Reaction cavity module and micro-fluidic chip
阅读说明:本技术 反应腔模块及微流控芯片 (Reaction cavity module and micro-fluidic chip ) 是由 葛胜祥 张师音 苏晓崧 张东旭 闵小平 张建中 张雅 郭清顺 张军 夏宁邵 于 2018-08-22 设计创作,主要内容包括:本发明涉及一种反应腔模块及微流控芯片。其中,反应腔模块包括:模块主体;反应腔,设于所述模块主体内,所述反应腔的底部向下凸出;液流通道,设于所述模块主体内,其第一端经由所述反应腔的底部的最低端与所述反应腔连通,第二端与所述模块主体的外部连通;以及气流通道,设于所述模块主体内,其第一端经由所述反应腔的顶部与所述反应腔气体连通,其第二端与所述模块主体的外部气体连通。本发明中的反应腔可以做成大容量的容积空间,用于容纳反应试剂并为其提供反应空间支持。(The invention relates to a reaction cavity module and a micro-fluidic chip. Wherein, the reaction chamber module includes: a module body; the reaction cavity is arranged in the module main body, and the bottom of the reaction cavity protrudes downwards; the liquid flow channel is arranged in the module main body, the first end of the liquid flow channel is communicated with the reaction cavity through the lowest end of the bottom of the reaction cavity, and the second end of the liquid flow channel is communicated with the outside of the module main body; and the airflow channel is arranged in the module main body, the first end of the airflow channel is in gas communication with the reaction cavity through the top of the reaction cavity, and the second end of the airflow channel is in gas communication with the outside of the module main body. The reaction chamber of the present invention may be formed as a large volume space for holding and providing reaction space support for the reaction reagents.)
1. A reaction chamber module, comprising:
a module body (1);
the reaction cavity (2) is arranged in the module main body (1), and the bottom of the reaction cavity (2) protrudes downwards;
a liquid flow channel (3) arranged in the module main body (1), wherein a first end of the liquid flow channel is communicated with the reaction cavity (2) through the lowest end of the bottom of the reaction cavity (2), and a second end of the liquid flow channel is communicated with the outside of the module main body (1); and
and the gas flow channel (4) is arranged in the module main body (1), the first end of the gas flow channel is in gas communication with the reaction cavity (2) through the top of the reaction cavity (2), and the second end of the gas flow channel is in gas communication with the outside of the module main body (1).
2. A reaction chamber module according to claim 1, characterized by comprising an energy-conducting structure (5) arranged outside the module body (1) where the reaction chamber (2) is located for contacting a device (8) for providing vibrational energy into the reaction chamber (2) for transferring energy into the reaction chamber (2).
3. A reaction chamber module according to claim 1, wherein the reaction chamber (2) comprises a cylindrical reaction chamber, the radial direction of the cylindrical reaction chamber being the direction between the top and the bottom of the reaction chamber (2), and the axial direction of the cylindrical reaction chamber being the direction between opposite side portions of the reaction chamber (2).
4. A reactor chamber module according to claim 3, wherein the flow channel (3) comprises a channel section tangential to the bottom of the cylindrical reactor chamber and/or the gas flow channel (4) comprises a channel section tangential to the top of the cylindrical reactor chamber.
5. A reaction chamber module according to claim 3, characterized by comprising a buffer part (6), wherein one end of the buffer part (6) is tangential to the top of the cylindrical reaction chamber, and the other end is communicated with the cylindrical reaction chamber.
6. A reaction chamber module according to claim 3, characterized by comprising an energy conducting structure (5) arranged outside the module body (1) and on the central axis of the cylindrical reaction chamber for contacting a device (8) for providing vibrational energy into the cylindrical reaction chamber for transferring energy into the cylindrical reaction chamber.
7. A reaction chamber module according to claim 2 or 6, characterized in that the energy guiding structure (5) is cylindrical, conical or hemispherical.
