阅读说明：本技术 一种治疗母畜子宫炎症的中药灌注制剂配方及制备方法 (Formula and preparation method of traditional Chinese medicine perfusion preparation for treating female livestock uterine inflammation ) 是由 张同金 王慧姣 于 2020-05-09 设计创作，主要内容包括：本发明涉及一种治疗母畜子宫炎症的中药灌注制剂配方及制备方法,包括以下重量份数的成分：横经席130-180份、金樱根130-180份、金银花120-170份、鸡血藤130-180份、红花120-170份、蒲公英120-170份、五指毛桃130-180份、益母草120-170份、苦参80-120份,制作方包括制作横经席、金樱根、鸡血藤、五指毛桃药液1,制作益母草、苦参、红花的药液2,制作蒲公英和金银花的药液3,将三种药液混合、浓缩、注水及得,本发明替代西药抗生素等治疗奶牛子宫内膜炎的理想药品,不但临床效果良好,而且使用方便、操作简单,又可解决化学药品的残留问题,并解决同类中药的治疗不理想的问题。(The invention relates to a formula and a preparation method of a traditional Chinese medicine perfusion preparation for treating female livestock uterine inflammation, wherein the traditional Chinese medicine perfusion preparation comprises the following components in parts by weight: the preparation method comprises the steps of preparing transverse channel mat, cherokee rose root 180, cherokee rose root 130 180, honeysuckle flower 120 170, spatholobus stem 130 180, safflower 120 170, dandelion 120 170, hispid fig 130 180, motherwort 120 170 and sophora flavescens 80-120, wherein the preparation method comprises the steps of preparing transverse channel mat, cherokee rose root, spatholobus stem and hispid fig liquid medicine 1, preparing motherwort herb, sophora flavescens and safflower liquid medicine 2, preparing dandelion and honeysuckle liquid medicine 3, mixing, concentrating and injecting water to obtain the medicinal liquid.)

1. A traditional Chinese medicine perfusion preparation formula for treating female livestock uterine inflammation is characterized in that: the paint comprises the following components in parts by weight: 180 parts of radix Callicarpae Membranacei, 180 parts of cherokee rose root, 120 parts of honeysuckle flower, 180 parts of suberect spatholobus stem, 120 parts of safflower, 120 parts of dandelion, 180 parts of radix fici simplicissimae, 120 parts of motherwort and 80-120 parts of radix sophorae flavescentis.

2. The traditional Chinese medicine perfusion preparation formula for treating female livestock uterine inflammation according to claim 1, which is characterized in that: the paint comprises the following components in parts by weight: 150 portions of transverse channel mat, 150 portions of cherokee rose root, 170 portions of honeysuckle flower, 130 portions of honeysuckle flower, 150 portions of suberect spatholobus stem, 130 portions of safflower, 130 portions of dandelion, 150 portions of hispid fig, 160 portions of motherwort, and 90 portions to 110 portions of lightyellow sophora root.

3. The traditional Chinese medicine perfusion preparation formula for treating female livestock uterine inflammation according to claim 1, which is characterized in that: the paint comprises the following components in parts by weight: 160 parts of radix Calophylli Membranacei, 160 parts of cherokee rose root, 150 parts of honeysuckle, 160 parts of suberect spatholobus stem, 150 parts of safflower, 150 parts of dandelion, 160 parts of hispid Fig, 150 parts of motherwort and 100 parts of radix sophorae flavescentis.

