阅读说明：本技术 一种用于抗炎镇痛的药物组合物及其制备方法 (Pharmaceutical composition for resisting inflammation and easing pain and preparation method thereof ) 是由 伏莹 韩晓霞 范明月 于 2020-06-04 设计创作，主要内容包括：本发明提供一种用于抗炎镇痛的药物组合物及其制备方法。具体提供一种优化的那如-3药材的提取工艺,并对不同提取方法得到的膏状物的抗炎镇痛药理活性进行了评价,最终筛选出三种最优的提取工艺,其制备获得的药物组合物能够在抗炎镇痛方面具有更佳的疗效,同时其提取工艺简便易行,更适合于工业化生产。(The invention provides a pharmaceutical composition for resisting inflammation and easing pain and a preparation method thereof. The method specifically provides an optimized extraction process of the Nae-3 medicinal material, the anti-inflammatory analgesic pharmacological activity of the paste obtained by different extraction methods is evaluated, three optimal extraction processes are finally screened, the pharmaceutical composition prepared by the method has better curative effect on anti-inflammatory analgesic, and meanwhile, the extraction process is simple, convenient and feasible and is more suitable for industrial production.)

1. A preparation method of a pharmaceutical composition for anti-inflammatory and analgesic purposes is characterized by comprising the following steps:

(1) weighing myrobalan, long pepper and prepared kusnezoff monkshood root respectively, crushing into coarse powder, putting the coarse powder into a decoction container, adding water with 6 times of the total amount of medicinal materials, heating to boil for 1.5 hours, and pouring decoction for later use;

(2) and (3) decocting twice by the same method, removing decoction dregs, mixing decoction, concentrating decoction until the relative density is 1.16-1.21 (measured at 55-60 ℃) to obtain an extract, and weighing to obtain the traditional Chinese medicine.

2. A preparation method of a pharmaceutical composition for anti-inflammatory and analgesic purposes is characterized by comprising the following steps:

(1) weighing myrobalan, long pepper and prepared kusnezoff monkshood root respectively, crushing into coarse powder, putting the coarse powder into a decoction container, adding 3% acetic acid solution with 6 times of the total amount of medicinal materials, heating to boil for 3 hours, and pouring decoction for later use;

(2) decocting twice by the same method, removing residues, mixing decoctions, concentrating the decoction to obtain an extract with a relative density of 1.16-1.21 (measured at 55-60 ℃), and weighing;

(3) alcohol precipitation operation: and (3) preparing the extract decocted and concentrated in the step (2) into an alcohol solution containing 80% ethanol by using 95% ethanol, heating, concentrating under reduced pressure to obtain an extract with the relative density of 1.16-1.21 (measured at 55-60 ℃), and weighing to obtain the traditional Chinese medicine composition.

3. A preparation method of a pharmaceutical composition for anti-inflammatory and analgesic purposes is characterized by comprising the following steps:

weighing myrobalan, long pepper and prepared kusnezoff monkshood root respectively, crushing into coarse powder, placing into a reflux extractor, adding 75% ethanol with the amount of 6 times of the total amount of medicinal materials, refluxing for three times, 1.5 hours each time, combining extracting solutions, recovering ethanol, concentrating to obtain an extract with the relative density of 1.16-1.21 (measured at 55-60 ℃), and weighing to obtain the traditional Chinese medicine composition.

4. A pharmaceutical composition for anti-inflammatory and analgesic use, which is prepared by the method according to any one of claims 1 to 3.

5. The pharmaceutical composition of claim 4, wherein: the pharmaceutical composition can be added with conventional auxiliary materials and prepared into an external preparation according to a conventional process.

