取物中,总多酚5％以上；所述女贞子提取物中,齐墩果酸5％以上；所述柑桔幼果提取物由直径小于2厘米的柑桔提取得到。本发明通过鸡饲料添加剂各个组分的协同作用在确保蛋鸡健康喂养的前提下提高鸡蛋蛋黄的色度。(The invention relates to a laying hen feed additive based on citrus immature fruit extract and application thereof. The laying hen feed additive comprises the following components in percentage by mass: 80-90% of citrus immature fruit extract, 5-10% of liquidambar formosana leaf extract and 5-10% of glossy privet fruit extract; wherein, in the extract of the young citrus fruits, the content of total flavone is more than 10 percent, and the content of total polysaccharide is more than 10 percent; the total polyphenol content in the liquidambar formosana leaf extract is more than 5%; in the glossy privet fruit extract, oleanolic acid accounts for more than 5 percent; the citrus young fruit extract is extracted from citrus with a diameter of less than 2 cm. The invention improves the chroma of the egg yolk of the egg on the premise of ensuring the healthy feeding of the laying hens through the synergistic effect of all components of the chicken feed additive.)

1. A laying hen feed additive based on citrus immature fruit extract is characterized by comprising the following components in percentage by mass: 80-90% of citrus immature fruit extract, 5-10% of liquidambar formosana leaf extract and 5-10% of glossy privet fruit extract; wherein, in the extract of the young citrus fruits, the content of total flavone is more than 10 percent, and the content of total polysaccharide is more than 10 percent; the total polyphenol content in the liquidambar formosana leaf extract is more than 5%; in the glossy privet fruit extract, oleanolic acid accounts for more than 5 percent; the citrus young fruit extract is extracted from citrus with a diameter of less than 2 cm.

2. The chicken feed additive of claim 1, wherein the citrus immature fruit extract is prepared by the steps of:

drying and crushing young citrus fruits, mixing the dried and crushed young citrus fruits with 70-90% ethanol according to the mass ratio of 1: 6-10, performing reflux extraction for 2-4 hours, and then concentrating and drying to obtain a first extract and a first residue; the citrus young fruit is a citrus with the diameter of less than 2 cm;

mixing the first residue with 30-50% ethanol by volume according to a mass ratio of 1: 6-10, performing reflux extraction for 2-4 hours, and then concentrating and drying to obtain a second extract and a second residue;

mixing the second residue with water according to a mass ratio of 1: 6-10, performing reflux extraction for 2-4 hours, and then concentrating and drying to obtain a third extract;

combining the first extract, the second extract and the third extract to obtain the citrus immature fruit extract.

3. Use of the layer feed additive of any of claims 1-2 in a layer feed.

4. Use according to claim 3, wherein the feed is added to the layer feed in an amount of 10-100 g/ton feed.

Technical Field

The invention relates to the field of laying hen feed, in particular to a laying hen feed additive based on citrus immature fruit extract and application thereof.

Background

The quality of egg yolk includes the color, size, flavor and ingredient content of egg yolk. There are many factors that affect the quality of egg yolk.

The yolk colour is formed by fat-soluble pigments which are deposited into the yolk during the formation of the bird's eggs, the body itself having no ability to synthesize these pigments, the pigments in the yolk being obtained from the feed. The ideal yolk color depends on the quality and amount of lutein in the diet, and the appropriate amount of red pigment can improve the deposition efficiency of lutein in yolk.

The synthetic pigment commonly used in laying hen production comprises capsanthin, luteal leaf, cryptoxanthin, violaxanthin, zeaxanthin, etc. At present, the artificial synthetic pigment substances in the feed additive are clearly specified at home and abroad, and the addition amount of the artificial synthetic pigment substances in the feed is required to be not more than 30 g/t. Complete coloration of egg yolk generally takes 10 days, and in production, feed containing pigment is fed for two weeks before egg laying so as to completely generate the color of egg yolk.

The absorption process of lutein is similar to that of fat-soluble vitamins, and the lutein is combined with bile salt in small intestine to form mixed micelle, enters a near water layer on the surface of small intestine mucous membrane, a small part (or a few types) is re-esterified in the small intestine mucous membrane, and the majority enters blood and lymph in circulation in a diffusion mode, and is adsorbed by an active transportation mode and deposited in the skin.

