阅读说明：本技术 一种甲氨基阿维菌素苯甲酸盐纳米缓释农药制剂及其制备方法 (Methylamino abamectin benzoate nano slow-release pesticide preparation and preparation method thereof ) 是由 沈健 宋赛杰 冯文立 周宁琳 王玉丽 于 2020-06-01 设计创作，主要内容包括：本发明公开了一种甲氨基阿维菌素苯甲酸盐纳米缓释农药制剂及其制备方法。取适量的石墨烯氧化物粉末于蒸馏水中并超声分散,再加入一定量的甲氨基阿维菌素苯甲酸盐及绿色环保型三元共聚物助剂,形成均匀分散的甲氨基阿维菌素苯甲酸盐纳米缓释农药。与传统农药制剂制备方法相比,本发明所述农药制剂具有工艺简单、操作方便、绿色环保、可控缓释等特点。通过缓释实验测试,该农药制剂具有缓释性能。本发明为农药的减施增效及农业可持续发展提供新的思路。(The invention discloses an emamectin benzoate nano slow-release pesticide preparation and a preparation method thereof. Taking a proper amount of graphene oxide powder in distilled water, performing ultrasonic dispersion, and adding a certain amount of emamectin benzoate and a green environment-friendly terpolymer auxiliary agent to form the uniformly dispersed emamectin benzoate nano slow-release pesticide. Compared with the traditional preparation method of the pesticide preparation, the pesticide preparation has the characteristics of simple process, convenient operation, environmental protection, controllable slow release and the like. Through slow release experiment tests, the pesticide preparation has slow release performance. The invention provides a new idea for the reduction of the application and the improvement of the pesticide and the sustainable development of agriculture.)

1. A preparation method of a methylamino abamectin benzoate nano slow-release pesticide preparation is characterized by comprising the following steps: dissolving graphene oxide in distilled water, wherein the graphene oxide is one or a mixture of more of graphene oxide, reduced graphene oxide, sulfonated graphene oxide, aminated graphene oxide and alkylated graphene oxide; then, the emamectin benzoate and the green environment-friendly type multi-component compound auxiliary agent are added and evenly mixed by a high-speed homogenizer to obtain the emamectin benzoate nano slow-release pesticide preparation which is stably dispersed in the water phase.

2. The preparation method of the emamectin benzoate nano slow-release pesticide preparation according to claim 1, which is characterized by comprising the following steps of: the green environment-friendly type multi-component compound auxiliary agent comprises: phosphorus-containing terpolymer NDF-04, polycarboxy terpolymer, dimethyl-monoperfluorodecylsebacic silicon-polyethoxy-oleate polybasic compound, dimethyl-monoperfluorodecylsebacic silicon-polyethoxy-stearate polybasic compound, methyl-diperfluorodecylsebacic silicon-polyethoxy-sodium benzenesulfonate polybasic compound, methyl-diperfluorodecylsebacic silicon-polyethoxy-stearate polybasic compound, triperfluorodecylsilylpolyethoxy-sodium naphthalenesulfonate polybasic compound, methyl-diperfluorodecylsebacic silicon-polyethoxy-oleate polybasic compound, triperfluorodecylsilyl-polyethoxy-stearate polybasic compound, triperfluorodecylsilyl-polyethoxy-sodium benzenesulfonate polybasic compound, poly (ethylene glycol) methyl-perfluorodecyl silicon-polyethoxy-oleate, poly (ethylene glycol) ethyl acrylate, poly (, One or more of methyl-bis-perfluorodecyl silicon-polyethoxy-sodium naphthalene sulfonate polybasic compound, dimethyl-mono-perfluorodecyl silicon-polyethoxy-sodium benzene sulfonate polybasic compound, dimethyl-mono-perfluorodecyl silicon-polyethoxy-sodium naphthalene sulfonate polybasic compound and tri-perfluorodecyl silicon-polyethoxy-oleate polybasic compound.

3. The preparation method of the emamectin benzoate nano slow-release pesticide preparation according to claim 1, which is characterized by comprising the following steps of: the content of graphene oxide in the emamectin benzoate nano slow-release pesticide preparation is 0.1-5%, the content of emamectin benzoate is 0.1-5%, and the content of the green environment-friendly terpolymer auxiliary agent is 0.5-5%.