6. A preparation method of a plaster for resisting inflammation and easing pain is characterized by comprising the following steps:

(1) weighing myrobalan, long pepper and prepared kusnezoff monkshood root respectively, crushing into coarse powder, putting the coarse powder into a decoction container, adding water with 6 times of the total amount of medicinal materials, heating to boil for 1.5 hours, and pouring decoction for later use;

(2) decocting twice by the same method, removing residues, mixing decoctions, concentrating the decoction until the relative density is 1.16-1.21 (measured at 55-60 ℃), weighing;

(3) adding the extract prepared in the step (2) into conventional plaster auxiliary materials, and preparing into plaster according to the preparation process of the conventional plaster preparation.

7. A preparation method of a plaster for resisting inflammation and easing pain is characterized by comprising the following steps:

(1) weighing myrobalan, long pepper and prepared kusnezoff monkshood root respectively, crushing into coarse powder, putting the coarse powder into a decoction container, adding 3% acetic acid solution with 6 times of the total amount of medicinal materials, heating to boil for 3 hours, and pouring decoction for later use;

(2) decocting twice by the same method, removing residues, mixing decoctions, concentrating the decoction to obtain an extract with a relative density of 1.16-1.21 (measured at 55-60 ℃), and weighing;

(3) alcohol precipitation operation: preparing the extract decocted and concentrated in the step (2) into an alcohol solution containing 80% ethanol by using 95% ethanol, heating, concentrating under reduced pressure to obtain an extract with the relative density of 1.16-1.21 (measured at 55-60 ℃), and weighing;

(4) adding the extract prepared in the step (3) into conventional plaster auxiliary materials, and preparing into plaster according to the preparation process of the conventional plaster preparation.

8. A preparation method of a plaster for resisting inflammation and easing pain is characterized by comprising the following steps:

(1) weighing myrobalan, long pepper and prepared kusnezoff monkshood root respectively, crushing into coarse powder, placing into a reflux extractor, adding 75% ethanol with the amount 6 times of the total amount of medicinal materials, refluxing for three times, 1.5 hours each time, combining extracting solutions, recovering ethanol, concentrating to obtain an extract with the relative density of 1.16-1.21 (measured at 55-60 ℃), and weighing;

(2) adding the extract prepared in the step (1) into conventional plaster auxiliary materials, and preparing into plaster according to the preparation process of the conventional plaster preparation.

9. A patch for anti-inflammatory and analgesic use, which is prepared by the method of any one of claims 6 to 8.

Technical Field

The invention relates to the technical field of medicines, in particular to a pharmaceutical composition for resisting inflammation and easing pain and a preparation method thereof.

Background

The Mongolian medicine, such as-3, originally is a Mongolian medicine oral preparation, has obvious curative effects on eliminating 'viscidity', removing 'synergistic sunus essence', dispelling wind, relieving pain and dispelling cold. However, the oral medicine is inconvenient to use and can not directly reach the affected part, and the treatment effect is slow. Years of research of external plaster experts show that the effect of the medicine prepared into the external plaster preparation is obvious. Therefore, the traditional medicine taking mode of the nation is combined with the modern advanced technology, and the Mongolian medicine product of external preparation type which has simple operation, convenient use, high bioavailability, no absorption by liver and kidney, small toxic and side effect and no damage to liver and kidney and is accepted by patients is researched, developed and produced, and is the research and development power of the new medicine.

However, in the development process of Mongolian medicine, such as-3 plaster, the extraction of medicinal materials is the key of the preparation process, the clinical curative effect of the medicine is influenced, and the best curative effect of the medicine can be achieved only by selecting a good extraction process. In patent application CN 201910219007.5 previously filed by the applicant, an anti-inflammatory analgesic plaster of naf-3 is disclosed, which is prepared by using a method of extracting piper longum with alcohol and extracting myrobalan and radix aconiti kusnezoffii with water at the same time, however, the extraction process needs to group the raw materials in the production process, which increases the production cost. Therefore, the optimization research on the extraction process of the medicinal materials of the plaster-3 is needed to obtain a medicinal composition with better anti-inflammatory and analgesic effects and simpler production process.

