阅读说明：本技术 一种具有二次消毒作用的消毒擦剂及其制备方法 (Disinfectant liniment with secondary disinfection effect and preparation method thereof ) 是由 吴康平 于 2020-05-08 设计创作，主要内容包括：本发明公开了一种具有二次消毒作用的消毒擦剂及其制备方法,消毒擦剂包括：聚乙烯醇热轧无纺布线性结构络合的含氯抗菌剂,浓度为60-100％乙醇；聚乙烯醇热轧无纺布线性结构络合的含氯抗菌剂的配方包括：聚乙烯醇0588 15-21份,聚乙烯醇1788 4-10份,含氯抗菌剂2-5份,碳酸钙4份,尿素1.7份,二甲基亚砜64.3-67.3份；本发明先利用乙醇的作用对皮肤、医疗器具或仪器的消毒；乙醇消毒完毕后,将残留的乙醇随主材一起投入到需要消毒的水中,含氯消毒剂从主材料中释放,从而对水中的微生物进行杀灭；本发明实现二次消毒作用,一片产品可以使用的范围广,节能环保。(The invention discloses a disinfectant liniment with secondary disinfection effect and a preparation method thereof, wherein the disinfectant liniment comprises the following components: the polyvinyl alcohol hot-rolled non-woven fabric is a chlorine-containing antibacterial agent which is complexed in a linear structure and has the concentration of 60-100% ethanol; the formula of the chlorine-containing antibacterial agent complexed with the linear structure of the polyvinyl alcohol hot-rolled non-woven fabric comprises: 058815-21 parts of polyvinyl alcohol, 17884-10 parts of polyvinyl alcohol, 2-5 parts of chlorine-containing antibacterial agent, 4 parts of calcium carbonate, 1.7 parts of urea and 64.3-67.3 parts of dimethyl sulfoxide; the invention firstly utilizes the function of ethanol to disinfect skin, medical appliances or instruments; after the ethanol disinfection is finished, the residual ethanol is put into the water to be disinfected along with the main material, and the chlorine-containing disinfectant is released from the main material, so that the microorganisms in the water are killed; the invention realizes secondary disinfection, and the product has wide application range, energy saving and environmental protection.)

1. A disinfectant liniment with secondary disinfection effect is characterized by comprising: the polyvinyl alcohol hot-rolled non-woven fabric is a chlorine-containing antibacterial agent which is complexed in a linear structure and has the concentration of 60-100% ethanol; the formula of the chlorine-containing antibacterial agent complexed in the linear structure of the polyvinyl alcohol hot-rolled non-woven fabric comprises: 058815-21 parts of polyvinyl alcohol, 17884-10 parts of polyvinyl alcohol, 2-5 parts of chlorine-containing antibacterial agent, 4 parts of calcium carbonate, 1.7 parts of urea and 64.3-67.3 parts of dimethyl sulfoxide.

2. A disinfecting liniment with secondary disinfecting effect as claimed in claim 1, wherein the formulation of the chlorine-containing antibacterial agent complexed with the linear structure of the polyvinyl alcohol hot-rolled non-woven fabric comprises: 058818-20 parts of polyvinyl alcohol, 17885-7 parts of polyvinyl alcohol, 2.8-3.5 parts of chlorine-containing antibacterial agent, 4 parts of calcium carbonate, 1.7 parts of urea and 65.8-66.5 parts of dimethyl sulfoxide.

3. A disinfecting liniment with secondary disinfecting effect as claimed in claim 1, wherein the formulation of the chlorine-containing antibacterial agent complexed with the linear structure of the polyvinyl alcohol hot-rolled non-woven fabric comprises: 058819 parts of polyvinyl alcohol, 17886 parts of polyvinyl alcohol, 3.2 parts of chlorine-containing antibacterial agent, 4 parts of calcium carbonate, 1.7 parts of urea and 66.1 parts of dimethyl sulfoxide.

