阅读说明：本技术 一种防治纹党参根腐病的制剂及其制备方法 (Preparation for preventing and treating root rot of codonopsis pilosula and preparation method thereof ) 是由 王福元 王爱玉 蔺兴遥 于 2019-11-08 设计创作，主要内容包括：本发明公开了一种防治纹党参根腐病的制剂及其制备方法,属于农药技术领域。本发明的防治纹党参根腐病的制剂,由如下原料组成：黑曲霉菌粉、苜蓿粉、小球藻提取液、松果壳、尿素、半边莲提取物、升麻、芥子醇、没食子酸、4-羟基-苯甲酸、羽扇豆醇。本发明中在栽培纹党参前将制备的制剂与种子拌种后,纹党参的根腐病防效为97.3％～98.2％,抗寒力92.9％～94.7％,亩产量310～317kg/亩,比常规种植增产29.2％～32.1％,无农药残留,经济效益显著。(The invention discloses a preparation for preventing and treating root rot of pilose asiabell root and a preparation method thereof, belonging to the technical field of pesticides. The preparation for preventing and treating root rot of pilose asiabell root of the invention is composed of the following raw materials: aspergillus niger powder, alfalfa powder, chlorella extracting solution, pine nut shell, urea, Chinese lobelia extract, cimicifuga foetida, sinapyl alcohol, gallic acid, 4-hydroxy-benzoic acid and lupeol. According to the method, after the prepared preparation and seeds are mixed before the pilose asiabell root is cultivated, the root rot prevention effect of the pilose asiabell root is 97.3% -98.2%, the cold resistance is 92.9% -94.7%, the yield per mu is 310-317 kg/mu, the yield is increased by 29.2% -32.1% compared with that of conventional planting, no pesticide residue is generated, and the economic benefit is remarkable.)

1. A preparation for preventing and treating root rot of Wen radix codonopsis is characterized by comprising the following raw materials in parts by weight: 41-47 parts of Aspergillus niger powder, 27-39 parts of alfalfa powder, 29.5-39.5 parts of chlorella extract, 23.5-27.5 parts of pine cone shell, 21-27 parts of urea, 17-25 parts of Chinese lobelia extract, 17-25 parts of cimicifuga foetida, 14-22 parts of sinapyl alcohol, 11-17 parts of gallic acid, 6-10 parts of 4-hydroxy-benzoic acid and 3-7 parts of lupeol.

2. The preparation for preventing and treating root rot of pilose asiabell root according to claim 1, which is characterized by comprising the following raw materials in parts by weight: the traditional Chinese medicine composition comprises, by weight, 44 parts of aspergillus niger powder, 34.5 parts of chlorella extracting solution, 25.5 parts of pine cone shell, 24 parts of urea, 21 parts of Chinese lobelia extract, 21 parts of cimicifuga foetida, 18 parts of sinapyl alcohol, 14 parts of gallic acid, 8 parts of 4-hydroxy-benzoic acid and 5 parts of lupeol.

3. The preparation method of the preparation for preventing and treating root rot of codonopsis pilosula as claimed in claim 1, characterized by comprising the following steps:

1) culturing Aspergillus niger powder on sterile protein-free fermentation broth without protein powder to stabilize the Aspergillus niger powder, inoculating cold-hot processed broad bean powder on the Aspergillus niger powder, and performing solid state fermentation on the broad bean powder inoculated with the Aspergillus niger powder to obtain fermented broad bean powder X;

2) cleaning 1/4 cimicifugae rhizoma and herba Lobeliae chinensis extract, removing impurities, cutting into blocks with thickness of 1.2-1.5 cm, soaking in chlorella extractive solution and gallic acid for 35-40 min, uniformly placing on a tray, and puffing in an airflow puffing tank to obtain puffed material W; crushing the residual 3/4 cimicifugae rhizoma into 270-400 meshes of micro powder, adding 6-8 times of purified water, adjusting the pH to 6.4-7.0 by using gallic acid, heating to 45-60 ℃, and carrying out heat preservation and extraction for 40-50 min to obtain an extract R for later use;

3) crushing pine cone shells, homogenizing the crushed pine cone shells and 4-hydroxy-benzoic acid at 45-65 ℃ to obtain a mixed homogeneous system, adding the mixed homogeneous system into a chloroform-methanol mixed solution with the weight 6-11 times of that of the mixed homogeneous system, and fully stirring the mixed homogeneous system for 8-14 hours at 4-17 ℃ to obtain pine cone shell slurry; filtering the stirred pine nut shell slurry by a filter membrane, and then rotatably evaporating the filtrate to dryness; extracting the evaporated material again by chloroform-methanol-water with volume ratio of 4: 3: 1.8, standing for layering, and collecting chloroform layer or evaporating chloroform layer to dryness to obtain mixture T; and then mixing the fermented broad bean powder X, the puffed material W, the extract R, the mixture T, urea, alfalfa powder, sinapyl alcohol, 4-hydroxy-benzoic acid and lupeol to obtain the preparation for preventing and treating the root rot of the Codonopsis pilosula.