Disclosure of Invention

The invention aims to solve the technical problem of optimizing the extraction process of the medicinal material, namely-3, so as to provide a medicinal composition with better anti-inflammatory and analgesic effects and a preparation method thereof.

Therefore, according to the physicochemical properties and pharmacological effects of the main chemical components of the three medicinal materials in the prescription as-3, six extraction processes are designed, the anti-inflammatory analgesic pharmacological activities of the pastes obtained by different extraction methods are evaluated, the influence of the pastes obtained by different extraction processes on the analgesic effect of a mouse and the anti-inflammatory effect of a rat is considered, and three optimal extraction processes are finally screened.

Specifically, the invention provides a preparation method of a pharmaceutical composition for anti-inflammatory and analgesic, which comprises the following steps:

(1) weighing myrobalan, long pepper and prepared kusnezoff monkshood root respectively, crushing into coarse powder, putting the coarse powder into a decoction container, adding water with 6 times of the total amount of medicinal materials, heating to boil for 1.5 hours, and pouring decoction for later use;

(2) and (3) decocting twice by the same method, removing decoction dregs, mixing decoction, concentrating decoction until the relative density is 1.16-1.21 (measured at 55-60 ℃) to obtain an extract, and weighing to obtain the traditional Chinese medicine.

The invention also provides a preparation method of the pharmaceutical composition for resisting inflammation and easing pain, which comprises the following steps:

(1) weighing myrobalan, long pepper and prepared kusnezoff monkshood root respectively, crushing into coarse powder, putting the coarse powder into a decoction container, adding 3% acetic acid solution with 6 times of the total amount of medicinal materials, heating to boil for 3 hours, and pouring decoction for later use;

(2) decocting twice by the same method, removing residues, mixing decoctions, concentrating the decoction to obtain an extract with a relative density of 1.16-1.21 (measured at 55-60 ℃), and weighing;

(3) alcohol precipitation operation: and (3) preparing the extract decocted and concentrated in the step (2) into an alcohol solution containing 80% ethanol by using 95% ethanol, heating, concentrating under reduced pressure to obtain an extract with the relative density of 1.16-1.21 (measured at 55-60 ℃), and weighing to obtain the traditional Chinese medicine composition.

The invention also provides a preparation method of the pharmaceutical composition for resisting inflammation and easing pain, which comprises the following steps:

weighing myrobalan, long pepper and prepared kusnezoff monkshood root respectively, crushing into coarse powder, placing into a reflux extractor, adding 75% ethanol with the amount of 6 times of the total amount of medicinal materials, refluxing for three times, 1.5 hours each time, combining extracting solutions, recovering ethanol, concentrating to obtain an extract with the relative density of 1.16-1.21 (measured at 55-60 ℃), and weighing to obtain the traditional Chinese medicine composition.

In another aspect, the invention provides a pharmaceutical composition for anti-inflammatory and analgesic purposes, which is prepared by the method.

Preferably, the pharmaceutical composition can be added with conventional auxiliary materials and prepared into an external preparation according to a conventional process.

The invention further provides a preparation method of the plaster for resisting inflammation and easing pain, which comprises the following steps:

(1) weighing myrobalan, long pepper and prepared kusnezoff monkshood root respectively, crushing into coarse powder, putting the coarse powder into a decoction container, adding water with 6 times of the total amount of medicinal materials, heating to boil for 1.5 hours, and pouring decoction for later use;

(2) decocting twice by the same method, removing residues, mixing decoctions, concentrating the decoction until the relative density is 1.16-1.21 (measured at 55-60 ℃), weighing;

(3) adding the extract prepared in the step (2) into conventional plaster auxiliary materials, and preparing into plaster according to the preparation process of the conventional plaster preparation.