4. A disinfecting wipe as claimed in any one of claims 1 to 3, wherein said chlorine-containing antimicrobial agent comprises: hypochlorite, chlorhexidine acetate, chlorhexidine gluconate, benzalkonium chloride, triclosan, sodium dichloroisocyanurate, trichloroisocyanuric acid, ammonium chloride T.

5. A method for preparing a disinfectant liniment with secondary disinfection effect is characterized by comprising the following steps:

step one, preparing a spinning solution according to a formula;

the formula comprises the following components: 058815-21 parts of polyvinyl alcohol, 17884-10 parts of polyvinyl alcohol, 2-5 parts of chlorine-containing antibacterial agent, 4 parts of calcium carbonate, 1.7 parts of urea and 64.3-67.3 parts of dimethyl sulfoxide;

dissolving polyvinyl alcohol by using dimethyl sulfoxide, and then adding benzalkonium chloride, calcium carbonate and urea to obtain spinning stock solution;

step two, preparing PVA fiber by using the spinning solution;

filtering the spinning solution, spraying the spinning solution from a spinneret plate at the temperature of 70-85 ℃, spraying the spinning solution into a methanol solution, solidifying and forming at room temperature, and performing wet stretching to obtain nascent PVA fibers; carrying out dry stretching on the nascent PVA fiber in a heat pipe at the temperature of 200 ℃ to obtain PVA fiber;

step three, hot rolling the non-woven fabric;

cutting, opening, combing, overlapping, hot rolling, coiling and cutting the prepared PVA fiber to form polyvinyl alcohol hot-rolled non-woven fabric;

step four, forming and packaging the liniment;

and injecting 60-100% ethanol into the obtained polyvinyl alcohol hot-rolled non-woven fabric, and packaging.

6. A process for preparing a disinfecting liniment having secondary disinfecting action as claimed in claim 5, wherein the first step is to prepare a spinning dope according to a formulation;

the formula comprises the following components: 058815-21 parts of polyvinyl alcohol, 17884-10 parts of polyvinyl alcohol, 2-5 parts of chlorine-containing antibacterial agent, 4 parts of calcium carbonate, 1.7 parts of urea and 64.3-67.3 parts of dimethyl sulfoxide;

the specific preparation method of the spinning solution comprises the following steps:

firstly, adding polyvinyl alcohol 0588 and polyvinyl alcohol 1788 into a DMSO solution, heating to 80 ℃, and stirring for 2 hours so that the polyvinyl alcohol is fully dissolved;

and secondly, adding the benzalkonium chloride, the calcium carbonate and the urea when the temperature of the dissolved polyvinyl alcohol/dimethyl sulfoxide solution is reduced to 15-35 ℃, and continuously stirring for 30 minutes to prepare a spinning solution.

7. A method for preparing a disinfectant liniment with secondary disinfection effect as claimed in claim 5, wherein in step two, PVA fiber is prepared from spinning solution;

filtering the spinning solution, spraying the spinning solution from a spinneret plate at the temperature of 75-83 ℃, spraying the spinning solution into a methanol solution, solidifying and forming at room temperature, and performing wet stretching to obtain nascent PVA fibers; and (3) carrying out dry stretching on the nascent PVA fiber in a heat pipe at the temperature of 200 ℃ to obtain the PVA fiber.

8. A method for preparing a disinfectant liniment with secondary disinfection effect as claimed in claim 7, wherein in step two, PVA fiber is prepared from spinning solution;

filtering the spinning solution, spraying the spinning solution from a spinneret plate at the temperature of 81 ℃, spraying the spinning solution into a methanol solution, solidifying and forming at room temperature, and performing wet stretching to obtain nascent PVA fibers; and (3) carrying out dry stretching on the nascent PVA fiber in a heat pipe at the temperature of 200 ℃ to obtain the PVA fiber.