The yellow pigment deposition is directly influenced by the health condition of the chicken body, and particularly, the yellow pigment deposition is influenced more obviously when the absorption function of the intestinal tract is failed (such as the form structure and the function of villus of small intestine are changed) or the immune function is reduced. Especially, the yellow pigment deposition can be reduced due to the infection of infectious diseases or the occurrence of parasite infection, especially acute and chronic coccidiosis, salmonella, chronic respiratory diseases, and the like. Infectious anemia, hemorrhagic enteritis, infectious bronchitis, colibacillosis, newcastle disease, viral arthritis, etc., all cause poor coloration. In addition, when the liver or pancreas is diseased, the bile secretion is reduced, the absorption of fat-soluble lutein can be greatly reduced, and it is reported that the level of lutein in the blood serum of the broiler chicken infected with coccidium is reduced by 88%, and the total amount of lutein in the intestinal tract is reduced by 76%.

Disclosure of Invention

The technical problem to be solved by the invention is as follows: the color of egg yolk is improved on the premise of ensuring the healthy feeding of laying hens through the synergistic effect of all components of the laying hen feed additive.

In order to solve the technical problems, the invention provides a laying hen feed additive based on a citrus immature fruit extract and application thereof.

A laying hen feed additive based on citrus immature fruit extract comprises the following components in percentage by mass: 80-90% of citrus immature fruit extract, 5-10% of liquidambar formosana leaf extract and 5-10% of glossy privet fruit extract; wherein, in the extract of the young citrus fruits, the content of total flavone is more than 10 percent, and the content of total polysaccharide is more than 10 percent; the total polyphenol content in the liquidambar formosana leaf extract is more than 5%; in the glossy privet fruit extract, oleanolic acid accounts for more than 5 percent; the citrus young fruit extract is extracted from citrus with a diameter of less than 2 cm.

Further, the citrus immature fruit extract is prepared by the following steps:

drying and crushing young citrus fruits, mixing the dried and crushed young citrus fruits with 70-90% ethanol according to the mass ratio of 1: 6-10, performing reflux extraction for 2-4 hours, and then concentrating and drying to obtain a first extract and a first residue; (ii) a The citrus young fruit is a citrus with the diameter of less than 2 cm;

mixing the first residue with 30-50% ethanol by volume according to a mass ratio of 1: 6-10, performing reflux extraction for 2-4 hours, and then concentrating and drying to obtain a second extract and a second residue;

mixing the second residue with water according to a mass ratio of 1: 6-10, performing reflux extraction for 2-4 hours, and then concentrating and drying to obtain a third extract;

combining the first extract, the second extract and the third extract to obtain the citrus immature fruit extract.

The invention also provides application of the laying hen feed additive in laying hen feed.

Further, the feed additive is added into the laying hen feed according to the addition amount of 10-100 g/ton feed.

The invention selects the citrus fruits with the diameter less than 2 cm as the extraction raw material, and is particularly suitable for animal feed because the young fruits have little pulp and the content of bitter substances such as limonin which affect the appetite of animals is extremely low compared with the mature fruits. The citrus immature fruit extract contains a plurality of vitamins, such as vitamin E, vitamin B2 and nicotinic acid; contains multiple major and trace elements, wherein calcium, potassium, iron, zinc, selenium and copper are contained in the major elements; 1.9 to 3.5 percent of faint scent volatile oil (containing more limonene, humulene and linoleic acid ethyl ester); contains natural pigment such as carotene, cryptoxanthin, citrinin, xanthophyll, etc.; contains various glycosides, wherein neohesperidin is higher in content; contains pectin dietary fiber and other nutrients. In addition, the young citrus fruits also contain various alkaloids, and a plurality of bioactive substances such as hesperidin, nobilene, choline, thymol, nerol, elemene, limonin and the like, the extraction and utilization of the active ingredients can reduce the occurrence of animal intestinal diseases and effectively improve the feeding performance of animals, and meanwhile, the active ingredients can effectively prevent the oxidative damage of peroxide and free radicals to pigments in vivo and in vitro.