The invention also provides a preparation method of the plaster for resisting inflammation and easing pain, which comprises the following steps:

(1) weighing myrobalan, long pepper and prepared kusnezoff monkshood root respectively, crushing into coarse powder, putting the coarse powder into a decoction container, adding 3% acetic acid solution with 6 times of the total amount of medicinal materials, heating to boil for 3 hours, and pouring decoction for later use;

(2) decocting twice by the same method, removing residues, mixing decoctions, concentrating the decoction to obtain an extract with a relative density of 1.16-1.21 (measured at 55-60 ℃), and weighing;

(3) alcohol precipitation operation: preparing the extract decocted and concentrated in the step (2) into an alcohol solution containing 80% ethanol by using 95% ethanol, heating, concentrating under reduced pressure to obtain an extract with the relative density of 1.16-1.21 (measured at 55-60 ℃), and weighing;

(4) adding the extract prepared in the step (3) into conventional plaster auxiliary materials, and preparing into plaster according to the preparation process of the conventional plaster preparation.

The invention also provides a preparation method of the plaster for resisting inflammation and easing pain, which comprises the following steps:

(1) weighing myrobalan, long pepper and prepared kusnezoff monkshood root respectively, crushing into coarse powder, placing into a reflux extractor, adding 75% ethanol with the amount 6 times of the total amount of medicinal materials, refluxing for three times, 1.5 hours each time, combining extracting solutions, recovering ethanol, concentrating to obtain an extract with the relative density of 1.16-1.21 (measured at 55-60 ℃), and weighing;

(2) adding the extract prepared in the step (1) into conventional plaster auxiliary materials, and preparing into plaster according to the preparation process of the conventional plaster preparation.

Furthermore, the invention also provides a plaster for resisting inflammation and easing pain, which is prepared by the method.

The following will describe the embodiment of the extraction process screening and the effect thereof according to the present invention with reference to specific experimental examples and examples. It should be understood, however, that the description is for illustrative purposes only and is not intended to limit the claims of the present invention in any way.

Detailed Description

Experimental example 1: different extraction process designs of the pharmaceutical composition of the invention

The test adopts three solvent extraction technologies of water, acid water and ethanol, and adopts 6 technologies of water decoction, acid water decoction and alcohol precipitation, 90% ethanol reflux, 75% ethanol reflux and 30% ethanol reflux.

1. The first process comprises the following steps: water decoction extraction process

1.1 test prescription

600g myrobalan, 180g long pepper, 300g prepared kusnezoff monkshood root

Total amount total: 1080g

1.2, the preparation method comprises the following steps: weighing the myrobalan, the long pepper and the prepared kusnezoff monkshood root according to the prescription amount respectively, crushing into coarse powder, putting into a decoction container, adding 6480ml (6 times of the total amount of the medicinal materials) of water, heating to boil for 1.5 hours, and pouring out the decoction for later use. And (3) decocting twice by the same method, removing dregs, mixing decoctions, concentrating the decoction until the relative density is 1.16-1.21 (measured at 55-60 ℃) to obtain an extract, and weighing.

2. And a second process: acid water decoction extraction process

2.1 test prescription:

600g myrobalan, 180g long pepper, 300g prepared kusnezoff monkshood root

Total amount total: 1080g

2.2, a preparation method: weighing fructus Chebulae, fructus Piperis Longi, and radix Aconiti Kusnezoffii Preparata respectively, pulverizing into coarse powder, placing in a decocting container, adding 3% acetic acid solution 6480ml, heating to boil for 1.5 hr, and collecting the decoction. And (3) decocting twice by the same method, removing dregs, mixing decoctions, and concentrating the decoction until the relative density is 1.16-1.21 (measured at 55-60 ℃) to obtain an extract.