4. A preparation method of a traditional Chinese medicine perfusion preparation for treating female livestock uterine inflammation is characterized by comprising the following steps: the method comprises the following steps:

s1: weighing the parts of the transverse menstruation mat, the cherokee rose root, the suberect spatholobus stem and the hispid fig root, adding diluted ethanol (50%) which is 12 times of the amount of the crude drugs into the mixture, performing reflux extraction in water bath for 2 times, extracting for 2 hours each time, filtering the extracting solution, combining the filtrates, performing reduced pressure concentration, recovering ethanol until no alcohol smell exists, and cooling the filtrate to obtain a liquid medicine 1;

s2: weighing the motherwort and the lightyellow sophora root in parts by weight, cutting, adding 10 times of water together with the safflower in parts by weight, decocting for two times, wherein the first time is 1.5 hours, the second time is 1 hour, combining decoction liquids, filtering, concentrating filtrate to about 400ml, cooling, slowly adding 95% ethanol to ensure that the ethanol precipitation concentration reaches 70%, uniformly stirring, hermetically standing for 24 hours, precipitating, filtering, washing filter residues with 70% ethanol, combining washing liquor and filtrate, recovering ethanol under reduced pressure until no ethanol smell exists, cooling to obtain a liquid medicine 2;

s3: through an L9(34) orthogonal experiment, the preferable result is that the dandelion and the honeysuckle are weighed and added with 8 times of water for decocting and extracting for 2 times, each time is 1 hour, the decoction is combined, the decoction is filtered, the mixture is centrifuged at 2000r/min for 10min, and the supernatant is taken to obtain a liquid medicine 3;

s4: mixing the medicinal liquid 1, 2 and 3, filtering, concentrating, removing impurities, and adding injectable water to desired amount.

Technical Field

The invention relates to a traditional Chinese veterinary medicine preparation, in particular to a traditional Chinese medicine perfusion preparation formula for treating female livestock uterus inflammation and a preparation method thereof.

Background

The prior art comprises the following steps:

1. western medicine (antibiotic) therapy

Researchers and clinical staff at home and abroad do a lot of work for researching and exploring the treatment method of the cow endometritis, and a plurality of effective treatment measures are provided. The measures taken in general are systemic treatment and treatment against local infection of the uterus. Systemic treatment mainly comprises oral administration or intramuscular injection of antibacterial drugs or hormones. However, when the medicine is applied systemically, the medicine in the local part of the uterus is difficult to reach or maintain effective concentration, and the use of a large amount of antibiotic can cause the residual quantity of the antibiotic in blood and milk products to exceed the standard. The topical treatment is mainly to irrigate the uterus and infuse the uterus with bioactive substances like antibacterial drugs or hormones, which are commonly used methods for intrauterine treatment.

Firstly, a patent CN200710072144.8 with the patent name of 'a compound perfusion agent for treating cow endometritis and a preparation method thereof' relates to a formula proportioning of the compound perfusion agent, wherein the content of each 100g of medicinal powder is 30-50 parts of terramycin, 30-50 parts of beta-cyclodextrin, 0.5-4 parts of sodium dodecyl sulfate and 4-10 parts of polyvinylpyrrolidone; every 100g of the medicinal powder comprises 80-120 g of motherwort, 40-60 g of peach kernel, 40-120 g of angelica, 20-40 g of ligusticum wallichii, 40-120 g of astragalus and 40-60 g of liquorice.

The method contains chemical components, has certain residue on the treatment of the dairy cow, and is not beneficial to long-term application.

② a journal article 'curative effect observation of preventing and treating cow endometritis by cervicitis embolism extinguishing suppository' relates to the observation of preventing and treating effect of 90 cows endometritis in dairy farms of the dairy branch of the Yikang industry by cervicitis embolism extinguishing (a traditional Chinese medicine suppository consisting of eucalyptus globulus oil, motherwort, safflower, peach kernel, cattail pollen and the like) and rivanol oxytetracycline solution grouping. Clinical pharmacodynamics shows that in the prevention and contrast test, the effective rate of the test group A is 87.5 percent of that of the multiparous cows.

