阅读说明：本技术 纳米纤维毡布在制备骨缺损修复产品或促进成骨产品中的用途 (Application of nanofiber felt cloth in preparation of bone defect repair product or osteogenesis promoting product ) 是由 林开利 俞彬 乔之光 *** 沈国芳 连梅菲 韩煜 王玮琪 戴尅戎 于 2020-07-02 设计创作，主要内容包括：本发明提供纳米纤维毡布在制备骨缺损修复产品或促进成骨产品中的用途。本申请中的纳米纤维毡布可有效收集生物机械能并将其转化成电能,并直接用于骨缺损部位细胞的电刺激,在无需外接供能系统和复杂电路的同时,实现宿主的自驱动电刺激成骨。(The invention provides application of nanofiber felt cloth in preparing bone defect repair products or osteogenesis promoting products. The nano-fiber felt cloth can effectively collect the biological mechanical energy and convert the biological mechanical energy into electric energy, is directly used for electrical stimulation of cells at the bone defect part, and realizes self-driven electrical stimulation osteogenesis of a host while needing no external energy supply system and complex circuit.)

1. The application of the nanofiber felt cloth in preparing bone defect repair products or osteogenesis promoting products.

2. Use according to claim 1, characterized in that the nanofiber mat is a non-woven fabric of hollow nanofibers formed from a biodegradable polymer, the nanofibers in the nanofiber mat having an orientation.

3. Use according to claim 1, wherein the nanofiber felt has a grammage of (1-15) g/m²。

4. Use according to any one of claims 1 to 3, wherein the nanofiber felt is prepared by a method comprising: taking an organic solvent solution of a biodegradable polymer as a skin layer spinning solution, carrying out coaxial electrostatic spinning with a core layer spinning solution to obtain felt cloth, and dissolving the felt cloth in a core layer solvent or heating to remove a core layer material.

5. Use according to claim 4, wherein the biodegradable polymer is selected from one or more of polycaprolactone, polylactic acid-co-glycolic acid, L-polylactic acid, polyvinylpyrrolidone, poly (3-hydroxy-3-valerate), polyvinyl alcohol and chitosan.

6. Use according to claim 4, wherein the organic solvent is selected from one or more of chloroform, dichloromethane, hexafluoroisopropanol, trifluoroacetic acid, dimethylformamide, acetone and dimethyl sulfoxide.

7. The use according to claim 4, wherein the concentration of the skin layer dope is (6-20) wt%.

8. Use according to claim 4, wherein the core dope is one or more selected from water, liquid paraffin and mineral oil.

9. Use according to claim 4, characterized in that in said coaxial electrospinning, one or more of the following features are included:

the rotating speed of the roller is 500-5000 rpm;

the ejection speed of the skin layer spinning solution is 0.1-3.0 mL/h;

the ejection speed of the core layer spinning solution is 10-75% of that of the skin layer spinning solution;

the receiving distance is 3-30 cm;

applying a direct-current voltage of 10-50 KV;

the ambient temperature is 23-27 ℃;

the environmental humidity is 20-30%.

10. Use according to claim 4, wherein the core layer solvent is selected from one or more of cyclohexane, benzene, toluene, xylene and petroleum ether.

Technical Field

The invention relates to the field of non-woven fabrics, in particular to application of nano-fiber felt cloth in preparing bone defect repair products or bone formation promoting products.

Background

Bones are important components of the human body, have functions of protecting, providing exercise support, storing minerals and the like, and provide blood cells for the human circulatory system and the immune system. Bone defects caused by trauma, inflammation, tumor after operation and the like can cause partial form and function loss, and bring serious pain to patients. To date, clinical bone defects are in enormous cases and present an increasing trend year by year. The repair of bone defects is one of the problems to be solved clinically at present, especially in the field of repair of large-size, difficult-to-heal and pathological defects.