4. The preparation method of the emamectin benzoate nano slow-release pesticide preparation according to claim 3, which is characterized by comprising the following steps of: the content of graphene oxide in the emamectin benzoate nano slow-release pesticide preparation is 0.5-2%, the content of emamectin benzoate is 0.8-2.5%, and the content of the green environment-friendly terpolymer auxiliary agent is 0.5-2%.

5. The preparation method of the emamectin benzoate nano slow-release pesticide preparation according to claim 1, which is characterized by comprising the following steps of: the rotating speed of the high-speed homogenizing machine is 1-20 k revolutions per minute, and the time for uniformly mixing is 5-120 minutes.

6. The preparation method of the emamectin benzoate nano slow-release pesticide preparation according to claim 5, which is characterized by comprising the following steps of: the rotating speed of the high-speed homogenizing machine is 5-15 k revolutions per minute, and the time for uniformly mixing is 15-60 minutes.

7. The emamectin benzoate nano slow-release pesticide preparation prepared by the method according to any one of claims 1 to 6.

8. The application of the emamectin benzoate nano slow-release pesticide preparation prepared by the method according to claim 7 in preventing and treating armyworm.

Technical Field

The invention relates to the technical field of plant protection, in particular to an emamectin benzoate nano slow-release pesticide preparation and a preparation method thereof.

Background

Graphene is a novel two-dimensional carbon nanomaterial, and has been widely researched and applied in the fields of electronic devices, computer elements, energy chemical industry, environmental protection, biomedicine and the like. Graphene oxide is the most important derivative in graphene family materials, has the characteristics of large specific surface area, good water solubility, easy-to-modify surface chemical structure, ultrahigh adsorption performance and the like, is a good carrier, and is widely applied to the delivery field of biological medicines. For example, the topic group of professor dynode in 2008 firstly reports that nano graphene oxide is used for loading hydrophobic chemotherapeutic drug SN38 to construct an SN38 nano drug delivery system with pH value response. After that, due to its simple preparation process, good biocompatibility, and ultra-high drug-loading performance, the research of delivering hydrophobic drugs with graphene oxide as a carrier is receiving much attention. Researches indicate that the drug loading rate of the graphene oxide to chemotherapy drug adriamycin can reach as high as 400%. The research of graphene materials in the field of biomedicine has been widely based, but the research has not been reported in a large number and systems in the field of pesticide delivery. Research shows that the drug loading rate of the graphene oxide-based pesticide delivery system is between 5 and 60 percent at home and abroad at present. Therefore, the graphene drug delivery system is popularized to the field of pesticide delivery, and has great significance for controllable drug delivery and reduced application and synergy of pesticides.

Emamectin benzoate (hereinafter abbreviated as emamectin benzoate) is a low-toxicity and high-efficiency biopesticide, has stomach toxicity and contact poisoning effects, has good killing effect on pests such as mites, lepidoptera, coleoptera and the like, and has important application value in pest control of economic crops such as vegetables, tobacco, fruit trees, cotton, tea leaves and the like. At present, emamectin benzoate mainly takes missible oil, microemulsion, wettable powder, granules and the like as main preparations, and has the defects of low stability, easy burst release, poor persistence and the like.

Therefore, the graphene drug delivery system is combined with emamectin benzoate by utilizing the nano materials and the nano technology, so that the pesticide which does not relate to toxic organic solvents and auxiliaries and has high drug-loading performance is developed, the green production of pesticide preparations is promoted, and the national requirements on zero increase, reduced application and synergistic effect of the pesticide are met.

Disclosure of Invention

The purpose of the invention is as follows: the invention provides a preparation method of an emamectin benzoate nano slow-release pesticide preparation with simple process and environmental protection. Graphene oxide is used as a carrier, a green environment-friendly terpolymer is used as an auxiliary agent, and the emamectin benzoate preparation with good water dispersibility, stability and slow release is obtained through simple physical blending. The invention provides a new idea for the cross-border application of graphene, green production of pesticides and sustainable development.

The technical scheme is as follows: the invention provides a preparation method of a methylamino abamectin benzoate nano slow-release pesticide preparation, which is characterized in that graphene oxide is dissolved in distilled water, wherein the graphene oxide is one or a mixture of more of graphene oxide, reduced graphene oxide, sulfonated graphene oxide, aminated graphene oxide and alkylated graphene oxide; then, the emamectin benzoate and the green environment-friendly type multi-component compound auxiliary agent are added and evenly mixed by a high-speed homogenizer to obtain the emamectin benzoate nano slow-release pesticide preparation which is stably dispersed in the water phase. The high-speed homogenizer can cut the solid in the suspension solution to a nano size, and the carrier is fully contacted with the pesticide under the action of high-speed stirring and cutting, so that the interaction between the carrier and the pesticide is enhanced, and the adsorption efficiency is improved. Meanwhile, the green environment-friendly multi-component compound additive can also play a role of a stabilizer, and prevents hydrophobic pesticide from being separated out and precipitated due to aggregation, so that the pesticide is better and more stably combined on a carrier and stably dispersed in water.

