阅读说明：本技术 一种可自动提取pbmc的装置及方法 (Device and method capable of automatically extracting PBMCs ) 是由 毕利军 马文美 杨晓 马道稳 于 2020-05-14 设计创作，主要内容包括：本发明涉及一种可自动提取PBMC的装置及方法,包括如下部件：机架,所述机架包括底座、上支撑板、侧支撑板和侧支架,所述侧支撑板的两端分别与所述底座和所述上支撑板连接,所述侧支架设置在所述侧支撑板上；抽吸机构,设置在所述侧支架上,用于抽吸样品；第一升降机构,设置在所述上支撑板和所述侧支撑板上,其包括第二滑块,可沿所述侧支撑板的高度方向上升或下降；第二升降机构,设置在所述上支撑板和所述侧支撑板上,其包括第三滑块,亦可沿所述侧支撑板的高度方向上升或下降；移液机构,设置在所述第二滑块上用于移取样品。本发明装置结构简单,体积小,能够满足生物医疗器械领域对小而精的移液装置需求。(The invention relates to a device and a method capable of automatically extracting PBMC (peripheral blood mononuclear cell), which comprises the following components: the rack comprises a base, an upper supporting plate, a side supporting plate and a side support, wherein two ends of the side supporting plate are respectively connected with the base and the upper supporting plate, and the side support is arranged on the side supporting plate; the suction mechanism is arranged on the side bracket and is used for sucking a sample; the first lifting mechanism is arranged on the upper supporting plate and the side supporting plate, comprises a second sliding block and can ascend or descend along the height direction of the side supporting plate; the second lifting mechanism is arranged on the upper supporting plate and the side supporting plate, comprises a third sliding block and can also ascend or descend along the height direction of the side supporting plate; and the liquid transferring mechanism is arranged on the second sliding block and used for transferring and taking the sample. The device has simple structure and small volume, and can meet the requirements of the field of biomedical equipment on small and precise liquid transfer devices.)

1. An apparatus for automatically extracting PBMCs, comprising:

the rack (1) comprises a base (101), an upper supporting plate (102), a side supporting plate (103) and a side support (104), wherein two ends of the side supporting plate (103) are respectively connected with the base (101) and the upper supporting plate (102), and the side support (104) is arranged on the side supporting plate (103);

a suction mechanism provided on the side stand (104) for sucking a sample in a sample container;

a first lifting mechanism which is arranged on the upper supporting plate (102) and the side supporting plate (103), comprises a second sliding block (10) and can ascend or descend along the height direction of the side supporting plate (103);

the liquid transferring mechanism is arranged on the second slide block (10) and is used for transferring the sample in the sample container;

the second lifting mechanism is arranged on the upper supporting plate (102) and the side supporting plate (103), comprises a third sliding block (12) and can also ascend or descend along the height direction of the side supporting plate (103);

and the layered liquid level detection mechanism is arranged on the third slide block (12) and is used for detecting the layering condition of the liquid level of the sample.

2. The device according to claim 1, wherein the pumping mechanism comprises a liquid transferring pump (6), the liquid transferring pump (6) comprises a liquid transferring motor (601), a lifting shaft screw (604) and a pumping barrel (602), the output end of the liquid transferring motor (601) is connected with the lifting shaft screw (604), the lifting shaft screw (604) is connected with the lower end of the pumping barrel (602), and a piston (603) is arranged in the pumping barrel (602).

3. The device according to claim 1, wherein the first lifting mechanism further comprises a first lifting motor (3) and a pipetting screw (4), both ends of the pipetting screw (4) are respectively connected with the base (101) and the upper support plate (102), the first lifting motor (3) is arranged on the side support plate (103), the output end of the first lifting motor (3) is connected with the pipetting screw (4) through a belt, the pipetting screw (4) is sleeved with the second slide block (10) which can slide along the axial direction of the pipetting screw, and the second slide block (10) is positioned below the lower end surface of the upper support plate (102).

4. The device according to claim 2, characterized in that the pipetting mechanism comprises a catheter (5) and a first slide (9), the lower end of the first slide (9) being provided with a grip (901), the upper end of the first slide (9) being fixed to the second slide (10) and being connected to the first end of the catheter (5), the second end of the catheter (5) being connected to the upper end of the aspiration cylinder (602).