Bone grafting is the main method for clinically treating and repairing bone defects at present, and comprises autologous bone grafting and allogeneic bone grafting, but both have disadvantages. The autologous bone transplantation needs to open a second operation area, and has limited supply amount, thus easily causing complications such as chronic pain, infection and the like. The bone creeping replacement of the allogeneic bone transplantation is slow, collapse and fracture often occur at the late stage, and potential risks of disease transmission exist, so that the allograft bone transplantation is limited. The bone repair strategy taking exogenous electrical stimulation as a main body has important prospect in the aspect of clinical treatment and bone defect repair application. Compared with traditional chemical stimulation and drug therapy, the electrical stimulation method has the advantages of mild conditions, avoidance of chemical injury and drug side effects of organisms and the like. However, in the field of electrical stimulation regulation of bone tissue repair and regeneration in vivo, there are problems in that the limitation of an external energy supply system is broken, and the resistance to the impact of biomechanical energy is enhanced. Therefore, the development of a class of bone repair materials and devices capable of stimulating cell growth in situ and regulating osteogenic differentiation is one of the key paths for solving the bottleneck of clinical application.

Disclosure of Invention

In view of the above-mentioned drawbacks of the prior art, the present invention aims to provide a use of a nanofiber felt for preparing a bone defect repair product or an osteogenesis promoting product, for solving the technical problem of bone defect repair in the prior art.

To achieve the above objects and other related objects, the present invention includes the following technical solutions.

The invention provides application of nanofiber felt cloth in preparing bone defect repair products or osteogenesis promoting products.

According to the use, the nanofiber felt is a non-woven fabric composed of hollow nanofibers formed from a biodegradable polymer, and the nanofibers in the nanofiber felt have an orientation.

According to the application, the gram weight of the nanofiber felt is (1-15) g/m²。

According to the application, the nanofiber felt is prepared by adopting the following method: taking an organic solvent solution of a biodegradable polymer as a skin layer spinning solution, carrying out coaxial electrostatic spinning with a core layer spinning solution to obtain felt cloth, and dissolving the felt cloth in a core layer solvent or heating to remove a core layer material.

According to the above-mentioned use, the biodegradable polymer is selected from one or more of polycaprolactone (abbreviated as PCL), polylactic-co-glycolic acid (abbreviated as PLGA), levopolylactic acid (abbreviated as PLLA), polyvinylpyrrolidone (abbreviated as PVP), poly (3-hydroxy-3-valerate) (abbreviated as PHBV), polyvinyl alcohol (abbreviated as PVA) and chitosan. Preferably, the number average molecular weight of the biodegradable polymer is 20000-200000.

According to the above-mentioned use, the organic solvent is selected from one or more of chloroform, dichloromethane, hexafluoroisopropanol, trifluoroacetic acid, dimethylformamide (abbreviated as DMF), acetone and dimethylsulfoxide (abbreviated as DMSO).

According to the application, the concentration of the skin layer spinning solution is (6-20) wt%.

The use as described above, wherein the core layer spinning solution is one or more selected from the group consisting of water, liquid paraffin and mineral oil.

The coaxial electrostatic spinning technology in the application is as follows: the sheath spinning solution and the core layer spinning solution are respectively arranged in two different injectors, a spinning system consists of two coaxial capillaries with different inner diameters, the sheath spinning solution and the core layer spinning solution are converged under the action of a high-voltage electric field, and after the sheath spinning solution and the core layer spinning solution are stretched and received by a receiving device roller, a sheath-core structure fiber tow is formed and wound on the surface of the roller to form felt cloth, a core layer material of the felt cloth is dissolved and removed through a core layer solvent, a sheath layer material is left, and then the nano-fiber felt cloth formed by hollow fibers is obtained.

According to the application, in the coaxial electrostatic spinning, the rotating speed of a roller is 500-5000 rpm.

According to the application, in the coaxial electrostatic spinning, the ejection speed of the skin layer spinning solution is 0.1-3.0 mL/h.

According to the application, in the coaxial electrostatic spinning, the ejection speed of the core layer spinning solution is 10-75% of that of the skin layer spinning solution.

According to the application, in the coaxial electrostatic spinning, the receiving distance is 3-30 cm. The common receiving distance in this application refers to the distance between the outlet of the spinneret of the spinning system to the central axis of the drum of the receiving device.