8. A reaction chamber module according to claim 2 or 6, wherein the energy guiding structure (5) comprises an outwardly convex wall surface provided at a position corresponding to the reaction chamber (2) on one side of the module body (1).
9. A reaction chamber module according to claim 1, characterized by comprising a filter chamber (7) provided in the gas flow channel (4) and filled with a material for filtering gases.
10. A reaction chamber module according to claim 1, wherein more than one reaction chamber (2) is provided in the module body (1), and each reaction chamber (2) is provided with the flow channel (3) and the gas flow channel (4).
11. A microfluidic chip comprising the reaction chamber module according to any one of claims 1 to 10.
12. Microfluidic chip according to claim 11, comprising a device (8) for providing vibrational energy into the reaction chamber (2) in the reaction chamber module.
13. Microfluidic chip according to claim 12, characterised in that the device (8) for providing vibrational energy comprises an ultrasonic transducer, an eccentric vibrator or an electromagnetic transducer.
Technical Field
The invention relates to the field of microfluidic detection, in particular to a reaction cavity module and a microfluidic chip.
Background
Due to its high integration and strong automation, the microfluidic chip technology is increasingly applied to point-of-care testing (POCT) in clinical testing projects. In order to transplant the existing reagent system to the microfluidic platform, a reaction cavity with a corresponding large volume is required for supporting.
The problems with large volume reaction chambers are: 1) the excessive reaction cavity volume is not beneficial to the uniform input of the reagent, and the bubble blockage is easy to occur, so that the uneven distribution of the reaction system in the reaction cavity is caused; 2) the general large-volume reaction chamber is difficult to completely discharge waste liquid, so that the waste liquid in the reaction chamber is remained, the dosage of washing liquid is increased, and wrong detection results are easily caused.
At present, manufacturers of microfluidic detection systems at home and abroad mainly adopt a flexible reaction chamber or a micro reaction chamber to solve the problem. But the sample cracking efficiency of the flexible reaction cavity is lower, and the instrument control is more complex; the micro reaction cavity needs to be matched with a sample enrichment membrane with a patented technology for use, and the cost is extremely high.
Disclosure of Invention
One of the objectives of the present invention is to provide a reaction chamber module and a microfluidic chip which are beneficial to the waste liquid drainage.
To achieve the above object, the present invention provides a reaction chamber module, comprising: a module body; the reaction cavity is arranged in the module main body, and the bottom of the reaction cavity protrudes downwards; the liquid flow channel is arranged in the module main body, the first end of the liquid flow channel is communicated with the reaction cavity through the lowest end of the bottom of the reaction cavity, and the second end of the liquid flow channel is communicated with the outside of the module main body; and the airflow channel is arranged in the module main body, the first end of the airflow channel is in gas communication with the reaction cavity through the top of the reaction cavity, and the second end of the airflow channel is in gas communication with the outside of the module main body.
Optionally, the reaction cavity module comprises an energy guide structure, which is arranged at the outer side of the module body where the reaction cavity is located, and is used for contacting with a device for providing vibration energy into the reaction cavity so as to transfer the energy into the reaction cavity.
Optionally, the reaction chamber includes a cylindrical reaction chamber, a radial direction of the cylindrical reaction chamber is a direction between a top and a bottom of the reaction chamber, and an axial direction of the cylindrical reaction chamber is a direction between opposite side portions of the reaction chamber.
Optionally, the liquid flow channel comprises a channel section tangential to the bottom of the cylindrical reaction chamber, and/or the gas flow channel comprises a channel section tangential to the top of the cylindrical reaction chamber.
Optionally, the reaction cavity module comprises a buffer part, one end of the buffer part is tangent to the top of the cylindrical reaction cavity, and the other end of the buffer part is communicated with the cylindrical reaction cavity.
Optionally, the reaction chamber module comprises an energy guide structure, which is disposed outside the module body and on the central axis of the cylindrical reaction chamber, and is used for contacting with a device for providing vibration energy into the cylindrical reaction chamber to transfer energy into the cylindrical reaction chamber.