3. And a third process: process for extracting by decocting in acid water and depositing in alcohol

3.1 test prescription:

600g myrobalan, 180g long pepper, 300g prepared kusnezoff monkshood root

Total amount total: 1080g

3.2, a preparation method: weighing fructus Chebulae, fructus Piperis Longi, and radix Aconiti Kusnezoffii Preparata respectively, pulverizing into coarse powder, placing in a decocting container, adding 3% acetic acid solution 6480ml (6 times of the total amount of the medicinal materials), heating to boil for 3 hr, and collecting the decoction. Decocting for 2 times by the same method, finally removing decoction dregs, combining decoction, concentrating decoction until the relative density is 1.16-1.21 (measured at 55-60 ℃) to obtain extract, and weighing. Then carrying out alcohol precipitation operation: and (3) preparing the decocted and concentrated extract into an alcohol solution containing 80% ethanol by using 95% ethanol, heating, concentrating under reduced pressure to obtain an extract with the relative density of 1.16-1.21 (measured at 55-60 ℃), and weighing.

4. And a fourth process: 90% ethanol reflux extraction process

4.1 test prescription:

600g myrobalan, 180g long pepper, 300g prepared kusnezoff monkshood root

Total amount total: 1080g

4.2, preparation method: weighing myrobalan, long pepper and prepared kusnezoff monkshood root respectively, crushing into coarse powder, putting into a reflux extractor, adding 90% ethanol 6480ml (6 times of the total amount of the medicinal materials), refluxing for three times, refluxing for 1.5 hours each time, combining extracting solutions, recovering ethanol, and concentrating to obtain an extract with the relative density of 1.16-1.21 (measured at 55-60 ℃ by heat).

5. And a fifth process: reflux extraction process of 75% ethanol

5.1 test prescription:

600g myrobalan, 180g long pepper, 300g prepared kusnezoff monkshood root

Total amount total: 1080g

5.2, a preparation method: weighing myrobalan, long pepper and prepared kusnezoff monkshood root respectively, crushing into coarse powder, placing into a reflux extractor, adding 6480ml of 75% ethanol (6 times of the total amount of the medicinal materials), refluxing for three times, each time for 1.5 hours, combining extracting solutions, recovering ethanol, concentrating to obtain an extract with the relative density of 1.16-1.21 (measured at 55-60 ℃), and weighing.

6. And a sixth process: 30% ethanol reflux extraction process

6.1 test prescription:

600g myrobalan, 180g long pepper, 300g prepared kusnezoff monkshood root

Total amount total: 1080g

6.2, a preparation method comprises the following steps: weighing myrobalan, long pepper and prepared kusnezoff monkshood root respectively, crushing into coarse powder, placing into a reflux extractor, adding 30% ethanol 6480ml (6 times of the total amount of the medicinal materials), refluxing for three times, each time for 1.5 hours, combining extracting solutions, recovering ethanol, concentrating to obtain an extract with the relative density of 1.16-1.21 (measured at 55-60 ℃), and weighing.

Experimental example 2: pharmacodynamic research of different extraction processes of the pharmaceutical composition of the invention

1. The purpose of the test is as follows: the influence of the six extraction processes in the experimental example 1 on the analgesic effect of the mouse and the anti-inflammatory effect of the rat is discussed, and reference is further provided for the process synthetic route.

2. Test and reference substances

2.1 test article

Name: naru-3 plaster

Batch number: 18001. 18002, 18003, 18004, 18005, 18006

Specification: 300g

Preparation and physical state: the brown paste prepared by the six processes in the experimental example 1 is adopted

Providing a unit: inmongol Colqin pharmaceutical Co Ltd

Storage conditions are as follows: storing at 2-4 deg.C

2.2 control

The name is 0.9 percent sodium chloride injection

The ingredients are 1, active ingredients: sodium chloride

2. Auxiliary materials: hydrochloric acid, water for injection

Physical properties: colorless clear liquid

Specification: 250mL of: 2.25g

Storage conditions are as follows: sealed preservation

Batch number: s17113505-2

The manufacturer: jilin kang Nael limited public of pharmaceutical industry

2.3 Positive control 1

Name: diclofenac diethylamine emulsoid (trade name: hibilin)

Specification: 1% (50 g: 0.5 g, calculated as diclofenac sodium)

