阅读说明：本技术 一种从生物碱浸膏中分离纯化山莨菪碱单体的方法 (Method for separating and purifying anisodamine monomer from alkaloid leaching paste ) 是由 于喜洋 刘千驰 刘东滨 于 2021-08-31 设计创作，主要内容包括：本发明提出了一种从生物碱浸膏中分离纯化山莨菪碱单体的方法,涉及化工生产技术领域。该从生物碱浸膏中分离纯化山莨菪碱单体的方法包括如下步骤：将生物碱浸膏粉碎过筛,得到浸膏颗粒,将浸膏颗粒用酸液酸化,得到盐溶液,将盐溶液用碱液碱化后洗脱,得到生物碱溶液,向生物碱溶液中加入复合酶反应后得到酶解液,将酶解液离心后过滤上层溶液,即得到山莨菪碱单体；此方法不仅能够有效提升分离纯化所得到的山莨菪碱单体的产率,且还能够有效提高所分离纯化出的山莨菪碱单体的纯度。(The invention provides a method for separating and purifying anisodamine monomers from alkaloid leaching paste, and relates to the technical field of chemical production. The method for separating and purifying the anisodamine monomer from the alkaloid leaching paste comprises the following steps: crushing and sieving the alkaloid extract to obtain extract particles, acidifying the extract particles with acid liquor to obtain salt solution, alkalifying the salt solution with alkali liquor and then eluting to obtain alkaloid solution, adding complex enzyme into the alkaloid solution for reaction to obtain enzymatic hydrolysate, centrifuging the enzymatic hydrolysate and filtering the upper-layer solution to obtain anisodamine monomers; the method not only can effectively improve the yield of the separated and purified anisodamine monomer, but also can effectively improve the purity of the separated and purified anisodamine monomer.)

1. A method for separating and purifying anisodamine monomers from alkaloid extract is characterized by comprising the following steps of crushing and sieving the alkaloid extract containing anisodamine to obtain extract particles, acidifying the extract particles by using acid liquor to obtain salt solution, alkalifying the salt solution by using alkali liquor and then eluting to obtain alkaloid solution, adding complex enzyme into the alkaloid solution to react to obtain enzymatic hydrolysate, centrifuging the enzymatic hydrolysate and filtering the upper-layer solution to obtain the anisodamine monomers.

2. The method for separating and purifying anisodamine monomers from alkaloid leachate of claim 1, wherein the acid solution is sulfuric acid, and the concentration of sulfuric acid is 15-25%.

3. The method for separating and purifying anisodamine monomers from alkaloid leachate of claim 1, wherein the alkaline solution is ammonia.

4. The method for separating and purifying anisodamine monomers from alkaloid leachate of claim 1, wherein the pH after alkalization is 9-10.

5. The method for separating and purifying anisodamine monomers from alkaloid leaching paste according to claim 1, wherein the elution is gradient elution and the mobile phase used in the elution is ammonia-methanol.

6. The method for separating and purifying anisodamine monomers from alkaloid leachate of claim 1, wherein the complex enzyme comprises one or more of cellulase, protease, pectinase and xylanase.

7. The method for separating and purifying anisodamine monomers from alkaloid leachate of claim 6, wherein the enzymatic activities of said cellulase, protease, pectinase and xylanase are all 0.2-2.0U/g.

8. The method for separating and purifying anisodamine monomers from alkaloid leaching paste as claimed in claim 1, wherein the filtration step further comprises a drying step, wherein the drying step comprises a step of obtaining a precipitate after the filtration step, and heating the precipitate at 100-110 ℃ for 20-30 min.

9. The method for separating and purifying anisodamine monomer from alkaloid leaching paste as claimed in claim 1, wherein the rotation speed of the centrifugation is 3000-8000r/min, and the time of the centrifugation is 15-30 min.

10. The method for separating and purifying anisodamine monomers from alkaloid leaching paste according to any one of claims 1-9, wherein the preparation method of the alkaloid leaching paste comprises the following steps of grinding, alcohol extracting and filtering the radix physochlainae in sequence to obtain filtrate, extracting the filtrate with chloroform, and then concentrating under reduced pressure to obtain the alkaloid leaching paste.

Technical Field

The invention relates to the technical field of chemical production, and particularly relates to a method for separating and purifying anisodamine monomers from alkaloid leaching paste.

