阅读说明：本技术 一种含生物活性玻璃的复配液及其制备方法 (Compound liquid containing bioactive glass and preparation method thereof ) 是由 刘伟 刘阔 史光明 于 2021-07-13 设计创作，主要内容包括：本发明涉及一种含生物活性玻璃的复配液及其制备方法,特征在于本复配液在制备过程中将生物活性玻璃和小分子活性物质进行了复配。本方法将生物活性玻璃粉剂与一定浓度和分子量的小分子活性物质混合,使生物活性玻璃分子与小分子活性物质形成均一稳定的溶液体系,利用生物活性玻璃分子释放出的离子和活性小分子发生协同增效作用,进一步增强了溶液的功效。本发明得到的复配液具有美白、保湿、抗氧化的功效,可祛除皱纹,增加皮肤弹性,修复皮肤损伤,对发红、过敏皮肤具有较好的缓解作用。(The invention relates to a compound liquid containing bioactive glass and a preparation method thereof, which is characterized in that the bioactive glass and small molecular active substances are compounded in the preparation process of the compound liquid. The method mixes the bioactive glass powder with micromolecular active substances with certain concentration and molecular weight to form a uniform and stable solution system of the bioactive glass molecules and the micromolecular active substances, and utilizes ions released by the bioactive glass molecules and active micromolecules to generate a synergistic effect to further enhance the effect of the solution. The compound liquid obtained by the invention has the effects of whitening, moisturizing and resisting oxidation, can remove wrinkles, increase the skin elasticity and repair skin injury, and has a good relieving effect on reddened and allergic skin.)

1. The invention relates to a compound liquid containing bioactive glass and a preparation method thereof, which is characterized in that bioactive glass and micromolecular active substances are compounded in the preparation process of the compound liquid, and bioactive glass powder is mixed with the micromolecular active substances with certain concentration and molecular weight, so that bioactive glass molecules and the micromolecular active substances form a uniform and stable solution system.

2. The bioactive glass of claim 1 is one of 58S, 77S and 45S5, has a concentration of 0.1-5%, and has a particle size of no more than 100 nm.

3. The small molecule active substance in claim 1 is one or more of hydrolyzed collagen, active peptide, amino acid, vitamin, ceramide, sodium hyaluronate, fullerene, plant extract, etc., with concentration of 0.1-5%, and molecular weight of no more than 2000 Da.

4. A composition for human administration, which comprises the compound liquid obtained in claim 1 and one or more of glycerol, 1, 2-hexanediol, p-hydroxyacetophenone, caprylyl hydroximic acid, 1, 2-pentanediol, and ethylhexyl glycerin.

5. A composition for human administration, which comprises the compound liquid obtained from claim 1 and one or more of glycerol, methylparaben, phenoxyethanol, EDTA-2Na, etc.

6. The compound liquid and the composition as described in claim 1 are used in the field of cosmetics or medical and cosmetic products.

Technical Field

The invention belongs to the field of medicines and cosmetics, and particularly relates to a compound liquid containing bioactive glass and a preparation method thereof.

Background

Bioactive glass (BAG) is made of SiO₂，Na₂O, CaO and P₂O₅And silicate glass material composed of basic components can be combined with tissue bonds to repair, replace and regenerate body tissues. The alkali metal and alkaline earth metal ions bound to non-bridging oxygens in the glass network are readily soluble in the presence of an aqueous medium to release ions of multiple valencies, e.g. Na⁺、Si⁴⁺、P⁵⁺、Ca²⁺And further a series of biological reactions, which is an important reason why the BAG has good biocompatibility with bioactive tissues.

The chemical composition of BAG is similar to human skeleton, and the dissolved ions can promote the generation of bone growth factor and the proliferation of osteoblast, and can be widely applied to the field of bone repair. BAG can also be made into gel dressing for repairing acute and chronic skin wound and mucosa ulcer, and has effects of relieving inflammation and pain. Bioactive glass is the only bioactive material that has been able to bond with bone tissue and to attach to soft tissue.

