阅读说明：本技术 一种高油乳清粉的制备方法 (Preparation method of high-oil whey powder ) 是由 李红娟 赵树静 李丹 于景华 于洪梅 秦爱荣 李媛 于 2020-05-20 设计创作，主要内容包括：本发明提供了一种高油乳清粉的制备方法,所述高油乳清粉由乳清蛋白、调和油、脱盐乳清粉、和水组成,其制备方法通过对乳清蛋白进行热变性处理,将其作为乳化剂控制高油乳清粉的稳定特性,并在工艺过程中添加超声预乳化工段,最终制得产品稳定性、溶解性良好,包埋油滴效率及高油乳清粉复原乳稳定性提高。(The invention provides a preparation method of high-oil whey powder, which consists of whey protein, blend oil, desalted whey powder and water.)

1. A preparation method of high oil whey powder is characterized by comprising the following steps: the method comprises the following steps:

(1) heat denaturation of whey protein

Dissolving whey protein isolate in deionized water to obtain a whey protein solution with a protein concentration of 0.5-8% M/v, homogenizing, hydrating at 55-65 deg.C for 0.5-1h, maintaining at 80-90 deg.C for 10-30min, cooling to room temperature, and adjusting pH to 6.5-7.0 with 1M acid solution;

(2) preparation of high oil emulsion powder

Mixing the heat denaturation whey protein solution obtained in the step (1) with blend oil, dispersing and homogenizing at high speed, then carrying out ultrasonic emulsification treatment, adding desalted whey powder and water to prepare high oil emulsion powder liquid, and carrying out colloid mill, high-pressure homogenizer, sterilization and spray drying to obtain the finished product.

2. The method of claim 1, wherein the high oil whey powder is prepared by: the ultrasonic emulsification treatment conditions are set to be 800W at 300-.

3. The method of claim 1, wherein the high oil whey powder is prepared by: the high-speed dispersion homogenization is carried out for 5-10min under the conditions of 10,000 and 20,000 rpm.

4. A high oil whey powder prepared by the method of any one of claims 1 to 3, wherein: the components and weight percentage are as follows: whey protein isolate: 0.5-2%, blend oil: 8.75-13.75%, desalted whey powder D90: 10-17.5 percent, and the balance of water.

Technical Field

The invention belongs to the field of food, relates to high oil emulsion powder, and particularly relates to a preparation method of high oil emulsion powder.

Background

The high oil whey powder is an important raw material for producing infant formula milk powder by a dry method, and different vegetable oil sources provide fatty acid compositions which are closest to breast milk and most suitable for infants. Preparing emulsion according to the mixture ratio, and obtaining the high oil emulsion powder after shearing, emulsifying, spraying and drying. The shelf life of the dairy product can be improved by converting the dairy product into a powdery product through spray drying. The powder is easy to store, handle and transport.

In the production of high oil emulsion powder, the main problem is how to obtain stable oil-in-water emulsion, and only if oil-in-water emulsion with good stability is formed, the powdery high oil emulsion powder product with long-term stability can be prepared after a series of processes, and simultaneously, because the high oil emulsion powder contains a large amount of unsaturated fatty acid, the stable oil-in-water system can also effectively reduce the occurrence of fat oxidation problem. Therefore, it is very important to effectively embed the oil phase in the high oil whey powder. However, high oil whey powder is mainly used as a raw material for dry production of infant formula, and therefore, no stabilizer or emulsifier is added.

CN105230811B discloses a preparation method of high oil emulsion powder containing polyunsaturated fatty acid grease, which comprises the following steps:

preparation of mixed oil containing polyunsaturated fatty acid grease

Preparing 0.14kg of sunflower seed oil, 0.017kg of arachidonic acid oil (the content of arachidonic acid is 40%), 0.0094kg of docosahexaenoic acid oil (the content of docosahexaenoic acid is 35%), 0.012kg of ascorbyl palmitate, 0.036kg of vitamin E0.036kg and 0.036kg of phospholipid. Heating sunflower seed oil to 120 deg.C in round bottom flask, adding ascorbyl palmitate, vitamin E and phospholipid, and stirring for 6 min. Cooling the oil to 50 ℃, adding arachidonic acid oil and docosahexaenoic acid oil, maintaining the stirring speed of 30r/min, uniformly stirring to obtain an oil phase core material solution, and performing nitrogen charging protection in the whole process.

(2) Preparation of wall Material liquid

0.5kg of modified starch, 0.25kg of maltodextrin, 0.0005kg of ascorbic acid and 1kg of purified water are prepared. Heating pure water to 90 deg.C in a beaker, adding modified starch, maltodextrin and ascorbic acid, and shearing with a laboratory emulsifying machine at 8000r/min for 10min to obtain water phase wall material liquid.