The test paper observes the effect of preventing and treating the endometritis of 90 cows in the animal farm of the dairy branch company of Yikang by using cervicitis eliminating suppository (a traditional Chinese medicine suppository consisting of eucalyptus globulus oil, motherwort, safflower, peach kernel, cattail pollen and the like) and rivanol oxytetracycline solution in groups. Clinical pharmacodynamics shows that in a prevention contrast test, the effective rate of the test group A is 87.5 percent of multiparous cows and 100 percent of young cows; the conception time is averagely advanced, the number of multiparous cows is 9.6 days, and the number of young cows is 7.5 days. The results show that test group a is slightly more effective than test group B. In the treatment control experiment, the cure rate of the test group A is 66.7%, the remission rate is 16.7%, the cure rate of the test group B is 60.0%, the remission rate is 20.0%, and the cure rate of the test group A is slightly better than that of the test group B.

The traditional Chinese medicine composition contains chemical drugs such as oxytetracycline, can cause the defect of drug residue problem to dairy cows, and has low cure rate in technical performance.

The medicine contains Western medicines such as oxytetracycline and antibiotic components for treating endometritis, can damage uterus after uterus perfusion, and has medicine residues after being absorbed in milk. At present, the national strict requirements on the medicine use of animals apply chemical medicines strictly in the milk producing period, or the medicine stopping period is required, namely the milk in the treatment period is discarded in the milk producing period, so that the defect of medicine residue problem is caused to the dairy cows.

2. Treatment with traditional Chinese medicines:

the antibiotic is easy to cause drug residue on milk source and the drug-resistant strains of the antibiotic and chemical drug are continuously generated, so that the curative effect is reduced. Therefore, in recent ten years, researches on treatment of endometritis of dairy cows by using Chinese herbal medicines are increasing, and a prescription with a lot of experiences is generated. And through scientific experiments, a plurality of Chinese herbal medicine formulas with definite curative effects on the endometritis of the cows are obtained, and a plurality of preparations for treating the endometritis of the cows appear in the market. The Chinese herbal medicine has the obvious functions of resisting bacteria and diminishing inflammation, improving blood circulation and enhancing uterine contraction. At present, more traditional Chinese medicine preparations and curative effects for treating bovine endometritis are reported.

The compound Chinese medicine perfusate for preventing and treating cow endometritis consists of cnidium fruit, flavescent sophora root, coptis root and licorice. The traditional Chinese medicine has a not obvious and not obvious treatment effect.

② a womb clearing and pregnancy promoting perfusion fluid which is a traditional Chinese medicine preparation for preventing and treating endometritis of female animals, relating to a womb clearing and pregnancy promoting perfusion fluid system, and a sterile hypertonic solution is prepared by scientific extraction and development by using a plurality of traditional Chinese medicines of climbing groundsel herb, motherwort herb, Chinese angelica and the like which have the efficacies of clearing away heat and toxic material, promoting blood circulation to remove blood stasis, removing putrefaction and expelling pus, and cleaning womb and promoting pregnancy as raw materials according to the theory of Chinese veterinary medicine. Clinical trials show that: the secondary cure rate of endometritis of female animals by the uterine clearing and pregnancy promoting perfusate is 96.2 percent, wherein the cure rate of the first ytterbium is 86.5 percent. The cure rate is lower in technical performance.

③ medicament for treating cow endometritis and preparation method thereof

The literature: lanzhou livestock and veterinary research institute (Miao Xiao building; Yangling; Supeng; Pan tiger; Wang Yu; Job Zealand, Li jin Yu)

A pharmaceutical composition for treating cow endometritis and its preparation method are provided. Motherwort, poplar flower, hop and echinacea are selected and subjected to ultrasonic extraction and impurity removal by a clarifying agent to obtain clear and transparent reddish brown liquid. The total effective rate of the traditional Chinese medicine for treating the endometritis of the cow is 91.3% for treating 180 cases of the endometritis of the cow, the endometritis of the cow is cured for 3-5 times, and the conception rate in 3 cases after the cure is 80.91%. The traditional Chinese medicine combination treatment method has certain effect, long medication time and lower cure rate and conception rate.