Background

Anisodamine, commonly used as its hydrobromide, is a white crystal or crystalline powder, odorless. Is very soluble in water. It is easily soluble in ethanol. The hyoscyamine has obvious peripheral anticholinergic effect, can resist the contraction of intestinal and bladder smooth muscle and the decrease of blood pressure caused by acetylcholine, and can reduce the tension of the intestinal in vivo, and the action intensity is similar to that of atropine. Alias: 7-hydroxy hyoscyamine; racanisodamine hydrobromide; anisodamine hydrobromide. Chemical name: an alkaloid extracted from the special solanaceae plant anisodamine in China is often called as 654 for short, and the natural product of the alkaloid is 654-1. Prepared by artificial synthesis methodThe product is '654-2'. CAS number: 55869-99-3. The molecular formula is as follows: c₁₇H₂₃NO₄. Molecular weight: 305.36900.

the anisodamine monomer has higher medicinal value and is a common nerve blocking medicament, and the anisodamine monomer separated by the prior common purification method of the anisodamine monomer has more impurities, and is unstable and modified into other substances, thereby reducing the yield of the anisodamine.

Therefore, there is a need in the market to provide a method for purifying anisodamine monomer with higher yield and higher purity of the obtained product.

Disclosure of Invention

The invention aims to provide a method for separating and purifying anisodamine from alkaloid leaching paste, which not only can effectively improve the yield of the anisodamine monomer obtained by separation and purification, but also can effectively improve the purity of the separated and purified anisodamine monomer.

The technical problem to be solved by the invention is realized by adopting the following technical scheme.

The embodiment of the application provides a method for separating and purifying anisodamine from alkaloid extract, which comprises the following steps of crushing and sieving the alkaloid extract to obtain extract particles, acidifying the extract particles with acid liquor to obtain a salt solution, alkalifying the salt solution with alkali liquor and then eluting to obtain alkaloid solution, adding complex enzyme into the alkaloid solution for reaction to obtain an enzymatic hydrolysate, centrifuging the enzymatic hydrolysate and filtering the upper solution to obtain the anisodamine monomer. The method comprises the steps of firstly converting some alkaline substances, insoluble substances and the like in an alkaloid extract into salt substances through acidification of an acid solution, then converting the generated salt substances into anisodamine through an alkali solution so as to further improve the yield of the finally prepared anisodamine, and then sequentially removing other alkaloid impurities which are difficult to remove through elution, enzymolysis and centrifugation so as to further improve the purity of the finally prepared anisodamine.

Compared with the prior art, the embodiment of the invention has at least the following advantages or beneficial effects:

the embodiment of the invention provides a method for separating and purifying anisodamine monomers from alkaloid leaching paste, which comprises the steps of firstly crushing and sieving extract to remove large-particle impurities, increasing the contact area and reaction degree of subsequent extract and acid liquor, reacting anisodamine insoluble substances and other impurities as much as possible into anisodamine salt, then adding ammonia water for alkalization to improve the final yield of anisodamine, removing other residual alkaloids which are difficult to remove by elution to finally obtain the anisodamine monomers, and removing other impurities such as cell walls and the like remained in plants by enzymolysis to prevent the anisodamine monomers obtained after final centrifugation from being impure.

Detailed Description

In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. The examples, in which specific conditions are not specified, were conducted under conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used are not indicated by the manufacturer, and are all conventional products available commercially.

It should be noted that the embodiments and features of the embodiments in the present application may be combined with each other without conflict. The present invention will be described in detail below with reference to specific examples.

The embodiment of the application provides a method for separating and purifying anisodamine from alkaloid extract, which comprises the following steps of crushing and sieving the alkaloid extract to obtain extract particles, acidifying the extract particles with acid liquor to obtain a salt solution, alkalifying the salt solution with alkali liquor and then eluting to obtain alkaloid solution, adding complex enzyme into the alkaloid solution for reaction to obtain an enzymatic hydrolysate, centrifuging the enzymatic hydrolysate and filtering the upper solution to obtain the anisodamine monomer. The method comprises the steps of firstly crushing and sieving an extract to remove large-particle impurities, increasing the contact area and the reaction degree of a subsequent extract and an acid solution, reacting more anisodamine insoluble substances and other impurities as much as possible to obtain anisodamine salt, then alkalizing the anisodamine by adding ammonia water to generate more anisodamine so as to improve the yield of the final anisodamine, removing other residual alkaloids which are difficult to remove by elution to finally obtain an anisodamine monomer and improve the purity of the finally obtained anisodamine monomer, removing some impurities which are difficult to remove by elution and the like and are remained by plants during the preparation of the alkaloid extract by enzymolysis so as to prevent the anisodamine monomer obtained after final centrifugation from being impure, and finally settling the anisodamine monomer at the bottom by centrifugation, removing the upper liquid to obtain high-purity anisodamine monomer.