(3) Preparation of emulsion rich in arachidonic acid and docosahexaenoic acid

Adding the oil phase core material solution into the water phase wall material solution for shearing, shearing at the speed of 8000r/min for 10min, and homogenizing under the pressure of 40MPa to obtain the nano-scale emulsion rich in arachidonic acid and docosahexaenoic acid.

(4) Preparation of high oil emulsion powder containing polyunsaturated fatty acid oil

And (3) pasteurizing the homogenized material liquid, and performing high-pressure spray drying to obtain the high-oil whey powder containing the polyunsaturated fatty acid grease.

The above patent prepares a blend oil formula rich in arachidonic acid and docosahexaenoic acid as an oil phase, and then embeds the oil phase with maltodextrin and modified starch as wall materials to prepare an emulsion. The main innovation point of the patent is that the whey protein is used as a wall material to be mixed with blending oil through treatment of the whey protein, and an ultrasonic process is added to prepare emulsion and high-oil emulsion powder products with good stability.

Disclosure of Invention

The invention aims to overcome the defects of the prior art and provides a preparation method of high-oil whey powder capable of improving stability.

The technical scheme adopted by the invention for solving the technical problem is as follows:

a preparation method of high oil whey powder comprises the following steps:

(1) heat denaturation of whey protein

Dissolving whey protein isolate in deionized water to obtain whey protein solution with protein concentration of 0.5-8% M/v, homogenizing, hydrating at 55-65 deg.C for 0.5-1h, maintaining at 80-90 deg.C for 10-30min, cooling to room temperature, and adjusting pH to 6.5-7.0 with 1M acid solution (citric acid, lactic acid, malic acid, hydrochloric acid, etc.);

(2) preparation of high oil emulsion powder

Mixing the heat denaturation whey protein solution obtained in the step (1) with blend oil, dispersing and homogenizing at high speed, then carrying out ultrasonic emulsification treatment, adding desalted whey powder and water to prepare high oil emulsion powder liquid, and carrying out colloid mill, high-pressure homogenizer, sterilization and spray drying to obtain the finished product.

Moreover, the ultrasonic emulsification treatment conditions are set to be 300-800W, 3s of ultrasonic treatment, 3s of gap treatment and 10-30min of total time.

Furthermore, the high-speed dispersion homogenization is carried out for 5-10min under the conditions of 10,000-20,000 rpm.

The invention also protects the high-oil whey powder prepared by the method, which comprises the following components in percentage by weight: 0.5-2%, blend oil: 8.75-13.75%, desalted whey powder D90: 10-17.5 percent, and the balance of water.

The invention has the advantages and beneficial effects that:

the invention carries out thermal denaturation treatment on the lactalbumin, uses the lactalbumin as a system emulsifier, embeds the oil phase to generate an oil-in-water emulsion, forms a stable emulsification system, can reduce the problems of fat oxidation, particle damage and the like, and has good application prospect.

Drawings

FIG. 1 shows the measurement results of different particle sizes of high oil whey powder;

FIG. 2 shows the results of the determination of the solubility of different high oil whey;

FIG. 3 shows the measurement results of the oil content of the surface of different high oil whey powders;

FIG. 4(a) is a 500-fold electron micrograph of a control sample;

FIG. 4(b) is a 3000 XSEM photograph of a control sample;

FIG. 4(c) is a 500-fold SEM of sample No. 1;

FIG. 4(d) is a 3000 XSEM of sample No. 1;

FIG. 4(e) is a 500-fold SEM of sample No. 2;

FIG. 4(f) is a 3000 XSEM image of sample No. 2;

FIG. 4(g) is a 500-fold electron micrograph of sample No. 3;

FIG. 4(h) is a 3000 times electron micrograph of sample No. 3;

FIG. 4(i) is a 500-fold electron micrograph of sample No. 4;

FIG. 4(j) is a 3000 XSEM of sample No. 4;

FIG. 5 shows the determination results of TSI (dynamic stability index) of different high oil whey powders.

Detailed Description

The present invention is further illustrated by the following specific examples, which are intended to be illustrative, not limiting and are not intended to limit the scope of the invention.

TABLE 1 high oil whey powder liquid formulation

Comparative example 1

Raw materials: 8.75 percent of blend oil, 8.75 percent of desalted whey powder D9016.25 percent and 75 percent of water

The method comprises the following steps: mixing the above materials, and grinding with colloid mill, high pressure homogenizer, spray drying, etc. to obtain the final product.