Further, the green environment-friendly type multi-component compound assistant is a phosphate-group-containing terpolymer NDF-04, a polycarboxy terpolymer, a dimethyl-monoperfluorodecylsebasic-polyethoxy-oleate multi-component compound, a dimethyl-monoperfluordecylsilyl-polyethoxy-stearate multi-component compound, a methyl-diperfluorodecylsebasic-polyethoxy-sodium benzenesulfonate multi-component compound, a methyl-diperfluorylsebasic-polyethoxy-stearate multi-component compound, a trifluoroperfluordecylsilyl-polyethoxy-sodium naphthalenesulfonate multi-component compound, a methyl-diperfluorylsebasic-polyethoxy-oleate multi-component compound, a triperfluorylsebasic-polyethoxy-stearate multi-component compound, a triperfluorylsebasic-polyethoxy-sodium benzenesulfonate multi-component compound, a poly-ethoxy-sodium benzenesulfonate multi-component compound, One or more of methyl-bis-perfluorodecyl silicon-polyethoxy-sodium naphthalene sulfonate polybasic compound, dimethyl-mono-perfluorodecyl silicon-polyethoxy-sodium benzene sulfonate polybasic compound, dimethyl-mono-perfluorodecyl silicon-polyethoxy-sodium naphthalene sulfonate polybasic compound and tri-perfluorodecyl silicon-polyethoxy-oleate polybasic compound.

Furthermore, the content of the graphene oxide in the emamectin benzoate nano slow-release pesticide preparation is 0.1-5%, the content of the emamectin benzoate is 0.1-5%, and the content of the green environment-friendly terpolymer auxiliary agent is 0-5%.

Furthermore, the content of the graphene oxide in the emamectin benzoate nano slow-release pesticide preparation is 0.5-2%, the content of the emamectin benzoate is 0.8-2.5%, and the content of the green environment-friendly terpolymer auxiliary agent is 0-2%.

Further, the rotating speed of the high-speed homogenizing machine is 1 k-20 k revolutions per minute, and the time for uniformly mixing is 5-120 minutes.

Furthermore, the rotating speed of the high-speed homogenizing machine is 5-15 k revolutions per minute, and the time for uniformly mixing is 15-60 minutes.

The invention provides a methylamino abamectin benzoate nano slow-release pesticide preparation prepared by the method.

The invention also provides application of the emamectin benzoate nano slow-release pesticide preparation prepared by the method in preventing and treating armyworms.

Has the advantages that: the invention constructs an emamectin benzoate delivery system based on graphene oxide as a carrier, and forms a stable, environment-friendly and multi-element compound additive and water without toxic organic solvent and additive, which has a slow release function, remarkably improves insect-resistant activity and is an environment-friendly emamectin benzoate nano slow-release pesticide preparation, wherein the drug loading rate of the slow-release pesticide preparation can reach 153.2 percent and is far beyond the drug loading rate of the existing graphene oxide pesticide delivery system at home and abroad. And the preparation process is simple, safe and environment-friendly, can be applied to prevention and control of agricultural pests, and has important significance for reducing application and improving efficiency of pesticides and sustainable development of agriculture.

Drawings

Fig. 1 is a scanning electron microscope image of the emamectin benzoate nano slow-release pesticide preparation in example 1 of the invention.

Fig. 2 is a graph showing the content change of the emamectin benzoate nano slow-release pesticide preparation in example 1 of the invention after being stored for 14 days at different temperatures.

Fig. 3 is a slow release curve of the emamectin benzoate nano slow release pesticide preparation in the embodiment 1 of the invention.

FIG. 4 is a diagram showing the insect-resistant effect of the emamectin benzoate nano slow-release pesticide preparation in example 1 of the invention.

Detailed Description

To further illustrate the present invention, the present invention is specifically illustrated below by a series of specific examples, wherein the graphene oxide used is a nano-scaled graphene oxide material, and all raw materials are commercially available.