Compared with the prior art, the invention has the advantages that: the extract of Citrus reticulata blanco has various active ingredients including natural pigment such as carotene, cryptoxanthin, citrinin, xanthophyll, etc., and the active ingredients include alkaloid, and hesperidin, nobilene, choline, thymol, nerol, elemene, and limonin; the active ingredients can fully ensure the antioxidation and nutrition supply effects of the citrus immature fruit extract on the laying hens; on the basis, the extract of the citrus immature fruit can prolong the existence time of the pigment in the egg yolk, and improve the chroma and the shelf life of the egg; the small amount of liquidambar formosana leaf extract containing total polyphenol in the laying hen feed additive can effectively improve the immunity of laying hens, promote the growth of intestinal probiotics, reduce the growth and reproduction of harmful bacteria, inhibit inflammation, improve the nutrient absorption of the laying hens and the high-efficiency conversion of feed nutrition; the glossy privet fruit extract in the laying hen feed additive can promote the growth and the immunoregulation of laying hens, thereby improving the feeding performance of the laying hens. Meanwhile, oleanolic acid of the glossy privet fruit extract can enhance the pigment transport of a transport system to an egg laying system by promoting blood circulation and improving the binding capacity of pigment and blood; therefore, the invention improves the chroma of the egg yolk under the premise of ensuring the healthy feeding of the laying hens by the synergistic effect of all components of the laying hen feed additive.

Detailed Description

The present invention will be described in detail with reference to the following embodiments in order to make the aforementioned objects, features and advantages of the invention more comprehensible. In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein, but rather should be construed as broadly as the present invention is capable of modification in various respects, all without departing from the spirit and scope of the present invention.

The specific embodiment provides a chicken feed additive which comprises the following components in percentage by mass: 80-90% of citrus immature fruit extract, 5-10% of liquidambar formosana leaf extract and 5-10% of glossy privet fruit extract; wherein, in the extract of the young citrus fruits, the content of total flavone is more than 10 percent, and the content of total polysaccharide is more than 10 percent; the total polyphenol content in the liquidambar formosana leaf extract is more than 5%; in the glossy privet fruit extract, oleanolic acid accounts for more than 5 percent;

the citrus immature fruit extract is prepared by the following steps:

collecting young citrus fruits, drying the young citrus fruits for 12-24 hours at 40-60 ℃ in a vacuum drying oven to obtain the required young citrus fruits, and crushing the dried young citrus fruits to obtain 10-40-mesh fine powder;

mixing the crushed young citrus fruits with 70-90% ethanol in a mass ratio of 1: 6-10 in an extraction tank, performing reflux extraction for 2-4 hours, and then concentrating and drying to obtain a first extract and a first residue; the citrus young fruit is a citrus with the diameter of less than 2 cm;

mixing the first residue and 30-50% ethanol by volume in an extraction tank according to the mass ratio of 1: 6-10, performing reflux extraction for 2-4 hours, and then concentrating and drying to obtain a second extract and a second residue;

mixing the second residue and water in an extraction tank according to the mass ratio of 1: 6-10, performing reflux extraction for 2-4 hours, and then concentrating and drying to obtain a third extract;

combining the first extract, the second extract and the third extract to obtain the citrus immature fruit extract.

The specific embodiment also comprises the application of the laying hen feed additive in laying hen feed, wherein the laying hen feed additive is added into the laying hen feed according to the addition amount of 10-100 g/ton feed.

The specific embodiment also comprises the application of the chicken feed additive in chicken feed, wherein the chicken feed additive is added into the chicken feed according to the addition amount of 10-100 g/ton feed; the chicken feed comprises, by mass, 60-65% of corn, 18-22% of soybean meal, 2-4% of cottonseed meal, 1-1.5% of calcium hydrophosphate, 8-10% of stone powder, 1.65-1.8% of bran, 0.3-0.4% of salt and 1-2% of premix.

The premix used in the invention is any commercially available premix suitable for chicken feed in the prior art. The liquidambar formosana leaf extract and the glossy privet fruit extract used in the invention are extracted by an ethanol solvent through a conventional extraction method, and can also be purchased from commercially available liquidambar formosana leaf extract and glossy privet fruit extract.

To further illustrate the layer feed additive and the application thereof provided by the embodiment, the following examples are given for further illustration.