Batch number: VS4H

The manufacturer: GSK Consumer Healthcare Schweiz AG

2.4 Positive control 2

Name: compound dexamethasone acetate cream (trade name: pentacyclic brand)

Specification: 20g, 15 mg/piece

Batch number: 180406

The manufacturer: guangdong Tai En kang pharmaceutical Co., Ltd

3. Materials and methods

3.1 Main instrumentation

Name: electronic balance

The model is as follows: QUINTIX224-1CN

The manufacturer: sidolis Corp

3.2 test System

3.2.1 test System 1

Name of mouse

Strain Kunming

Class SPF class

Providing a unit: liaoning Biotechnology Ltd

Animal license number: SCXK (Liao) 2015-0001-

Animal certification number: no.211002300040245

Line selection reasons are as follows: kunming mice are the rodents which are commonly used for the tests, have stable genetic characters and clear background data, and are sensitive to the test products.

Number and sex of animals: 90 males were introduced, 80 were used, and the remaining 10 were used for pre-testing.

Age: 7-8 weeks old when introduced

Health examination and adaptation: the health status of the animals introduced was checked by the veterinarian on the day of introduction.

3.2.2 test system 2:

name: rat

Strain: SD

Grade: SPF stage

Providing a unit: liaoning Biotechnology Ltd

Animal license number: SCXK (Liao) 2015-0001-

Animal certification number: no.211002300041011

Line selection reasons are as follows: SD rats are rodents which are commonly used for carrying out the tests, have stable genetic characters and clear background data, and are sensitive to the test samples.

Number and sex of animals: 85 males were introduced, 80 were used, and the remaining 5 were used for pre-testing.

Age: 7-8 weeks old when introduced

Health examination and adaptation: on the day of animal introduction, health conditions of the animals introduced were examined by veterinarians.

3.3 test conditions

The test site comprises: this center is a short term toxicological animal house.

Feeding conditions are as follows: in a laboratory of a building A of the mechanism of a test site, big and small mice are all raised in a specific raising cage, 5 mice are raised in each box, and a single rat cage is raised. The animal cages, the stainless steel hampers and the water bottles are disinfected regularly.

Environmental conditions: the temperature and humidity of the animal room are automatically controlled, the temperature is recorded every day, the temperature is controlled to be 16-26 ℃, the humidity is controlled to be 40% -70% of artificial illumination, the light period is automatically controlled, the light is bright for 12 hours, the light is dark for 12 hours, and the noise is below 60 dB.

Ingestion and drinking: the animal feed is special feed for rats and mice. The nutrient content and the pollutant content of the feed meet the national standard. The drinking water is purified water prepared by the HT-R01000 type water purification system in the center, the water quality is detected once a year, and the detection indexes meet the national standard. The animals were allowed free access to drinking water during the acclimation period and the test period. The feed and water did not contain any contaminants that affected the purpose, integrity and results of the test.

Animal welfare: animal use followed the national animal welfare regulations guiding comments on good-care animals (2006, department of science). Animal use protocols were approved by the institutional animal care committee (IACUC) and the experimental procedures were under their supervision. At the end of the test, the surviving animals were euthanized with carbon dioxide and the carcasses were processed by the department of laboratory technology, entrusted to professional companies.

3.4 test methods

3.4.1 mouse Acetowrithing method for screening analgesic effect

80 Kunming white mice are adopted and divided into 8 groups of a control group, a positive medicine group, a process. Each group contained 10 animals. The remaining mice outside the model group were shaved off abdominal hair the day before the official trial. All administration groups were administered once on the day of the official test, and the application range was an approximately circular area having a diameter of about 1.5cm, and the administration volumes were all 0.2mL, and were applied to the same position of the abdomen. Dipping a little warm water by a cotton ball about half an hour after administration to wipe off the medicine, injecting 0.2mL of 0.6% glacial acetic acid solution into the abdominal cavity of each animal, continuously observing for 10 minutes, and recording the times of writhing of the mouse within 10 minutes; carrying out writhing observation on the control group of mice after directly injecting 0.6% glacial acetic acid; the positive control drug was applied to the abdomen of mice with commercially available diclofenac diethylamine emulsion (trade name: nataline), and the rest was performed as above.