The BAG has excellent performance, so that the BAG is widely applied, most of the BAG is used for wound repair, the BAG is rarely used in the fields of medicine, beauty and cosmetic skin care, and particularly, the BAG is rarely reported to be used in combination with small-molecule active substances.

Disclosure of Invention

In the invention, the BAG and the micromolecule active substance are compounded to generate a synergistic interaction effect, so that the effect of the micromolecule active substance is enhanced.

A compound liquid of bioactive glass and a preparation method thereof are characterized in that the compound liquid compounds the bioactive glass and micromolecule active substances in the preparation process. Mixing bioactive glass powder with small molecular active substances with certain concentration and molecular weight to form a uniform and stable solution system.

The purpose of the invention can be realized by the following technical scheme:

raw materials meeting the requirements are prepared, taking 100g of compound liquid as an example, and functional components comprise 0.1-5g of micromolecule active substances, 0.1-5g of bioactive glass and 90-99.8g of dispersion medium solution.

The bioactive glass is one of 58S, 77S and 45S5, the concentration is 0.1-5%, and the particle size is not more than 100 nm. The small molecule active substance is one or more of hydrolyzed collagen, amino acid, active peptide, vitamin, ceramide, sodium hyaluronate, fullerene, plant extract, etc., and has a molecular weight of no more than 2000Da and a concentration of 0.1-5%.

The compound liquid can be directly used after being added with a preservative system, and can also be used together with other functional components.

Drawings

FIG. 1 is a graph of skin elasticity tests using a compound fluid containing BAG, a solution without BAG and a solution containing only a single component BAG.

Detailed Description

The present invention is further illustrated by the following specific examples, which are intended to be illustrative only and not to limit the scope of the invention. All the technologies realized based on the above contents of the present invention belong to the scope of the present invention. The experimental methods used in the following examples are all conventional methods unless otherwise specified; the materials, reagents and the like used are commercially available unless otherwise specified.

Example 1

Preparation of a bioactive glass-containing solution:

preparing raw materials meeting the requirements, taking 100g of solution as an example, adding 10g of glycerol, 4g of squalane, 3g of lanolin, 3g of white oil, 3g of isooctyl palmitate and 2g of glycerol oleate into 100g of deionized water, and quickly stirring uniformly for 1 hour to prepare a dispersion medium solution.

0.5g of p-hydroxyacetophenone, 0.5g of 1, 2-hexanediol, 0.5g of bioactive glass powder and 98.5g of the dispersion medium solution are taken, fully mixed and stirred for 2 hours to obtain a bioactive glass-containing solution.

Example 2

Preparation of a compound liquid containing bioactive glass:

1. preparing raw materials meeting requirements, and preparing a compound solution with functional components of 2g of hydrolyzed collagen, 2g of nicotinamide, 2g of ceramide, 0.1g of hyaluronic acid, 0.2g of aloe extract and 0.5g of centella extract.

2. After 0.5g of p-hydroxyacetophenone, 0.5g of 1, 2-hexanediol, 1g of bioactive glass powder and 91.2g of the dispersion medium prepared by the method in example 1 were mixed, the mixture was uniformly stirred for 2 hours to obtain a composite solution containing bioactive glass.

Example 3

Preparation of a compound liquid containing bioactive glass:

1. preparing raw materials meeting the requirements, and preparing a compound solution with functional components of 2g of hydrolyzed collagen, 2g of nicotinamide, 2g of ceramide, 0.1g of hyaluronic acid, 0.2g of aloe extract, 0.5g of centella asiatica extract and 0.5g of salvia miltiorrhiza extract.

2. 0.5g of p-hydroxyacetophenone, 0.5g of 1, 2-hexanediol, 0.5g of bioactive glass powder and 91.2g of the dispersion medium prepared by the method in example 1 were taken, and after mixing the above components, the mixture was uniformly stirred for 2 hours to obtain a composite liquid containing bioactive glass.