The invention relates to a compound traditional Chinese medicine perfusate for preventing and treating cow endometritis, which can be used for preventing and treating cow endometritis and other diseases caused by bacteria, fungi and trichomonas, and belongs to the field of veterinary medicines. The invention also discloses a preparation method of the compound traditional Chinese medicine perfusion fluid, and the preparation method has a simple preparation process. The traditional Chinese medicine has the defects of unobvious and not obvious treatment effect, and the like.

The traditional Chinese medicine perfusate for preventing and treating female animal infertility is only one safflower perfusion agent (the formula of the traditional Chinese medicine perfusate is composed of 3 traditional Chinese medicines such as epimedium, motherwort, safflower and the like) in the prior traditional Chinese medicine treatment method, and the traditional Chinese medicine combination treatment method has certain effect on pregnancy promotion but is not obvious on treating and repairing inflammation. Can be used for a long time, and has low cure rate and conception rate.

Disclosure of Invention

The invention aims to provide a formula and a preparation method of a traditional Chinese medicine perfusion preparation for treating female livestock uterine inflammation.

The purpose of the invention is realized by the following technical scheme:

a traditional Chinese medicine perfusion preparation formula for treating female livestock uterine inflammation comprises the following components in parts by weight: 180 parts of radix Callicarpae Membranacei, 180 parts of cherokee rose root, 120 parts of honeysuckle flower, 180 parts of suberect spatholobus stem, 120 parts of safflower, 120 parts of dandelion, 180 parts of radix fici simplicissimae, 120 parts of motherwort and 80-120 parts of radix sophorae flavescentis.

In a preferred embodiment, the composition comprises the following components in parts by weight: 150 portions of transverse channel mat, 150 portions of cherokee rose root, 170 portions of honeysuckle flower, 130 portions of honeysuckle flower, 150 portions of suberect spatholobus stem, 130 portions of safflower, 130 portions of dandelion, 150 portions of hispid fig, 160 portions of motherwort, and 90 portions to 110 portions of lightyellow sophora root.

In a preferred embodiment, the composition comprises the following components in parts by weight: 160 parts of radix Calophylli Membranacei, 160 parts of cherokee rose root, 150 parts of honeysuckle, 160 parts of suberect spatholobus stem, 150 parts of safflower, 150 parts of dandelion, 160 parts of hispid Fig, 150 parts of motherwort and 100 parts of radix sophorae flavescentis.

A preparation method of a traditional Chinese medicine perfusion preparation for treating female livestock uterine inflammation is characterized by comprising the following steps: the method comprises the following steps:

s1: weighing the parts of the transverse menstruation mat, the cherokee rose root, the suberect spatholobus stem and the hispid fig root, adding diluted ethanol (50%) which is 12 times of the amount of the crude drugs into the mixture, performing reflux extraction in water bath for 2 times, extracting for 2 hours each time, filtering the extracting solution, combining the filtrates, performing reduced pressure concentration, recovering ethanol until no alcohol smell exists, and cooling the filtrate to obtain a liquid medicine 1; the main effective components of the radix Calophylli Membranacei, radix Rosae Laevigatae, caulis Spatholobi, and hispid Fig are flavonoids. The properties are similar, and the ethanol is easily dissolved in ethanol, so ethanol with a certain concentration is adopted for reflux extraction, and through an L9(34) orthogonal experiment, the result is extracted twice by 50% ethanol, and the effect is the best in two hours each time;

s2: weighing the motherwort and the lightyellow sophora root in parts by weight, cutting, adding 10 times of water together with the safflower in parts by weight, decocting for two times, wherein the first time is 1.5 hours, the second time is 1 hour, combining decoction liquids, filtering, concentrating filtrate to about 400ml, cooling, slowly adding 95% ethanol to ensure that the ethanol precipitation concentration reaches 70%, uniformly stirring, hermetically standing for 24 hours, precipitating, filtering, washing filter residues with 70% ethanol, combining washing liquor and filtrate, recovering ethanol under reduced pressure until no ethanol smell exists, cooling to obtain a liquid medicine 2; the main active ingredients contained in the motherwort, the sophora flavescens and the safflower are alkaloid, safflower yellow, carthamin and the like, and the traditional water extraction and alcohol precipitation process can be adopted for extraction according to the properties and experiences of the ingredients;

s3: through an L9(34) orthogonal experiment, the preferable result is that the dandelion and the honeysuckle are weighed and added with 8 times of water for decocting and extracting for 2 times, each time is 1 hour, the decoction is combined, the decoction is filtered, the mixture is centrifuged at 2000r/min for 10min, and the supernatant is taken to obtain a liquid medicine 3; the dandelion and the honeysuckle both contain chlorogenic acid and are main effective components for resisting bacteria and diminishing inflammation, and considering that the chlorogenic acid has certain sensitivity to heat, the report shows that the content of the chlorogenic acid in the preparation can be reduced along with the increase of the decocting times and the extension of the heating time, so that the extraction processes of the two medicines are considered independently in the experiment, the water extraction process is optimized by an L9(34) orthogonal experiment method, the water extraction process route of the dandelion and the honeysuckle is finally determined through the comprehensive analysis of the results, and the optimal parameters are determined to reduce the sensitivity of the chlorogenic acid which is the main component for resisting bacteria and diminishing inflammation to heat;

s4: mixing the medicinal liquid 1, 2 and 3, filtering, concentrating, removing impurities, and adding injectable water to desired amount.

The clinical curative effect is compared with the curative effects of antibiotics and the like, and the effects have a large difference. The difference lies in that the milk yield of the dairy cow is not affected during the treatment period of the traditional Chinese medicine, the traditional Chinese medicine does not need a withdrawal period, and has the effects of removing blood stasis and relieving pain, promoting blood circulation and stimulating the menstrual flow, promoting blood circulation and removing blood stasis, promoting estrus and promoting pregnancy, sterilizing and diminishing inflammation, promoting mucosa repair, promoting self-cleaning of uterus and improving the intrauterine environment. It can be used for treating acute and chronic endometritis, suppurative endometritis, and puerperal uterine infection caused by retained placenta. Uterine inflammation, cervicitis, irregular oestrus cycle, even no oestrus, female animal infertility and the like.

The invention has the beneficial effects that:

1. the traditional Chinese medicine composition can replace ideal medicines such as western medicine antibiotics and the like for treating the endometritis of the milk cow, has good clinical effect, convenient use and simple operation, can solve the problem of chemical medicine residue and the problem of non-ideal treatment of the similar traditional Chinese medicines, and has the technical performance index cure rate of treating the endometritis of the milk cow of more than 98.7 percent;

2. the cure rate of the technical performance index is more than 98.7 percent, the cure rate for treating the endometritis of the dairy cow is 98.7 percent, the effective rate is 99.7 percent, and most of the endometritis is cured for 1 to 3 times. The conception rate in 3 estrus after treatment was 95.3%. The invention not only can effectively treat endometritis, but also has good effect on improving the conception rate.

Drawings

The invention is explained in further detail below with reference to the drawing.

FIG. 1-1 is a UV absorption spectrum of a control according to an embodiment of the present invention;

FIGS. 1-2 are graphs showing the relationship between the concentration and the absorbance of chlorogenic acid according to examples of the present invention;

FIG. 2 is a high performance liquid chromatogram of chlorogenic acid standard with a concentration of 8.6. mu.g/ml according to an embodiment of the present invention;

FIG. 3 is a graph of the concentration of a chlorogenic acid standard solution versus peak height according to an embodiment of the present invention;

FIG. 4 is a high performance liquid chromatogram of a water extract sample 2 of Taraxacum mongolicum, according to an embodiment of the present invention.

Detailed Description

The invention will now be further described with reference to specific examples.

The invention provides a formula and a preparation method of a traditional Chinese medicine perfusion preparation for treating female livestock uterine inflammation.