9. A process for preparing a disinfecting liniment having secondary disinfecting action as claimed in claim 5, wherein in step four, the liniment is packaged;

and (3) injecting 70-95% ethanol into the obtained polyvinyl alcohol hot-rolled non-woven fabric, and packaging.

10. A process for preparing a disinfecting liniment having secondary disinfecting action as claimed in claim 5, wherein in step four, the liniment is packaged;

loading the polyvinyl alcohol hot-rolled non-woven fabric and the aluminum plastic packaging bag into a guide roller; filling 70-95% ethanol into an injection pump;

adjusting the amount of alcohol injected into the single piece of the raw material, and producing at a production speed of 40-100 pieces/min;

finally, the disinfection liniment product is obtained by heat sealing.

Technical Field

The invention relates to the field of washing and chemical treatment, in particular to a disinfectant liniment with secondary disinfection effect and a preparation method thereof.

Background

Alcohol (ethanol), is commonly used in medicineIs one of the most widely used disinfectants. Most commonly used for non-invasive skin disinfection, and for cleaning and disinfecting object surfaces. Medical alcohol refers to alcohol used in medicine, and the purity of medical alcohol is various, and is usually 95% and 75%. The alcohol molecule has two ends, one of which is hydrophobic (-C)₂H₅) The attractive force between hydrophobic groups in the protein can be destroyed; one end is hydrophilic (-OH), but it is difficult to break the attractive force between hydrophilic groups outside the protein, and alcohol sterilization mainly denatures the protein.

The chlorine-containing disinfectant is mainly applied to sterilization and disinfection of hotels, hospitals and families, and is widely applied to sterilization and algae removal of swimming pools and industrial circulating water and environmental disinfection of livestock farms. The product has unique sterilization characteristics, and can effectively and quickly kill various microorganisms including bacterial propagules, viruses, fungi, tubercle bacillus and bacterial spores with strongest resistance. The chlorine-containing disinfectant with a certain concentration has stable performance, no stimulation to skin and no pollution to environment. Safe and reliable use and good disinfection effect, and is a common necessary product for disinfection in daily life.

The disinfection mechanisms of alcohol and chlorine-containing disinfectants are different, the alcohol disinfectant and the chlorine-containing disinfectants are sometimes used by the market respectively in order to enhance the disinfection effect, the alcohol disinfectant sold in the market can only erase viruses and bacteria on the skin and can not be applied for the second time, and the market needs a product which can disinfect water after being applied to the skin and has the secondary disinfection effect.

Disclosure of Invention

In order to solve the deficiency of the prior art, the invention aims to provide a disinfectant with secondary disinfection function and a preparation method thereof, firstly utilizing the function of ethanol to disinfect skin, medical appliances or instruments; after the ethanol disinfection is finished, the residual ethanol is put into the water to be disinfected along with the main material, the water dilutes the ethanol concentration, the main material starts to disintegrate and dissolve under the action of the water, and the chlorine-containing disinfectant is released from the main material, so that the microorganisms in the water are killed; the secondary disinfection effect is realized, and the range of application of one product is wide, energy is saved, and the environment is protected.

In order to achieve the above object, the present invention adopts the following technical solutions:

a disinfectant liniment with secondary disinfection effect comprises: the polyvinyl alcohol hot-rolled non-woven fabric is a chlorine-containing antibacterial agent which is complexed in a linear structure and has the concentration of 60-100% ethanol; the formula of the chlorine-containing antibacterial agent complexed with the linear structure of the polyvinyl alcohol hot-rolled non-woven fabric comprises: 058815-21 parts of polyvinyl alcohol, 17884-10 parts of polyvinyl alcohol, 2-5 parts of chlorine-containing antibacterial agent, 4 parts of calcium carbonate, 1.7 parts of urea and 64.3-67.3 parts of dimethyl sulfoxide.