In some embodiments of the present invention, the acid solution is sulfuric acid, and the concentration of the sulfuric acid is 15-25%. Sulfuric acid is an inorganic compound of the formula H₂SO₄The most important oxyacid of sulfur. Pure sulfuric acid is colorless oily liquid, crystallizes at 10.36 ℃, and is usually prepared by a tower method and a contact method by using aqueous solutions of various concentrations. The former is crude dilute sulfuric acid, and the mass fraction is about 75% generally; the latter can obtain concentrated sulfuric acid with mass fraction of 98.3%, boiling point of 338 deg.C, and relative density of 1.84. The sulfuric acid has strong acidity, can react with various alkaline and neutral substances, and can also react with some weakly acidic substances, so that the finally generated salt can also react with some weakly alkaline liquids, and further the yield of the finally generated anisodamine is improved, and the utilization rate of the alkaloid extract is improved.

In some embodiments of the present invention, the alkali solution is ammonia. The ammonia water is alkalescent and is convenient to volatilize, and precipitates are not easy to form, so that the ammonia water is selected as alkali liquor to alkalize the anisodamine, the final yield of the anisodamine is improved, the ammonia gas generated in the reaction process of the ammonia water can volatilize, other precipitates are not easy to remain, and the finally prepared anisodamine monomer is impure.

In some embodiments of the invention, the pH after basification is from 9 to 10. The pH interval can ensure that the amount of the anisodamine monomer generated due to the over-low pH is not less, thereby causing the yield of the final generated anisodamine monomer to be lower. And the generated precipitate and excessive other alkaline substances caused by overhigh pH value can not be generated, so that the purity of the finally generated anisodamine monomer is lower.

In some embodiments of the present invention, the elution is a gradient elution, and the mobile phase used for elution is ammonia-methanol. Gradient elution is also known as gradient elution or program elution. In the same analysis period, the concentration ratio of the mobile phase is changed to a certain extent, which is called gradient elution. In gas chromatography, temperature-programmed methods are widely used in order to improve the separation of wide boiling range samples and to shorten the analysis period. In liquid chromatography, gradient elution is adopted for samples with complex components. In the same analysis period, the concentration ratio of the mobile phase is changed continuously according to a certain program, which is called gradient elution. Therefore, the components with larger property difference in a complex sample can achieve good separation according to the respectively proper capacity factor k. Gradient elution or isocratic elution is used, mainly in the case of impurities. When the same substance has two conditions of gradient elution and isocratic elution, the acceptance of gradient elution is higher. The degree of separation of impurities from impurities, main peaks, and solvent peaks must be good. (1) The polarity difference between the impurities and the main component is large, and the impurities can be eluted completely (sometimes, the impurities cannot be eluted after being flushed for two or three hours under the condition of equal temperature, and the use of gradient is required to be considered). (2) The polarity difference between the impurities and the main component is large, the impurity peak emergence is too late (the sample detection time can be shortened by gradient after two hours of isocratic flushing) (3) the impurity polarity is larger than that of the main peak, the proper time of the main peak is required to be fully piled in front when isocratic flushing is carried out, the impurities are separated from the main peak and the peak emergence is too late, and the gradient elution is also considered in the condition. In the invention, the peak difference between the anisodamine and other alkaloids in the alkaloid extract is larger, so that the gradient elution can ensure that the anisodamine monomer is better eluted, and the purity of the anisodamine monomer is improved. The ammonia water-methanol is mainly selected as a mobile phase for elution, and firstly, the ammonia water can create alkaline relief for elution, so that the desorption of the anisodamine monomer is facilitated, and the higher purity of the eluted anisodamine monomer is ensured. The methanol can destroy functional hydrogen bonds formed between the anisodamine and other alkaloids, and can effectively eliminate charge action generated between the anisodamine and other alkaloids so as to elute the anisodamine, and the methanol is selected as an eluent to achieve a better elution effect because of the higher polarity of the anisodamine. The weight ratio of ammonia water to methanol is preferably 1 (4-10), and the interval not only can ensure the elution speed, but also can ensure the elution effect so as to improve the purity of the finally prepared anisodamine monomer as much as possible.