According to the application, in the coaxial electrostatic spinning, a direct-current voltage of 10-50 KV is applied.

According to the application, in the coaxial electrostatic spinning, the environment temperature is 23-27 ℃.

According to the application, in the coaxial electrostatic spinning, the environment humidity is 20-30%.

According to the application, the solvent is mutually soluble with the core layer spinning solution and is not mutually soluble with the organic solvent in the skin layer spinning solution. Preferably, the core layer solvent is selected from one or more of cyclohexane, benzene, toluene and xylene.

The application can specifically be as follows: sterilizing the nanofiber felt cloth, cutting the nanofiber felt cloth into a size matched with the bone defect position, covering the surface of the bone defect position, keeping the orientation direction of the fibers basically consistent with the stretching direction of peripheral muscles, and sewing the nanofiber felt cloth at two ends of the muscles to ensure that the nanofibers can be stretched along the orientation direction when the muscles contract, thereby realizing the compression of the porous structure in the nanofibers. The sterilization treatment mode is as follows: soaking in 75% ethanol while irradiating with ultraviolet lamp. The treatment time is at least 2 h.

When the nanofiber felt is stretched by muscle orientation, the porous structure inside the nanofibers is compressed, the induction distance of the internal electret charges is shortened, the induced charges on the surfaces of the fibers are migrated, and therefore, a potential difference is generated between the upper surface and the lower surface of the nanofiber felt and a current is generated until new charge balance is achieved. When the muscle is recovered to a relaxed state, the compressed state of the porous structure in the nano fiber disappears, the internal electret charge induction distance is recovered, and the induced charges on the surface of the fiber generate reverse migration, so that reverse potential differences are generated on the upper surface and the lower surface of the nano fiber felt cloth, and reverse current is generated until new charge balance is achieved. The whole process is the process of collecting the mechanical energy generated by one muscle contraction and converting the mechanical energy into electric energy by the nano-fiber felt cloth.

The nano-fiber felt cloth collects the biomechanical energy of muscles and converts the biomechanical energy into electric energy, and is used for electrically stimulating cells at the bone defect part, particularly stem cells, to differentiate towards the osteogenic direction in situ, so as to realize the rapid and accurate repair of the bone defect.

The technical scheme of the invention has the beneficial effects that:

the technical scheme of the invention is that the nanofiber with an internal porous structure is constructed by a coaxial electrostatic spinning technology, and the electrical stimulation nanofiber felt cloth capable of promoting osteogenesis is obtained by orientation arrangement of the nanofiber. The nano-fiber felt cloth can effectively collect the biological mechanical energy and convert the biological mechanical energy into electric energy, is directly used for electrical stimulation of cells at a bone defect part, particularly stem cells, and realizes self-driven electrical stimulation osteogenesis of a host while an external energy supply system and a complex circuit are not needed.

Drawings

FIG. 1 is a micro-CT image of a blank group, a control group and an experimental group in example 1 of the present application

FIG. 2 is a bar graph showing data obtained after quantitative analysis of the micro-CT image of FIG. 1

Detailed Description

The following description of the embodiments of the present invention is provided for illustrative purposes, and other advantages and effects of the present invention will become apparent to those skilled in the art from the present disclosure.

Before the present embodiments are further described, it is to be understood that the scope of the invention is not limited to the particular embodiments described below; it is also to be understood that the terminology used in the examples is for the purpose of describing particular embodiments only, and is not intended to limit the scope of the present invention. Test methods in which specific conditions are not specified in the following examples are generally carried out under conventional conditions or under conditions recommended by the respective manufacturers.

When numerical ranges are given in the examples, it is understood that both endpoints of each of the numerical ranges and any value therebetween can be selected unless the invention otherwise indicated. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. In addition to the specific methods, devices, and materials used in the examples, any methods, devices, and materials similar or equivalent to those described in the examples may be used in the practice of the invention in addition to the specific methods, devices, and materials used in the examples, in keeping with the knowledge of one skilled in the art and with the description of the invention.