The formula of the chlorine-containing antibacterial agent complexed in the linear structure of the polyvinyl alcohol hot-rolled non-woven fabric comprises the following components: 058818-20 parts of polyvinyl alcohol, 17885-7 parts of polyvinyl alcohol, 2.8-3.5 parts of chlorine-containing antibacterial agent, 4 parts of calcium carbonate, 1.7 parts of urea and 65.8-66.5 parts of dimethyl sulfoxide.

The formula of the chlorine-containing antibacterial agent complexed in the linear structure of the polyvinyl alcohol hot-rolled non-woven fabric comprises the following components: 058819 parts of polyvinyl alcohol, 17886 parts of polyvinyl alcohol, 3.2 parts of chlorine-containing antibacterial agent, 4 parts of calcium carbonate, 1.7 parts of urea and 66.1 parts of dimethyl sulfoxide.

The disinfectant liniment with secondary disinfection function comprises the following chlorine-containing antibacterial agents: hypochlorite, chlorhexidine acetate, chlorhexidine gluconate, benzalkonium chloride, triclosan, sodium dichloroisocyanurate, trichloroisocyanuric acid, ammonium chloride T.

A method for preparing disinfectant liniment with secondary disinfection effect comprises the following steps:

step one, preparing a spinning solution according to a formula;

the formula comprises the following components: 058815-21 parts of polyvinyl alcohol, 17884-10 parts of polyvinyl alcohol, 2-5 parts of chlorine-containing antibacterial agent, 4 parts of calcium carbonate, 1.7 parts of urea and 64.3-67.3 parts of dimethyl sulfoxide;

firstly, dissolving polyvinyl alcohol by using dimethyl sulfoxide, and then adding a chlorine-containing antibacterial agent, calcium carbonate and urea to obtain a spinning stock solution;

step two, preparing PVA fiber by using the spinning solution;

filtering the spinning solution, spraying the spinning solution from a spinneret plate at the temperature of 70-85 ℃, spraying the spinning solution into a methanol solution, solidifying and forming at room temperature, and performing wet stretching to obtain nascent PVA fibers; carrying out dry stretching on the nascent PVA fiber in a heat pipe at the temperature of 200 ℃ to obtain PVA fiber;

step three, hot rolling the non-woven fabric;

cutting, opening, combing, overlapping, hot rolling, coiling and cutting the prepared PVA fiber to form polyvinyl alcohol hot-rolled non-woven fabric;

step four, forming and packaging the liniment;

and injecting 60-100% ethanol into the obtained polyvinyl alcohol hot-rolled non-woven fabric, and packaging.

In the preparation method of the disinfectant liniment with secondary disinfection function,

step one, preparing a spinning solution according to a formula;

the formula comprises the following components: 058815-21 parts of polyvinyl alcohol, 17884-10 parts of polyvinyl alcohol, 2-5 parts of chlorine-containing antibacterial agent, 4 parts of calcium carbonate, 1.7 parts of urea and 64.3-67.3 parts of dimethyl sulfoxide;

the specific preparation method of the spinning solution comprises the following steps:

firstly, adding polyvinyl alcohol 0588 and polyvinyl alcohol 1788 into a DMSO solution, heating to 80 ℃, and stirring for 2 hours so that the polyvinyl alcohol is fully dissolved;

and secondly, when the temperature of the dissolved polyvinyl alcohol/dimethyl sulfoxide solution is reduced to 15-35 ℃, adding the chlorine-containing antibacterial agent, calcium carbonate and urea, and continuously stirring for 30 minutes to prepare the spinning solution.

In the preparation method of the disinfectant liniment with secondary disinfection function,

step two, preparing PVA fiber by using the spinning solution;

filtering the spinning solution, spraying the spinning solution from a spinneret plate at the temperature of 75-83 ℃, spraying the spinning solution into a methanol solution, solidifying and forming at room temperature, and performing wet stretching to obtain nascent PVA fibers; and (3) carrying out dry stretching on the nascent PVA fiber in a heat pipe at the temperature of 200 ℃ to obtain the PVA fiber.