In some embodiments of the invention, the complex enzyme comprises one or more of cellulase, protease, pectinase and xylanase. Cellulase (beta-1, 4-glucan-4-glucan hydrolase) is a general name of a group of enzymes for degrading cellulose to generate glucose, is not a monomer enzyme, is a multi-component enzyme system with a synergistic effect, is a complex enzyme, mainly comprises exo-beta-glucanase, endo-beta-glucanase, beta-glucosidase and the like, and also has xylanase with high activity. Acting on cellulose and products derived from cellulose. The alkaloid extract is directly prepared from plant raw materials, so that plant cell walls and other components are remained in the alkaloid extract, and cellulose and the like contained in the cell walls are easy to reduce the purity of the finally prepared anisodamine monomer, so that the anisodamine monomer is decomposed into monosaccharides and the like with small molecular weights and difficult to settle by adopting cellulase, and the condition that the anisodamine monomer is settled after centrifugation is avoided, and the purity of the anisodamine monomer is finally reduced. In addition, the main components of the cell wall also consist of pectin and xylan, so that the xylan and the pectin in the cell wall can be effectively hydrolyzed by further adding pectinase and xylanase, and the purity of the prepared anisodamine monomer is improved. Besides cellulose and the like, the alkaloid extract also contains various macromolecular proteins, so that the protease is used for hydrolyzing the various macromolecular proteins into micromolecular amino acids, thereby avoiding the protein from settling and improving the purity of the finally prepared anisodamine monomer.

In some embodiments of the invention, the enzymatic activities of the cellulase, protease, pectinase and xylanase are all 0.2-2.0U/g. The enzyme between the activity intervals can ensure that the decomposition speed of the enzyme is uniform and constant when the enzyme is decomposed, the excessively high enzyme activity can not result in the excessively high decomposition speed, and further other products can be generated, and in addition, the excessively low enzyme activity can not result in the excessively low decomposition speed, and finally the excessively long enzymolysis speed can not be caused. In the invention, the enzymolysis time of the enzyme activity in the interval is about 30-50min so as to ensure full enzymolysis.

In some embodiments of the present invention, the filtering further comprises drying, wherein the drying comprises the steps of filtering to obtain a precipitate, and heating the precipitate at 110 ℃ for 20-30 min. The drying can not only evaporate the water in the prepared anisodamine monomer to further improve the purity of the anisodamine monomer, but also can stabilize the crystal structure of the prepared anisodamine monomer and avoid the unstable anisodamine monomer from easily reacting.

In some embodiments of the present invention, the rotation speed of the centrifugation is 3000-8000r/min, and the centrifugation time is 15-30 min. The centrifugal rotation speed interval and time interval can settle the anisodamine monomer as much as possible, and simultaneously can avoid the settlement of other alkaloids or impurities, thereby improving the purity of the finally prepared anisodamine monomer.

In some embodiments of the present invention, the preparation method of the alkaloid extract comprises the following steps of sequentially grinding, extracting with ethanol and filtering the physochlaina huashanensis to obtain a filtrate, extracting the filtrate with chloroform, and concentrating under reduced pressure to obtain the alkaloid extract. The method discloses a preparation method of an alkaloid extract, wherein part of impurities which are difficult to remove can be primarily removed by extracting with chloroform. Radix Physochlainae is dry root of Physochlaina Pubescens of Solanaceae. Plucked in spring, cleaned, and dried in the sun. Distributed from the middle of Qin mountain in Shaanxi to the east, the west and south of Henan, and the south of Shanxi. Has effects of warming lung, eliminating phlegm, relieving asthma and cough, tranquilizing mind, and relieving convulsion. Can be used for treating cough and asthma due to cold phlegm, palpitation, and insomnia, and other plants containing anisodamine such as Phyllanthus niruri.

The features and properties of the present invention are described in further detail below with reference to examples.

