阅读说明：本技术 一种多功能异质结构微纤维的制备方法 (Preparation method of multifunctional heterostructure microfiber ) 是由 陈苏 黄秋 于 2020-06-05 设计创作，主要内容包括：本发明涉及一种多功能异质结构微纤维的制备方法；将有机溶剂溶解的疏水性聚合物溶液与水溶解的亲水性聚合物溶液分别作为内相和外相流体,利用在纺丝过程中亲水性聚合物与钙离子发生离子交联作用以及亲水性聚合物对疏水性流体的包裹作用原理,可通过简单的微流体装置实现一种多功能异质结构微纤维的快速连续化制备。本发明制备的异质结构微纤维具有双重载药功能,研究表明该双重载药微纤维具有很好的止血凝血性能,将该双载药微纤维编织成人造皮肤支架对伤口愈合具有良好的促进作用,有望为新型伤口敷料、人造皮肤等领域的开发提供重要的技术支撑。(The invention relates to a preparation method of multifunctional heterostructure microfiber; the hydrophobic polymer solution dissolved by an organic solvent and the hydrophilic polymer solution dissolved by water are respectively used as an internal phase fluid and an external phase fluid, and the rapid continuous preparation of the multifunctional heterostructure microfiber can be realized by a simple microfluid device by utilizing the principle that the hydrophilic polymer and calcium ions generate ionic crosslinking effect and the hydrophilic polymer wraps the hydrophobic fluid in the spinning process. The heterostructure microfiber prepared by the invention has a double drug loading function, researches show that the double drug loading microfiber has good hemostatic and blood coagulation performance, and the artificial skin scaffold woven by the double drug loading microfiber has a good promotion effect on wound healing, so that the heterostructure microfiber is expected to provide an important technical support for the development of the fields of novel wound dressings, artificial skin and the like.)

1. A preparation method of multifunctional heterostructure microfiber comprises the following specific steps:

A. uniformly dissolving a hydrophobic polymer in an organic solvent and doping an oil-soluble drug to obtain an internal phase spinning solution; uniformly dissolving a hydrophilic polymer in water and adding a water-soluble drug to obtain an external phase spinning solution;

B. injecting the two prepared spinning solutions into a microfluid device as an internal phase fluid and an external phase fluid respectively, and finally introducing the two prepared spinning solutions into a receiving device filled with a curing agent for curing and forming; the multifunctional heterostructure microfiber is obtained by continuously adjusting spinning process parameters.

2. The method according to claim 1, wherein the hydrophobic polymer in step a is polylactic acid or a polylactic acid-glycolic acid copolymer; the hydrophilic polymer is sodium alginate or sodium polyacrylate; the organic solvent is dichloromethane; the oil-soluble medicine is emu oil or tetrahydrocurcumin; the water-soluble medicine is nano silver or fibrinogen.

3. The preparation method according to claim 1, wherein the mass fraction of the hydrophobic polymer in the internal phase spinning solution is 2-5%, and the mass fraction of the oil-soluble drug is 0.03-0.05%; the mass fraction of the hydrophilic polymer in the external phase spinning solution is 1-2.5%, and the mass fraction of the water-soluble drug is 0.02-0.03%.

4. The method according to claim 1, wherein the curing agent in step B is CaCl₂The mass concentration of the solution is 5-10%.

5. The preparation method according to claim 1, wherein the spinning process parameters in step B are: the inner phase outlet diameter of the microchip in the microfluidic device is 140-; the flow rate of the fluid of the inner phase is 3-7mL/h, and the flow rate of the fluid of the outer phase is 5-15 mL/h.

Technical Field

The invention relates to a preparation method of multifunctional heterostructure microfiber, in particular to a rapid continuous preparation method of multifunctional microfiber by adopting a microfluid wet spinning technology.