In the preparation method of the disinfectant liniment with secondary disinfection function,

step two, preparing PVA fiber by using the spinning solution;

filtering the spinning solution, spraying the spinning solution from a spinneret plate at the temperature of 81 ℃, spraying the spinning solution into a methanol solution, solidifying and forming at room temperature, and performing wet stretching to obtain nascent PVA fibers; and (3) carrying out dry stretching on the nascent PVA fiber in a heat pipe at the temperature of 200 ℃ to obtain the PVA fiber.

In the preparation method of the disinfectant liniment with secondary disinfection function,

step four, forming and packaging the liniment;

and (3) injecting 70-95% ethanol into the obtained polyvinyl alcohol hot-rolled non-woven fabric, and packaging.

In the preparation method of the disinfectant liniment with secondary disinfection function,

step four, forming and packaging the liniment;

loading the polyvinyl alcohol hot-rolled non-woven fabric and the aluminum plastic packaging bag into a guide roller; filling 70-95% ethanol into an injection pump;

adjusting the amount of alcohol injected into the single piece of the raw material, and producing at a production speed of 40-100 pieces/min;

finally, the disinfection liniment product is obtained by heat sealing.

In the preparation method of the disinfectant liniment with secondary disinfection function,

step four, forming and packaging the liniment;

loading the polyvinyl alcohol hot-rolled non-woven fabric and the aluminum plastic packaging bag into a guide roller; filling 70-95% ethanol into an injection pump;

adjusting the amount of alcohol injected into the single piece of the raw material, and producing at a production speed of 50-70 pieces/min;

finally, the disinfection liniment product is obtained by heat sealing.

The invention has the advantages that:

polyvinyl alcohol is rapidly molded in dimethyl sulfoxide solvent, and after methanol coagulation bath in later period, the curing effect is good;

the methanol can be used as a coagulating bath of the spinning solution and can also be used as an extractant of dimethyl sulfoxide;

the generated polyvinyl alcohol hot-rolled non-woven fabric with a linear structure is used for complexing and fixing the chlorine-containing antibacterial agent, and the chlorine-containing antibacterial agent stably exists in the system;

through practical tests, after the polyvinyl alcohol 1799 is added, the final product cannot be completely dissolved in water due to the high alcoholysis degree of 1799. If the polyvinyl alcohol with the mark of 1788 is completely adopted, the molecular weight is large, the water solubility is relatively poor, and the disintegration is facilitated; if the polyvinyl alcohol 0588 is completely adopted, the disintegration speed is high, but the mechanical property of the material is poor; therefore, the polyvinyl alcohol resin compounded by the marks 1788 and 0588 is used as the raw material of the spinning stock solution, and the good mechanical property can be kept while the spinning stock solution is rapidly disintegrated;

the fillers such as calcium carbonate and the like destroy intermolecular force of polyvinyl alcohol, so that the dispersion speed of the polyvinyl alcohol in water is improved, and the dissolution of the polyvinyl alcohol in water is accelerated;

because inorganic chlorine and organic chlorine are easy to deliquesce, the properties after deliquescence are unstable; the chlorine-containing disinfectant is dispersed in the interface of the main material, and the activity of the chlorine-containing disinfectant is limited to a certain extent and the property is stable because the main material is solid, so that the effective period of the product is prolonged;

the ethanol solution with the concentration of more than 60 percent has certain repulsion to the main material due to the group of ethanol-C2H 5, and shows that the ethanol solution has protective effect on the main material; the chlorine-containing disinfectant contained in the main material is more stable due to the protective effect of the ethanol on the main material.

When in disinfection, the main material is soaked with ethanol, and the effect of the ethanol is utilized firstly, so that the disinfectant can be used for disinfecting hands and skin, and can also be used for disinfecting the surfaces of medical instruments and precision instruments such as thermometers, sphygmomanometers and the like;

after the ethanol disinfection is finished, the residual ethanol is put into the water to be disinfected together with the main material, the water dilutes the ethanol concentration, the main material starts to disintegrate and dissolve under the action of the water, and the chlorine-containing disinfectant is released from the main material, so that the microorganisms in the water are killed.