3.4.2 screening anti-inflammatory effect of rat cotton ball granuloma method

80 SD rats are adopted and divided into 8 groups including a control group, a positive medicine group, a process group, a. Each group contained 10 animals. Before the start of the experiment, a number of cotton balls with a weight of about 20mg and a number of surgical instruments were prepared and autoclaved for future use. After anesthetizing, rats were shaved on the shoulder and neck, sterilized with iodophor, a small subcutaneous incision was cut, a sterilized cotton ball was placed under the shoulder and neck skin (the weight of the cotton ball placed before placement was weighed and recorded), and the incision was closed. The drug administration is started on the molding day, once a day and continuously carried out for 10 days, the positive drug is coated on the skin of the cotton ball placing part with a commercially available compound dexamethasone acetate ointment external preparation, each process group is coated with corresponding cream (0.35 g of extract in terms of dry matter) on the skin of the cotton ball placing part, and the control group is coated with physiological water. After the end of the administration, the cotton ball was euthanized, carefully peeled off and removed from the implant, and dried in an oven at about 60 ℃.

After the cotton balls are dried, the weight of each cotton ball is weighed, and the weight of each cotton ball before the cotton balls are implanted is subtracted to obtain the net weight of granuloma.

3.5 data processing and results assessment

The data are summarized in a table form, and the times of mouse writhing and the weight of cotton ball granuloma between different groups are counted by adopting a chi-square test and a T test respectively in a mouse acetic acid writhing test and a rat granuloma test.

4. Results

4.1 mouse acetic acid writhing test results

The number of writhing times of each group and the control group is compared, and the analgesic effect of the test object is expressed by the analgesic inhibition rate. The analgesic inhibition rate is (number of writhing in control group-number of writhing in administration group)/number of writhing in control group. Wherein, the positive drugs, the first process, the second process, the third process, the fourth process and the fifth process have significant differences in the times of writhing compared with the control group, and the results of each group are shown in the table 1 (the experimental research number is PE19003 RI).

TABLE 1 mouse acetic acid writhing test the number of writhing for each group (mean soil standard deviation)

Group of	Number of times of body twisting	Analgesic inhibitory Rate (%)
			Control group	31.33±7.75	--
Positive drug group	12.89±3.30*	58.87
			Process group	22.78±6.20*	27.30
Two groups of processes	20.17±8.42*	35.64
			Art three groups	19.75±8.92*	36.97
Art four groups	18.78±3.96*	40.07
			Art five groups	20.50±6.00*	34.57
Six groups of craft	23.86±6.89	23.86

Note: represents significant difference p <0.05 from control group

4.2 rat Cotton ball granuloma test results

The anti-inflammatory effect of the test subjects was expressed as the weight of cotton bud granuloma when each group was compared with the control group. Weight of granuloma of cotton ball (mg) after removal-weight of cotton ball before implantation. Compared with the control group, the positive medicine, the first process, the third process and the fifth process have significant difference in weight of cotton ball granuloma, and the results of each group are shown in table 2.

TABLE 2 weight of cotton ball granuloma (mg) for each group

Note: represents significant difference p <0.05 from control group

As can be seen from the experimental results in tables 1 and 2, the anti-inflammatory analgesic effect of the pharmaceutical composition of the present invention was observed through the study of different extraction processes, and compared with the control group, the analgesic effect of the pharmaceutical composition of the present invention is better in the first to fifth processes; in the aspect of anti-inflammatory effect, the pharmaceutical composition prepared by the first, third and fifth processes has better effect. The results are combined to obtain that the pharmaceutical composition prepared by the first, third and fifth processes has better curative effect on anti-inflammatory and analgesic effects, and the extraction process is simple, convenient and feasible and is more suitable for industrial production.