Example 4

Preparation of a bioactive glass-free solution:

1. preparing raw materials meeting the requirements, wherein the functional components of the prepared solution comprise 2g of hydrolyzed collagen, 2g of nicotinamide, 2g of ceramide, 0.1g of hyaluronic acid, 0.2g of aloe extract, 0.5g of centella asiatica extract and 0.5g of salvia miltiorrhiza extract.

2. 0.5g of p-hydroxyacetophenone, 0.5g of 1, 2-hexanediol and 91.7g of the dispersion medium prepared in example 1 were mixed and stirred uniformly for 2 hours to obtain a solution containing no bioactive glass.

Example 5

Measurement of skin elasticity:

volunteers recruited close in skin were divided into three groups. The same parts of three groups of volunteers are subjected to blank test by using a skin elasticity tester, and blank values are determined. Smearing the compound liquid in example 3 on the volunteer subjects in the group 1 once a day; group 2 volunteers were smeared once daily with the solution without BAG ingredient of example 4 as a control; group 3 volunteers were painted with the one-component BAG solution of example 1 once daily. Skin elasticity was measured 2, 4, 8, 14 days after the start of application. The results show that the BAG formulation group increased skin elasticity with increasing days of application by 10% after two weeks, whereas the no BAG group and the one-component BAG group did not increase skin elasticity with increasing days of application by a significant amount, only 3.6% and 2.3%. Illustrating the efficacy of BAG in synergistically increasing the elasticity of the skin, the specific elasticity increase curves for the three groups of solutions are shown in figure 1.

Example 6

Measurement of moisture retention:

volunteers recruited close in skin were divided into three groups. Smearing the compound liquid in example 3 on the 1 st group of volunteer subjects, and beating until the absorption is uniform; the volunteers in group 2 were applied to the same sites with the control solution of the same concentration without adding BAG of example 4; group 3 volunteers were smeared with the one-component BAG solution of example 1. The skin moisture content was measured 0 hour, 1 hour, 2 hours, 4 hours, and 8 hours after the start of application using a skin moisture tester (SG-9D). Test results show that the compound liquid containing the BAG has a good moisturizing effect, which indicates that the BAG has a synergistic moisturizing effect. Specific tests are shown in table 1:

table 1 skin moisture test meter for different groups of solutions

Example 7

Measurement of whitening effect:

volunteers with similar skin shade were recruited into three groups. Group 1 volunteers were applied with the compound solution of example 3, group 2 volunteers were applied to the same sites with the control solution of example 4 without the same concentration of BAG, and group 3 volunteers were applied with the one-pack BAG solution of example 1 once a day at night. Changes in skin color difference Δ E were measured 15 days, 30 days, 45 days, and 60 days after application using a Lab spectrocolorimeter.

The results showed a significant change in skin color in the BAG group subjects after 60 days of use,^§P<0.05; no change was evident after 60 days of use in the other BAG-free group trial and the single BAG group. Thus, the BAG has the efficacy of synergistic whitening. Specific color difference changes are shown in table 2:

TABLE 2 comparison of color difference Δ E at different time periods after use of different sets of solutions

Example 8

And (3) measuring the antioxidant capacity:

DPPH is described as a stable free radical in solution with a purple color and strong absorption at 510 nm. When the free radical scavenger exists, single electrons of DPPH are paired, so that the color of the solution is lightened, the absorbance value is reduced, and the inhibition rate of free radicals of the substances can be evaluated, and further the antioxidant capacity is evaluated.

The inhibition ratio formula is as follows: y (%) =1- (A-B)/C

Wherein: a is the absorbance of the sample solution and DPPH solution

B is the absorbance of the sample solution

C is the absorbance of DPPH solution

Y is the inhibition ratio.

Three groups of solutions were prepared, 1 group of BAG0.5g, hydrolyzed collagen 2g, deionized water 97.5g, 2 group of hydrolyzed collagen 2g, deionized water 98g, 3 group of BAG0.5g and deionized water 99.5 g.

