阅读说明：本技术 一种棉铃虫核型多角体病毒悬浮剂 (Heliothis armigera nuclear polyhedrosis virus suspending agent ) 是由 李广春 李雪松 程鹏 于 2019-01-25 设计创作，主要内容包括：本发明公开了一种棉铃虫核型多角体病毒悬浮剂及其制备方法,其组成原料及重量百分数如下：5000亿PIB/克棉铃虫核型多角体病毒母药0.42％、油酸甲酯10％、苯乙基苯酚聚氧乙烯醚6％、抗氧剂BHT1.5％、防腐剂苯甲酸钠0.5％,12-烷基硫酸钠3％、木质素磺酸钠2％、白炭黑1.5％、硅酸镁铝2％、黄原胶0.25％、有机硅消泡剂0.2％,余量为去离子水。本发明提供的棉铃虫核型多角体病毒悬浮剂所含5000亿棉铃虫核型多角体病毒0.42％,病毒粒子含量高,用量少,对防治棉花棉铃虫有较好的效果,其制备工艺简单,成本低,便于推广应用。(The invention discloses a cotton bollworm nuclear polyhedrosis virus suspending agent and a preparation method thereof, and the suspending agent comprises the following raw materials in percentage by weight: 0.42 percent of 5000 hundred million PIB/gram of cotton bollworm nuclear polyhedrosis virus mother drug, 10 percent of methyl oleate, 6 percent of phenethylphenol polyoxyethylene ether, 1.5 percent of antioxidant BHT, 0.5 percent of preservative sodium benzoate, 3 percent of 12-alkyl sodium sulfate, 2 percent of sodium lignin sulfonate, 1.5 percent of white carbon black, 2 percent of magnesium aluminum silicate, 0.25 percent of xanthan gum, 0.2 percent of organic silicon defoamer and the balance of deionized water. The cotton bollworm nuclear polyhedrosis virus suspending agent provided by the invention contains 0.42% of 5000 hundred million cotton bollworm nuclear polyhedrosis viruses, has high virus particle content and low dosage, has a good effect of preventing and treating cotton bollworms, and is simple in preparation process, low in cost and convenient to popularize and apply.)

1. The cotton bollworm nuclear polyhedrosis virus suspending agent is characterized by comprising the following raw materials in percentage by weight: 0.42 percent of 5000 hundred million PIB/gram of cotton bollworm nuclear polyhedrosis virus mother drug, 10 percent of methyl oleate, 6 percent of phenethylphenol polyoxyethylene ether, 1.5 percent of antioxidant BHT, 0.5 percent of preservative sodium benzoate, 3 percent of 12-alkyl sodium sulfate, 2 percent of sodium lignosulfonate, 1.5 percent of white carbon black, 2 percent of magnesium aluminum silicate, 0.25 percent of xanthan gum, 0.2 percent of organic silicon defoamer and the balance of deionized water.

2. A method for preparing a Heliothis armigera nuclear polyhedrosis virus suspension according to claim 1, comprising the steps of:

the method comprises the following steps: weighing the required auxiliary agents according to the weight percentage, putting the auxiliary agents into a preparation kettle, and premixing.

Step two: the raw medicine, the auxiliary agent and the filler are sequentially put into a preparation kettle to be stirred and mixed.

Step three: and starting a shearing machine to shear at a high speed, timing, and requiring that the mixing and shearing time is more than or equal to 40 minutes to fully and uniformly mix the materials.

Step four: the material is sent into a sand mill, and the sand mill and a water cooler are started (the sand milling temperature is less than or equal to 38 ℃). And (3) controlling the flow (the sanding time of each ton of the material is not less than 2.5 hours), observing the viscosity, the color and the dispersity of the sanded material, and controlling the fineness of the material (if the fineness does not reach the standard, the flow of the sanding machine can be readjusted, and the sanding time is increased until the fineness is qualified).

Step five: pumping the materials into a mixing kettle for remixing for more than or equal to 30 minutes.