4. The preparation method of the preparation for preventing and treating root rot of pilose asiabell root according to claim 3, characterized in that the temperature of heat treatment in step 1) is maintained at 68-120 ℃ for 15min, the temperature of cold treatment is maintained at 35-42 ℃ for 25-30 min, and the temperature of solid fermentation is maintained at 46-55 ℃ for 14-30 h.

5. The preparation method of the preparation for preventing and treating root rot of codonopsis pilosula in claim 3 or 4, wherein the protein-containing fermentation broth in the step 1) comprises 0.09-1.2 wt% of granulated sugar, 0.004-0.1 wt% of acetic acid, 0.07-1.3 wt% of cyclohexane carboxylate and 0.08-2.9 wt% of protein powder based on the total weight of the protein-containing fermentation broth.

6. The preparation method of the preparation for preventing and treating root rot of pilose asiabell root according to claim 3, characterized in that the air flow puffing temperature in the step 2) is controlled to 82-86 ℃, and the vacuum degree is controlled to 0.12-0.18 MPa.

Technical Field

The invention belongs to the technical field of pesticides, and particularly relates to a preparation for preventing and treating root rot of codonopsis pilosula and a preparation method thereof.

Background

Wen radix Codonopsis is dried root of Angelica gigas nakai (Angelica pubescens Maxim. f. bisraratashan et Yuan) of Umbelliferae. Digging when seedlings just sprout in the early spring or when stems and leaves wither in the late autumn, removing fibrous roots and silt, drying to be semi-dry, piling for 2-3 days, softening and then drying to be completely dry. The surface is dark brown or dark brown, has longitudinal wrinkles, and has transverse long skin hole-like protrusions and slightly protruding fine root marks. Hard, soft when affected by moisture, grey-white cross section skin with a lot of scattered brown oil chambers, grey-yellow to yellow-brown wood, forming ring-brown color. Has special fragrance, and is bitter, pungent and slightly numb tongue. Contains osthole (C) calculated on dried product₁₅H₁₆O₃) Not less than 0.50% of angelate containing dihydrocarveol (C)₁₉H₂₀O₅) Not less than 0.080%.

The root rot of the Wen dangshen is mainly controlled by methods of intertillage weeding, drainage, selection of disease-free seedlings, seed soaking in 1:150 Bordeaux mixture, airing and sowing in the prior art, or 1000 times of 50 percent carbendazim wettable powder or 50 percent vertical withering water purifying agent 1500g/hm at the early stage of disease²Spraying 750kg of water for prevention and treatment, wherein the spraying is carried out for 1 time every 7 days and is carried out for 3-4 times continuously. However, the method has incomplete effect of preventing and treating root rot, a part of pesticide is remained in crops, and the method has certain limitation, and no effective preparation for preventing and treating root rot of codonopsis pilosula is available at present. Aiming at the defects, the prepared preparation can prevent and treat root rot and enhance the cold resistance of the radix codonopsis pilosulae adapting to the environment and the crop yield.

Disclosure of Invention

The invention aims to provide a preparation for preventing and treating root rot of pilose asiabell root and a preparation method thereof, wherein the preparation can be used for preventing and treating the root rot of pilose asiabell root and enhancing the cold resistance and crop yield of pilose asiabell root.

In order to achieve the purpose, the invention is realized by adopting the following technical scheme.

A preparation for preventing and treating root rot of Wen radix codonopsis comprises the following raw materials in parts by weight: 41-47 parts of Aspergillus niger powder, 27-39 parts of alfalfa powder, 29.5-39.5 parts of chlorella extract, 23.5-27.5 parts of pine cone shell, 21-27 parts of urea, 17-25 parts of Chinese lobelia extract, 17-25 parts of cimicifuga foetida, 14-22 parts of sinapyl alcohol, 11-17 parts of gallic acid, 6-10 parts of 4-hydroxy-benzoic acid and 3-7 parts of lupeol.