Example 1

The embodiment provides a method for separating and purifying anisodamine from alkaloid leaching paste, which comprises the following steps of sequentially grinding, extracting and filtering radix physochlainae to obtain filtrate, extracting the filtrate with chloroform, concentrating under reduced pressure, crushing and sieving the prepared alkaloid extract to obtain extract particles, acidifying the extract particles with 15% sulfuric acid to obtain a salt solution, alkalizing the salt solution to pH 9 with ammonia water, carrying out gradient elution with ammonia water-methanol to obtain a alkaloid solution, adding cellulase with enzyme activity of 0.2U/g into the alkaloid solution to react for 30min to obtain an enzymatic hydrolysate, centrifuging the enzymatic hydrolysate for 15min at a rotating speed of 3000r/min, filtering the upper-layer solution to obtain a precipitate, and heating the precipitate at 100 ℃ for 20min to obtain the anisodamine monomer.

Example 2

The embodiment provides a method for separating and purifying anisodamine from alkaloid leaching paste, which comprises the following steps of grinding, alcohol extracting and filtering radix physochlainae in sequence to obtain filtrate, extracting the filtrate with chloroform, concentrating under reduced pressure, crushing and sieving the prepared alkaloid extract to obtain extract particles, acidifying the extract particles with 25% sulfuric acid to obtain a salt solution, alkalizing the salt solution to pH 10 with ammonia water, and then carrying out gradient elution with ammonia water-methanol to obtain a alkaloid solution, adding cellulase, pectinase, xylanase and protease with the enzyme activity of 2.0U/g into the alkaloid solution to react for 50min to obtain an enzymatic hydrolysate, centrifuging the enzymatic hydrolysate for 30min at the rotating speed of 8000r/min, filtering the upper solution to obtain a precipitate, and heating the precipitate for 30min at the temperature of 110 ℃ to obtain the anisodamine monomer.

Example 3

The embodiment provides a method for separating and purifying anisodamine from alkaloid leaching paste, which comprises the following steps of grinding, alcohol extracting and filtering radix physochlainae in sequence to obtain filtrate, extracting the filtrate with chloroform, then carrying out reduced pressure concentration, crushing and sieving the prepared alkaloid extract to obtain extract particles, acidifying the extract particles with 20% sulfuric acid to obtain a salt solution, alkalizing the salt solution to pH 9.5 with ammonia water, carrying out gradient elution with ammonia water-methanol to obtain a alkaloid solution, adding cellulase, pectinase, xylanase and protease with the enzyme activity of 1.1U/g into the alkaloid solution to react for 40min to obtain an enzymatic hydrolysate, centrifuging the enzymatic hydrolysate for 23min at the rotating speed of 5500r/min, filtering the upper-layer solution to obtain a precipitate, and heating the precipitate at 105 ℃ for 25min to obtain the anisodamine monomer.

Example 4

The embodiment provides a method for separating and purifying anisodamine from alkaloid leaching paste, which comprises the following steps of sequentially grinding, extracting and filtering radix physochlainae to obtain filtrate, extracting the filtrate with chloroform, concentrating under reduced pressure, crushing and sieving the prepared alkaloid extract to obtain extract particles, acidifying the extract particles with 18% sulfuric acid to obtain a salt solution, alkalizing the salt solution to pH 9.2 with ammonia water, carrying out gradient elution with ammonia water-methanol to obtain a alkaloid solution, adding cellulase with enzyme activity of 1.0U/g and protease into the alkaloid solution to react for 37min to obtain an enzymatic hydrolysate, centrifuging the enzymatic hydrolysate for 18min at the rotating speed of 5000r/min, filtering the upper-layer solution to obtain a precipitate, and heating the precipitate at 102 ℃ for 22min to obtain the anisodamine monomer.

Example 5

The embodiment provides a method for separating and purifying anisodamine from alkaloid leaching paste, which comprises the following steps of grinding, alcohol extracting and filtering radix physochlainae in sequence to obtain filtrate, extracting the filtrate with chloroform, then carrying out reduced pressure concentration, crushing and sieving the prepared alkaloid extract to obtain extract particles, acidifying the extract particles with 23% sulfuric acid to obtain a salt solution, alkalizing the salt solution to pH 9.7 with ammonia water, carrying out gradient elution with ammonia water-methanol to obtain a alkaloid solution, adding cellulase, xylanase, pectinase and protease with the enzyme activity of 1.3U/g into the alkaloid solution to react for 44min to obtain an enzymatic hydrolysate, centrifuging the enzymatic hydrolysate for 28min at the rotating speed of 6000r/min, filtering the upper layer solution to obtain a precipitate, and heating the precipitate at 108 ℃ for 27min to obtain the anisodamine monomer.