Step six: sampling and testing. The content of the Heliothis armigera nuclear polyhedrosis virus is more than or equal to 20 hundred million PIB/miL; the unit of biological activity is more than or equal to 5000Ha U/microliter; the light stability of NPV is more than or equal to 30.0 percent; the suspension rate is more than or equal to 85 percent; pourability: qualified; the pH value is 6.0-7.0; the screen analysis (passing through a standard screen with the aperture of 45 microns) is more than or equal to 95 percent; thermal storage stability: and (4) passing. All technical indexes meet the requirements and are qualified products.

Step seven: and packaging, and warehousing the packaged product after the packaged product is qualified through sampling inspection.

3. The Heliothis armigera nuclear polyhedrosis virus suspending agent according to claims 1-2, wherein the adjuvant comprises methyl oleate, phenethylphenol polyoxyethylene ether, antioxidant BHT, preservative sodium benzoate, surfactant sodium dodecyl benzene sulfonate, sodium lignosulfonate, filler white carbon black, thickener xanthan gum, magnesium aluminum silicate, defoamer silicone, and the diluent is deionized water.

Technical Field

The invention relates to the field of novel biopesticides, and in particular relates to a cotton bollworm nuclear polyhedrosis virus suspending agent.

Background

The Heliothis armigera nuclear polyhedrosis virus belongs to baculovirus family, and consists of double-stranded DNA, protein and trace elements, and virus particles are single-particle embedded. The bollworm is a main pest which is harmful to the growth of cotton, and is mainly controlled by chemical insecticides at present. The chemical pesticide is toxic to human and livestock, damages natural enemies, pollutes the environment and can generate drug resistance after long-term use of the cotton bollworm. The prevention and treatment effect is reduced.

The bollworm nuclear polyhedrosis virus suspending agent is a novel biological pesticide and is prepared from nuclear polyhedrosis virus (NVP), a protective agent and other auxiliary materials. Pests infect NVP by feeding, under the action of intestinal juice of the pests, the inclusion body (PIB) protein is disintegrated, virus particles with infection capacity are released, the virus particles invade midgut epithelial cells to enter hemolymph, and are propagated in tissues such as trachea basement membrane, fat body and the like to gradually infect cells of the whole body of the pests, and the tissues of the whole body of the pests are pustulated to cause death. The product is nontoxic to human and livestock, does not harm natural enemies, does not pollute the environment, and does not generate resistance to cotton bollworms after long-term use. Therefore, the development of the cotton bollworm nuclear polyhedrosis virus suspending agent which has high content, high activity, good stability and good prevention and treatment effect is necessary.

Disclosure of Invention

In view of the defects of the existing cotton bollworm nuclear polyhedrosis virus suspending agent, the invention provides a cotton bollworm nuclear polyhedrosis virus suspending agent with less medicament dosage and good insecticidal effect, and the specific technical scheme is as follows:

the cotton bollworm nuclear polyhedrosis virus suspending agent comprises the following raw materials in percentage by weight: 0.42 percent of 5000 hundred million PIB/gram of cotton bollworm nuclear polyhedrosis virus mother drug, 10 percent of auxiliary agent methyl oleate, 6 percent of phenethyl phenol polyoxyethylene ether, 1.5 percent of antioxidant BHT, 0.5 percent of preservative sodium benzoate, 3 percent of surfactant 12-alkyl sodium sulfate, 1.5 percent of filler white carbon black, 2 percent of magnesium aluminum silicate, 0.25 percent of thickener xanthan gum, 0.2 percent of defoaming agent organic silicon and the balance of deionized water.

A Heliothis armigera nuclear polyhedrosis virus suspending agent is prepared by the following steps:

the method comprises the following steps: methyl oleate and phenethyl phenol polyoxyethylene are respectively weighed according to the weight percentage and are pre-mixed in a mixing tank.