Further, the preparation for preventing and treating root rot of codonopsis pilosula consists of the following raw materials in parts by weight: 44 parts of T-3 strain, 34.5 parts of chlorella extract, 25.5 parts of pine cone shell, 24 parts of urea, 21 parts of Chinese lobelia extract, 21 parts of cimicifuga foetida, 18 parts of sinapyl alcohol, 14 parts of gallic acid, 8 parts of 4-hydroxy-benzoic acid, 5 parts of lupeol and 95 parts of water.

The preparation method of the preparation for preventing and treating root rot of codonopsis pilosula comprises the following steps:

1) culturing Aspergillus niger powder on sterile protein-free fermentation broth without protein powder to stabilize the Aspergillus niger powder, inoculating cold-hot processed broad bean powder thereon, and performing solid state fermentation on the broad bean powder inoculated with the T-3 strain to obtain fermented broad bean powder X;

2) cleaning 1/4 cimicifugae rhizoma and herba Lobeliae chinensis extract, removing impurities, cutting into blocks with thickness of 1.2-1.5 cm, soaking in chlorella extractive solution and gallic acid for 35-40 min, uniformly placing on a tray, and puffing in an airflow puffing tank to obtain puffed material W; crushing the residual 3/4 cimicifugae rhizoma into 270-400 meshes of micro powder, adding 6-8 times of purified water, adjusting the pH to 6.4-7.0 by using gallic acid, heating to 45-60 ℃, and carrying out heat preservation and extraction for 40-50 min to obtain an extract R for later use;

3) crushing pine cone shells, homogenizing the crushed pine cone shells and 4-hydroxy-benzoic acid at 45-65 ℃ to obtain a mixed homogeneous system, adding the mixed homogeneous system into a chloroform-methanol mixed solution with the weight 6-11 times of that of the mixed homogeneous system, and fully stirring the mixed homogeneous system for 8-14 hours at 4-17 ℃ to obtain pine cone shell slurry; filtering the stirred pine nut shell slurry by a filter membrane, and then rotatably evaporating the filtrate to dryness; extracting the evaporated material again by chloroform-methanol-water with volume ratio of 4: 3: 1.8, standing for layering, and collecting chloroform layer or evaporating chloroform layer to dryness to obtain mixture T; and then mixing the fermented broad bean powder X, the puffed material W, the extract R, the mixture T, urea, alfalfa powder, sinapyl alcohol, 4-hydroxy-benzoic acid and lupeol to obtain the preparation for preventing and treating the root rot of the Codonopsis pilosula.

Further, the temperature of the heat treatment in the step 1) is maintained at 68-120 ℃ for 15min, the temperature of the cold treatment is maintained at 35-42 ℃ for 25-30 min, and the temperature of the solid fermentation is maintained at 46-55 ℃ for 14-30 h.

Further, the protein-containing fermentation broth in the step 1) comprises 0.09-1.2 wt% of granulated sugar, 0.004-0.1 wt% of acetic acid, 0.07-1.3 wt% of cyclohexane carboxylate and 0.08-2.9 wt% of protein powder based on the total weight of the protein-containing fermentation broth.

Further, the air flow puffing temperature in the step 2) is controlled to be 82-86 ℃, and the vacuum degree is controlled to be 0.12-0.18 MPa.

Compared with the prior art, the invention has the beneficial technical effects that:

1) the invention can reduce the prevention effect on the germination rate and the root rot of the codonopsis pilosula seeds in the absence of any one of aspergillus niger bacterial powder and chlorella extract, the germination rate of the seeds is 96.3% under normal conditions, the prevention effect on the root rot is 97.7%, the germination rate and the root rot of the seeds are respectively reduced to 76.7% and 77.1% in the absence of the T-3 strain, the germination rate and the root rot are respectively reduced to 74.4% and 74.9% in the absence of the chlorella extract, and the germination rate and the root rot are respectively reduced to 59.2% and 53.8% in the absence of the chlorella extract.

2) According to the method, urea is not used, the acre yield of the codonopsis pilosula is obviously reduced, the reduction rate is 18%, the acre yield of the codonopsis pilosula is reduced by more or less when the content of the urea is increased or reduced, and the reduction rate is 10.1% -11.7%.

3) According to the method, after the prepared preparation and seeds are mixed before the pilose asiabell root is cultivated, the root rot prevention effect of the pilose asiabell root is 97.3% -98.2%, the cold resistance is 92.9% -94.7%, the yield per mu is 310-317 kg/mu, the yield is increased by 29.2% -32.1% compared with that of conventional planting, no pesticide residue is generated, and the economic benefit is remarkable.

Detailed Description

The technical solution of the present invention will be further specifically described below by way of specific examples. It is to be understood that the embodiments of the present invention are not limited to the following embodiments, and that any changes and/or modifications may be made to the present invention within the scope of the present invention.