阅读说明：本技术 一种防治草地贪夜蛾的生物制剂及其使用方法 (Biological agent for preventing and treating spodoptera frugiperda and using method thereof ) 是由 任学祥 叶正和 苏贤岩 迟雨 徐丽娜 陈浩梁 于 2020-03-05 设计创作，主要内容包括：本发明公开了一种防治草地贪夜蛾的生物制剂,具体涉及根瘤菌用于防治玉米、小麦等作物上草地贪夜蛾的危害。针对新入侵害虫草地贪夜蛾开展实施此项技术,可大大减少草地贪夜蛾对玉米、小麦等农作物的危害,对粮食的稳产意义重大,具有及其显著的经济和社会效益。(The invention discloses a biological agent for preventing and treating spodoptera frugiperda, and particularly relates to a method for preventing and treating spodoptera frugiperda harm on crops such as corn and wheat by using rhizobia. The technology is carried out aiming at the newly invaded pest spodoptera frugiperda, so that the harm of the spodoptera frugiperda to crops such as corn, wheat and the like can be greatly reduced, the significance on the stable yield of grains is great, and the economic and social benefits are obvious.)

1. A biological preparation for preventing and treating Spodoptera frugiperda is characterized in that the main component of the biological preparation is rhizobia.

2. The biological preparation for controlling spodoptera frugiperda as claimed in claim 1, characterized in that the method of use is seed coating, dressing or soaking.

Technical Field

The invention belongs to the field of chemical control of pests, and particularly relates to a biological agent for controlling Spodoptera frugiperda and a using method thereof.

Background

Spodoptera frugiperdaSpodoptera frugiperda(Smith) is a parasitic agricultural pest newly invading China, and mainly harms corn and rice, and also harms crops such as wheat, potato, sugarcane and the like. Feeding leaves of the larvae may cause defoliation and subsequent transferIt is harmful. Sometimes a large number of larvae are damaged by root-cutting, cutting off the stems of seedlings and young plants, causing great losses. On larger crops, such as corn ears, the larvae can dig into the crop. When eating corn leaves, a large number of holes are left. After the young larva eats, the leaf vein is in the shape of window screen. Old larvae, like the rootworms, can cut 30-day old seedlings along the base. When the larva can penetrate into the ear of the plant with large booting ear and is harmful to tomato, the bud and growing point can be taken and penetrated into the fruit. When the population is large, the larvae are like a march and spread in groups. When the environment is favorable, the weeds are often left behind. The adult can fly and spread for a certain distance. Spodoptera frugiperda has become an important pest that harms food production in China.

Rhizobiaum (Rhizobium), a type of rod-shaped bacteria that symbiotic with legumes, form nodules and fix the nitrogen in the air for plant nutrition. A class of gram-negative aerobic bacilli capable of promoting abnormal proliferation of plants. Within the nodule, the rhizobia imbibes carbohydrates, mineral salts, and water from the cortical cells of the legume roots. To grow and propagate. At the same time, they fix the free nitrogen in the air by the action of fixing nitrogen, and convert it into nitrogen-containing compound which can be used by plant for plant life. Thus, the rhizobia and the root form an interdependent symbiotic relationship. The rhizobium inoculant is a microbial preparation product prepared by taking rhizobium as a production strain, and can fix nitrogen elements in the air and provide a large amount of nitrogen fertilizer for host plants.

The national recommended emergency medicament for preventing and treating spodoptera frugiperda comprises emamectin benzoate, methiocarb, tetrachlorantraniliprole, chlorantraniliprole, lambda-cyhalothrin, deltamethrin and other medicaments, and most of the compound medicaments are compounded of emamectin benzoate. The invention finds that the rhizobium inoculant can play a good evasion role on spodoptera frugiperda after coating crop seeds in research, which is a new discovery that has not been reported by the prior people.

Disclosure of Invention

1. Objects of the invention

The invention aims to provide a biological agent for preventing and treating spodoptera frugiperda and a use method thereof.

2. Technical scheme

The invention develops a biological agent for preventing and controlling spodoptera frugiperda and a using method thereof through an indoor pot experiment, and the specific component is rhizobia; the components are processed into related preparations and then used by coating crop seeds (corn, wheat and the like) according to a certain proportion to reduce the harm of spodoptera frugiperda.

The invention provides a biological preparation for preventing and treating Spodoptera frugiperda, which is characterized in that the main component of the biological preparation is rhizobia, and the application method comprises the steps of coating, seed dressing and seed soaking of crop seeds (corn, wheat and the like) according to a certain proportion. Can effectively reduce the harm of spodoptera frugiperda, and has good effects of protecting seedlings and increasing yield.

Compared with the existing prevention and control measures, the invention has obvious technical advantages and can produce the following beneficial effects:

(1) the developed biological agent belongs to bacteria and does not belong to the category of chemical pesticides, and the rhizobia is applied to the control of spodoptera frugiperda and has not been reported by the predecessors;

(2) the method can realize effective avoidance of Spodoptera frugiperda, and the nitrogen fixation effect of the rhizobia has a good promoting effect on the early emergence of crops, so that the seedling protection, seedling strengthening and seedling alignment effects are achieved.

(3) The application of the invention can greatly reduce the use of chemical pesticides and reduce the pollution of the chemical pesticides to the environment, and the effect is obvious.

Description of the drawings: FIG. 1 shows the comparison of leaf weight reduction of 22h maize and FIG. 2 shows the comparison of leaf weight reduction of 23h wheat

Detailed Description

Taking the rhizobia aqua as an example (but not limited to the application of any rhizobia preparation), the avoidance effect of the rhizobia aqua on spodoptera frugiperda after coating corn and wheat seeds with the preparation is tested.

Experimental data

Firstly, a test reagent: rhizobium water aqua

II, test work: corn and wheat

Thirdly, a determination method:

the method adopts the method of measuring the indoor potted plant and the culture dish.

The rhizobia is coated (or soaked and mixed) on the seeds of corn and wheat according to a proportion and then sown in a plastic basin by adopting an inoculation method, and the uncoated control is adopted. Every plastic basin seeding seed after 50, 100 coatings, after the crop seedling, cut the blade and put in the culture dish (the district is distinguished to the mark), put into 1 on the spodoptera frugiperda larva, record both sides blade weight, make clear and determine the rhizobia and to the effect of avoiding of spodoptera frugiperda.

The attached figures 1 and 2 show the avoidance effect of rhizobium coatings on spodoptera frugiperda of corn and wheat seeds. See figures 1 and 2.

As can be seen from the attached drawings 1 and 2, the rhizobium-coated corn and wheat leaves have a remarkable evasion effect on Spodoptera frugiperda, in other words, Spodoptera frugiperda does not like the corn and wheat leaves treated by edible rhizobium, so that the differences are remarkable, and the repeatability is strong.

As can be seen from the above, the rhizobia has a good application prospect in the aspect of preventing and controlling Spodoptera frugiperda.

In addition, crops producing rhizobia can be vigorously popularized to crop rotation with corn, wheat and the like, for example, a wheat-bean rotation mode is used for replacing a wheat-corn rotation mode.