阅读说明：本技术 一种鳄鱼提取物在抗癌中的应用 (Application of crocodile extract in anticancer ) 是由 张树华 于 2020-05-11 设计创作，主要内容包括：本发明提供一种鳄鱼提取物在制备抗癌药物或者抗癌辅助药物的制备中的应用方式,属于鳄鱼提取物应用技术领域,所述的鳄鱼提取物是鳄鱼多肽,其通过对鳄鱼组织进行加热软化,再进行高温恒温水解,再对水解产物进行浓缩干燥得到鳄鱼多肽提取物粉末,具有制作流程简单、低成本和具有一定的抑癌活性的优点。(The invention provides an application mode of a crocodile extract in preparation of an anti-cancer drug or an anti-cancer auxiliary drug, which belongs to the technical field of crocodile extract application.)

1. An application of crocodile extract in preparing anticancer medicine or anticancer adjuvant medicine is provided.

2. The use of crocodile extract according to claim 1, wherein the crocodile extract is one or a combination of crocodile bone polypeptide or crocodile meat polypeptide.

3. The use of crocodile extract according to claim 2, wherein the crocodile bone polypeptides and crocodile meat polypeptides are made by the steps of:

step S10, taking crocodile tissues, fully cleaning, and heating and hydrolyzing the cleaned crocodile tissues;

step S20, standing and precipitating the hydrolysate obtained in the step S10, and filtering to obtain a filtrate which is a crude extract;

step S30, adding diatomite and active carbon into the crude extract obtained in the step S20, stirring, standing for precipitation again, and filtering to obtain filtrate as a fine extract;

step S40, drying the refined extract obtained in step S30 to obtain crocodile peptide powder;

the crocodile tissue is crocodile bone or crocodile meat.

4. The use of crocodile extract according to claim 3, wherein the heating hydrolysis in step S10 is to put crocodile tissue into distilled water and heat and stir in an oil bath at 110-130 ℃ for 7-9 h; the mass ratio of the crocodile tissues to the distilled water is that the crocodile tissues: distilled water 1: 5.

5. the use of crocodile extract according to claim 3, wherein the step S10 further comprises a step S11 of soaking crocodile tissue in clear water at 70-80 ℃ for 25-35 min.

6. The method for extracting crocodile polypeptide according to claim 3, wherein the step S40 comprises a step S41, wherein the refined extract obtained in the step S30 is concentrated by a vacuum water pump to obtain a concentrated extract.

7. The method for extracting crocodile polypeptide according to claim 6, wherein the step S40 comprises a step S42, and the concentrated extract obtained in the step S41 is dried into powder by using a freeze dryer.

Technical Field

The invention belongs to the technical field of crocodile extract application, and particularly relates to application of crocodile extract in resisting cancers.

Background

Crocodile belongs to vertebrate reptilia, widely grows in south Asia countries such as Vietnam, Thailand, Malaysia and the like, and has the characteristics of large body size, strong reproductive capacity, fast growth, strong disease resistance and the like as the only existing offspring of grandma. The whole body of the crocodile is precious, and the meat, bones, nails and internal organs of the crocodile contain rich and excellent protein, amino acid, unsaturated fatty acid, vitamins and various trace elements which are necessary for human bodies.

The protein is used as a nutrient essential to human body and is very important to the life health of human body. Epidemiological investigation results show that if the daily protein intake is too low, esophageal cancer, gastric cancer, liver cancer and the like are easy to suffer. In addition, a large number of scientific researches show that a large number of bioactive polypeptides can be obtained by hydrolyzing certain proteins by controlling the hydrolysis degree and the hydrolysis conditions, and the bioactive polypeptides not only have the advantages of high activity, easy digestion and absorption, high safety and the like, but also have special physiological regulation functions, such as reducing blood fat, delaying senility, beautifying the body and beautifying the face, resisting oxidation, resisting melancholy, resisting fatigue, improving sleep, enhancing memory, inhibiting tumors and the like; can also promote the absorption of protein, vitamins and various beneficial trace elements by human body. Scientific research finds that active polypeptide in an organism is an important chemical messenger communicating cells and organs, and transmits various specific information through action modes such as endocrine, paracrine, neuroendocrine and neuroendocrine, so that life processes such as growth, development, reproduction, metabolism and behavior can be regulated.

The research field of bioactive polypeptide is developed quickly, has been paid high attention by scientists and governments of various countries, and the bioactive polypeptide is obtained by hydrolyzing or processing food protein, has the unique advantages of rich raw materials, low cost, good safety, convenience for industrial production, good applicability and the like, and is advocated by the science of various countries. The active polypeptide is used as a medicine, and the active action points of the active polypeptide are concentrated on shorter polypeptide chains, so that the effective value and the biological activity of the medicine can be improved, the adverse reaction of non-target active ingredients can be reduced, and the specificity of the active polypeptide is improved.

Disclosure of Invention

Based on the current situation, the invention provides an application mode of the crocodile extract in preparation of an anti-cancer drug or an anti-cancer auxiliary drug, the crocodile extract is crocodile polypeptide, crocodile tissue is heated and softened, then high-temperature constant-temperature hydrolysis is carried out, and then hydrolysate is concentrated and dried to obtain crocodile polypeptide extract powder.

The invention achieves the above purpose through the following detailed technical scheme:

an application of crocodile extract in preparing anticancer medicine or anticancer adjuvant medicine is provided.

Wherein the crocodile extract is one or the combination of crocodile bone polypeptide and crocodile meat polypeptide.

Wherein the crocodile bone polypeptide and the crocodile meat polypeptide are prepared by the following steps:

step S10, taking crocodile tissues, fully cleaning, and heating and hydrolyzing the cleaned crocodile tissues;

step S20, standing and precipitating the hydrolysate obtained in the step S10, and filtering to obtain a filtrate which is a crude extract;

step S30, adding diatomite and active carbon into the crude extract obtained in the step S20, stirring, standing for precipitation again, and filtering to obtain filtrate as a fine extract;

step S40, drying the refined extract obtained in step S30 to obtain crocodile peptide powder;

the crocodile tissue is crocodile bone or crocodile meat.

Wherein, the heating hydrolysis in the step S10 is to put the crocodile tissue into distilled water and heat and stir the crocodile tissue in an oil bath kettle at the temperature of 110-130 ℃ for 7-9 h; the mass ratio of the crocodile tissues to the distilled water is that the crocodile tissues: distilled water 1: 5.

wherein the step S10 further comprises a step S11 of soaking crocodile tissues in clear water at 70-80 ℃ for 25-35 min.

Wherein, the step S40 includes a step S41, the refined extract obtained in the step S30 is taken and concentrated by a vacuum water pump to obtain a concentrated extract.

Wherein, the step S40 includes a step S42, and the concentrated extract obtained in the step S41 is dried into powder by using a freeze dryer.

The invention has the following beneficial effects: the crocodile polypeptide has certain activity in the aspects of inhibiting cancers and resisting tumors, and the crocodile polypeptide prepared by the method can be applied to the preparation of anti-cancer drugs, so that the preparation cost of the drugs can be reduced.

Detailed Description

The present invention will be described in detail with reference to specific examples.