Drawings

FIG. 1 shows the area shrinkage of the hot-rolled polyvinyl alcohol nonwoven fabric in ethanol solutions with different volume fractions;

fig. 2 is a schematic illustration of a package according to an embodiment of the present invention.

The meaning of the reference symbols in the figures:

1 aluminum foil bag, 2 sealing parts, 3 hot-rolled non-woven fabric and alcohol, and 4 easy-to-tear cuts.

Detailed Description

The invention is described in detail below with reference to the figures and the embodiments.

A disinfectant liniment with secondary disinfection effect comprises: the polyvinyl alcohol hot-rolled non-woven fabric is a chlorine-containing antibacterial agent which is complexed in a linear structure and has the concentration of 60-100% ethanol; the formula of the chlorine-containing antibacterial agent complexed with the linear structure of the polyvinyl alcohol hot-rolled non-woven fabric comprises: 058815-21 parts of polyvinyl alcohol, 17884-10 parts of polyvinyl alcohol, 2-5 parts of benzalkonium chloride, 4 parts of calcium carbonate, 1.7 parts of urea and 64.3-67.3 parts of dimethyl sulfoxide.

A method for preparing disinfectant liniment with secondary disinfection effect comprises the following steps:

step one, preparing a spinning solution according to a formula;

the formula comprises the following components: 058815-21 parts of polyvinyl alcohol, 17884-10 parts of polyvinyl alcohol, 2-5 parts of benzalkonium chloride, 4 parts of calcium carbonate, 1.7 parts of urea and 64.3-67.3 parts of dimethyl sulfoxide;

the specific preparation method of the spinning solution comprises the following steps:

firstly, adding polyvinyl alcohol 0588 and polyvinyl alcohol 1788 into a DMSO solution, heating to 80 ℃, and stirring for 2 hours so that the polyvinyl alcohol is fully dissolved;

and secondly, adding the benzalkonium chloride, the calcium carbonate and the urea when the temperature of the dissolved polyvinyl alcohol/dimethyl sulfoxide solution is reduced to 15-35 ℃, and continuously stirring for 30 minutes to prepare a spinning solution.

Step two, preparing PVA fiber by using the spinning solution;

filtering the spinning solution, spraying the spinning solution from a spinneret plate at the temperature of 70-85 ℃, spraying the spinning solution into a methanol solution, solidifying and forming at room temperature, and performing wet stretching to obtain nascent PVA fibers; and (3) carrying out dry stretching on the nascent PVA fiber in a heat pipe at the temperature of 200 ℃ to obtain the PVA fiber. The spinning dope discharge temperature is preferably in the range of 75 to 83 ℃ and more preferably 81 ℃.

Step three, hot rolling the non-woven fabric;

and cutting, opening, combing, overlapping, hot rolling, reeling and slitting the prepared PVA fiber to form the polyvinyl alcohol hot-rolled non-woven fabric.

Step four, forming and packaging the liniment;

loading the polyvinyl alcohol hot-rolled non-woven fabric and the aluminum plastic packaging bag into a guide roller; filling 60-100% ethanol into an injection pump; adjusting the amount of alcohol injected into the single piece of the raw material, and producing at a production speed of 40-100 pieces/min; finally, the disinfection liniment product is obtained by heat sealing. Preferably, the concentration of ethanol is 70-95%; as a preference, the production speed is 50-70 pieces/min.

The package is made by wrapping hot-rolled non-woven fabrics and alcohol 3 by an aluminum foil bag 1, a sealing part 2 is sealed by heat sealing, and the package is provided with an easy-tearing notch 4.