Step two: weighing the required deionized water, 5000 hundred million PIB/gram of the mother drug of the Heliothis armigera nuclear polyhedrosis virus, antioxidant BHT, preservative sodium benzoate, surfactant 12-alkyl sodium sulfate, sodium lignosulfonate, filler white carbon black, thickener xanthan gum, magnesium aluminum silicate and defoamer organic silicon according to the weight percentage, and putting the raw materials into a mixing tank in sequence under continuous stirring to be stirred and mixed uniformly.

Step three: and starting a shearing machine to shear at a high speed, timing, and requiring that the mixing and shearing time is more than or equal to 40 minutes to fully and uniformly mix the materials.

Step four: the material is sent into a sand mill, and the sand mill and a water cooler are started (the sand milling temperature is less than or equal to 20 ℃). And (3) controlling the flow (the sanding time of each ton of the material is not less than 2.5 hours), observing the viscosity, the color and the dispersity of the sanded material, and controlling the fineness of the material (if the fineness does not reach the standard, the flow of the sanding machine can be readjusted, and the sanding time is increased until the fineness is qualified).

Step five: pumping the materials into a mixing kettle for mixing again, wherein the mixing time is more than or equal to 30 minutes.

Step six: sampling and testing. The content of the Heliothis armigera nuclear polyhedrosis virus is more than or equal to 20 hundred million PIB/miL; the unit of biological activity is more than or equal to 5000Ha U/microliter; the light stability of NPV is more than or equal to 30.0 percent; the suspension rate is more than or equal to 85 percent; the dumping property is qualified; the pH value is 6.0-7.0; the screen analysis (passing through a standard screen with the aperture of 45 microns) is more than or equal to 95 percent; the heat storage stability is qualified. All technical indexes meet the requirements and are qualified products. And if not, repeating the third step, the fourth step and the fifth step.

Step seven: packaging, and warehousing after passing the spot check.

Compared with the prior art, the cotton bollworm nuclear polyhedrosis virus suspending agent provided by the invention has the following beneficial effects:

1. the cotton bollworm nuclear polyhedrosis virus suspending agent provided by the invention contains a novel virus insecticide of cotton bollworm nuclear polyhedrosis virus, and has unique insecticidal action.

a. The pesticide is sprayed on cotton leaf surfaces, pests infect NVP through feeding, under the action of intestinal juice of the pests, inclusion body (PIB) protein is disintegrated, virus particles with infection capacity are released, the virus particles invade midgut epithelial cells to enter hemolymph, and are propagated in tissues such as trachea basement membranes, fat bodies and the like, so that systemic cells of the pests are gradually infected, and the systemic tissues of the pests are pustulated to cause death.

b. The pesticide is sprayed on the cotton bollworm, and because the pesticide has good affinity with the body wall of the pest, the pesticide quickly enters the pest body to gradually infect cells of the whole body of the pest body, and the pus of the tissue of the whole body causes death.

2. The suspending agent is added with a surfactant, an antioxidant, a preservative and the like, so that the physical and chemical properties of the preparation are effectively improved, virus particles are protected, the shelf life is prolonged, and the requirement of agricultural production on pest control is met.

3. The cotton bollworm nuclear polyhedrosis virus suspending agent provided by the invention contains more than or equal to 20 hundred million PIB/miL of cotton bollworm nuclear polyhedrosis virus; the unit of biological activity is more than or equal to 5000Ha U/microliter; the light stability of NPV is more than or equal to 30.0 percent; therefore, the composition has the advantages of high virus particle content, small dosage, good prevention and treatment effect on cotton bollworm, simple preparation process, low cost and convenient popularization and application.

Drawings

FIG. 1 is a flow chart of the preparation process of a Heliothis armigera nuclear polyhedrosis virus suspending agent.

Note in the figure that # is the quality key control point and # is the key process.

Detailed Description

To further illustrate the technical means and effects of the present invention, the following detailed description is given with reference to the preferred embodiments of the present invention and the accompanying drawings.

