阅读说明：本技术 一种防治桑轮纹病的生物杀菌剂及其制备方法 (Biological bactericide for preventing and treating mulberry ring spot and preparation method thereof ) 是由 李瑞雪 汪泰初 胡飞 王钰婷 陈明 夏家凤 田善富 高新文 于 2019-02-19 设计创作，主要内容包括：本发明公开了一种防治桑轮纹病的生物杀菌剂及其制备方法,生物杀菌剂按重量份计,包括如下组分：苦参碱18-26份,马齿苋25-35份,樟脑叶30-45份,甘草8-13份,纤维素酶4-7份,链霉菌40-60份和地衣芽孢杆菌20-40份。本发明公开的生物杀菌剂采用天然植物材料和菌液复配而成,对环境无污染,病原菌不容易产生抗药性；且能够渗透到桑树和轮纹病斑内部,干扰病原体代谢和繁殖,具有高效防治桑轮纹病的作用。(The invention discloses a biological bactericide for preventing and treating mulberry ring spot and a preparation method thereof, wherein the biological bactericide comprises the following components in parts by weight: 18-26 parts of matrine, 25-35 parts of purslane, 30-45 parts of camphor leaves, 8-13 parts of liquorice, 4-7 parts of cellulase, 40-60 parts of streptomyces and 20-40 parts of bacillus licheniformis. The biological bactericide disclosed by the invention is prepared by compounding natural plant materials and bacterial liquid, is free from environmental pollution, and pathogenic bacteria are not easy to generate drug resistance; and the composition can permeate into mulberry and physalospora piricola to interfere the metabolism and reproduction of pathogens, and has the effect of efficiently preventing and treating the physalospora piricola.)

1. The biological bactericide for preventing and treating the mulberry ring spot is characterized by comprising the following components in parts by weight: 18-26 parts of matrine, 25-35 parts of purslane, 30-45 parts of camphor leaves, 8-13 parts of liquorice, 4-7 parts of cellulase, 40-60 parts of streptomyces and 20-40 parts of bacillus licheniformis.

2. The biological bactericide for preventing and treating mulberry ring spot according to claim 1, which is characterized by comprising the following components in parts by weight: 23 parts of matrine, 30 parts of purslane, 38 parts of camphor leaves, 11 parts of liquorice, 6 parts of cellulase, 50 parts of streptomyces and 30 parts of bacillus licheniformis.

3. A preparation method of a biological bactericide for preventing and treating mulberry ring spot is characterized by comprising the following specific steps:

(1) weighing the components in parts by weight for later use;

(2) adding 100 parts of water into the purslane, camphor leaves and liquorice weighed in the step (1), wet-grinding for 20-30min under the condition of 2500-;

(3) and (3) fully and uniformly mixing the filtrate obtained in the step (2) with matrine, cellulase, streptomyces, bacillus licheniformis and 400 parts of water to obtain the biological bactericide.

4. The preparation method of the biological bactericide for preventing and treating mulberry ring rot according to claim 3, wherein the bacterial liquid concentration of streptomyces in the biological bactericide is 6x10⁶-8x10⁶cfu/mL, the bacterial liquid concentration of the bacillus licheniformis is 2x10⁶-4x10⁶cfu/mL。

Technical Field

The invention relates to the technical field of disease control, in particular to a biological bactericide for controlling mulberry ring spot and a preparation method thereof.

Background

At the early stage of ring spot, purplish red to purple brown frostbite-shaped spots appear on the leaves, and the enlarged spots are irregular; when part of disease spots develop to the leaf veins, the disease is suddenly aggravated, and the leaf veins are used as the top end and are withered in a V shape; the disease spots in the development process have reddish brown veins, dark green to yellow green peripheries and brown or dark brown insides; the disease spots of the lobules are less, and the lobules are in the shape of ring veins soon after the disease spots are formed into a plurality of small black spots.

When the ring rot occurs, oblate or elliptic reddish brown disease spots can appear on the trunk of a fruit tree by taking a bark hole as the center, in the second year of the disease, a plurality of conidiospores are generated in the centers of the disease spots, the disease spots and healthy parts are gradually deepened, diseased tissues are raised like saddles, and a plurality of disease spots are often connected together, so that the epidermis is rough; many fruit trees just entering the fruiting period are full of the lesion of the ring spot, and huge economic losses are caused to the fruit tree industry.

Wherein, the mulberry ring rot occurs in silkworm areas in each province of China. The pathogen (Spondylocladium moriSawada) belongs to the fungi of the subdivision Deuteromycotina. Pathogenic bacteria are over-wintered in diseases and soil, and are splashed to mulberry leaves along with wind and rain in the next year for primary infection and re-infection, so that the hidden and moist mulberry field has serious morbidity. The scabs are mostly appeared on the middle and lower leaves, after the leaves are infected by pathogenic bacteria, the scabs with different sizes are generated, the scabs have yellowish and reddish-brown bright concentric rings from the leaf surfaces, and the number of the rings is 1-5; the concentric ring lines of the disease spots on the leaf back are slightly less obvious, light brown, purple brown at the center, and densely and separately grown conidiophores and conidia. White hyphae are formed at the edge of the lesion.

At present, the main method for preventing and treating the ring spot is to spray chemical pesticide on trees, so that the prevention and treatment effect is poor, the trees are easy to generate pesticide resistance, the recurrence rate is high, and the environment is polluted by residual pesticide.

Therefore, the problem to be solved by the technical personnel in the field is to provide a biological bactericide for preventing and treating the mulberry ring spot and a preparation method thereof.

Disclosure of Invention

In view of the above, the invention provides a biological bactericide for preventing and treating the mulberry ring spot and a preparation method thereof.

In order to achieve the purpose, the invention adopts the following technical scheme:

a biological bactericide for preventing and treating mulberry ring spot comprises the following components in parts by weight: 18-26 parts of matrine, 25-35 parts of purslane, 30-45 parts of camphor leaves, 8-13 parts of liquorice, 4-7 parts of cellulase, 40-60 parts of streptomyces and 20-40 parts of bacillus licheniformis.

Further, the biological bactericide for preventing and treating the mulberry ring spot comprises the following components in parts by weight: 23 parts of matrine, 30 parts of purslane, 38 parts of camphor leaves, 11 parts of liquorice, 6 parts of cellulase, 50 parts of streptomyces and 30 parts of bacillus licheniformis.

Further, a preparation method of the biological bactericide for preventing and treating the mulberry ring spot disease comprises the following specific steps:

(1) weighing the components in parts by weight for later use;

(2) adding 100 parts of water into the purslane, camphor leaves and liquorice weighed in the step (1), wet-grinding for 20-30min under the condition of 2500-;

(3) and (3) fully and uniformly mixing the filtrate obtained in the step (2) with matrine, cellulase, streptomyces, bacillus licheniformis and 400 parts of water to obtain the biological bactericide.

Further, the concentration of the fungus liquid of streptomyces in the biological bactericide is 6x10⁶-8x10⁶cfu/mL, the bacterial liquid concentration of the bacillus licheniformis is 2x10⁶-4x10⁶cfu/mL。

According to the technical scheme, compared with the prior art, the invention discloses the biological bactericide for preventing and treating the mulberry ring spot and the preparation method thereof, the biological bactericide is prepared by compounding natural plant materials and bacterial liquid, the preparation method is simple, no pollution is caused to the environment, and pathogenic bacteria are not easy to generate drug resistance; and the composition can permeate into mulberry and physalospora piricola to interfere the metabolism and reproduction of pathogens, and has the effect of efficiently preventing and treating the physalospora piricola.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Streptomyces and Bacillus licheniformis were purchased.