The preferable conditions of the process parameters are as follows:

item	Variables of	Optimization	Preference is given to
				Spinning dope ejection temperature	70-85℃	75-83℃	81℃
Alcohol concentration	60-100％	70-95％	/
				Production speed	40-100 pieces/min	50-70 pieces/min	55-65 pieces/min

Under the condition of 60-100% ethanol, the chlorine-containing antibacterial agent complexed with the linear structure of the polyvinyl alcohol hot-rolled non-woven fabric is stable due to the coexistence of the hot-rolled non-woven fabric and the alcohol, and the principle is explained as follows: a large number of hydroxyl groups exist on a polyvinyl alcohol polymer chain, the polyvinyl alcohol polymer chain has strong hydrophilicity, and water is a good solvent of the polyvinyl alcohol. The ethyl (-C2H5) in ethanol is used as a hydrophobic alkyl functional group, has certain repulsion to polyvinyl alcohol and is not dissolved in ethanol. The dissolution process of polyvinyl alcohol in water is carried out in stages, namely: affinity wetting-swelling-infinite swelling-dissolution. The experimental result shows (see figure 1) that when the volume fraction of the ethanol is in the range of 0-60%, the polyvinyl alcohol hot-rolled non-woven fabric is swelled and dissolved in the solution; when the volume fraction of ethanol exceeds 60%, the swelling degree of the polyvinyl alcohol hot-rolled non-woven fabric in the solution is reduced, and the volume is shrunk (the advanced chemical bulletin, 2019,40 and 7).

The principle of secondary disinfection is as follows: when the hot-rolled nonwoven fabric containing the chlorine-containing antibacterial agent and ethanol are thrown into water, the hot-rolled nonwoven fabric starts to disintegrate because the ethanol is diluted, and the chlorine-containing disinfectant is released from the main material when the ethanol concentration is diluted to less than 60%.

Samples were made according to the formulation of the following examples:

experimental results of the stable relationship between the polyvinyl alcohol hot-rolled nonwoven fabric and the alcohol concentration: the obtained sample was not stably molded only after swelling the polyvinyl alcohol hot-rolled nonwoven fabric of the comparative example in 55% alcohol.

The samples obtained in the above examples were tested as follows

Detecting the alcohol disinfection effect: hand hygiene experiments;

according to the requirements of medical staff's hand hygiene standard, the alcohol liniment with alcohol is applied to the curved surfaces of hands or fingers from the finger root to the finger tip for 2 times, and the area of the liniment is about 30cm²The cotton swab was placed in a 10mL test tube containing a sterile eluent containing the corresponding neutralizer, and the number of colonies was counted after incubation.

The detection results are as follows:

detecting the disinfection effect of the chlorine-containing disinfectant;

the experimental report of the disinfection performance of the chlorine-containing disinfectant comprises the following steps:

1. the purpose is as follows: and (5) detecting the disinfection performance of the chlorine-containing disinfectant.

2. Experimental instruments/materials: purified water, a common broth culture medium, a nutrient agar culture medium, a Sabouraud agar culture medium, a Staphylococcus aureus CMCC (B)26003, a Pseudomonas aeruginosa CMCC (B)10104, a Bacillus subtilis CMCC (B)63501, a Candida albicans CMCC (F)98001, an Escherichia coli CMCC (B)44103, 0.9% sterile physiological saline, a beaker, a petri dish, forceps, an incubator, a pressure steam sterilizer, a pipette gun, a pipette tip and a test tube.

3. The experimental steps are as follows:

3.1 dissolving 1 g of disinfectant in 50mL of water to prepare a test solution.

3.2 culture Medium common broth culture medium was dispensed into test tubes before use, 2mL per tube, sterilized at 115 ℃ for 30 min; nutrient agar medium, Sabouraud agar medium, sterilized at 115 deg.C for 30min before use.