Optionally, the energy guiding structure is cylindrical, conical or hemispherical.
Optionally, the energy guiding structure includes a wall surface protruding outward and disposed on one side of the module body at a position corresponding to the reaction chamber.
Optionally, the reaction chamber module comprises a filter chamber disposed in the gas flow channel, and filled with a material for filtering gas.
Optionally, more than one reaction chamber is arranged in the module main body, and each reaction chamber is provided with the liquid flow channel and the gas flow channel.
In order to achieve the above object, the present invention provides a microfluidic chip, which includes the reaction chamber module.
Optionally, the microfluidic chip comprises a device for providing vibrational energy into a reaction chamber in said reaction chamber module.
Optionally, the device for providing vibrational energy comprises an ultrasonic transducer, an eccentric vibrator or an electromagnetic transducer.
Based on the technical scheme, the invention at least has the following beneficial effects:
in some embodiments, a reaction cavity is arranged in the module main body, the bottom of the reaction cavity protrudes downwards, waste liquid is discharged from the bottom of the reaction cavity, and the reaction cavity can be made into a large-capacity volume space for accommodating reaction reagents and providing reaction space support for the reaction reagents.
Drawings
The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this application, illustrate embodiment(s) of the invention and together with the description serve to explain the invention without limiting the invention. In the drawings:
fig. 1(a) is a schematic front view of a substrate of a reaction chamber module according to some embodiments of the invention.
Fig. 1(b) is a schematic rear view of a substrate of a reaction chamber module according to some embodiments of the invention.
FIG. 2 is a schematic diagram of an assembly of a reactor module according to some embodiments of the invention.
Fig. 3 is a schematic side view of a substrate according to some embodiments of the invention.
FIG. 4 is a schematic assembly diagram of a reaction chamber module according to another embodiment of the present invention.
Fig. 5(a) is a schematic diagram of a first energy guiding structure of a reaction chamber module according to some embodiments of the invention.
Fig. 5(b) is a schematic cross-sectional view of fig. 5 (a).
Fig. 6(a) is a schematic diagram of a second energy guiding structure of a reaction chamber module according to some embodiments of the invention.
Fig. 6(b) is a schematic cross-sectional view of fig. 6 (a).
Fig. 7(a) is a schematic diagram of a third energy guiding structure of a reaction chamber module according to some embodiments of the invention.
Fig. 7(b) is a schematic cross-sectional view of fig. 7 (a).
Fig. 8(a) is a schematic diagram of a fourth energy guiding structure of a reaction chamber module according to some embodiments of the invention.
Fig. 8(b) is a schematic cross-sectional view of fig. 8 (a).
FIG. 9 is a schematic diagram of a reaction chamber module including a buffer tank according to some embodiments of the invention.
FIG. 10 is a schematic diagram of a reactor module and apparatus for providing vibrational energy in accordance with certain embodiments of the present invention.
Fig. 11 is a partial cross-sectional schematic view of fig. 10.
The reference numbers in the drawings:
1-a module body; 11-a substrate; 12-a back plate;
2-a reaction chamber;
3-a flow channel; 31-a first via;
4-an airflow channel; 41-a second through hole;
5-an energy conducting structure;
6-a buffer part;
7-a filter chamber; 71-filtration of the filling;
8-devices for providing vibrational energy.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments. It is to be understood that the described embodiments are merely a few embodiments of the invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments of the present invention without any inventive step, are within the scope of the present invention.
In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc., indicate orientations or positional relationships based on those shown in the drawings, and are used merely for convenience in describing the present invention and for simplifying the description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore, should not be taken as limiting the scope of the present invention.
FIGS. 1-9 are schematic views of a reaction chamber module according to some embodiments.