5. The device according to claim 1, wherein the second lifting mechanism further comprises a second lifting motor (13) and a height measuring screw (7), the second lifting motor (13) is disposed on the side supporting plate (103), two ends of the height measuring screw (7) are respectively connected with the base (101) and the upper supporting plate (102), an output end of the second lifting motor (13) is connected with the height measuring screw (7) through a belt, the height measuring screw (7) is sleeved with the third sliding block (12) which can slide along an axial direction of the height measuring screw, and the third sliding block (12) is located below a lower end face of the upper supporting plate (102).

6. The device according to claim 1, wherein the layered liquid level detection mechanism comprises a fiber optic cassette (11), a first clamping leg (111) and a second clamping leg (112) are arranged at the lower end of the fiber optic cassette (11) and are parallel to the pipetting screw (4), a pair of holes are arranged on the first clamping leg (111) and the second clamping leg (112), and a transmitter and a receiver are respectively arranged in the holes.

7. The device according to claim 6, characterized in that the elevation screw (7) is arranged vertically parallel to the pipetting screw (4).

8. The device according to claim 1, characterized in that two sliding rails (105) are arranged on the side supporting plate (103), and the second slider (10) and the third slider (12) are respectively provided with sliding grooves to match with the sliding rails (105).

9. A method of operating the apparatus of any one of claims 1 to 8, comprising the steps of:

(1) when a sample is sucked, firstly, the second lifting motor (13) is started, the layering condition of the sample is detected through the layering liquid level detection mechanism, then, the first lifting motor (3) is started, the liquid transfer mechanism is driven by the first lifting motor (3) to move downwards to a designated position above a sample tube, finally, the liquid transfer motor (601) is started, the piston (603) moves downwards to generate suction force, the gun head (2) sucks the sample under the action of the suction force, when the sample is sucked, the running speed and the step number of the liquid transfer motor (601) are controlled through the controller (8) to control the liquid suction amount, meanwhile, the descending speed of the gun head (2) is controlled through the controller (8), the gun head (2) is guaranteed to suck downwards at a cell layer face all the time, and accurate cell sucking is realized;

(2) when the sample is discharged, the piston (603) moves upwards to generate thrust, and the pipetting mechanism discharges the sample in the gun head (2) under the action of the thrust.

Technical Field

The invention relates to a device and a method capable of automatically extracting PBMC (peripheral blood mononuclear cell), belonging to the technical field of biomedical equipment.

Background

In vitro determination of the number and function of immune cells often requires that certain immune cells be first isolated. At present, peripheral blood mononuclear cells (pbmcs) are generally separated by a density gradient centrifugation method, and then an experimenter extracts the pbmcs by using a manual pipette gun. The extraction result can be influenced by the level of operators, and the working intensity is high and the efficiency is low.

Disclosure of Invention

Aiming at the outstanding problems, the invention provides a device and a method capable of automatically extracting PBMC (peripheral blood mononuclear cell), wherein the device is additionally provided with a layering liquid level detection mechanism, particularly a sensitive optical fiber detection method, respectively detects the positions of different cell layers according to the different cell layers after layering and the difference of the light transmittance of liquid to light, and drives a suction mechanism to suck the PBMC layers after conversion and positioning.

In order to achieve the purpose, the invention adopts the following technical scheme:

an apparatus for automatically extracting PBMCs, comprising the following components:

the device comprises a frame 1, wherein the frame 1 comprises a base 101, an upper supporting plate 102 and a side support (103 and a side support 104), two ends of the side supporting plate 103 are respectively connected with the base 101 and the upper supporting plate 102, and the side support 104 is arranged on the side supporting plate 103;

a suction mechanism provided on the side stand 104 for sucking the sample in the sample container;

a first elevating mechanism provided on the upper support plate 102 and the side support plate 103, including a second slider 10 that can be raised or lowered in a height direction of the side support plate 103;

the liquid transferring mechanism is arranged on the second slide block 10 and is used for transferring the sample in the sample container;

a second lifting mechanism, which is arranged on the upper support plate 102 and the side support plate 103, comprises a third slide block 12, and can also lift or descend along the height direction of the side support plate 103;

and the layered liquid level detection mechanism is arranged on the third slide block 12 and is used for detecting the layering condition of the liquid level of the sample.