3.3 Staphylococcus aureus, Pseudomonas aeruginosa, Bacillus subtilis, and Escherichia coli, all of which are dipped from the slant of the preserved strain before the experiment, and inoculated into 10mL nutrient broth culture medium, cultured at 37 deg.C for 24h, and taken out as the original bacteria solution, and then diluted to 1 μ L/L with sterile normal saline for use. Before experiment, Candida albicans is dipped from the slant of the preserved strain, inoculated into 10mL of fungus culture medium, cultured for 72h at 25 ℃, taken out as original bacteria liquid, and diluted to 10 mu L/L by sterile normal saline for later use.

3.4 tube Serial dilution method, take and hold 2mL ordinary broth culture medium or fungus culture medium 12 tubes, arrange on the test-tube rack, number. Then adding 2mL of test solution into the 1 st tube by an aseptic operation method, shaking up, sucking 2mL of test solution, adding into the 2 nd tube, shaking up, diluting to the 10 th tube by the same method, and discarding 2 mL; 0.1mL of prepared solution at a concentration of 1. mu.L/L or 10. mu.L/L was added to each tube. Only 0.1mL of bacterial liquid is added into the 11 th tube to be used as a positive growth control tube; and adding 2mL of test solution into the 12 th tube, uniformly mixing, and discarding 2mL of non-added bacteria solution as a negative control tube. And (3) placing each tube added with staphylococcus aureus, pseudomonas aeruginosa, bacillus subtilis and escherichia coli liquid at 37 ℃ for culturing for 24h, placing each tube added with candida albicans liquid at 25 ℃ for culturing for 48h, and observing the result. After the cultivation, each tube which grows aseptically is dipped and smeared on a common broth agar or a Sabouraud agar plate by a sterilization inoculating ring, and the tube is cultivated at 37 ℃ for 24h (staphylococcus aureus, pseudomonas aeruginosa, bacillus subtilis and escherichia coli) or at 25 ℃ for 72h (candida albicans) and the result is observed.

The results are shown in tables 1-6.

Table 1 example 1 results of experiments on disinfection with chlorine-containing disinfectant

Table 2 example 2 results of experiments on disinfection with chlorine-containing disinfectant

Table 3 example 3 results of experiments on disinfection with chlorine-containing disinfectant

Table 4 example 4 results of experiments on disinfection with chlorine disinfectant

Table 5 example 5 results of experiments on disinfection with chlorine-containing disinfectant

TABLE 6 comparative examples Disinfection test results with chlorine-containing disinfectant

The "+" indicates growth of bacteria, 11 tubes only added with bacteria liquid and no chlorine-containing disinfectant as positive control; "-" indicates no growth of bacteria, and 12 tubes were filled with only chlorine-containing disinfectant and no bacteria solution, as a negative control.

The experimental results show that the chlorine-containing disinfectant has strong bactericidal effects on staphylococcus aureus, pseudomonas aeruginosa, bacillus subtilis, candida albicans and escherichia coli.

The invention firstly utilizes the function of ethanol to disinfect skin, medical appliances or instruments; after the ethanol disinfection is finished, the residual ethanol is put into the water to be disinfected along with the main material, the water dilutes the ethanol concentration, the main material starts to disintegrate and dissolve under the action of the water, and the chlorine-containing disinfectant is released from the main material, so that the microorganisms in the water are killed; the secondary disinfection effect is realized, the range of application of one product is wide, and the energy is saved and the environment is protected; the chlorine-containing disinfectant is dispersed in the interface of the main material, and the activity of the chlorine-containing disinfectant is limited to a certain extent and the property is stable because the main material is solid, so that the effective period of the product is prolonged; the ethanol solution with the concentration of more than 60 percent has certain repulsion to the main material due to the ethanol-C2H 5 group, and shows that the ethanol solution has protective effect on the main material. The chlorine-containing disinfectant contained in the main material is more stable due to the protective effect of the ethanol on the main material.

The foregoing illustrates and describes the principles, general features, and advantages of the present invention. It should be understood by those skilled in the art that the above embodiments do not limit the present invention in any way, and all technical solutions obtained by using equivalent alternatives or equivalent variations fall within the scope of the present invention.