阅读说明：本技术 一种植物酵素作为香精在化妆品中的应用 (Application of plant enzyme as essence in cosmetics ) 是由 黄正梅 于 2019-02-21 设计创作，主要内容包括：本发明涉及一种植物的酵素原液作为香精在化妆品中的应用。该发明首先将植物的花或茎叶或果皮加入水中,后补加糖类,密封自然发酵,最终得到的酵素液。利用所述酵素原液于化妆品中作为香精,可给化妆品带来香气,同时具有护肤,环保,安全,生产成本低的优点。(The invention relates to application of a plant enzyme stock solution as essence in cosmetics. Firstly, adding flowers, stems, leaves or fruit peels of plants into water, then adding sugars, sealing and naturally fermenting to finally obtain the enzyme solution. The ferment stock solution is used as essence in cosmetics, can bring fragrance to the cosmetics, and has the advantages of skin care, environmental protection, safety and low production cost.)

1. The application of a plant ferment as a flavor component in cosmetics is characterized in that the plant ferment is prepared by anaerobic fermentation of plant-derived components, saccharides and water, wherein the plant is selected from plants with pleasant aroma, such as roses, pomelos, mint and the like.

2. The plant ferment of claim 1, which is prepared by a preparation method comprising the following steps:

(1) mixing materials: weighing and uniformly mixing 0.5-2 parts of sugar, 0.5-3 parts of plant raw materials and 5-9 parts of water according to parts by weight to obtain a mixed solution;

(2) adjusting the pH of the mixed solution to 5-6 by using a pH regulator;

(3) carrying out anaerobic fermentation on the mixed liquor, wherein endophytes of plants are used as strains, the fermentation temperature is 20-35 ℃, the fermentation time is 1-6 months, and preferably 2-3 months;

(4) the plant ferment is added in the cosmetic in an amount of 0.1-5 wt%, preferably 0.3-3 wt%, and the cosmetic does not contain other essence components different from the plant ferment in the claims 1-3.

3. The plant ferment of claims 1-2, wherein said saccharide is selected from the group consisting of: one or more of glucose, sucrose, maltose, crystal sugar, brown sugar and white sugar.

4. The plant ferment prepared by the preparation method of claim 2.

Technical Field

The invention relates to the field of cosmetics, and in particular relates to application of a plant enzyme as essence in the field of cosmetics.

Background

Essence components are often added into the cosmetics, so that various large smells generated in the raw materials of the products and the stacking reaction are covered, and meanwhile, pleasant olfactory sensation can be brought to consumers.

The types of the essence are animal essence, plant essence and synthetic spice, and most of the essence components added in the cosmetics are synthetic spices. The production of synthetic fragrances often uses large amounts of chemical reagents, which has a number of disadvantages: firstly, the abuse of a large amount of chemical synthetic substances brings harm to the health of people; secondly, the purity of the product is reduced due to the lack of a specific substrate in the chemical synthesis; thirdly, the products of chemical synthesis often contain racemic mixtures, from which it is very difficult and expensive to extract the desired isomer or chiral perfume; fourth, people are increasingly disliked to chemically synthesized additives used in foods, cosmetics and the like and often cause skin irritation or allergic reaction when applied to cosmetics. With the demand of consumers for cosmetic additives, the cosmetic additives tend to be natural, healthy, safe and effective. The natural essence is a high-value fine chemical product, and the natural essence originally extracted from natural animals and plants is limited in raw materials and high in extraction cost, so that the market demand is far not met. Therefore, there is an increasing interest in the biosynthesis of flavors, and biotechnology will play an increasing role in the research and preparation of natural flavors and fragrances, which is also a challenge and opportunity for the development of biotechnology.

The fermentation engineering is a biological technology which is widely applied at present, and the natural perfume prepared by the fermentation engineering has the following characteristics:

(1) the method has mild conditions, realizes multi-step reaction which cannot be finished by a chemical method in the aqueous solution under mild conditions, has safe reaction, can be carried out at normal temperature and normal pressure, and has no explosion-proof requirement on production sites and equipment.

(2) High specificity and selectivity allows for specific selection of substrates, substrate binding sites, and reaction types, e.g., some natural chiral aromas can be produced.

(3) The fermentation is completed in good environment, and the three wastes formed after the product separation are less;

(4) aseptic production;

(5) the fermentation equipment has universality;

(6) overcomes the defects of low content of effective components, difficult separation, influence by climate, animal and plant diseases and the like existing as the only source of natural spices for a long time.