The device, preferably, the pumping mechanism includes a liquid transferring pump 6, the liquid transferring pump 6 includes a liquid transferring motor 601, a lifting shaft screw 604 and a pumping barrel 602, an output end of the liquid transferring motor 601 is connected with the lifting shaft screw 604, the lifting shaft screw 604 is connected with a lower end of the pumping barrel 602, and a piston 603 is disposed in the pumping barrel 602.

The device, preferably, first elevating system still includes first elevator motor 3 and moves liquid screw rod 4, move liquid screw rod 4 both ends respectively with the base 101 with go up the backup pad 102 and connect, first elevator motor 3 sets up on the side backup pad 103, the output of first elevator motor 3 pass through the belt with move liquid screw rod 4 and connect, move liquid the last cover of screw rod 4 have can follow its axial slip second slider 10, second slider 10 is located go up backup pad 102 below of terminal surface.

The device, preferably, the pipetting mechanism comprises a catheter 5 and a first slide 9, the lower end of the first slide 9 is provided with a clamping part 901, the upper end of the first slide 9 is fixed on the second slide 10 and is connected with the first end of the catheter 5, and the second end of the catheter 5 is connected with the upper end of the suction cylinder 602.

The device, preferably, second elevating system still includes second elevator motor 13 and height finding screw rod 7, second elevator motor 13 sets up on the collateral branch backup pad 103, the both ends of height finding screw rod 7 respectively with the base 101 with go up the backup pad 102 and connect, the output of second elevator motor 13 pass through the belt with height finding screw rod 7 is connected, the cover has on the height finding screw rod 7 can follow its axial slip third slider 12, third slider 12 is located go up the below of backup pad 102 lower extreme face.

The device, preferably, the layering liquid level detection mechanism includes an optical fiber box 11, a first clamping leg 111 and a second clamping leg 112 are arranged at the lower end of the optical fiber box 11 and are parallel to the pipetting screw 4, a pair of paired holes are arranged on the first clamping leg 111 and the second clamping leg 112, and a transmitter and a receiver are respectively arranged in the paired holes.

The device is preferably provided with the height measuring screw 7 which is vertically parallel to the liquid transferring screw 4.

The device, preferably, be provided with two slide rails 105 on the side backup pad 103, second slider 10 with third slider 12 is provided with the spout respectively with slide rail 105 cooperatees.

Due to the adoption of the technical scheme, the invention has the following advantages:

1. the device respectively detects the positions of the cell layers according to different cell layers after layering and the difference of the light transmittance of liquid according to the additionally arranged layered liquid level detection mechanism, particularly a sensitive optical fiber detection method, and drives the pumping mechanism to pump the PBMC layer after conversion positioning;

2. the device has the functions of high detection precision, high extraction rate, stability, error reporting and the like, is suitable for replacing manual work to carry out batch extraction operation, and greatly reduces the operation intensity of experimenters.

Drawings

FIG. 1 is a schematic view of the overall structure of the apparatus of the present invention;

FIG. 2 is a schematic view of another angle overall structure of the device of the present invention;

the respective symbols in the figure are as follows:

1-a frame, 101-a base, 102-an upper supporting plate, 103-a side supporting plate, 104-a side bracket and 105-a slide rail; 2-gun head; 3-a first lift motor; 4-a pipetting screw; 5-a catheter; 6-liquid transferring pump, 601-liquid transferring motor, 602-suction cylinder, 603-piston, 604-lifting shaft screw; 7-height measuring screw; 8-a controller; 9-first slider, 901-clamping part; 10-a second slide; 11-fiber box, 111-first clamping leg, 112-second clamping leg; 12-a third slide; 13-second lifting motor.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, the technical solutions of the present invention are described clearly and completely below, and it is obvious that the described embodiments are some, not all embodiments of the present invention. All other embodiments, which can be obtained by a person skilled in the art without any inventive step based on the embodiments of the present invention, are within the scope of the present invention.