In some embodiments, as shown in fig. 1(a), 1(b), the reaction chamber module comprises a
In some embodiments, the reaction cavity module comprises a
Because the bottom of the
In some embodiments, as shown in fig. 1(a), the reaction chamber module comprises a
In some embodiments, the reaction chamber module includes
The
In some embodiments, more than one
In some embodiments, as shown in FIG. 2, the
As shown in fig. 2, a surface of the
The
In some embodiments, as shown in fig. 4, the
In some embodiments, the
In some embodiments, the
In some embodiments, the
In some embodiments, as shown in fig. 9, the reaction chamber module includes a buffer part 6, the buffer part 6 is a cavity body disposed at the top of the
Alternatively, in some embodiments, the
In some embodiments, by providing the buffer part 6 at the top of the
In some embodiments, as shown in fig. 5 to 8, the reaction chamber module includes an
Under the condition of external force assistance of
Further, the
In some embodiments, the
In some embodiments, the
In some embodiments, the
In some embodiments, the
In some embodiments, the
It can be understood that the
As shown in fig. 10 and 11, when the reaction chamber module works, the
In some embodiments, as shown in fig. 3 and 4, the reaction chamber module comprises a
In some embodiments, along the flow of the gas flow in the
To sum up each embodiment,
The specific structure of the reaction chamber module will be described in detail below by way of specific examples of the reaction chamber module.
In this embodiment, the reaction cavity module has the functions of containing and uniformly mixing the reaction system and discharging the reaction waste liquid. The reaction cavity module comprises a
In this embodiment, the
In this particular embodiment, the
In this particular embodiment, the first and second through
In the first embodiment, the second through
Second embodiment on the basis of the first embodiment, a
And the mixing efficiency of the reagent in the reaction cavity is enhanced by adopting an external energy auxiliary mode. The external energy can be ultrasonic, mechanical vibration, eccentric vibrator, electromagnetic transducer and other mechanical transducers which can complete reciprocating vibration on one plane. An ultrasound probe is preferred.
In the third, fourth and fifth embodiments, in order to enhance the energy introduction of the reaction chamber and reduce the loss in the energy transfer process, the cylindrical, conical and hemispherical energy-conducting
In the sixth embodiment, the substrate surface of the reaction chamber is set to be a curved surface with a slight curvature as the
In the seventh embodiment, in order to suppress the reaction system from flowing upward along the edge of the cylindrical reaction chamber during the reaction process by capillary phenomenon and edge effect of the liquid, the upper half of the reaction chamber is modified to be square. The square is tangent with the two sides and the upper edge of the cylindrical reaction cavity, namely the height is 6mm, the length is 12mm, and the depth is 1 mm.
The reaction cavity module comprises an independent large-capacity reaction cavity, can be independently used, and can also be integrated into any microfluidic reaction detection chip to be used as a main or secondary reaction mechanism of the chip.
Some embodiments provide a microfluidic chip including the reaction chamber module described above.
Aiming at the problem that the existing microfluidic detection chip system is difficult to meet the requirement of a large-capacity reaction cavity, the reaction cavity module which is simple in structure and can be quickly formed through die sinking is provided. The reaction cavity in the reaction cavity module can realize seamless butt joint of reaction volumes above 200uL, the efficient reaction system is uniformly mixed, and reaction waste liquid is completely discharged. Meanwhile, the reaction cavity module can be integrated with any reaction chip, and has extremely strong universality; the mold can be opened, and the method has extremely high economy. The application of the reaction cavity module can greatly improve the clinical application value of the microfluidic detection chip.
In some embodiments, the microfluidic chip comprises a
In some embodiments, the
In the description of the present invention, it should be understood that the terms "first", "second", "third", etc. are used to define the components, and are used only for the convenience of distinguishing the components, and if not otherwise stated, the terms have no special meaning, and thus, should not be construed as limiting the scope of the present invention.
Finally, it should be noted that the above examples are only used to illustrate the technical solutions of the present invention and not to limit the same; although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art will understand that: modifications to the specific embodiments of the invention or equivalent substitutions for parts of the technical features may be made; without departing from the spirit of the present invention, it is intended to cover all aspects of the invention as defined by the appended claims.
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