The spice obtained by fermentation has wide application in the field of food, and the natural edible spice which can be prepared by the microbial fermentation engineering at present mainly comprises the following components: natural acetoin, natural vanillin, natural phenethyl alcohol and the like. The method for producing the acetoin by the microbial fermentation method is an important method for producing the acetoin by a biological method, up to 20 microbial strains for high yield of the acetoin are screened and separated from the nature, the highest fermentation level of the acetoin is 80g/L at present, the acetoin is already industrialized by Shanghai Aipu spice Limited companies, and the fermentation process reaches the international leading level. Microbial fermentation is considered to be the most practical method for preparing natural vanillin at present. The research of producing natural vanillin by biological method mainly focuses on two aspects of microorganism species and substrate, and the conversion production of natural vanillin by using ferulic acid as substrate is also one of the hotspots of the current research. At present, natural ferulic acid extracted from rice bran oil is commonly used as a fermentation raw material, and the key point is the conversion rate in fermentation for preparing natural vanillin by fermenting with microorganism Sterptomyces. For example, Rodiya, France and Shanghai Aipu spice, Inc., the highest fermentation level reaches 15g/L, and the mass conversion rate is 60%. In the microbial fermentation method for producing natural phenethyl alcohol, phenylalanine and fluoroanilino alanine are used as raw materials, and the microbial beer yeast fermentation process is adopted to prepare the natural phenethyl alcohol, but the raw material cost is too high, and the conversion rate is not high for industrialized production. The Huabao aromatized science and technology development (Shanghai) company Limited successfully completes the production of natural phenethyl alcohol by a microbial fermentation method by taking tobacco (tobacco stems and tobacco powder) as raw materials through years of innovative research and development, and obtains invention patent authorization in 2004, 7 and 28 days (ZL patent No. 02137575.5, authorization notice CN 1159447C). The process comprises using agricultural byproduct tobacco as biotransformation precursor, adding appropriate culture medium, selecting appropriate strain (such as one of Candida utilis, Saccharomyces cerevisiae, and Kluyveromyces sinensis), performing fermentation culture under certain fermentation process conditions, degrading lignin, pectin and polyphenol compounds in tobacco, and converting into phenethyl alcohol. The purified phenethyl alcohol has pure natural fragrance, can be used as a natural spice for flavoring foods, and has wide industrial development prospect.

Patent application CN106912886A discloses a preparation method of a capsicum frutescens flavor essence, which comprises the following steps: taking pod pepper, naturally fermenting to prepare pickled pepper, cleaning pickled pepper, removing impurities, and crushing to obtain pickled pepper powder; mixing alcohol and propylene glycol according to a volume ratio of 1: 1, uniformly mixing to obtain a mixed solution, mixing the mixed solution with pickled pepper powder, leaching, and then distilling under reduced pressure to remove alcohol to obtain the pickled pepper powder.

Patent application CN101283773A discloses a method for preparing essence by whey powder, which is characterized by comprising the following steps: a. dissolving whey powder, and sterilizing; b. cooling the whey powder solution sterilized in the step a, inoculating, and performing fermentation culture; c. c, sterilizing the fermentation liquor treated in the step b, adding water for dilution and cooling, and then adding enzyme for enzymolysis; d. and c, performing rough filtration and fine filtration on the enzymatic hydrolysate treated in the step c, sterilizing and filling to obtain a finished product.

Patent application CN105462689A discloses a method for preparing essence by biotransformation of ionones by aspergillus niger, comprising the following steps: inoculating Aspergillus niger into a fermentation medium, adding ionone compounds, and finishing constant-temperature fermentation; adding a water-insoluble organic solvent into the fermentation liquor for extraction; and distilling the extract under reduced pressure until the solvent is completely removed to obtain the essence.

The natural perfume prepared by fermentation engineering has some difficulties, and the concentration of the fragrant compounds in the fermentation liquor is very low, so that the cost of the extraction process is high; secondly, there are some technical problems, and the process of producing the spice by microbial fermentation has two characteristics: firstly, the volatility and the lower water solubility of a plurality of fragrant substances make the fragrant substances difficult to recover, secondly, the product has the inhibiting and poisoning effects on the microorganism, the concentration of the final product in the fermentation liquor is always required to be maintained at a lower level, and secondly, the raw materials of some fermentation projects have high cost and are not economical. Thirdly, microbial strains with special transformation capacity need to be strictly screened, and the process needs to consume considerable research and development time and cost.

Therefore, a natural essence component suitable for cosmetics with low cost, strong fragrance and good stability still needs to be found.

Therefore, the invention relates to a plant enzyme used as essence applied to the field of cosmetics. The microbial community in the plant is used as a strain fermentation plant, and the method has the characteristics of low production cost, simplicity in operation, good water solubility, safety in source and environmental friendliness.

Disclosure of Invention

The invention relates to application of a plant enzyme as essence in cosmetics. The plant enzyme is prepared by adding flowers, stems, leaves or fruit peels of plants into water, then adding sugars, sealing and naturally fermenting by using endophytic microbiota of plant bodies as strains, and filtering to obtain filtrate.

The plant ferment is prepared by the following steps:

(1) mixing materials: weighing and uniformly mixing 0.5-2 parts of sugar, 0.5-3 parts of plant raw materials and 5-9 parts of water according to parts by weight to obtain a mixed solution;

(2) adjusting the pH of the mixed solution to 5-6 by using a pH regulator;

(3) carrying out anaerobic fermentation on the mixed solution, wherein the fermentation temperature is 20-35 ℃, and the fermentation time is 1-6 months; preferably 2-3 months.

(4) And filtering the fermented mixed solution to obtain the plant enzyme stock solution.

The plant material comprises one or more components of flower, stem leaf, pericarp and seed of plant.

Further, the plant material is selected from plants with pleasant fragrance, such as rose petals, shaddock peel or mint leaves, sweet osmanthus, pineapple, orange and the like.

The sugar can be one or more of glucose, sucrose, maltose, crystal sugar, brown sugar and white sugar.

The pH regulator can be one or a mixture of citric acid and phosphoric acid.

Description of the drawings:

FIG. 1 is a graph showing the change in skin water content of rose essence/ferment

FIG. 2 is a diagram showing the change of skin water content of grapefruit essence/ferment

FIG. 3 is a graph showing the change in skin moisture content of mint flavor/enzyme

FIG. 4 is a graph showing changes in skin melanin values with rose essence/ferment

FIG. 5 is a graph showing the change in skin melanin values of grapefruit essence/ferment

FIG. 6 is a graph of changes in the melanin values of peppermint/enzyme skin

TABLE 1 volunteer fragrance perception score table

Detailed description of the invention

In order to better understand the content of the invention, the description is further combined with some specific implementation examples.