Background

The heterostructure microfiber is widely concerned due to the advantages of the structure and the performance, the functional heterostructure microfiber can be constructed by means of electrostatic spinning, microfluidic wet spinning and the like, and the prepared heterostructure microfiber has a plurality of applications in the fields of sensing technology, photoelectric device, photocatalysis, biotechnology and the like. At present, how to apply the heterostructure microfiber to the biomedical field becomes a hot research focus of the heterostructure microfiber. The microfluid wet spinning technology is an ideal reactor platform for producing the microfibers with the multilevel structure, and the morphology, the size and the composition of the microfibers can be accurately regulated and controlled by the microfluid wet spinning technology. Therefore, ordered heterostructure microfibers with stable structures can be constructed by microfluidic wet spinning techniques. The invention combines the ionic crosslinking principle to realize the rapid solidification of the external phase polymer fluid, and continuously prepares the functional microfiber with the heterostructure through the wrapping effect of the external phase polymer fluid on the internal phase polymer fluid.

Disclosure of Invention

The invention aims to solve the technical problems that the research of the heterostructure fiber material in the biomedical field is not deep enough, the multifunctional heterostructure microfiber is rapidly and continuously prepared by a simple microfluid wet spinning technology and is successfully applied to the biomedical field. The functional material has simple preparation method and low cost, and can be successfully applied to the fields of blood coagulation and hemostasis and wound healing promotion. In order to achieve the above purpose, a method for preparing multifunctional heterostructure microfiber is provided, a simple microfluidic device is designed, and functional heterostructure microfiber is obtained by continuously adjusting spinning process parameters.

The technical scheme of the invention is as follows: a preparation method of multifunctional heterostructure microfiber comprises the following specific steps:

A. uniformly dissolving a hydrophobic polymer in an organic solvent and doping an oil-soluble drug to obtain an internal phase spinning solution;

uniformly dissolving a hydrophilic polymer in water and adding a water-soluble drug to obtain an external phase spinning solution;

B. injecting the two prepared spinning solutions into a microfluid device as an internal phase fluid and an external phase fluid respectively, and finally introducing the two prepared spinning solutions into a receiving device filled with a curing agent for curing and forming; by continuously adjusting the parameters of the spinning process, such as:

the diameters of the outlets of the inner phase and the outer phase of the microchip and the flow rates of the fluid of the inner phase and the outer phase are used for obtaining the functional heterostructure microfiber.

Preferably, the hydrophobic polymer in the step A is polylactic acid or polylactic acid-glycolic acid copolymer; the hydrophilic polymer is sodium alginate or sodium polyacrylate; the organic solvent is dichloromethane; the oil-soluble medicine is emu oil or tetrahydrocurcumin; the water-soluble medicine is nano silver or fibrinogen.

Preferably, the mass fraction of the hydrophobic polymer in the internal phase spinning solution is 2-5%, and the mass fraction of the oil-soluble drug is 0.03-0.05%; the mass fraction of the hydrophilic polymer in the external phase spinning solution is 1-2.5%, and the mass fraction of the water-soluble drug is 0.02-0.03%.

Preferably, the curing agent in the step B is CaCl₂The mass concentration of the solution is 5-10%.

Preferably, the spinning process parameters in the step B are as follows: the inner phase outlet diameter of the microchip in the microfluidic device is 140-; the flow rate of the fluid of the inner phase is 3-7mL/h, and the flow rate of the fluid of the outer phase is 5-15 mL/h.

Has the advantages that:

1. the heterostructure microfiber prepared by the invention has the double-medicine double-speed release function and has the effects of quickly stopping bleeding and promoting wound healing.

2. The preparation method is simple in preparation process and low in production cost, and can realize rapid macro preparation of the multifunctional heterostructure microfiber.

3. The heterostructure dual-drug loaded microfiber prepared by the method lays a foundation for researches on artificial skin, novel medical materials and the like.

Drawings

FIG. 1 is a schematic representation of the preparation of a multifunctional heterostructure microfiber according to the present invention; in the figure, 1-a two-channel microflow pump, 2-a first injection pump, 3-a second injection pump, 4-a 1 capillary, 5-a 2 capillary, 6-a core-shell microchip, 7-a 3 capillary and 8-a receiving device are arranged.

Detailed Description

The present invention is further illustrated by the following specific examples, but the present invention is not limited to only the following examples. A schematic of the preparation of a multifunctional heterostructure microfiber is shown in figure 1.