The cotton bollworm nuclear polyhedrosis virus suspending agent comprises the following raw materials in percentage by weight: 0.42 percent of 5000 hundred million PIB/gram of cotton bollworm nuclear polyhedrosis virus mother drug, 10 percent of methyl oleate, 6 percent of phenethylphenol polyoxyethylene ether, 1.5 percent of antioxidant BHT, 0.5 percent of preservative sodium benzoate, 3 percent of 12-alkyl sodium sulfate, 2 percent of sodium lignosulfonate, 1.5 percent of white carbon black, 2 percent of magnesium aluminum silicate, 0.25 percent of xanthan gum, 0.2 percent of organic silicon defoamer and the balance of deionized water.

Taking 1 ton of the cotton bollworm nuclear polyhedrosis virus suspending agent as an example, the preparation process is shown in figure 1, and the specific steps are as follows:

the method comprises the following steps: 100kg of methyl oleate and 60g of phenethylphenol polyoxyethylene are weighed respectively in a mixing tank and mixed uniformly (until the solution is transparent).

Step two: 726.3kg of deionized water, 4.2kg of 5000 hundred million PIB/g of cotton bollworm nuclear polyhedrosis virus mother drug, 15kg of antioxidant BHT, 5kg of preservative sodium benzoate, 30kg of surfactant 12-alkyl sodium sulfate, 20kg of sodium lignosulfonate, 15kg of filler white carbon black, 20kg of thickener magnesium aluminum silicate and 2.5kg of xanthan gum and 2.0kg of defoamer organic are respectively weighed, and the raw materials are sequentially put into a mixing tank under continuous stirring and are uniformly stirred and mixed.

Step three: and starting a shearing machine to carry out high-speed shearing and timing, wherein the mixing and shearing time is required to be more than or equal to 40 minutes, so that the materials are fully and uniformly mixed.

Step four: the material is sent into a sand mill, and the sand mill and a water cooler are started (the sand milling temperature is less than or equal to 20 ℃). And (3) controlling the flow (the sanding time of each ton of the material is not less than 2.5 hours), observing the viscosity, the color and the dispersity of the sanded material, and controlling the fineness of the material (if the fineness does not reach the standard, the flow of the sanding machine can be readjusted, and the sanding time is increased until the fineness is qualified).

Step five: pumping the materials into a mixing kettle for remixing for more than or equal to 30 minutes.

Step six: sampling and testing. All technical indexes meet the requirements and are qualified products. And grinding the unqualified product again.

Step seven: and packaging, and warehousing after the vacuum-packaged product is qualified through sampling inspection.

The cotton bollworm nuclear polyhedrosis virus suspending agent provided by the invention is packaged in vacuum, the stability of storage at normal temperature is good, and the virus activity is not obviously reduced in the storage process.

The cotton bollworm nuclear polyhedrosis virus suspending agent provided by the invention has toxicological related test results, and the following table 1 shows:

TABLE 1 Heliothis armigera nuclear polyhedrosis virus suspension toxicology test results

The test results of the cotton bollworm nuclear polyhedrosis virus suspending agent provided by the invention on the environmental impact are shown in Table 2:

TABLE 2 Heliothis armigera nuclear polyhedrosis virus suspension agent environmental impact-related test results

In order to clarify the control effect of the cotton bollworm nuclear polyhedrosis virus suspending agent provided by the invention on cotton bollworms and provide a basis for popularization and application of the cotton bollworm nuclear polyhedrosis virus suspending agent, the following tests are carried out, and the following tests are shown in table 3:

TABLE 3 Cotton bollworm prevention and control Effect of Cotton bollworm Nuclear polyhedrosis Virus suspension agent

From the test results, the cotton bollworm nuclear polyhedrosis virus suspending agent provided by the invention is low in toxicity, non-toxic to human and livestock, free of phytotoxicity, harmless to natural enemies and environment, good in effect of preventing and controlling cotton bollworms in various places, and stable in test effect. The cotton bollworm can not generate resistance after long-term use.

The above description is only for the specific embodiment of the present invention, but the protection scope of the present invention is not limited thereto, and other modifications or equivalent substitutions made by the technical solution of the present invention by the ordinary skilled in the art should be covered within the scope of the claims of the present invention without departing from the spirit and scope of the technical solution of the present invention.