Taking 4 test tubes, numbering 1,2, 3 and 4, adding 3ml of the solution in the 1 st group and 3ml of water into the 1 st test tube, adding 3ml of the solution in the 2 nd group and 3ml of water into the 2 nd test tube, adding 3ml of the solution in the 3 rd group and 3ml of water into the 3 rd test tube, adding 3ml of the DPPH reagent and 3ml of water into the 4 th test tube, testing absorbance at the wavelength of 510nm, and marking as B₁、B₂、B₃C, the test result is B₁=0.167，B₂=0.134，B₃=0.235，C=0.945。

Another 3 test tubes are taken out of the test tube,test tubes No. 5, 6, 7, 5 were filled with 3ml of group 1 solution and 3ml of DPPH reagent, test tube No. 6 was filled with 3ml of group 2 solution and 3ml of DPPH reagent, test tube No. 7 was filled with 3ml of group 3 solution and 3ml of DPPH reagent, and after mixing for half an hour, the absorbance at 510nm wavelength, denoted A₁、A₂The result of the A3 test was A₁=0.387，A₂=0.635，A₃=0.713。

Inhibition ratio Y (%) =1- (A) of group 1₁-B₁）/C=76.7%

Group 2 inhibition ratio Y (%) =1- (a)₂-B₂）/C=47.0%。

Inhibition ratio Y (%) =1- (A) of group 3₃-B₃）/C=49.4%

Group 1 had a higher free radical inhibition rate, indicating that BAG has a synergistic antioxidant effect.

Example 9

Measurement of wrinkle-removing ability:

volunteers with similar skin fold were recruited into three groups. Group 1 used the solution prepared in example 1, group 2 used the solution prepared in example 3, and group 3 used the solution prepared in example 4, and was observed for 1 month after use, during which external corner wrinkles were applied once a day. A3D image of the skin surface structure of each volunteer was obtained using a skin wrinkle tester, the image data was processed with a computer, the texture and the shape and texture of the wrinkles were quantitatively analyzed to obtain the volume of wrinkles, and the difference DeltaV between the wrinkle volume for 1 month before and after each group was measured. The result shows that the BAG compound liquid has better wrinkle-removing effect, and the effect of the BAG and the functional liquid which are used independently is not as good as that of the compound liquid, which shows that the BAG has the effect of removing wrinkles synergistically. The specific test results are shown in table 3:

TABLE 3 comparison of wrinkle volume difference DeltaV after one month using different sets of solutions

Example 10

Measurement of skin soothing repair capacity:

three volunteers with similar skin sensitivity were recruited to make gross observations of the facial condition and record the redness index and the pain index with lactic acid smeared at the sensitive site. The solution prepared in example 1 was used by person 1, the solution prepared in example 3 was used by person 2, and the solution prepared in example 4 was used by person 3, and applied once a day. The general condition of the face was observed after 1 week, 2 weeks, 3 three weeks, 4 weeks, and the sensitive area was smeared with lactic acid, and the redness index and pain index were recorded.

The results show that when BAG ingredient is used alone, the recovery of facial appearance is more obvious, and the pain stimulation to skin is not improved obviously; when only the small molecule active solution is used, the facial recovery is not obvious, but the pain stimulation to the skin is relieved; when the BAG compound liquid is used, the appearance and pain of the skin are recovered and relieved to a certain extent. To a certain extent BAG has the function of repairing damaged sensitive muscle barriers, while the small molecular active substance has the function of relieving high sensitive skin nerve receptors, and when the two are used simultaneously, the BAG has the functions of repairing and relieving the skin.

TABLE 4 appearance and pain index of the back of each volunteer after different weeks of application

Note: redness index: 5. very red 4, red 3, light red 2, reddish 1, natural skin color; pain index: 5. 4, 3, 2, 1, and no pain.