Example 6

The embodiment provides a method for separating and purifying anisodamine from alkaloid leaching paste, which comprises the following steps of grinding, alcohol extracting and filtering radix physochlainae in sequence to obtain filtrate, extracting the filtrate with chloroform, then carrying out reduced pressure concentration, crushing and sieving the prepared alkaloid extract to obtain extract particles, acidifying the extract particles with 16% sulfuric acid to obtain a salt solution, alkalizing the salt solution to pH 9.9 with ammonia water, carrying out gradient elution with ammonia water-methanol to obtain a alkaloid solution, adding cellulase, pectinase and xylanase with enzyme activity of 0.7U/g into the alkaloid solution to react for 37min to obtain an enzymatic hydrolysate, centrifuging the enzymatic hydrolysate for 21min at a rotating speed of 4800r/min, filtering the upper layer solution to obtain a precipitate, and heating the precipitate at 109 ℃ for 29min to obtain the anisodamine monomer.

Test example 1

Taking the anisodamine monomer prepared in the examples 1-6 and weighing (g), taking the same root of 50g of scopolamine to prepare alkaloid extract and the anisodamine monomer by adopting the conventional separation and purification process on the market at present and weighing (g), taking the group as a comparative example, and then according to the formula: the yield (%) of the anisodamine monomer was calculated by multiplying the weight of the anisodamine monomer/the weight of the alkaloid extract by 100%, and the results are shown in table 1.

TABLE 1 anisodamine monomer yield table

As can be seen from the results in table 1, the yield of the anisodamine monomer prepared in the examples 1-6 of the present invention is slightly higher than that of the anisodamine monomer prepared in the prior art, and thus it can be seen that the production process of the anisodamine monomer provided by the present invention can improve the yield of the anisodamine monomer.

Test example 2

Taking the anisodamine monomers prepared in examples 1-6 and sequentially detecting the components and contents of anisodamine by chromatography, wherein the anisodamine monomer prepared in example 1 contains two lower peaks in addition to the peak of anisodamine, wherein the anisodamine monomer prepared in example 2 contains one lower peak and one higher peak in addition to the peak of anisodamine, wherein the anisodamine monomer prepared in example 3 contains one lower peak in addition to the peak of anisodamine, wherein the anisodamine monomer prepared in example 4 contains one lower peak in addition to the peak of anisodamine, wherein the anisodamine monomer prepared in example 5 contains one higher peak in addition to the peak of anisodamine, wherein the anisodamine monomer prepared in example 6 contains one higher peak and one lower peak in addition to the peak of anisodamine .

And (3) taking the anisodamine monomer prepared in the comparative example, and detecting the components and the content of the anisodamine monomer by adopting a chromatography, wherein the anisodamine monomer prepared in the comparative example contains two lower peaks and two higher peaks besides the anisodamine peak.

According to the results, the peak value of the chromatogram of the anisodamine monomer obtained in the embodiment of the invention is less than that of the chromatogram of the anisodamine monomer obtained in the comparative example, so that the impurity content of the anisodamine monomer obtained in the embodiment of the invention is obviously lower than that of the anisodamine monomer obtained in the comparative example, and therefore, the conclusion can be drawn that the purity of the anisodamine monomer obtained by the production process of the anisodamine monomer provided by the invention is obviously higher than that of the existing preparation process on the market.

In summary, embodiments of the present invention provide a method for separating and purifying anisodamine monomers from alkaloid leaching paste, the method comprises the steps of crushing and sieving an extract to remove large-particle impurities, increasing the contact area and reaction degree of a subsequent extract and an acid solution, reacting an amount of anisodamine insoluble substances and other impurities as much as possible to obtain anisodamine salt, adding ammonia water for alkalization to improve the final yield of anisodamine, eluting to remove other residual alkaloids which are difficult to remove, to obtain the anisodamine monomers, and performing enzymolysis to remove other impurities such as cell walls remaining in plants, so as to prevent the anisodamine monomers obtained after final centrifugation from being impure.

The embodiments described above are some, but not all embodiments of the invention. The detailed description of the embodiments of the present invention is not intended to limit the scope of the invention as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.