阅读说明：本技术 一种用于肠菌微生物的检测试剂盒及其检测方法 (Detection kit for enterobacter microorganisms and detection method thereof ) 是由 罗海燕 于 2020-05-14 设计创作，主要内容包括：本发明公开的属于肠菌检测试剂盒技术领域,具体为一种用于肠菌微生物的检测试剂盒,包括盒体和盒盖,所述盒盖通过合页连接在盒体的上表面上,所述盒体内均匀设置有单元检测试剂盒和标签,所述单元检测试剂盒和标签之间一一对应,该用于肠菌微生物的检测试剂盒的检测方法的具体步骤如下：S1：根据需要检测的肠菌种类,在对应的标签处取下对应的单元检测试剂盒；S2：将需要检测的肠菌RNA通过加注口并贯穿橡胶塞注入到试剂盒身内；S3：通过控制开关闭合供电电池为照明灯的供电电路,照明灯通电后点亮,通过标签、单元检测试剂盒之间一一对应的配合作用,能够快速找到所需要的单元检测试剂盒,减少外界细菌的污染,保证检测的准确度。(The invention discloses a detection kit for enterobacter microorganisms, which belongs to the technical field of enterobacter detection kits, and specifically relates to a detection kit for enterobacter microorganisms, which comprises a box body and a box cover, wherein the box cover is connected to the upper surface of the box body through a hinge, unit detection kits and labels are uniformly arranged in the box body, the unit detection kits and the labels are in one-to-one correspondence, and the detection method for the detection kit for the enterobacter microorganisms comprises the following specific steps: s1: taking down the corresponding unit detection kit at the corresponding label according to the type of the intestinal bacteria to be detected; s2: injecting the intestinal bacterium RNA to be detected into the reagent box body through the filling port and the rubber plug; s3: the power supply circuit of light is closed to supply battery through control switch, lights after the light circular telegram, through the mating reaction of one-to-one between label, the unit detect reagent box, can find required unit detect reagent box fast, reduces the pollution of external bacterium, guarantees the degree of accuracy that detects.)

1. The utility model provides a detect reagent box for enterobacter microorganism, includes box body (1) and lid (4), its characterized in that: the box cover (4) is connected to the upper surface of the box body (1) through a hinge, unit detection kits (3) and labels (2) are uniformly arranged in the box body (1), and the unit detection kits (3) correspond to the labels (2) one by one;

unit detect reagent box (3) include top cap (32) and reagent box body (31), top cap (32) spiro union is at the upside of reagent box body (31), top one side of top cap (32) is provided with filling opening (34), circular logical groove has been seted up at the body middle part of top cap (32), and the embedding of circular logical inslot has perspective mirror (33), the inside downside embedding of filling opening (34) has rubber buffer (35), gene detect reagent is deposited to the inside of reagent box body (31).

2. The detection kit for an enterobacteria microorganism according to claim 1, characterized in that: the upper surface of box body (1) is provided with the draw-in groove, lid (4) joint is in the draw-in groove.

3. The detection kit for an enterobacteria microorganism according to claim 1, characterized in that: an external thread ring (312) is arranged on the outer wall of the reagent box body (31), and an internal thread hole matched with the external thread ring (312) is formed in the corresponding position in the box body (1).

4. The detection kit for an enterobacteria microorganism according to claim 1, characterized in that: the reagent box body (31) is made of transparent materials.

5. The detection kit for an enteric bacterium microorganism according to claim 4, characterized in that: the bottom of reagent box body (31) is provided with holds chamber (36), the inside that holds chamber (36) is provided with light (37), the bottom that holds chamber (36) is provided with bottom suspension fagging (38), be provided with on bottom suspension fagging (38) power supply battery (39) and control switch (310), electric connection between light (37), power supply battery (39) and the control switch (310), the bottom of bottom suspension fagging (38) is connected with supporting spring (311).

6. The detection kit for an enterobacteria microorganism according to claim 1, characterized in that: the bottom of filling port (34) is provided with the conical plate, and the conical plate is cavity hourglass hopper-shaped, conical plate and filling port (34) coaxial setting.

7. A method of detecting the detection kit for enteric bacteria microorganism as set forth in any one of claims 1 to 6, characterized in that: the detection method of the detection kit for the enterobacteria microorganisms comprises the following specific steps:

s1: according to the type of the intestinal bacteria to be detected, the corresponding unit detection kit (3) is taken down from the corresponding label (2), and the unit detection kit (3) is taken down from the box body (1) in a spiral mode;

s2: injecting the intestinal bacterium RNA to be detected into the reagent box body (31) through the filling port (34) and the rubber plug (35) so that the gene detection reagent in the reagent box body (31) can perform detection reaction on the intestinal bacterium RNA;

s3: the power supply circuit of the illuminating lamp (37) is closed by the control switch (310), the illuminating lamp (37) is lightened after being electrified, and an experimenter observes the reaction of the transparent mirror (33) in the reagent box body (31) to judge the detection result.

Technical Field

The invention relates to the technical field of an enterobacteria detection kit, in particular to a detection kit for enterobacteria microorganisms and a detection method thereof.

Background

Due to the change of living habits, the incidence of intestinal diseases of China is increased year by year, the earlier intestinal diseases are discovered, the easier the intestinal diseases are to be treated, and the early diagnosis is very important and urgent. The existing invasive examination requires complete and thorough intestinal tract preparation, and has inconvenience of privacy exposure, sedation and the like, so that the invasive examination is difficult to be widely accepted by people. With the progress of medicine, the detection by adopting a gene kit is more and more extensive.

The intestinal microorganisms refer to a large number of microorganisms existing in the intestinal tract of animals, and the group of microorganisms depends on the intestinal life of the animals and simultaneously helps the hosts to complete various physiological and biochemical functions.Intestinal tractNot only is it an important site for human digestion and absorption, but it is also the largest immune organ and plays an extremely important role in maintaining normal immune defense functions. The human intestinal tract provides a good inhabitation environment for microorganisms and has a metabolism function which is not possessed by the human body.

As the most huge and complex microecosystem of human body, the intestinal microorganisms and the metabolites thereof can not only regulate the health of human body, but also play an important bridge role between diet and host.

The detection kit is used for containing a box for detecting chemical reagents such as chemical components, drug residues, virus types and the like. Is generally used by hospitals and pharmaceutical enterprises.

When the existing detection kit is used, the storage, observation and taking out of a detection reagent are not convenient enough.

Disclosure of Invention

The invention aims to provide a detection kit for enterobacter microorganisms and a detection method thereof, and aims to solve the problem that the existing detection kit proposed in the background technology is inconvenient to store, observe and take out detection reagents when in use.

In order to achieve the purpose, the invention provides the following technical scheme: a detection kit for enterobacter microorganisms comprises a box body and a box cover, wherein the box cover is connected to the upper surface of the box body through hinges, unit detection kits and labels are uniformly arranged in the box body, and the unit detection kits and the labels are in one-to-one correspondence;

unit detect reagent box includes top cap and reagent box body, the top cap spiro union is at the upside of reagent box body, top one side of top cap is provided with the filler, circular logical groove has been seted up at the body middle part of top cap, and the embedding of circular logical inslot has perspective mirror, the inside downside embedding of filler has the rubber buffer, gene detect reagent is deposited to the inside of reagent box body.

Preferably, the upper surface of the box body is provided with a clamping groove, and the box cover is clamped in the clamping groove.

Preferably, the outer wall of the reagent box body is provided with an external thread ring, and an internal thread hole matched with the external thread ring is formed in the corresponding position inside the box body.

Preferably, the kit body is a transparent kit body.

Preferably, the bottom of reagent box body is provided with and holds the chamber, the inside that holds the chamber is provided with the light, the bottom that holds the chamber is provided with the bottom suspension fagging, be provided with power supply battery and control switch in the bottom suspension fagging, electric connection between light, power supply battery and the control switch, the bottom of bottom suspension fagging is connected with supporting spring.

Preferably, the bottom of the filling opening is provided with a conical plate, the conical plate is hollow and funnel-shaped, and the conical plate and the filling opening are coaxially arranged.

The detection method of the detection kit for the enterobacteria microorganisms comprises the following specific steps:

s1: according to the type of the intestinal bacteria to be detected, taking down the corresponding unit detection kit at the corresponding label, wherein the unit detection kit is taken down from the box body in a spiral mode;

s2: injecting the enterobacteria RNA to be detected into the reagent box body through the filling port and the rubber plug so that the gene detection reagent in the reagent box body reacts on the detection of the enterobacteria RNA;

s3: the power supply circuit of the illuminating lamp is closed by the control switch, the illuminating lamp is lightened after being electrified, and an experimenter observes the reaction in the reagent box body through the perspective lens to judge a detection result.

Compared with the prior art, the invention has the beneficial effects that:

1) through the arrangement of the filling port, the steps of opening and closing the reagent box body are reduced, the pollution of external bacteria is reduced, and the detection accuracy is ensured;

2) through the arrangement of the rubber plug and the conical plate, the reagent in the device can be prevented from flowing out;

3) through the one-to-one matching action between the label and the unit detection kit, the required unit detection kit can be quickly found, and the efficiency is improved.

Drawings

FIG. 1 is a schematic structural view of the present invention;

FIG. 2 is a schematic top view of the case according to the present invention;

FIG. 3 is a schematic view of the structure of the unit detection kit of the present invention;

FIG. 4 is a schematic sectional view of the unit detection kit of the present invention.

In the figure: the kit comprises a box body 1, a label 2, a 3-unit detection kit, a 31 reagent box body, a 32 top cover, a 33 perspective mirror, a 34 filling port, a 35 rubber plug, a 36 accommodating cavity, a 37 illuminating lamp, a 38 lower supporting plate, a 39 power supply battery, a 310 control switch, a 311 supporting spring, a 312 external thread ring and a 4 box cover.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

In the description of the present invention, it is to be understood that the terms "upper", "lower", "front", "rear", "left", "right", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, are merely for convenience in describing the present invention and simplifying the description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention.